Modulation of adipose-derived mesenchymal stem/stromal cell transcriptome by G-CSF stimulation

Autores
Avila Portillo, Luz M.; Aristizabal, Fabio; Riveros, Angela; Abba, Martín Carlos; Correa, Diego
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Mesenchymal stem/stromal cells (MSCs) exhibit multidifferentiation potential, paralleled with immunomodulatory and trophic properties that make them viable alternative tools for the treatment of degenerative disorders, allograft rejection, autoimmune diseases, and tissue regeneration. MSC functional attributes can be modulated by exposing them to inflammatory-stimulating microenvironments (i.e., priming) before their therapeutic use. Granulocyte-colony stimulating factor (G-CSF) is a cytokine that plays key roles in immune response and hematopoiesis modulation through direct effects on hematopoietic progenitors' proliferation, survival, and mobilization. Despite the established roles of MSCs supporting hematopoiesis, the effects of G-CSF on MSCs biology have not been thoroughly explored. This study reveals that G-CSF has also direct effects on adipose-derived MSCs (ADSCs), modulating their functions. Herein, microarray-based transcriptomic analysis shows that G-CSF stimulation in vitro results in modulation of various signaling pathways including ones related with the metabolism of hyaluronan (HA), conferring a profile of cell mobilization to ADSCs, mediated in a cell-intrinsic fashion in part by reducing CD44 expression and HA synthesis-related genes. Collectively, these data suggest a direct modulatory effect of G-CSF on ADSC function, potentially altering their therapeutic capacity and thus the design of future clinical protocols.
Fil: Avila Portillo, Luz M.. Universidad Nacional de Colombia; Colombia
Fil: Aristizabal, Fabio. Universidad Nacional de Colombia; Colombia
Fil: Riveros, Angela. No especifíca;
Fil: Abba, Martín Carlos. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Inmunológicas Básicas y Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Correa, Diego. University of Miami; Estados Unidos
Materia
G-CSF
Mesenchymal
STEM
CELLS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/131003
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/131003
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Modulation of adipose-derived mesenchymal stem/stromal cell transcriptome by G-CSF stimulationAvila Portillo, Luz M.Aristizabal, FabioRiveros, AngelaAbba, Martín CarlosCorrea, DiegoG-CSFMesenchymalSTEMCELLShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Mesenchymal stem/stromal cells (MSCs) exhibit multidifferentiation potential, paralleled with immunomodulatory and trophic properties that make them viable alternative tools for the treatment of degenerative disorders, allograft rejection, autoimmune diseases, and tissue regeneration. MSC functional attributes can be modulated by exposing them to inflammatory-stimulating microenvironments (i.e., priming) before their therapeutic use. Granulocyte-colony stimulating factor (G-CSF) is a cytokine that plays key roles in immune response and hematopoiesis modulation through direct effects on hematopoietic progenitors' proliferation, survival, and mobilization. Despite the established roles of MSCs supporting hematopoiesis, the effects of G-CSF on MSCs biology have not been thoroughly explored. This study reveals that G-CSF has also direct effects on adipose-derived MSCs (ADSCs), modulating their functions. Herein, microarray-based transcriptomic analysis shows that G-CSF stimulation in vitro results in modulation of various signaling pathways including ones related with the metabolism of hyaluronan (HA), conferring a profile of cell mobilization to ADSCs, mediated in a cell-intrinsic fashion in part by reducing CD44 expression and HA synthesis-related genes. Collectively, these data suggest a direct modulatory effect of G-CSF on ADSC function, potentially altering their therapeutic capacity and thus the design of future clinical protocols.Fil: Avila Portillo, Luz M.. Universidad Nacional de Colombia; ColombiaFil: Aristizabal, Fabio. Universidad Nacional de Colombia; ColombiaFil: Riveros, Angela. No especifíca;Fil: Abba, Martín Carlos. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Inmunológicas Básicas y Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Correa, Diego. University of Miami; Estados UnidosHindawi Publishing Corporation2020-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/131003Avila Portillo, Luz M.; Aristizabal, Fabio; Riveros, Angela; Abba, Martín Carlos; Correa, Diego; Modulation of adipose-derived mesenchymal stem/stromal cell transcriptome by G-CSF stimulation; Hindawi Publishing Corporation; Stem Cells International; 2020; 1-2020; 1-91687-9678CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1155/2020/5045124info:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/sci/2020/5045124/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:53:20Zoai:ri.conicet.gov.ar:11336/131003instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:53:21.216CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Modulation of adipose-derived mesenchymal stem/stromal cell transcriptome by G-CSF stimulation
title Modulation of adipose-derived mesenchymal stem/stromal cell transcriptome by G-CSF stimulation
spellingShingle Modulation of adipose-derived mesenchymal stem/stromal cell transcriptome by G-CSF stimulation
Avila Portillo, Luz M.
