Aging-induced changes in 24-h rhythms of mitogenic responses, lymphocyte subset populations and neurotransmitter and amino acid content in rat submaxillary lymph nodes during Freun...

Autores
Garcia Bonacho, M.; Cardinali, Daniel Pedro; Castrillón, P.; Cutrera, Rodolfo Angel; Esquifino, A.I.
Año de publicación
2001
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In young (two months) and aged (18 months) male rats injected s.c. with Freund´s adjuvant or adjuvant´s vehicle 18 days earlier, 24-h variations in mitogenic responses, lymphocyte subsets and monoamine and amino acid content were examined in submaxillary lymph nodes. Mitogenic responses to concanavalin A (Con A) and lipopolysaccharide (LPS) were higher during the light phase of daily photoperiod. Old rats exhibited a suppressed or impaired mitogenic response to Con A but not to LPS. Acrophases of 24-h rhythm in lymphocyte subset populations in submaxillary lymph nodes were: 18:37-19:44h (B cells), 09:00-10:08h (T and CD4(+) cells) and 12:19-15:58h (CD8(+) cells). Aging augmented B cells and decreased T, CD4(+) and CD8(+) cells. Significant correlations were found between Con A activity and T cells, between lymph node 5HT content and B, T and CD8(+) lymphocytes, and between lymph node 5HT and taurine and GABA content. Aging increased lymph node 5HT content but did not modify NE content. Lymph node concentration of aspartate, glutamate and taurine was higher at night while that of GABA attained peak values at late afternoon. Old rats injected with Freund´s adjuvant showed a higher mean value (glutamate) and smaller amplitude (glutamate, taurine) than their respective young controls. The results further document the effects of aging on the chronobiology of the immune system.
Fil: Garcia Bonacho, M.. Universidad Complutense de Madrid; España
Fil: Cardinali, Daniel Pedro. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Castrillón, P.. Universidad Complutense de Madrid; España
Fil: Cutrera, Rodolfo Angel. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Esquifino, A.I.. Universidad Complutense de Madrid; España
Materia
AGING
ASPARTATE
CIRCADIAN RHYTHMS
CONCANAVALIN A
FREUND'S ADJUVANT
GABA
GLUTAMATE
LIPOPOLYSACCHARIDE
LYMPHOCYTE SUBSETS
NOREPINEPHRINE
SEROTONIN
SUBMAXILLARY LYMPH NODES
TAURINE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/152205
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/152205
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Aging-induced changes in 24-h rhythms of mitogenic responses, lymphocyte subset populations and neurotransmitter and amino acid content in rat submaxillary lymph nodes during Freund's adjuvant arthritisGarcia Bonacho, M.Cardinali, Daniel PedroCastrillón, P.Cutrera, Rodolfo AngelEsquifino, A.I.AGINGASPARTATECIRCADIAN RHYTHMSCONCANAVALIN AFREUND'S ADJUVANTGABAGLUTAMATELIPOPOLYSACCHARIDELYMPHOCYTE SUBSETSNOREPINEPHRINESEROTONINSUBMAXILLARY LYMPH NODESTAURINEhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3In young (two months) and aged (18 months) male rats injected s.c. with Freund´s adjuvant or adjuvant´s vehicle 18 days earlier, 24-h variations in mitogenic responses, lymphocyte subsets and monoamine and amino acid content were examined in submaxillary lymph nodes. Mitogenic responses to concanavalin A (Con A) and lipopolysaccharide (LPS) were higher during the light phase of daily photoperiod. Old rats exhibited a suppressed or impaired mitogenic response to Con A but not to LPS. Acrophases of 24-h rhythm in lymphocyte subset populations in submaxillary lymph nodes were: 18:37-19:44h (B cells), 09:00-10:08h (T and CD4(+) cells) and 12:19-15:58h (CD8(+) cells). Aging augmented B cells and decreased T, CD4(+) and CD8(+) cells. Significant correlations were found between Con A activity and T cells, between lymph node 5HT content and B, T and CD8(+) lymphocytes, and between lymph node 5HT and taurine and GABA content. Aging increased lymph node 5HT content but did not modify NE content. Lymph node concentration of aspartate, glutamate and taurine was higher at night while that of GABA attained peak values at late afternoon. Old rats injected with Freund´s adjuvant showed a higher mean value (glutamate) and smaller amplitude (glutamate, taurine) than their respective young controls. The results further document the effects of aging on the chronobiology of the immune system.Fil: Garcia Bonacho, M.. Universidad Complutense de Madrid; EspañaFil: Cardinali, Daniel Pedro. