Neutralization of early pregnancy factor by passive immunization alters normal embryonic development and cytokine balance

Autores
Grosso, Maria Carolina; Bellingeri, Romina Valeria; Schade, Rüdiger; Vivas, Adriana Beatriz
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this study was to evaluate the effects of passive immunization with polyclonal anti-swine early pregnancy factor (EPF) antibody in pregnant rats during the pre-implantation period, and to analyse the regional distribution of EPF in placenta of rats. The immunodetection of EPF in rat placenta, as well as fertilization, development and immunological parameters, was evaluated. The average number of embryos and the embryo/corpora lutea ratio of rats treated with anti-EPF antibody were significantly lower than control groups. Also, as expected, the embryos showed a delay in their development. Furthermore, while IL-10 and INF- levels increased, serum and placental TGF- decreased significantly in the group treated with anti-EPF. EPF was presentin giant and decidual cells, as well as in blood vessels and trophoblastic lacuna cells, as demonstrated by positive immunostaining. This study provides new results on the function and location of EPF, and has demonstrated the usefulness of polyclonal anti-swine EPF antibody.
Fil: Grosso, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Anatomía Animal; Argentina
Fil: Bellingeri, Romina Valeria. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Anatomía Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Schade, Rüdiger. Technishe Universitat Berlin; Alemania
Fil: Vivas, Adriana Beatriz. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Anatomía Animal; Argentina
Materia
EARLY PREGNANCY FACTOR
EARLY DEVELOPMENT
CYTOKINES
IMPLANTATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/269132
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/269132
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Neutralization of early pregnancy factor by passive immunization alters normal embryonic development and cytokine balanceGrosso, Maria CarolinaBellingeri, Romina ValeriaSchade, RüdigerVivas, Adriana BeatrizEARLY PREGNANCY FACTOREARLY DEVELOPMENTCYTOKINESIMPLANTATIONhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The aim of this study was to evaluate the effects of passive immunization with polyclonal anti-swine early pregnancy factor (EPF) antibody in pregnant rats during the pre-implantation period, and to analyse the regional distribution of EPF in placenta of rats. The immunodetection of EPF in rat placenta, as well as fertilization, development and immunological parameters, was evaluated. The average number of embryos and the embryo/corpora lutea ratio of rats treated with anti-EPF antibody were significantly lower than control groups. Also, as expected, the embryos showed a delay in their development. Furthermore, while IL-10 and INF- levels increased, serum and placental TGF- decreased significantly in the group treated with anti-EPF. EPF was presentin giant and decidual cells, as well as in blood vessels and trophoblastic lacuna cells, as demonstrated by positive immunostaining. This study provides new results on the function and location of EPF, and has demonstrated the usefulness of polyclonal anti-swine EPF antibody.Fil: Grosso, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Anatomía Animal; ArgentinaFil: Bellingeri, Romina Valeria. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Anatomía Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Schade, Rüdiger. Technishe Universitat Berlin; AlemaniaFil: Vivas, Adriana Beatriz. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Anatomía Animal; ArgentinaElsevier2012-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/269132Grosso, Maria Carolina; Bellingeri, Romina Valeria; Schade, Rüdiger; Vivas, Adriana Beatriz; Neutralization of early pregnancy factor by passive immunization alters normal embryonic development and cytokine balance; Elsevier; Inmunología; 31; 4; 10-2012; 106-1140213-9626CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0213962612000923info:eu-repo/semantics/altIdentifier/doi/10.1016/j.inmuno.2012.09.003info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:04:47Zoai:ri.conicet.gov.ar:11336/269132instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:04:48.185CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Neutralization of early pregnancy factor by passive immunization alters normal embryonic development and cytokine balance
title Neutralization of early pregnancy factor by passive immunization alters normal embryonic development and cytokine balance
spellingShingle Neutralization of early pregnancy factor by passive immunization alters normal embryonic development and cytokine balance
Grosso, Maria Carolina
