The glycan structure in recombinant human FSH affects endocrine activity and global gene expression in human granulosa cells

Autores
Loreti, Nazareth; Fresno, Cristobal; Barrera, David; Andreone, Luz; Lira Albarran, Saúl; Fernandez, Elmer Andres; Larrea, Fernando; Campo, Stella Maris
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this study was to analyse the biological response to different recombinant human FSH (rhFSH)glycosylation variants on the endocrine activity and gene expression at whole-genome scale in human granulosa-like tumor cell line, KGN. The effects of differences in rhFSH sialylation and oligosaccharide complexity were determined on steroid hormone and inhibin production. A microarray approach was used to explore gene expression patterns induced by rhFSH glycosylation variants. Set enrichment analysis revealed that hormone sialylation and oligosaccharide complexity in rhFSH differentially affected the expression of genes involved in essential biological processes and molecular functions of KGN cells. The relevance of rhFSH oligosaccharide structure on teroidogenesis was confirmed assessing gene expression by real time-PCR. The results demonstrate that FSH oligosaccharide structure affects expression of genes encoding proteins, growth factors and hormones essential for granulosa cells function.
Fil: Loreti, Nazareth. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
Fil: Fresno, Cristobal. Universidad Católica de Córdoba. Facultad de Ingeniería. Biomedical Data Mining Group; Argentina
Fil: Barrera, David. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán. Departamento de Biología de la Reproducción; México
Fil: Andreone, Luz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
Fil: Lira Albarran, Saúl. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán. Departamento de Biología de la Reproducción; México
Fil: Fernandez, Elmer Andres. Universidad Católica de Córdoba. Facultad de Ingeniería. Biomedical Data Mining Group; Argentina
Fil: Larrea, Fernando. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán. Departamento de Biología de la Reproducción; México
Fil: Campo, Stella Maris. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
Materia
Fsh
Glycosylation Variant
Steroid
Inhibin
Gene Expression
Human Granulosa Cell
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/3108
Acceder
id CONICETDig_91204d4c29e79486505061440082e7e1
oai_identifier_str oai:ri.conicet.gov.ar:11336/3108
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The glycan structure in recombinant human FSH affects endocrine activity and global gene expression in human granulosa cellsLoreti, NazarethFresno, CristobalBarrera, DavidAndreone, LuzLira Albarran, SaúlFernandez, Elmer AndresLarrea, FernandoCampo, Stella MarisFshGlycosylation VariantSteroidInhibinGene ExpressionHuman Granulosa Cellhttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3The aim of this study was to analyse the biological response to different recombinant human FSH (rhFSH)glycosylation variants on the endocrine activity and gene expression at whole-genome scale in human granulosa-like tumor cell line, KGN. The effects of differences in rhFSH sialylation and oligosaccharide complexity were determined on steroid hormone and inhibin production. A microarray approach was used to explore gene expression patterns induced by rhFSH glycosylation variants. Set enrichment analysis revealed that hormone sialylation and oligosaccharide complexity in rhFSH differentially affected the expression of genes involved in essential biological processes and molecular functions of KGN cells. The relevance of rhFSH oligosaccharide structure on teroidogenesis was confirmed assessing gene expression by real time-PCR. The results demonstrate that FSH oligosaccharide structure affects expression of genes encoding proteins, growth factors and hormones essential for granulosa cells function.Fil: Loreti, Nazareth. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; ArgentinaFil: Fresno, Cristobal. Universidad Católica de Córdoba. Facultad de Ingeniería. Biomedical Data Mining Group; ArgentinaFil: Barrera, David. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán. Departamento de Biología de la Reproducción; MéxicoFil: Andreone, Luz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; ArgentinaFil: Lira Albarran, Saúl. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán. Departamento de Biología de la Reproducción; MéxicoFil: Fernandez, Elmer Andres. Universidad Católica de Córdoba. Facultad de Ingeniería. Biomedical Data Mining Group; ArgentinaFil: Larrea, Fernando. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán. Departamento de Biología de la Reproducción; MéxicoFil: Campo, Stella Maris. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; ArgentinaElsevier2013-12-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/3108Loreti, Nazareth; Fresno, Cristobal; Barrera, David; Andreone, Luz; Lira Albarran, Saúl; et al.; The glycan structure in recombinant human FSH affects endocrine activity and global gene expression in human granulosa cells; Elsevier; Molecular and Cellular Endocrinology; 366; 1; 5-12-2013; 68-800303-7207enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S030372071200528Xinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.mce.2012.11.021info:eu-repo/semantics/altIdentifier/issn/0303-7207info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:54:34Zoai:ri.conicet.gov.ar:11336/3108instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:54:35.259CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The glycan structure in recombinant human FSH affects endocrine activity and global gene expression in human granulosa cells
