Omental adipose tissue is a more suitable source of canine Mesenchymal stem cells
- Autores
- Bahamondes, Francisca; Flores, Estefania; Cattaneo, Gino; Bruna, Flavia Alejandra; Conget, Paulette
- Año de publicación
- 2017
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Background: Mesenchymal Stem Cells (MSCs) are a promising therapeutic tool in veterinary medicine. Currently the subcutaneous adipose tissue is the leading source of MSCs in dogs. MSCs derived from distinct fat depots have shown dissimilarities in their accessibility and therapeutic potential. The aims of our work were to determine the suitability of omental adipose tissue as a source of MSCs, according to sampling success, cell yield and paracrine properties of isolated cells, and compared to subcutaneous adipose tissue. Results: While sampling success of omental adipose tissue was 100% (14 collections from14 donors) for subcutaneous adipose tissue it was 71% (10 collections from 14 donors). MSCs could be isolated from both sources. Cell yield was significantly higher for omental than for subcutaneous adipose tissue (38 ± 1 vs. 30 ± 1 CFU-F/g tissue, p < 0.0001). No differences were observed between sources regarding cell proliferation potential (73 ± 1 vs. 74 ± 1 CDPL) and cell senescence (at passage 10, both cultures presented enlarged cells with cytoplasmic vacuoles and cellular debris). Omental- and subcutaneous-derived MSCs expressed at the same level bFGF, PDGF, HGF, VEGF, ANG1 and IL-10. Irrespective of the source, isolated MSCs induced proliferation, migration and vascularization of target cells, and inhibited the activation of T lymphocytes. Conclusion: Compared to subcutaneous adipose tissue, omental adipose tissue is a more suitable source of MSCs in dogs. Since it can be procured from donors with any body condition, its collection procedure is always feasible, its cell yield is high and the MSCs isolated from it have desirable differentiation and paracrine potentials.
Fil: Bahamondes, Francisca. Universidad de Chile; Chile. Universidad del Desarrollo; Chile
Fil: Flores, Estefania. Universidad de Chile; Chile
Fil: Cattaneo, Gino. Universidad de Chile; Chile
Fil: Bruna, Flavia Alejandra. Universidad del Desarrollo; Chile. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Conget, Paulette. Universidad del Desarrollo; Chile - Materia
-
ADIPOSE TISSUE
CANINE
DOG
MESENCHYMAL STEM CELL
OMENTUM
SOURCE - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/50191
Ver los metadatos del registro completo
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Omental adipose tissue is a more suitable source of canine Mesenchymal stem cellsBahamondes, FranciscaFlores, EstefaniaCattaneo, GinoBruna, Flavia AlejandraConget, PauletteADIPOSE TISSUECANINEDOGMESENCHYMAL STEM CELLOMENTUMSOURCEhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Background: Mesenchymal Stem Cells (MSCs) are a promising therapeutic tool in veterinary medicine. Currently the subcutaneous adipose tissue is the leading source of MSCs in dogs. MSCs derived from distinct fat depots have shown dissimilarities in their accessibility and therapeutic potential. The aims of our work were to determine the suitability of omental adipose tissue as a source of MSCs, according to sampling success, cell yield and paracrine properties of isolated cells, and compared to subcutaneous adipose tissue. Results: While sampling success of omental adipose tissue was 100% (14 collections from14 donors) for subcutaneous adipose tissue it was 71% (10 collections from 14 donors). MSCs could be isolated from both sources. Cell yield was significantly higher for omental than for subcutaneous adipose tissue (38 ± 1 vs. 30 ± 1 CFU-F/g tissue, p < 0.0001). No differences were observed between sources regarding cell proliferation potential (73 ± 1 vs. 74 ± 1 CDPL) and cell senescence (at passage 10, both cultures presented enlarged cells with cytoplasmic vacuoles and cellular debris). Omental- and subcutaneous-derived MSCs expressed at the same level bFGF, PDGF, HGF, VEGF, ANG1 