Concanavalin-A induces granulosa cell death and inhibits fsh-mediated follicular growth and ovarian maturation in female rats

Autores
Velasquez, Ethel V.; Rios, Mariana; Ortiz, María Elena; Lizama, Carlos; Nuñez, Elizabeth; Abramovich, Dalhia Nurit; Orge, Felipe; Oliva, Bárbara; Orellana, Renán; Villalon, Manuel; Moreno, Ricardo D.; Tesone, Marta; Rokka, Anne; Corthals, Garry; Croxatto, Horacio B.; Parborell, Maria Fernanda Agustina; Owen, Gareth I.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Reproductive success stems from a finely regulated balance between follicular maturation and atresia, in which the role of carbohydrate structure is poorly understood. Here, we describe for the first time a fraction of purified recombinant human FSH that is capable of bringing about the cell death of granulosa cells and preventing follicular maturation in a rat model. Further analysis by mass spectrometry revealed the presence of the lectin Concanavalin-A (Con-A) within this fraction of recombinant FSH. Using both the fractionated FSH and Con-A, the observed cell death was predominantly located to the granulosa cells. Ex vivo culture of rat follicles demonstrated that follicle degeneration occurred and resulted in the release of a denuded and deteriorated oocyte. Moreover, in vivo experiments confirmed an increase in atresia and a corresponding reduction confined to follicle in early antral stage. As a mechanism of action, Con-A reduces ovarian proliferation, Von Willebrand staining, and angiogenesis. Based on the observation that Con-A may induce granulosa cell death followed by follicle death, our results further demonstrate that follicular carbohydrate moiety is changing under the influence of FSH, which may allow a carbohydrate- binding lectin to increase granulosa cell death. The physiological consequences of circulating lectin-like molecules remain to be determined. However, our results suggest a potential exploitation of carbohydrate binding in fertility and ovarian cancer treatment. This work may shed light on a key role of carbohydrates in the still obscure physiological process of follicular selection and atresia.
Fil: Velasquez, Ethel V.. Pontificia Universidad Católica de Chile; Chile
Fil: Rios, Mariana. Pontificia Universidad Católica de Chile; Chile
Fil: Ortiz, María Elena. Pontificia Universidad Católica de Chile; Chile
Fil: Lizama, Carlos. Pontificia Universidad Católica de Chile; Chile
Fil: Nuñez, Elizabeth. Pontificia Universidad Católica de Chile; Chile
Fil: Abramovich, Dalhia Nurit. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Orge, Felipe. Pontificia Universidad Católica de Chile; Chile
Fil: Oliva, Bárbara. Pontificia Universidad Católica de Chile; Chile
Fil: Orellana, Renán. Pontificia Universidad Católica de Chile; Chile
Fil: Villalon, Manuel. Pontificia Universidad Católica de Chile; Chile
Fil: Moreno, Ricardo D.. Pontificia Universidad Católica de Chile; Chile
Fil: Tesone, Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Rokka, Anne. Universidad Andrés Bello; Chile
Fil: Corthals, Garry. Universidad Andrés Bello; Chile
Fil: Croxatto, Horacio B.. Universidad de Santiago de Chile; Chile
Fil: Parborell, Maria Fernanda Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Owen, Gareth I.. Pontificia Universidad Católica de Chile; Chile
Materia
Concanavalin A
Granulosa Cells
Apoptosis
Fsh
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/2438
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/2438
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Concanavalin-A induces granulosa cell death and inhibits fsh-mediated follicular growth and ovarian maturation in female ratsVelasquez, Ethel V.Rios, MarianaOrtiz, María ElenaLizama, CarlosNuñez, ElizabethAbramovich, Dalhia NuritOrge, FelipeOliva, BárbaraOrellana, RenánVillalon, ManuelMoreno, Ricardo D.Tesone, MartaRokka, AnneCorthals, GarryCroxatto, Horacio B.Parborell, Maria Fernanda AgustinaOwen, Gareth I.Concanavalin AGranulosa CellsApoptosisFshhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Reproductive success stems from a finely regulated balance between follicular maturation and atresia, in which the role of carbohydrate structure is poorly understood. Here, we describe for the first time a fraction of purified recombinant human FSH that is capable of bringing about the cell death of granulosa cells and preventing follicular maturation in a rat model. Further analysis by mass spectrometry revealed the presence of the lectin Concanavalin-A (Con-A) within this fraction of recombinant FSH. Using both the fractionated FSH and Con-A, the observed cell death was predominantly located to the granulosa cells. Ex vivo culture of rat follicles demonstrated that follicle degeneration occurred and resulted in the release of a denuded and deteriorated oocyte. Moreover, in vivo experiments