G-CSF
Mesenchymal
STEM
CELLS
title_short Modulation of adipose-derived mesenchymal stem/stromal cell transcriptome by G-CSF stimulation
title_full Modulation of adipose-derived mesenchymal stem/stromal cell transcriptome by G-CSF stimulation
title_fullStr Modulation of adipose-derived mesenchymal stem/stromal cell transcriptome by G-CSF stimulation
title_full_unstemmed Modulation of adipose-derived mesenchymal stem/stromal cell transcriptome by G-CSF stimulation
title_sort Modulation of adipose-derived mesenchymal stem/stromal cell transcriptome by G-CSF stimulation
dc.creator.none.fl_str_mv Avila Portillo, Luz M.
Aristizabal, Fabio
Riveros, Angela
Abba, Martín Carlos
Correa, Diego
author Avila Portillo, Luz M.
author_facet Avila Portillo, Luz M.
Aristizabal, Fabio
Riveros, Angela
Abba, Martín Carlos
Correa, Diego
author_role author
author2 Aristizabal, Fabio
Riveros, Angela
Abba, Martín Carlos
Correa, Diego
author2_role author
author
author
author
dc.subject.none.fl_str_mv G-CSF
Mesenchymal
STEM
CELLS
topic G-CSF
Mesenchymal
STEM
CELLS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Mesenchymal stem/stromal cells (MSCs) exhibit multidifferentiation potential, paralleled with immunomodulatory and trophic properties that make them viable alternative tools for the treatment of degenerative disorders, allograft rejection, autoimmune diseases, and tissue regeneration. MSC functional attributes can be modulated by exposing them to inflammatory-stimulating microenvironments (i.e., priming) before their therapeutic use. Granulocyte-colony stimulating factor (G-CSF) is a cytokine that plays key roles in immune response and hematopoiesis modulation through direct effects on hematopoietic progenitors' proliferation, survival, and mobilization. Despite the established roles of MSCs supporting hematopoiesis, the effects of G-CSF on MSCs biology have not been thoroughly explored. This study reveals that G-CSF has also direct effects on adipose-derived MSCs (ADSCs), modulating their functions. Herein, microarray-based transcriptomic analysis shows that G-CSF stimulation in vitro results in modulation of various signaling pathways including ones related with the metabolism of hyaluronan (HA), conferring a profile of cell mobilization to ADSCs, mediated in a cell-intrinsic fashion in part by reducing CD44 expression and HA synthesis-related genes. Collectively, these data suggest a direct modulatory effect of G-CSF on ADSC function, potentially altering their therapeutic capacity and thus the design of future clinical protocols.
Fil: Avila Portillo, Luz M.. Universidad Nacional de Colombia; Colombia
Fil: Aristizabal, Fabio. Universidad Nacional de Colombia; Colombia
Fil: Riveros, Angela. No especifíca;
Fil: Abba, Martín Carlos. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Inmunológicas Básicas y Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Correa, Diego. University of Miami; Estados Unidos
description Mesenchymal stem/stromal cells (MSCs) exhibit multidifferentiation potential, paralleled with immunomodulatory and trophic properties that make them viable alternative tools for the treatment of degenerative disorders, allograft rejection, autoimmune diseases, and tissue regeneration. MSC functional attributes can be modulated by exposing them to inflammatory-stimulating microenvironments (i.e., priming) before their therapeutic use. Granulocyte-colony stimulating factor (G-CSF) is a cytokine that plays key roles in immune response and hematopoiesis modulation through direct effects on hematopoietic progenitors' proliferation, survival, and mobilization. Despite the established roles of MSCs supporting hematopoiesis, the effects of G-CSF on MSCs biology have not been thoroughly explored. This study reveals that G-CSF has also direct effects on adipose-derived MSCs (ADSCs), modulating their functions. Herein, microarray-based transcriptomic analysis shows that G-CSF stimulation in vitro results in modulation of various signaling pathways including ones related with the metabolism of hyaluronan (HA), conferring a profile of cell mobilization to ADSCs, mediated in a cell-intrinsic fashion in part by reducing CD44 expression and HA synthesis-related genes. Collectively, these data suggest a direct modulatory effect of G-CSF on ADSC function, potentially altering their therapeutic capacity and thus the design of future clinical protocols.
publishDate 2020
dc.date.none.fl_str_mv 2020-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/131003
Avila Portillo, Luz M.; Aristizabal, Fabio; Riveros, Angela; Abba, Martín Carlos; Correa, Diego; Modulation of adipose-derived mesenchymal stem/stromal cell transcriptome by G-CSF stimulation; Hindawi Publishing Corporation; Stem Cells International; 2020; 1-2020; 1-9
1687-9678
CONICET Digital
CONICET
url http://hdl.handle.net/11336/131003
identifier_str_mv Avila Portillo, Luz M.; Aristizabal, Fabio; Riveros, Angela; Abba, Martín Carlos; Correa, Diego; Modulation of adipose-derived mesenchymal stem/stromal cell transcriptome by G-CSF stimulation; Hindawi Publishing Corporation; Stem Cells International; 2020; 1-2020; 1-9
1687-9678
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1155/2020/5045124
info:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/sci/2020/5045124/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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