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Castrillón, P.. Universidad Complutense de Madrid; EspañaFil: Cutrera, Rodolfo Angel. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Esquifino, A.I.. Universidad Complutense de Madrid; EspañaPergamon-Elsevier Science Ltd2001-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/152205Garcia Bonacho, M.; Cardinali, Daniel Pedro; Castrillón, P.; Cutrera, Rodolfo Angel; Esquifino, A.I.; Aging-induced changes in 24-h rhythms of mitogenic responses, lymphocyte subset populations and neurotransmitter and amino acid content in rat submaxillary lymph nodes during Freund's adjuvant arthritis; Pergamon-Elsevier Science Ltd; Experimental Gerontology; 36; 2; 2-2001; 267-2820531-5565CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/s0531-5565(00)00211-4info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0531556500002114info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:09:07Zoai:ri.conicet.gov.ar:11336/152205instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:09:08.067CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Aging-induced changes in 24-h rhythms of mitogenic responses, lymphocyte subset populations and neurotransmitter and amino acid content in rat submaxillary lymph nodes during Freund's adjuvant arthritis
title Aging-induced changes in 24-h rhythms of mitogenic responses, lymphocyte subset populations and neurotransmitter and amino acid content in rat submaxillary lymph nodes during Freund's adjuvant arthritis
spellingShingle Aging-induced changes in 24-h rhythms of mitogenic responses, lymphocyte subset populations and neurotransmitter and amino acid content in rat submaxillary lymph nodes during Freund's adjuvant arthritis
Garcia Bonacho, M.
AGING
ASPARTATE
CIRCADIAN RHYTHMS
CONCANAVALIN A
FREUND'S ADJUVANT
GABA
GLUTAMATE
LIPOPOLYSACCHARIDE
LYMPHOCYTE SUBSETS
NOREPINEPHRINE
SEROTONIN
SUBMAXILLARY LYMPH NODES
TAURINE
title_short Aging-induced changes in 24-h rhythms of mitogenic responses, lymphocyte subset populations and neurotransmitter and amino acid content in rat submaxillary lymph nodes during Freund's adjuvant arthritis
title_full Aging-induced changes in 24-h rhythms of mitogenic responses, lymphocyte subset populations and neurotransmitter and amino acid content in rat submaxillary lymph nodes during Freund's adjuvant arthritis
title_fullStr Aging-induced changes in 24-h rhythms of mitogenic responses, lymphocyte subset populations and neurotransmitter and amino acid content in rat submaxillary lymph nodes during Freund's adjuvant arthritis
title_full_unstemmed Aging-induced changes in 24-h rhythms of mitogenic responses, lymphocyte subset populations and neurotransmitter and amino acid content in rat submaxillary lymph nodes during Freund's adjuvant arthritis
title_sort Aging-induced changes in 24-h rhythms of mitogenic responses, lymphocyte subset populations and neurotransmitter and amino acid content in rat submaxillary lymph nodes during Freund's adjuvant arthritis
dc.creator.none.fl_str_mv Garcia Bonacho, M.
Cardinali, Daniel Pedro
Castrillón, P.
Cutrera, Rodolfo Angel
Esquifino, A.I.
author Garcia Bonacho, M.
author_facet Garcia Bonacho, M.
Cardinali, Daniel Pedro
Castrillón, P.
Cutrera, Rodolfo Angel
Esquifino, A.I.
author_role author
author2 Cardinali, Daniel Pedro
Castrillón, P.
Cutrera, Rodolfo Angel
Esquifino, A.I.
