Immunohistochemical distribution of early pregnancy factor in ovary, oviduct and placenta of pregnant gilts

Autores
Grosso, Maria Carolina; Bellingeri, Romina Valeria; Motta, C; Alustiza, Fabrisio Eduardo; Picco, Natalia Yanina; Vivas, Adriana Beatriz
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Early pregnancy factor (EPF) is an immunosuppressant that promotes maternal immune system tolerance of the allogenic fetus. Little is known about localization of this factor in different tissues and nothing has been reported about localization in swine reproductive and placental tissues. We determined the concentration of EPF in serum of gilts and porcine placenta conditioned medium (PPCM). We also analyzed the expression of EPF in different reproductive tissues of pregnant gilts at 10, 30, 60 and 90 days of pregnancy. EPF concentration in serum and PPCM was determined by western blot and densitometry. EPF expression in reproductive tissue was assessed by immunohistochemistry. The highest concentration of EPF was observed at 30 days in serum and PPCM; the concentration was higher in PPCM than in serum at the stages we evaluated. All reproductive tissues from the gestational stages analyzed showed specific labeling of EPF, but this labeling did not appear in non-pregnant gilts. At 30 days pregnancy, the EPF expression in the ovary was predominantly in follicular lutein cells, probably owing to its function as a luteotrophic factor. In the oviduct, EPF was expressed in unciliated secretory epithelial cells and in the cilia of ciliated cells. In the placenta, EPF was expressed in the fetal portion (mesoderm chorioallantois and epithelium of endoderm). EPF acts as an autocrine and paracrine growth factor for the trophoblast during the peri-implantation period.
Fil: Grosso, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; Argentina
Fil: Bellingeri, Romina Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; Argentina
Fil: Motta, C. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; Argentina
Fil: Alustiza, Fabrisio Eduardo. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; Argentina
Fil: Picco, Natalia Yanina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; Argentina
Fil: Vivas, Adriana Beatriz. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; Argentina
Materia
Early Pregnancy Factor
Immunolocalization
Ovary
Oviduct
Placenta
Swine
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/34094
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/34094
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Immunohistochemical distribution of early pregnancy factor in ovary, oviduct and placenta of pregnant giltsGrosso, Maria CarolinaBellingeri, Romina ValeriaMotta, CAlustiza, Fabrisio EduardoPicco, Natalia YaninaVivas, Adriana BeatrizEarly Pregnancy FactorImmunolocalizationOvaryOviductPlacentaSwinehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Early pregnancy factor (EPF) is an immunosuppressant that promotes maternal immune system tolerance of the allogenic fetus. Little is known about localization of this factor in different tissues and nothing has been reported about localization in swine reproductive and placental tissues. We determined the concentration of EPF in serum of gilts and porcine placenta conditioned medium (PPCM). We also analyzed the expression of EPF in different reproductive tissues of pregnant gilts at 10, 30, 60 and 90 days of pregnancy. EPF concentration in serum and PPCM was determined by western blot and densitometry. EPF expression in reproductive tissue was assessed by immunohistochemistry. The highest concentration of EPF was observed at 30 days in serum and PPCM; the concentration was higher in PPCM than in serum at the stages we evaluated. All reproductive tissues from the gestational stages analyzed showed specific labeling of EPF, but this labeling did not appear in non-pregnant gilts. At 30 days pregnancy, the EPF expression in the ovary was predominantly in follicular lutein cells, probably owing to its function as a luteotrophic factor. In the oviduct, EPF was expressed in unciliated secretory epithelial cells and in the cilia of ciliated cells. In the placenta, EPF was expressed in the fetal portion (mesoderm chorioallantois and epithelium of endoderm). EPF acts as an autocrine and paracrine growth factor for the trophoblast during the peri-implantation period.Fil: Grosso, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; ArgentinaFil: Bellingeri, Romina Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; ArgentinaFil: Motta, C. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; ArgentinaFil: Alustiza, Fabrisio Eduardo. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; ArgentinaFil: Picco, Natalia Yanina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; ArgentinaFil: Vivas, Adriana Beatriz. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; ArgentinaInforma Healthcare2014-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/34094Grosso, Maria Carolina; Bellingeri, Romina Valeria; Motta, C; Alustiza, Fabrisio Eduardo; Picco, Natalia Yanina; et al.; Immunohistochemical distribution of early pregnancy factor in ovary, oviduct and placenta of pregnant gilts; Informa Healthcare; Biotechnic And Histochemistry; 90; 1; 6-2014; 14-241052-0295CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3109/10520295.2014.931599info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/full/10.3109/10520295.2014.931599info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:33:32Zoai:ri.conicet.gov.ar:11336/34094instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:33:33.128CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Immunohistochemical distribution of early pregnancy factor in ovary, oviduct and placenta of pregnant gilts
title Immunohistochemical distribution of early pregnancy factor in ovary, oviduct and placenta of pregnant gilts
spellingShingle Immunohistochemical distribution of early pregnancy factor in ovary, oviduct and placenta of pregnant gilts
Grosso, Maria Carolina
Early Pregnancy Factor