and IL-10. Irrespective of the source, isolated MSCs induced proliferation, migration and vascularization of target cells, and inhibited the activation of T lymphocytes. Conclusion: Compared to subcutaneous adipose tissue, omental adipose tissue is a more suitable source of MSCs in dogs. Since it can be procured from donors with any body condition, its collection procedure is always feasible, its cell yield is high and the MSCs isolated from it have desirable differentiation and paracrine potentials.Fil: Bahamondes, Francisca. Universidad de Chile; Chile. Universidad del Desarrollo; ChileFil: Flores, Estefania. Universidad de Chile; ChileFil: Cattaneo, Gino. Universidad de Chile; ChileFil: Bruna, Flavia Alejandra. Universidad del Desarrollo; Chile. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Conget, Paulette. Universidad del Desarrollo; ChileBioMed Central2017-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/50191Bahamondes, Francisca; Flores, Estefania; Cattaneo, Gino; Bruna, Flavia Alejandra; Conget, Paulette; Omental adipose tissue is a more suitable source of canine Mesenchymal stem cells; BioMed Central; BMC Veterinary Research; 13; 1; 6-2017; 1-91746-6148CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1186/s12917-017-1053-0info:eu-repo/semantics/altIdentifier/url/https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-017-1053-0info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:36:45Zoai:ri.conicet.gov.ar:11336/50191instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:36:46.051CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Omental adipose tissue is a more suitable source of canine Mesenchymal stem cells |
title |
Omental adipose tissue is a more suitable source of canine Mesenchymal stem cells |
spellingShingle |
Omental adipose tissue is a more suitable source of canine Mesenchymal stem cells Bahamondes, Francisca ADIPOSE TISSUE CANINE DOG MESENCHYMAL STEM CELL OMENTUM SOURCE |
title_short |
Omental adipose tissue is a more suitable source of canine Mesenchymal stem cells |
title_full |
Omental adipose tissue is a more suitable source of canine Mesenchymal stem cells |
title_fullStr |
Omental adipose tissue is a more suitable source of canine Mesenchymal stem cells |
title_full_unstemmed |
Omental adipose tissue is a more suitable source of canine Mesenchymal stem cells |
title_sort |
Omental adipose tissue is a more suitable source of canine Mesenchymal stem cells |
dc.creator.none.fl_str_mv |
Bahamondes, Francisca Flores, Estefania Cattaneo, Gino Bruna, Flavia Alejandra Conget, Paulette |
author |
Bahamondes, Francisca |
author_facet |
Bahamondes, Francisca Flores, Estefania Cattaneo, Gino Bruna, Flavia Alejandra Conget, Paulette |
author_role |
author |
author2 |
Flores, Estefania Cattaneo, Gino Bruna, Flavia Alejandra Conget, Paulette |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
ADIPOSE TISSUE CANINE DOG MESENCHYMAL STEM CELL OMENTUM SOURCE |
topic |
ADIPOSE TISSUE CANINE DOG MESENCHYMAL STEM CELL OMENTUM SOURCE |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/4.3 https://purl.org/becyt/ford/4 |
dc.description.none.fl_txt_mv |
Background: Mesenchymal Stem Cells (MSCs) are a promising therapeutic tool in veterinary medicine. Currently the subcutaneous adipose tissue is the leading source of MSCs in dogs. MSCs derived from distinct fat depots have shown dissimilarities in their accessibility and therapeutic potential. The aims of our work were to determine the suitability of omental adipose tissue as a source of MSCs, according to sampling success, cell yield and paracrine properties of isolated cells, and compared to subcutaneous adipose tissue. Results: While sampling success of omental adipose tissue was 100% (14 collections from14 donors) for subcutaneous adipose tissue it was 71% (10 collections from 14 donors). MSCs could be isolated from both sources. Cell yield was significantly higher for omental than for subcutaneous adipose tissue (38 ± 1 vs. 30 ± 1 CFU-F/g tissue, p < 0.0001). No differences