Omental adipose tissue is a more suitable source of canine Mesenchymal stem cells

Autores
Bahamondes, Francisca; Flores, Estefania; Cattaneo, Gino; Bruna, Flavia Alejandra; Conget, Paulette
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Mesenchymal Stem Cells (MSCs) are a promising therapeutic tool in veterinary medicine. Currently the subcutaneous adipose tissue is the leading source of MSCs in dogs. MSCs derived from distinct fat depots have shown dissimilarities in their accessibility and therapeutic potential. The aims of our work were to determine the suitability of omental adipose tissue as a source of MSCs, according to sampling success, cell yield and paracrine properties of isolated cells, and compared to subcutaneous adipose tissue. Results: While sampling success of omental adipose tissue was 100% (14 collections from14 donors) for subcutaneous adipose tissue it was 71% (10 collections from 14 donors). MSCs could be isolated from both sources. Cell yield was significantly higher for omental than for subcutaneous adipose tissue (38 ± 1 vs. 30 ± 1 CFU-F/g tissue, p < 0.0001). No differences were observed between sources regarding cell proliferation potential (73 ± 1 vs. 74 ± 1 CDPL) and cell senescence (at passage 10, both cultures presented enlarged cells with cytoplasmic vacuoles and cellular debris). Omental- and subcutaneous-derived MSCs expressed at the same level bFGF, PDGF, HGF, VEGF, ANG1 and IL-10. Irrespective of the source, isolated MSCs induced proliferation, migration and vascularization of target cells, and inhibited the activation of T lymphocytes. Conclusion: Compared to subcutaneous adipose tissue, omental adipose tissue is a more suitable source of MSCs in dogs. Since it can be procured from donors with any body condition, its collection procedure is always feasible, its cell yield is high and the MSCs isolated from it have desirable differentiation and paracrine potentials.
Fil: Bahamondes, Francisca. Universidad de Chile; Chile. Universidad del Desarrollo; Chile
Fil: Flores, Estefania. Universidad de Chile; Chile
Fil: Cattaneo, Gino. Universidad de Chile; Chile
Fil: Bruna, Flavia Alejandra. Universidad del Desarrollo; Chile. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Conget, Paulette. Universidad del Desarrollo; Chile
Materia
ADIPOSE TISSUE
CANINE
DOG
MESENCHYMAL STEM CELL
OMENTUM
SOURCE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/50191
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/50191
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Omental adipose tissue is a more suitable source of canine Mesenchymal stem cellsBahamondes, FranciscaFlores, EstefaniaCattaneo, GinoBruna, Flavia AlejandraConget, PauletteADIPOSE TISSUECANINEDOGMESENCHYMAL STEM CELLOMENTUMSOURCEhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Background: Mesenchymal Stem Cells (MSCs) are a promising therapeutic tool in veterinary medicine. Currently the subcutaneous adipose tissue is the leading source of MSCs in dogs. MSCs derived from distinct fat depots have shown dissimilarities in their accessibility and therapeutic potential. The aims of our work were to determine the suitability of omental adipose tissue as a source of MSCs, according to sampling success, cell yield and paracrine properties of isolated cells, and compared to subcutaneous adipose tissue. Results: While sampling success of omental adipose tissue was 100% (14 collections from14 donors) for subcutaneous adipose tissue it was 71% (10 collections from 14 donors). MSCs could be isolated from both sources. Cell yield was significantly higher for omental than for subcutaneous adipose tissue (38 ± 1 vs. 30 ± 1 CFU-F/g tissue, p < 0.0001). No differences were observed between sources regarding cell proliferation potential (73 ± 1 vs. 74 ± 1 CDPL) and cell senescence (at passage 10, both cultures presented enlarged cells with cytoplasmic vacuoles and cellular debris). Omental- and subcutaneous-derived MSCs expressed at the same level bFGF, PDGF, HGF, VEGF, ANG1 and IL-10. Irrespective of the source, isolated MSCs induced proliferation, migration and vascularization of target cells, and inhibited the activation of T lymphocytes. Conclusion: Compared to subcutaneous adipose tissue, omental adipose tissue is a more suitable source of MSCs in dogs. Since it can be procured from donors with any body condition, its collection procedure is always feasible, its cell yield is high and the MSCs isolated from it have desirable differentiation and paracrine potentials.Fil: Bahamondes, Francisca. Universidad de Chile; Chile. Universidad del Desarrollo; ChileFil: Flores, Estefania. Universidad de Chile; ChileFil: Cattaneo, Gino. Universidad de Chile; ChileFil: Bruna, Flavia Alejandra. Universidad del Desarrollo; Chile. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Conget, Paulette. Universidad del Desarrollo; ChileBioMed Central2017-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/50191Bahamondes, Francisca; Flores, Estefania; Cattaneo, Gino; Bruna, Flavia Alejandra; Conget, Paulette; Omental adipose tissue is a more suitable source of canine Mesenchymal stem cells; BioMed Central; BMC Veterinary Research; 13; 1; 6-2017; 1-91746-6148CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1186/s12917-017-1053-0info:eu-repo/semantics/altIdentifier/url/https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-017-1053-0info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:04:51Zoai:ri.conicet.gov.ar:11336/50191instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:04:52.295CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Omental adipose tissue is a more suitable source of canine Mesenchymal stem cells
title Omental adipose tissue is a more suitable source of canine Mesenchymal stem cells
spellingShingle Omental adipose tissue is a more suitable source of canine Mesenchymal stem cells
Bahamondes, Francisca