EARLY PREGNANCY FACTOR
EARLY DEVELOPMENT
CYTOKINES
IMPLANTATION
title_short Neutralization of early pregnancy factor by passive immunization alters normal embryonic development and cytokine balance
title_full Neutralization of early pregnancy factor by passive immunization alters normal embryonic development and cytokine balance
title_fullStr Neutralization of early pregnancy factor by passive immunization alters normal embryonic development and cytokine balance
title_full_unstemmed Neutralization of early pregnancy factor by passive immunization alters normal embryonic development and cytokine balance
title_sort Neutralization of early pregnancy factor by passive immunization alters normal embryonic development and cytokine balance
dc.creator.none.fl_str_mv Grosso, Maria Carolina
Bellingeri, Romina Valeria
Schade, Rüdiger
Vivas, Adriana Beatriz
author Grosso, Maria Carolina
author_facet Grosso, Maria Carolina
Bellingeri, Romina Valeria
Schade, Rüdiger
Vivas, Adriana Beatriz
author_role author
author2 Bellingeri, Romina Valeria
Schade, Rüdiger
Vivas, Adriana Beatriz
author2_role author
author
author
dc.subject.none.fl_str_mv EARLY PREGNANCY FACTOR
EARLY DEVELOPMENT
CYTOKINES
IMPLANTATION
topic EARLY PREGNANCY FACTOR
EARLY DEVELOPMENT
CYTOKINES
IMPLANTATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The aim of this study was to evaluate the effects of passive immunization with polyclonal anti-swine early pregnancy factor (EPF) antibody in pregnant rats during the pre-implantation period, and to analyse the regional distribution of EPF in placenta of rats. The immunodetection of EPF in rat placenta, as well as fertilization, development and immunological parameters, was evaluated. The average number of embryos and the embryo/corpora lutea ratio of rats treated with anti-EPF antibody were significantly lower than control groups. Also, as expected, the embryos showed a delay in their development. Furthermore, while IL-10 and INF- levels increased, serum and placental TGF- decreased significantly in the group treated with anti-EPF. EPF was presentin giant and decidual cells, as well as in blood vessels and trophoblastic lacuna cells, as demonstrated by positive immunostaining. This study provides new results on the function and location of EPF, and has demonstrated the usefulness of polyclonal anti-swine EPF antibody.
Fil: Grosso, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Anatomía Animal; Argentina
Fil: Bellingeri, Romina Valeria. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Anatomía Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Schade, Rüdiger. Technishe Universitat Berlin; Alemania
Fil: Vivas, Adriana Beatriz. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Anatomía Animal; Argentina
description The aim of this study was to evaluate the effects of passive immunization with polyclonal anti-swine early pregnancy factor (EPF) antibody in pregnant rats during the pre-implantation period, and to analyse the regional distribution of EPF in placenta of rats. The immunodetection of EPF in rat placenta, as well as fertilization, development and immunological parameters, was evaluated. The average number of embryos and the embryo/corpora lutea ratio of rats treated with anti-EPF antibody were significantly lower than control groups. Also, as expected, the embryos showed a delay in their development. Furthermore, while IL-10 and INF- levels increased, serum and placental TGF- decreased significantly in the group treated with anti-EPF. EPF was presentin giant and decidual cells, as well as in blood vessels and trophoblastic lacuna cells, as demonstrated by positive immunostaining. This study provides new results on the function and location of EPF, and has demonstrated the usefulness of polyclonal anti-swine EPF antibody.
publishDate 2012
dc.date.none.fl_str_mv 2012-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/269132
Grosso, Maria Carolina; Bellingeri, Romina Valeria; Schade, Rüdiger; Vivas, Adriana Beatriz; Neutralization of early pregnancy factor by passive immunization alters normal embryonic development and cytokine balance; Elsevier; Inmunología; 31; 4; 10-2012; 106-114
0213-9626
CONICET Digital
CONICET
url http://hdl.handle.net/11336/269132
identifier_str_mv Grosso, Maria Carolina; Bellingeri, Romina Valeria; Schade, Rüdiger; Vivas, Adriana Beatriz; Neutralization of early pregnancy factor by passive immunization alters normal embryonic development and cytokine balance; Elsevier; Inmunología; 31; 4; 10-2012; 106-114
0213-9626
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0213962612000923
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.inmuno.2012.09.003
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083190990569472
score 13.22299

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