confirmed an increase in atresia and a corresponding reduction confined to follicle in early antral stage. As a mechanism of action, Con-A reduces ovarian proliferation, Von Willebrand staining, and angiogenesis. Based on the observation that Con-A may induce granulosa cell death followed by follicle death, our results further demonstrate that follicular carbohydrate moiety is changing under the influence of FSH, which may allow a carbohydrate- binding lectin to increase granulosa cell death. The physiological consequences of circulating lectin-like molecules remain to be determined. However, our results suggest a potential exploitation of carbohydrate binding in fertility and ovarian cancer treatment. This work may shed light on a key role of carbohydrates in the still obscure physiological process of follicular selection and atresia.Fil: Velasquez, Ethel V.. Pontificia Universidad Católica de Chile; ChileFil: Rios, Mariana. Pontificia Universidad Católica de Chile; ChileFil: Ortiz, María Elena. Pontificia Universidad Católica de Chile; ChileFil: Lizama, Carlos. Pontificia Universidad Católica de Chile; ChileFil: Nuñez, Elizabeth. Pontificia Universidad Católica de Chile; ChileFil: Abramovich, Dalhia Nurit. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Orge, Felipe. Pontificia Universidad Católica de Chile; ChileFil: Oliva, Bárbara. Pontificia Universidad Católica de Chile; ChileFil: Orellana, Renán. Pontificia Universidad Católica de Chile; ChileFil: Villalon, Manuel. Pontificia Universidad Católica de Chile; ChileFil: Moreno, Ricardo D.. Pontificia Universidad Católica de Chile; ChileFil: Tesone, Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Rokka, Anne. Universidad Andrés Bello; ChileFil: Corthals, Garry. Universidad Andrés Bello; ChileFil: Croxatto, Horacio B.. Universidad de Santiago de Chile; ChileFil: Parborell, Maria Fernanda Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Owen, Gareth I.. Pontificia Universidad Católica de Chile; ChileEndocrine Society2013-03-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/2438Velasquez, Ethel V.; Rios, Mariana; Ortiz, María Elena; Lizama, Carlos; Nuñez, Elizabeth; et al.; Concanavalin-A induces granulosa cell death and inhibits fsh-mediated follicular growth and ovarian maturation in female rats; Endocrine Society; Endocrinology; 154; 5; 20-3-2013; 1885-18960013-72271945-7170enginfo:eu-repo/semantics/altIdentifier/doi/10.1210/en.2012-1945info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/endo/article/154/5/1885/2423594info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:20Zoai:ri.conicet.gov.ar:11336/2438instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:20.775CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Concanavalin-A induces granulosa cell death and inhibits fsh-mediated follicular growth and ovarian maturation in female rats
title Concanavalin-A induces granulosa cell death and inhibits fsh-mediated follicular growth and ovarian maturation in female rats
spellingShingle Concanavalin-A induces granulosa cell death and inhibits fsh-mediated follicular growth and ovarian maturation in female rats
Velasquez, Ethel V.
Concanavalin A
Granulosa Cells
Apoptosis
Fsh
title_short Concanavalin-A induces granulosa cell death and inhibits fsh-mediated follicular growth and ovarian maturation in female rats
title_full Concanavalin-A induces granulosa cell death and inhibits fsh-mediated follicular growth and ovarian maturation in female rats
title_fullStr Concanavalin-A induces granulosa cell death and inhibits fsh-mediated follicular growth and ovarian maturation in female rats
title_full_unstemmed Concanavalin-A induces granulosa cell death and inhibits fsh-mediated follicular growth and ovarian maturation in female rats
title_sort Concanavalin-A induces granulosa cell death and inhibits fsh-mediated follicular growth and ovarian maturation in female rats
dc.creator.none.fl_str_mv Velasquez, Ethel V.
Rios, Mariana
Ortiz, María Elena
Lizama, Carlos
Nuñez, Elizabeth
Abramovich, Dalhia Nurit
Orge, Felipe
Oliva, Bárbara
Orellana, Renán
Villalon, Manuel
Moreno, Ricardo D.
Tesone, Marta
Rokka, Anne
Corthals, Garry
Croxatto, Horacio B.
Parborell, Maria Fernanda Agustina
Owen, Gareth I.
author Velasquez, Ethel V.
author_facet Velasquez, Ethel V.
Rios, Mariana
Ortiz, María Elena
Lizama, Carlos
Nuñez, Elizabeth
Abramovich, Dalhia Nurit
Orge, Felipe
Oliva, Bárbara
Orellana, Renán
Villalon, Manuel
Moreno, Ricardo D.
Tesone, Marta
Rokka, Anne
Corthals, Garry
Croxatto, Horacio B.
Parborell, Maria Fernanda Agustina
Owen, Gareth I.
author_role author
author2 Rios, Mariana
Ortiz, María Elena
Lizama, Carlos
Nuñez, Elizabeth
Abramovich, Dalhia Nurit
Orge, Felipe
Oliva, Bárbara
Orellana, Renán
Villalon, Manuel
Moreno, Ricardo D.
Tesone, Marta
Rokka, Anne
Corthals, Garry
Croxatto, Horacio B.
