Ghrelin Facilitates GLUT2-, SGLT1-and SGLT2-mediated Intestinal Glucose Transport in Goldfish (Carassius auratus)

Autores
Blanco, Ayelén Melisa; Bertucci, Juan Ignacio; Ramesh, Naresh; Delgado, Mariá Jesús; Valenciano, Ana Isabel; Unniappan, Suraj
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Glucose homeostasis is an important biological process that involves a variety of regulatory mechanisms. This study aimed to determine whether ghrelin, a multifunctional gut-brain hormone, modulates intestinal glucose transport in goldfish (Carassius auratus). Three intestinal glucose transporters, the facilitative glucose transporter 2 (GLUT2), and the sodium/glucose co-transporters 1 (SGLT1) and 2 (SGLT2), were studied. Immunostaining of intestinal sections found colocalization of ghrelin and GLUT2 and SGLT2 in mucosal cells. Some cells containing GLUT2, SGLT1 and SGLT2 coexpressed the ghrelin/growth hormone secretagogue receptor 1a (GHS-R1a). Intraperitoneal glucose administration led to a significant increase in serum ghrelin levels, as well as an upregulation of intestinal preproghrelin, ghrelin O-acyltransferase and ghs-r1 expression. In vivo and in vitro ghrelin treatment caused a concentration- and time-dependent modulation (mainly stimulatory) of GLUT2, SGLT1 and SGLT2. These effects were abolished by the GHS-R1a antagonist [D-Lys3]-GHRP-6 and the phospholipase C inhibitor U73122, suggesting that ghrelin actions on glucose transporters are mediated by GHS-R1a via the PLC/PKC signaling pathway. Finally, ghrelin stimulated the translocation of GLUT2 into the plasma membrane of goldfish primary intestinal cells. Overall, data reported here indicate an important role for ghrelin in the modulation of glucoregulatory machinery and glucose homeostasis in fish.
Fil: Blanco, Ayelén Melisa. Universidad Complutense de Madrid; España. University of Saskatchewan; Canadá
Fil: Bertucci, Juan Ignacio. University of Saskatchewan; Canadá. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Ramesh, Naresh. University of Saskatchewan; Canadá
Fil: Delgado, Mariá Jesús. Universidad Complutense de Madrid; España
Fil: Valenciano, Ana Isabel. Universidad Complutense de Madrid; España
Fil: Unniappan, Suraj. University of Saskatchewan; Canadá
Materia
ghrelin
fish
glucose transportes
glucose homeostasis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/49680
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/49680
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Ghrelin Facilitates GLUT2-, SGLT1-and SGLT2-mediated Intestinal Glucose Transport in Goldfish (Carassius auratus)Blanco, Ayelén MelisaBertucci, Juan IgnacioRamesh, NareshDelgado, Mariá JesúsValenciano, Ana IsabelUnniappan, Surajghrelinfishglucose transportesglucose homeostasishttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Glucose homeostasis is an important biological process that involves a variety of regulatory mechanisms. This study aimed to determine whether ghrelin, a multifunctional gut-brain hormone, modulates intestinal glucose transport in goldfish (Carassius auratus). Three intestinal glucose transporters, the facilitative glucose transporter 2 (GLUT2), and the sodium/glucose co-transporters 1 (SGLT1) and 2 (SGLT2), were studied. Immunostaining of intestinal sections found colocalization of ghrelin and GLUT2 and SGLT2 in mucosal cells. Some cells containing GLUT2, SGLT1 and SGLT2 coexpressed the ghrelin/growth hormone secretagogue receptor 1a (GHS-R1a). Intraperitoneal glucose administration led to a significant increase in serum ghrelin levels, as well as an upregulation of intestinal preproghrelin, ghrelin O-acyltransferase and ghs-r1 expression. In vivo and in vitro ghrelin treatment caused a concentration- and time-dependent modulation (mainly stimulatory) of GLUT2, SGLT1 and SGLT2. These effects were abolished by the GHS-R1a antagonist [D-Lys3]-GHRP-6 and the phospholipase C inhibitor U73122, suggesting that ghrelin actions on glucose transporters are mediated by GHS-R1a via the PLC/PKC signaling pathway. Finally, ghrelin stimulated the translocation of GLUT2 into the plasma membrane of goldfish primary intestinal cells. Overall, data reported here indicate an important role for ghrelin in the modulation of glucoregulatory machinery and glucose homeostasis in fish.Fil: Blanco, Ayelén Melisa. Universidad Complutense de Madrid; España. University of Saskatchewan; CanadáFil: Bertucci, Juan Ignacio. University of Saskatchewan; Canadá. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Ramesh, Naresh. University of Saskatchewan; CanadáFil: Delgado, Mariá Jesús. Universidad Complutense de Madrid; EspañaFil: Valenciano, Ana Isabel. Universidad Complutense de Madrid; EspañaFil: Unniappan, Suraj. University of Saskatchewan; CanadáNature Publishing Group2017-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/49680Blanco, Ayelén Melisa; Bertucci, Juan Ignacio; Ramesh, Naresh; Delgado, Mariá Jesús; Valenciano, Ana Isabel; et al.; Ghrelin Facilitates GLUT2-, SGLT1-and SGLT2-mediated Intestinal Glucose Transport in Goldfish (Carassius auratus); Nature Publishing Group; Scientific Reports; 7; 3-2017; 1-162045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1038/srep45024info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/srep45024info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:54:06Zoai:ri.conicet.gov.ar:11336/49680instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:54:07.156CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Ghrelin Facilitates GLUT2-, SGLT1-and SGLT2-mediated Intestinal Glucose Transport in Goldfish (Carassius auratus)
title Ghrelin Facilitates GLUT2-, SGLT1-and SGLT2-mediated Intestinal Glucose Transport in Goldfish (Carassius auratus)
spellingShingle Ghrelin Facilitates GLUT2-, SGLT1-and SGLT2-mediated Intestinal Glucose Transport in Goldfish (Carassius auratus)
Blanco, Ayelén Melisa
ghrelin
fish
glucose transportes
glucose homeostasis
title_short Ghrelin Facilitates GLUT2-, SGLT1-and SGLT2-mediated Intestinal Glucose Transport in Goldfish (Carassius auratus)
title_full Ghrelin Facilitates GLUT2-, SGLT1-and SGLT2-mediated Intestinal Glucose Transport in Goldfish (Carassius auratus)
title_fullStr Ghrelin Facilitates GLUT2-, SGLT1-and SGLT2-mediated Intestinal Glucose Transport in Goldfish (Carassius auratus)
title_full_unstemmed Ghrelin Facilitates GLUT2-, SGLT1-and SGLT2-mediated Intestinal Glucose Transport in Goldfish (Carassius auratus)
title_sort Ghrelin Facilitates GLUT2-, SGLT1-and SGLT2-mediated Intestinal Glucose Transport in Goldfish (Carassius auratus)
dc.creator.none.fl_str_mv Blanco, Ayelén Melisa
Bertucci, Juan Ignacio
Ramesh, Naresh
Delgado, Mariá Jesús
Valenciano, Ana Isabel
Unniappan, Suraj
author Blanco, Ayelén Melisa
author_facet Blanco, Ayelén Melisa
Bertucci, Juan Ignacio
Ramesh, Naresh
Delgado, Mariá Jesús
Valenciano, Ana Isabel
Unniappan, Suraj
author_role author
author2 Bertucci, Juan Ignacio
Ramesh, Naresh
Delgado, Mariá Jesús
Valenciano, Ana Isabel
Unniappan, Suraj
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ghrelin
fish
glucose transportes
glucose homeostasis
topic ghrelin
fish
glucose transportes
glucose homeostasis
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Glucose homeostasis is an important biological process that involves a variety of regulatory mechanisms. This study aimed to determine whether ghrelin, a multifunctional gut-brain hormone, modulates intestinal glucose transport in goldfish (Carassius auratus). Three intestinal glucose transporters, the facilitative glucose transporter 2 (GLUT2), and the sodium/glucose co-transporters 1 (SGLT1) and 2 (SGLT2), were studied. Immunostaining of intestinal sections found colocalization of ghrelin and GLUT2 and SGLT2 in mucosal cells. Some cells containing GLUT2, SGLT1 and SGLT2 coexpressed the ghrelin/growth hormone secretagogue receptor 1a (GHS-R1a). Intraperitoneal glucose administration led to a significant increase in serum ghrelin levels, as well as an upregulation of intestinal preproghrelin, ghrelin O-acyltransferase and ghs-r1 expression. In vivo and in vitro ghrelin treatment caused a concentration- and time-dependent modulation (mainly stimulatory) of GLUT2, SGLT1 and SGLT2. These effects were abolished by the GHS-R1a antagonist [D-Lys3]-GHRP-6 and the phospholipase C inhibitor U73122, suggesting that ghrelin actions on glucose transporters are mediated by GHS-R1a via the PLC/PKC signaling pathway. Finally, ghrelin stimulated the translocation of GLUT2 into the plasma membrane of goldfish primary intestinal cells. Overall, data reported here indicate an important role for ghrelin in the modulation of glucoregulatory machinery and glucose homeostasis in fish.