Immunolocalization
Ovary
Oviduct
Placenta
Swine
title_short Immunohistochemical distribution of early pregnancy factor in ovary, oviduct and placenta of pregnant gilts
title_full Immunohistochemical distribution of early pregnancy factor in ovary, oviduct and placenta of pregnant gilts
title_fullStr Immunohistochemical distribution of early pregnancy factor in ovary, oviduct and placenta of pregnant gilts
title_full_unstemmed Immunohistochemical distribution of early pregnancy factor in ovary, oviduct and placenta of pregnant gilts
title_sort Immunohistochemical distribution of early pregnancy factor in ovary, oviduct and placenta of pregnant gilts
dc.creator.none.fl_str_mv Grosso, Maria Carolina
Bellingeri, Romina Valeria
Motta, C
Alustiza, Fabrisio Eduardo
Picco, Natalia Yanina
Vivas, Adriana Beatriz
author Grosso, Maria Carolina
author_facet Grosso, Maria Carolina
Bellingeri, Romina Valeria
Motta, C
Alustiza, Fabrisio Eduardo
Picco, Natalia Yanina
Vivas, Adriana Beatriz
author_role author
author2 Bellingeri, Romina Valeria
Motta, C
Alustiza, Fabrisio Eduardo
Picco, Natalia Yanina
Vivas, Adriana Beatriz
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Early Pregnancy Factor
Immunolocalization
Ovary
Oviduct
Placenta
Swine
topic Early Pregnancy Factor
Immunolocalization
Ovary
Oviduct
Placenta
Swine
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Early pregnancy factor (EPF) is an immunosuppressant that promotes maternal immune system tolerance of the allogenic fetus. Little is known about localization of this factor in different tissues and nothing has been reported about localization in swine reproductive and placental tissues. We determined the concentration of EPF in serum of gilts and porcine placenta conditioned medium (PPCM). We also analyzed the expression of EPF in different reproductive tissues of pregnant gilts at 10, 30, 60 and 90 days of pregnancy. EPF concentration in serum and PPCM was determined by western blot and densitometry. EPF expression in reproductive tissue was assessed by immunohistochemistry. The highest concentration of EPF was observed at 30 days in serum and PPCM; the concentration was higher in PPCM than in serum at the stages we evaluated. All reproductive tissues from the gestational stages analyzed showed specific labeling of EPF, but this labeling did not appear in non-pregnant gilts. At 30 days pregnancy, the EPF expression in the ovary was predominantly in follicular lutein cells, probably owing to its function as a luteotrophic factor. In the oviduct, EPF was expressed in unciliated secretory epithelial cells and in the cilia of ciliated cells. In the placenta, EPF was expressed in the fetal portion (mesoderm chorioallantois and epithelium of endoderm). EPF acts as an autocrine and paracrine growth factor for the trophoblast during the peri-implantation period.
Fil: Grosso, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; Argentina
Fil: Bellingeri, Romina Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; Argentina
Fil: Motta, C. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; Argentina
Fil: Alustiza, Fabrisio Eduardo. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; Argentina
Fil: Picco, Natalia Yanina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; Argentina
Fil: Vivas, Adriana Beatriz. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; Argentina
description Early pregnancy factor (EPF) is an immunosuppressant that promotes maternal immune system tolerance of the allogenic fetus. Little is known about localization of this factor in different tissues and nothing has been reported about localization in swine reproductive and placental tissues. We determined the concentration of EPF in serum of gilts and porcine placenta conditioned medium (PPCM). We also analyzed the expression of EPF in different reproductive tissues of pregnant gilts at 10, 30, 60 and 90 days of pregnancy. EPF concentration in serum and PPCM was determined by western blot and densitometry. EPF expression in reproductive tissue was assessed by immunohistochemistry. The highest concentration of EPF was observed at 30 days in serum and PPCM; the concentration was higher in PPCM than in serum at the stages we evaluated. All reproductive tissues from the gestational stages analyzed showed specific labeling of EPF, but this labeling did not appear in non-pregnant gilts. At 30 days pregnancy, the EPF expression in the ovary was predominantly in follicular lutein cells, probably owing to its function as a luteotrophic factor. In the oviduct, EPF was expressed in unciliated secretory epithelial cells and in the cilia of ciliated cells. In the placenta, EPF was expressed in the fetal portion (mesoderm chorioallantois and epithelium of endoderm). EPF acts as an autocrine and paracrine growth factor for the trophoblast during the peri-implantation period.
publishDate 2014
dc.date.none.fl_str_mv 2014-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/34094
Grosso, Maria Carolina; Bellingeri, Romina Valeria; Motta, C; Alustiza, Fabrisio Eduardo; Picco, Natalia Yanina; et al.; Immunohistochemical distribution of early pregnancy factor in ovary, oviduct and placenta of pregnant gilts; Informa Healthcare; Biotechnic And Histochemistry; 90; 1; 6-2014; 14-24
1052-0295
CONICET Digital
CONICET
url http://hdl.handle.net/11336/34094
identifier_str_mv Grosso, Maria Carolina; Bellingeri, Romina Valeria; Motta, C; Alustiza, Fabrisio Eduardo; Picco, Natalia Yanina; et al.; Immunohistochemical distribution of early pregnancy factor in ovary, oviduct and placenta of pregnant gilts; Informa Healthcare; Biotechnic And Histochemistry; 90; 1; 6-2014; 14-24
1052-0295
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3109/10520295.2014.931599
info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/full/10.3109/10520295.2014.931599
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Informa Healthcare
publisher.none.fl_str_mv Informa Healthcare
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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