title The glycan structure in recombinant human FSH affects endocrine activity and global gene expression in human granulosa cells
spellingShingle The glycan structure in recombinant human FSH affects endocrine activity and global gene expression in human granulosa cells
Loreti, Nazareth
Fsh
Glycosylation Variant
Steroid
Inhibin
Gene Expression
Human Granulosa Cell
title_short The glycan structure in recombinant human FSH affects endocrine activity and global gene expression in human granulosa cells
title_full The glycan structure in recombinant human FSH affects endocrine activity and global gene expression in human granulosa cells
title_fullStr The glycan structure in recombinant human FSH affects endocrine activity and global gene expression in human granulosa cells
title_full_unstemmed The glycan structure in recombinant human FSH affects endocrine activity and global gene expression in human granulosa cells
title_sort The glycan structure in recombinant human FSH affects endocrine activity and global gene expression in human granulosa cells
dc.creator.none.fl_str_mv Loreti, Nazareth
Fresno, Cristobal
Barrera, David
Andreone, Luz
Lira Albarran, Saúl
Fernandez, Elmer Andres
Larrea, Fernando
Campo, Stella Maris
author Loreti, Nazareth
author_facet Loreti, Nazareth
Fresno, Cristobal
Barrera, David
Andreone, Luz
Lira Albarran, Saúl
Fernandez, Elmer Andres
Larrea, Fernando
Campo, Stella Maris
author_role author
author2 Fresno, Cristobal
Barrera, David
Andreone, Luz
Lira Albarran, Saúl
Fernandez, Elmer Andres
Larrea, Fernando
Campo, Stella Maris
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Fsh
Glycosylation Variant
Steroid
Inhibin
Gene Expression
Human Granulosa Cell
topic Fsh
Glycosylation Variant
Steroid
Inhibin
Gene Expression
Human Granulosa Cell
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The aim of this study was to analyse the biological response to different recombinant human FSH (rhFSH)glycosylation variants on the endocrine activity and gene expression at whole-genome scale in human granulosa-like tumor cell line, KGN. The effects of differences in rhFSH sialylation and oligosaccharide complexity were determined on steroid hormone and inhibin production. A microarray approach was used to explore gene expression patterns induced by rhFSH glycosylation variants. Set enrichment analysis revealed that hormone sialylation and oligosaccharide complexity in rhFSH differentially affected the expression of genes involved in essential biological processes and molecular functions of KGN cells. The relevance of rhFSH oligosaccharide structure on teroidogenesis was confirmed assessing gene expression by real time-PCR. The results demonstrate that FSH oligosaccharide structure affects expression of genes encoding proteins, growth factors and hormones essential for granulosa cells function.
Fil: Loreti, Nazareth. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
Fil: Fresno, Cristobal. Universidad Católica de Córdoba. Facultad de Ingeniería. Biomedical Data Mining Group; Argentina
Fil: Barrera, David. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán. Departamento de Biología de la Reproducción; México
Fil: Andreone, Luz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
Fil: Lira Albarran, Saúl. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán. Departamento de Biología de la Reproducción; México
Fil: Fernandez, Elmer Andres. Universidad Católica de Córdoba. Facultad de Ingeniería. Biomedical Data Mining Group; Argentina
Fil: Larrea, Fernando. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán. Departamento de Biología de la Reproducción; México
Fil: Campo, Stella Maris. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
description The aim of this study was to analyse the biological response to different recombinant human FSH (rhFSH)glycosylation variants on the endocrine activity and gene expression at whole-genome scale in human granulosa-like tumor cell line, KGN. The effects of differences in rhFSH sialylation and oligosaccharide complexity were determined on steroid hormone and inhibin production. A microarray approach was used to explore gene expression patterns induced by rhFSH glycosylation variants. Set enrichment analysis revealed that hormone sialylation and oligosaccharide complexity in rhFSH differentially affected the expression of genes involved in essential biological processes and molecular functions of KGN cells. The relevance of rhFSH oligosaccharide structure on teroidogenesis was confirmed assessing gene expression by real time-PCR. The results demonstrate that FSH oligosaccharide structure affects expression of genes encoding proteins, growth factors and hormones essential for granulosa cells function.
publishDate 2013
dc.date.none.fl_str_mv 2013-12-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/3108
Loreti, Nazareth; Fresno, Cristobal; Barrera, David; Andreone, Luz; Lira Albarran, Saúl; et al.; The glycan structure in recombinant human FSH affects endocrine activity and global gene expression in human granulosa cells; Elsevier; Molecular and Cellular Endocrinology; 366; 1; 5-12-2013; 68-80
0303-7207
url http://hdl.handle.net/11336/3108
identifier_str_mv Loreti, Nazareth; Fresno, Cristobal; Barrera, David; Andreone, Luz; Lira Albarran, Saúl; et al.; The glycan structure in recombinant human FSH affects endocrine activity and global gene expression in human granulosa cells; Elsevier; Molecular and Cellular Endocrinology; 366; 1; 5-12-2013; 68-80
0303-7207
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S030372071200528X
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.mce.2012.11.021
info:eu-repo/semantics/altIdentifier/issn/0303-7207
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613657009324032
score 13.069144

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