Fil: Blanco, Ayelén Melisa. Universidad Complutense de Madrid; España. University of Saskatchewan; Canadá
Fil: Bertucci, Juan Ignacio. University of Saskatchewan; Canadá. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Ramesh, Naresh. University of Saskatchewan; Canadá
Fil: Delgado, Mariá Jesús. Universidad Complutense de Madrid; España
Fil: Valenciano, Ana Isabel. Universidad Complutense de Madrid; España
Fil: Unniappan, Suraj. University of Saskatchewan; Canadá
description Glucose homeostasis is an important biological process that involves a variety of regulatory mechanisms. This study aimed to determine whether ghrelin, a multifunctional gut-brain hormone, modulates intestinal glucose transport in goldfish (Carassius auratus). Three intestinal glucose transporters, the facilitative glucose transporter 2 (GLUT2), and the sodium/glucose co-transporters 1 (SGLT1) and 2 (SGLT2), were studied. Immunostaining of intestinal sections found colocalization of ghrelin and GLUT2 and SGLT2 in mucosal cells. Some cells containing GLUT2, SGLT1 and SGLT2 coexpressed the ghrelin/growth hormone secretagogue receptor 1a (GHS-R1a). Intraperitoneal glucose administration led to a significant increase in serum ghrelin levels, as well as an upregulation of intestinal preproghrelin, ghrelin O-acyltransferase and ghs-r1 expression. In vivo and in vitro ghrelin treatment caused a concentration- and time-dependent modulation (mainly stimulatory) of GLUT2, SGLT1 and SGLT2. These effects were abolished by the GHS-R1a antagonist [D-Lys3]-GHRP-6 and the phospholipase C inhibitor U73122, suggesting that ghrelin actions on glucose transporters are mediated by GHS-R1a via the PLC/PKC signaling pathway. Finally, ghrelin stimulated the translocation of GLUT2 into the plasma membrane of goldfish primary intestinal cells. Overall, data reported here indicate an important role for ghrelin in the modulation of glucoregulatory machinery and glucose homeostasis in fish.
publishDate 2017
dc.date.none.fl_str_mv 2017-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/49680
Blanco, Ayelén Melisa; Bertucci, Juan Ignacio; Ramesh, Naresh; Delgado, Mariá Jesús; Valenciano, Ana Isabel; et al.; Ghrelin Facilitates GLUT2-, SGLT1-and SGLT2-mediated Intestinal Glucose Transport in Goldfish (Carassius auratus); Nature Publishing Group; Scientific Reports; 7; 3-2017; 1-16
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/49680
identifier_str_mv Blanco, Ayelén Melisa; Bertucci, Juan Ignacio; Ramesh, Naresh; Delgado, Mariá Jesús; Valenciano, Ana Isabel; et al.; Ghrelin Facilitates GLUT2-, SGLT1-and SGLT2-mediated Intestinal Glucose Transport in Goldfish (Carassius auratus); Nature Publishing Group; Scientific Reports; 7; 3-2017; 1-16
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1038/srep45024
info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/srep45024
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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