were observed between sources regarding cell proliferation potential (73 ± 1 vs. 74 ± 1 CDPL) and cell senescence (at passage 10, both cultures presented enlarged cells with cytoplasmic vacuoles and cellular debris). Omental- and subcutaneous-derived MSCs expressed at the same level bFGF, PDGF, HGF, VEGF, ANG1 and IL-10. Irrespective of the source, isolated MSCs induced proliferation, migration and vascularization of target cells, and inhibited the activation of T lymphocytes. Conclusion: Compared to subcutaneous adipose tissue, omental adipose tissue is a more suitable source of MSCs in dogs. Since it can be procured from donors with any body condition, its collection procedure is always feasible, its cell yield is high and the MSCs isolated from it have desirable differentiation and paracrine potentials. Fil: Bahamondes, Francisca. Universidad de Chile; Chile. Universidad del Desarrollo; Chile Fil: Flores, Estefania. Universidad de Chile; Chile Fil: Cattaneo, Gino. Universidad de Chile; Chile Fil: Bruna, Flavia Alejandra. Universidad del Desarrollo; Chile. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina Fil: Conget, Paulette. Universidad del Desarrollo; Chile |
description |
Background: Mesenchymal Stem Cells (MSCs) are a promising therapeutic tool in veterinary medicine. Currently the subcutaneous adipose tissue is the leading source of MSCs in dogs. MSCs derived from distinct fat depots have shown dissimilarities in their accessibility and therapeutic potential. The aims of our work were to determine the suitability of omental adipose tissue as a source of MSCs, according to sampling success, cell yield and paracrine properties of isolated cells, and compared to subcutaneous adipose tissue. Results: While sampling success of omental adipose tissue was 100% (14 collections from14 donors) for subcutaneous adipose tissue it was 71% (10 collections from 14 donors). MSCs could be isolated from both sources. Cell yield was significantly higher for omental than for subcutaneous adipose tissue (38 ± 1 vs. 30 ± 1 CFU-F/g tissue, p < 0.0001). No differences were observed between sources regarding cell proliferation potential (73 ± 1 vs. 74 ± 1 CDPL) and cell senescence (at passage 10, both cultures presented enlarged cells with cytoplasmic vacuoles and cellular debris). Omental- and subcutaneous-derived MSCs expressed at the same level bFGF, PDGF, HGF, VEGF, ANG1 and IL-10. Irrespective of the source, isolated MSCs induced proliferation, migration and vascularization of target cells, and inhibited the activation of T lymphocytes. Conclusion: Compared to subcutaneous adipose tissue, omental adipose tissue is a more suitable source of MSCs in dogs. Since it can be procured from donors with any body condition, its collection procedure is always feasible, its cell yield is high and the MSCs isolated from it have desirable differentiation and paracrine potentials. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-06 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/50191 Bahamondes, Francisca; Flores, Estefania; Cattaneo, Gino; Bruna, Flavia Alejandra; Conget, Paulette; Omental adipose tissue is a more suitable source of canine Mesenchymal stem cells; BioMed Central; BMC Veterinary Research; 13; 1; 6-2017; 1-9 1746-6148 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/50191 |
identifier_str_mv |
Bahamondes, Francisca; Flores, Estefania; Cattaneo, Gino; Bruna, Flavia Alejandra; Conget, Paulette; Omental adipose tissue is a more suitable source of canine Mesenchymal stem cells; BioMed Central; BMC Veterinary Research; 13; 1; 6-2017; 1-9 1746-6148 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1186/s12917-017-1053-0 info:eu-repo/semantics/altIdentifier/url/https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-017-1053-0 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
BioMed Central |
publisher.none.fl_str_mv |
BioMed Central |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613154712059904 |
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13.070432 |