ADIPOSE TISSUE
CANINE
DOG
MESENCHYMAL STEM CELL
OMENTUM
SOURCE
title_short Omental adipose tissue is a more suitable source of canine Mesenchymal stem cells
title_full Omental adipose tissue is a more suitable source of canine Mesenchymal stem cells
title_fullStr Omental adipose tissue is a more suitable source of canine Mesenchymal stem cells
title_full_unstemmed Omental adipose tissue is a more suitable source of canine Mesenchymal stem cells
title_sort Omental adipose tissue is a more suitable source of canine Mesenchymal stem cells
dc.creator.none.fl_str_mv Bahamondes, Francisca
Flores, Estefania
Cattaneo, Gino
Bruna, Flavia Alejandra
Conget, Paulette
author Bahamondes, Francisca
author_facet Bahamondes, Francisca
Flores, Estefania
Cattaneo, Gino
Bruna, Flavia Alejandra
Conget, Paulette
author_role author
author2 Flores, Estefania
Cattaneo, Gino
Bruna, Flavia Alejandra
Conget, Paulette
author2_role author
author
author
author
dc.subject.none.fl_str_mv ADIPOSE TISSUE
CANINE
DOG
MESENCHYMAL STEM CELL
OMENTUM
SOURCE
topic ADIPOSE TISSUE
CANINE
DOG
MESENCHYMAL STEM CELL
OMENTUM
SOURCE
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Background: Mesenchymal Stem Cells (MSCs) are a promising therapeutic tool in veterinary medicine. Currently the subcutaneous adipose tissue is the leading source of MSCs in dogs. MSCs derived from distinct fat depots have shown dissimilarities in their accessibility and therapeutic potential. The aims of our work were to determine the suitability of omental adipose tissue as a source of MSCs, according to sampling success, cell yield and paracrine properties of isolated cells, and compared to subcutaneous adipose tissue. Results: While sampling success of omental adipose tissue was 100% (14 collections from14 donors) for subcutaneous adipose tissue it was 71% (10 collections from 14 donors). MSCs could be isolated from both sources. Cell yield was significantly higher for omental than for subcutaneous adipose tissue (38 ± 1 vs. 30 ± 1 CFU-F/g tissue, p < 0.0001). No differences were observed between sources regarding cell proliferation potential (73 ± 1 vs. 74 ± 1 CDPL) and cell senescence (at passage 10, both cultures presented enlarged cells with cytoplasmic vacuoles and cellular debris). Omental- and subcutaneous-derived MSCs expressed at the same level bFGF, PDGF, HGF, VEGF, ANG1 and IL-10. Irrespective of the source, isolated MSCs induced proliferation, migration and vascularization of target cells, and inhibited the activation of T lymphocytes. Conclusion: Compared to subcutaneous adipose tissue, omental adipose tissue is a more suitable source of MSCs in dogs. Since it can be procured from donors with any body condition, its collection procedure is always feasible, its cell yield is high and the MSCs isolated from it have desirable differentiation and paracrine potentials.
Fil: Bahamondes, Francisca. Universidad de Chile; Chile. Universidad del Desarrollo; Chile
Fil: Flores, Estefania. Universidad de Chile; Chile
Fil: Cattaneo, Gino. Universidad de Chile; Chile
Fil: Bruna, Flavia Alejandra. Universidad del Desarrollo; Chile. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Conget, Paulette. Universidad del Desarrollo; Chile
description Background: Mesenchymal Stem Cells (MSCs) are a promising therapeutic tool in veterinary medicine. Currently the subcutaneous adipose tissue is the leading source of MSCs in dogs. MSCs derived from distinct fat depots have shown dissimilarities in their accessibility and therapeutic potential. The aims of our work were to determine the suitability of omental adipose tissue as a source of MSCs, according to sampling success, cell yield and paracrine properties of isolated cells, and compared to subcutaneous adipose tissue. Results: While sampling success of omental adipose tissue was 100% (14 collections from14 donors) for subcutaneous adipose tissue it was 71% (10 collections from 14 donors). MSCs could be isolated from both sources. Cell yield was significantly higher for omental than for subcutaneous adipose tissue (38 ± 1 vs. 30 ± 1 CFU-F/g tissue, p < 0.0001). No differences were observed between sources regarding cell proliferation potential (73 ± 1 vs. 74 ± 1 CDPL) and cell senescence (at passage 10, both cultures presented enlarged cells with cytoplasmic vacuoles and cellular debris). Omental- and subcutaneous-derived MSCs expressed at the same level bFGF, PDGF, HGF, VEGF, ANG1 and IL-10. Irrespective of the source, isolated MSCs induced proliferation, migration and vascularization of target cells, and inhibited the activation of T lymphocytes. Conclusion: Compared to subcutaneous adipose tissue, omental adipose tissue is a more suitable source of MSCs in dogs. Since it can be procured from donors with any body condition, its collection procedure is always feasible, its cell yield is high and the MSCs isolated from it have desirable differentiation and paracrine potentials.
publishDate 2017
dc.date.none.fl_str_mv 2017-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/50191
Bahamondes, Francisca; Flores, Estefania; Cattaneo, Gino; Bruna, Flavia Alejandra; Conget, Paulette; Omental adipose tissue is a more suitable source of canine Mesenchymal stem cells; BioMed Central; BMC Veterinary Research; 13; 1; 6-2017; 1-9
1746-6148
CONICET Digital
CONICET
url http://hdl.handle.net/11336/50191
identifier_str_mv Bahamondes, Francisca; Flores, Estefania; Cattaneo, Gino; Bruna, Flavia Alejandra; Conget, Paulette; Omental adipose tissue is a more suitable source of canine Mesenchymal stem cells; BioMed Central; BMC Veterinary Research; 13; 1; 6-2017; 1-9
1746-6148
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1186/s12917-017-1053-0
info:eu-repo/semantics/altIdentifier/url/https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-017-1053-0
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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