author2_role author
author
author
author
dc.subject.none.fl_str_mv AGING
ASPARTATE
CIRCADIAN RHYTHMS
CONCANAVALIN A
FREUND'S ADJUVANT
GABA
GLUTAMATE
LIPOPOLYSACCHARIDE
LYMPHOCYTE SUBSETS
NOREPINEPHRINE
SEROTONIN
SUBMAXILLARY LYMPH NODES
TAURINE
topic AGING
ASPARTATE
CIRCADIAN RHYTHMS
CONCANAVALIN A
FREUND'S ADJUVANT
GABA
GLUTAMATE
LIPOPOLYSACCHARIDE
LYMPHOCYTE SUBSETS
NOREPINEPHRINE
SEROTONIN
SUBMAXILLARY LYMPH NODES
TAURINE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv In young (two months) and aged (18 months) male rats injected s.c. with Freund´s adjuvant or adjuvant´s vehicle 18 days earlier, 24-h variations in mitogenic responses, lymphocyte subsets and monoamine and amino acid content were examined in submaxillary lymph nodes. Mitogenic responses to concanavalin A (Con A) and lipopolysaccharide (LPS) were higher during the light phase of daily photoperiod. Old rats exhibited a suppressed or impaired mitogenic response to Con A but not to LPS. Acrophases of 24-h rhythm in lymphocyte subset populations in submaxillary lymph nodes were: 18:37-19:44h (B cells), 09:00-10:08h (T and CD4(+) cells) and 12:19-15:58h (CD8(+) cells). Aging augmented B cells and decreased T, CD4(+) and CD8(+) cells. Significant correlations were found between Con A activity and T cells, between lymph node 5HT content and B, T and CD8(+) lymphocytes, and between lymph node 5HT and taurine and GABA content. Aging increased lymph node 5HT content but did not modify NE content. Lymph node concentration of aspartate, glutamate and taurine was higher at night while that of GABA attained peak values at late afternoon. Old rats injected with Freund´s adjuvant showed a higher mean value (glutamate) and smaller amplitude (glutamate, taurine) than their respective young controls. The results further document the effects of aging on the chronobiology of the immune system.
Fil: Garcia Bonacho, M.. Universidad Complutense de Madrid; España
Fil: Cardinali, Daniel Pedro. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Castrillón, P.. Universidad Complutense de Madrid; España
Fil: Cutrera, Rodolfo Angel. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Esquifino, A.I.. Universidad Complutense de Madrid; España
description In young (two months) and aged (18 months) male rats injected s.c. with Freund´s adjuvant or adjuvant´s vehicle 18 days earlier, 24-h variations in mitogenic responses, lymphocyte subsets and monoamine and amino acid content were examined in submaxillary lymph nodes. Mitogenic responses to concanavalin A (Con A) and lipopolysaccharide (LPS) were higher during the light phase of daily photoperiod. Old rats exhibited a suppressed or impaired mitogenic response to Con A but not to LPS. Acrophases of 24-h rhythm in lymphocyte subset populations in submaxillary lymph nodes were: 18:37-19:44h (B cells), 09:00-10:08h (T and CD4(+) cells) and 12:19-15:58h (CD8(+) cells). Aging augmented B cells and decreased T, CD4(+) and CD8(+) cells. Significant correlations were found between Con A activity and T cells, between lymph node 5HT content and B, T and CD8(+) lymphocytes, and between lymph node 5HT and taurine and GABA content. Aging increased lymph node 5HT content but did not modify NE content. Lymph node concentration of aspartate, glutamate and taurine was higher at night while that of GABA attained peak values at late afternoon. Old rats injected with Freund´s adjuvant showed a higher mean value (glutamate) and smaller amplitude (glutamate, taurine) than their respective young controls. The results further document the effects of aging on the chronobiology of the immune system.
publishDate 2001
dc.date.none.fl_str_mv 2001-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/152205
Garcia Bonacho, M.; Cardinali, Daniel Pedro; Castrillón, P.; Cutrera, Rodolfo Angel; Esquifino, A.I.; Aging-induced changes in 24-h rhythms of mitogenic responses, lymphocyte subset populations and neurotransmitter and amino acid content in rat submaxillary lymph nodes during Freund's adjuvant arthritis; Pergamon-Elsevier Science Ltd; Experimental Gerontology; 36; 2; 2-2001; 267-282
0531-5565
CONICET Digital
CONICET
url http://hdl.handle.net/11336/152205
identifier_str_mv Garcia Bonacho, M.; Cardinali, Daniel Pedro; Castrillón, P.; Cutrera, Rodolfo Angel; Esquifino, A.I.; Aging-induced changes in 24-h rhythms of mitogenic responses, lymphocyte subset populations and neurotransmitter and amino acid content in rat submaxillary lymph nodes during Freund's adjuvant arthritis; Pergamon-Elsevier Science Ltd; Experimental Gerontology; 36; 2; 2-2001; 267-282
0531-5565
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/s0531-5565(00)00211-4
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0531556500002114
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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