Parborell, Maria Fernanda Agustina
Owen, Gareth I.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Concanavalin A
Granulosa Cells
Apoptosis
Fsh
topic Concanavalin A
Granulosa Cells
Apoptosis
Fsh
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Reproductive success stems from a finely regulated balance between follicular maturation and atresia, in which the role of carbohydrate structure is poorly understood. Here, we describe for the first time a fraction of purified recombinant human FSH that is capable of bringing about the cell death of granulosa cells and preventing follicular maturation in a rat model. Further analysis by mass spectrometry revealed the presence of the lectin Concanavalin-A (Con-A) within this fraction of recombinant FSH. Using both the fractionated FSH and Con-A, the observed cell death was predominantly located to the granulosa cells. Ex vivo culture of rat follicles demonstrated that follicle degeneration occurred and resulted in the release of a denuded and deteriorated oocyte. Moreover, in vivo experiments confirmed an increase in atresia and a corresponding reduction confined to follicle in early antral stage. As a mechanism of action, Con-A reduces ovarian proliferation, Von Willebrand staining, and angiogenesis. Based on the observation that Con-A may induce granulosa cell death followed by follicle death, our results further demonstrate that follicular carbohydrate moiety is changing under the influence of FSH, which may allow a carbohydrate- binding lectin to increase granulosa cell death. The physiological consequences of circulating lectin-like molecules remain to be determined. However, our results suggest a potential exploitation of carbohydrate binding in fertility and ovarian cancer treatment. This work may shed light on a key role of carbohydrates in the still obscure physiological process of follicular selection and atresia.
Fil: Velasquez, Ethel V.. Pontificia Universidad Católica de Chile; Chile
Fil: Rios, Mariana. Pontificia Universidad Católica de Chile; Chile
Fil: Ortiz, María Elena. Pontificia Universidad Católica de Chile; Chile
Fil: Lizama, Carlos. Pontificia Universidad Católica de Chile; Chile
Fil: Nuñez, Elizabeth. Pontificia Universidad Católica de Chile; Chile
Fil: Abramovich, Dalhia Nurit. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Orge, Felipe. Pontificia Universidad Católica de Chile; Chile
Fil: Oliva, Bárbara. Pontificia Universidad Católica de Chile; Chile
Fil: Orellana, Renán. Pontificia Universidad Católica de Chile; Chile
Fil: Villalon, Manuel. Pontificia Universidad Católica de Chile; Chile
Fil: Moreno, Ricardo D.. Pontificia Universidad Católica de Chile; Chile
Fil: Tesone, Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Rokka, Anne. Universidad Andrés Bello; Chile
Fil: Corthals, Garry. Universidad Andrés Bello; Chile
Fil: Croxatto, Horacio B.. Universidad de Santiago de Chile; Chile
Fil: Parborell, Maria Fernanda Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Owen, Gareth I.. Pontificia Universidad Católica de Chile; Chile
description Reproductive success stems from a finely regulated balance between follicular maturation and atresia, in which the role of carbohydrate structure is poorly understood. Here, we describe for the first time a fraction of purified recombinant human FSH that is capable of bringing about the cell death of granulosa cells and preventing follicular maturation in a rat model. Further analysis by mass spectrometry revealed the presence of the lectin Concanavalin-A (Con-A) within this fraction of recombinant FSH. Using both the fractionated FSH and Con-A, the observed cell death was predominantly located to the granulosa cells. Ex vivo culture of rat follicles demonstrated that follicle degeneration occurred and resulted in the release of a denuded and deteriorated oocyte. Moreover, in vivo experiments confirmed an increase in atresia and a corresponding reduction confined to follicle in early antral stage. As a mechanism of action, Con-A reduces ovarian proliferation, Von Willebrand staining, and angiogenesis. Based on the observation that Con-A may induce granulosa cell death followed by follicle death, our results further demonstrate that follicular carbohydrate moiety is changing under the influence of FSH, which may allow a carbohydrate- binding lectin to increase granulosa cell death. The physiological consequences of circulating lectin-like molecules remain to be determined. However, our results suggest a potential exploitation of carbohydrate binding in fertility and ovarian cancer treatment. This work may shed light on a key role of carbohydrates in the still obscure physiological process of follicular selection and atresia.
publishDate 2013
dc.date.none.fl_str_mv 2013-03-20
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/2438
Velasquez, Ethel V.; Rios, Mariana; Ortiz, María Elena; Lizama, Carlos; Nuñez, Elizabeth; et al.; Concanavalin-A induces granulosa cell death and inhibits fsh-mediated follicular growth and ovarian maturation in female rats; Endocrine Society; Endocrinology; 154; 5; 20-3-2013; 1885-1896
0013-7227
1945-7170
url http://hdl.handle.net/11336/2438
identifier_str_mv Velasquez, Ethel V.; Rios, Mariana; Ortiz, María Elena; Lizama, Carlos; Nuñez, Elizabeth; et al.; Concanavalin-A induces granulosa cell death and inhibits fsh-mediated follicular growth and ovarian maturation in female rats; Endocrine Society; Endocrinology; 154; 5; 20-3-2013; 1885-1896
0013-7227
1945-7170
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1210/en.2012-1945
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/endo/article/154/5/1885/2423594
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Endocrine Society
publisher.none.fl_str_mv Endocrine Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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