The glycan structure in recombinant human FSH affects endocrine activity and global gene expression in human granulosa cells
- Autores
- Loreti, Nazareth; Fresno, Cristobal; Barrera, David; Andreone, Luz; Lira Albarran, Saúl; Fernandez, Elmer Andres; Larrea, Fernando; Campo, Stella Maris
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The aim of this study was to analyse the biological response to different recombinant human FSH (rhFSH)glycosylation variants on the endocrine activity and gene expression at whole-genome scale in human granulosa-like tumor cell line, KGN. The effects of differences in rhFSH sialylation and oligosaccharide complexity were determined on steroid hormone and inhibin production. A microarray approach was used to explore gene expression patterns induced by rhFSH glycosylation variants. Set enrichment analysis revealed that hormone sialylation and oligosaccharide complexity in rhFSH differentially affected the expression of genes involved in essential biological processes and molecular functions of KGN cells. The relevance of rhFSH oligosaccharide structure on teroidogenesis was confirmed assessing gene expression by real time-PCR. The results demonstrate that FSH oligosaccharide structure affects expression of genes encoding proteins, growth factors and hormones essential for granulosa cells function.
Fil: Loreti, Nazareth. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
Fil: Fresno, Cristobal. Universidad Católica de Córdoba. Facultad de Ingeniería. Biomedical Data Mining Group; Argentina
Fil: Barrera, David. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán. Departamento de Biología de la Reproducción; México
Fil: Andreone, Luz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
Fil: Lira Albarran, Saúl. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán. Departamento de Biología de la Reproducción; México
Fil: Fernandez, Elmer Andres. Universidad Católica de Córdoba. Facultad de Ingeniería. Biomedical Data Mining Group; Argentina
Fil: Larrea, Fernando. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán. Departamento de Biología de la Reproducción; México
Fil: Campo, Stella Maris. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina - Materia
-
Fsh
Glycosylation Variant
Steroid
Inhibin
Gene Expression
Human Granulosa Cell - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/3108
Ver los metadatos del registro completo
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oai:ri.conicet.gov.ar:11336/3108 |
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spelling |
The glycan structure in recombinant human FSH affects endocrine activity and global gene expression in human granulosa cellsLoreti, NazarethFresno, CristobalBarrera, DavidAndreone, LuzLira Albarran, SaúlFernandez, Elmer AndresLarrea, FernandoCampo, Stella MarisFshGlycosylation VariantSteroidInhibinGene ExpressionHuman Granulosa Cellhttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3The aim of this study was to analyse the biological response to different recombinant human FSH (rhFSH)glycosylation variants on the endocrine activity and gene expression at whole-genome scale in human granulosa-like tumor cell line, KGN. The effects of differences in rhFSH sialylation and oligosaccharide complexity were determined on steroid hormone and inhibin production. A microarray approach was used to explore gene expression patterns induced by rhFSH glycosylation variants. Set enrichment analysis revealed that hormone sialylation and oligosaccharide complexity in rhFSH differentially affected the expression of genes involved in essential biological processes and molecular functions of KGN cells. The relevance of rhFSH oligosaccharide structure on teroidogenesis was confirmed assessing gene expression by real time-PCR. The results demonstrate that FSH oligosaccharide structure affects expression of genes encoding proteins, growth factors and hormones essential for granulosa cells function.Fil: Loreti, Nazareth. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; ArgentinaFil: Fresno, Cristobal. Universidad Católica de Córdoba. Facultad de Ingeniería. Biomedical Data Mining Group; ArgentinaFil: Barrera, David. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán. Departamento de Biología de la Reproducción; MéxicoFil: Andreone, Luz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; ArgentinaFil: Lira Albarran, Saúl. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán. Departamento de Biología de la Reproducción; MéxicoFil: Fernandez, Elmer Andres. Universidad Católica de Córdoba. Facultad de Ingeniería. Biomedical Data Mining Group; ArgentinaFil: Larrea, Fernando. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán. Departamento de Biología de la Reproducción; MéxicoFil: Campo, Stella Maris. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; ArgentinaElsevier2013-12-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/3108Loreti, Nazareth; Fresno, Cristobal; Barrera, David; Andreone, Luz; Lira Albarran, Saúl; et al.; The glycan structure in recombinant human FSH affects endocrine activity and global gene expression in human granulosa cells; Elsevier; Molecular and Cellular Endocrinology; 366; 1; 5-12-2013; 68-800303-7207enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S030372071200528Xinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.mce.2012.11.021info:eu-repo/semantics/altIdentifier/issn/0303-7207info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:54:09Zoai:ri.conicet.gov.ar:11336/3108instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:54:10.174CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
The glycan structure in recombinant human FSH affects endocrine activity and global gene expression in human granulosa cells |
title |
The glycan structure in recombinant human FSH affects endocrine activity and global gene expression in human granulosa cells |
spellingShingle |
The glycan structure in recombinant human FSH affects endocrine activity and global gene expression in human granulosa cells Loreti, Nazareth Fsh Glycosylation Variant Steroid Inhibin Gene Expression Human Granulosa Cell |
title_short |
The glycan structure in recombinant human FSH affects endocrine activity and global gene expression in human granulosa cells |
title_full |
The glycan structure in recombinant human FSH affects endocrine activity and global gene expression in human granulosa cells |
title_fullStr |
The glycan structure in recombinant human FSH affects endocrine activity and global gene expression in human granulosa cells |
title_full_unstemmed |
The glycan structure in recombinant human FSH affects endocrine activity and global gene expression in human granulosa cells |
title_sort |
The glycan structure in recombinant human FSH affects endocrine activity and global gene expression in human granulosa cells |
dc.creator.none.fl_str_mv |
Loreti, Nazareth Fresno, Cristobal Barrera, David Andreone, Luz Lira Albarran, Saúl Fernandez, Elmer Andres Larrea, Fernando Campo, Stella Maris |
author |
Loreti, Nazareth |
author_facet |
Loreti, Nazareth Fresno, Cristobal Barrera, David Andreone, Luz Lira Albarran, Saúl Fernandez, Elmer Andres Larrea, Fernando Campo, Stella Maris |
author_role |
author |
author2 |
Fresno, Cristobal Barrera, David Andreone, Luz Lira Albarran, Saúl Fernandez, Elmer Andres Larrea, Fernando Campo, Stella Maris |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
Fsh Glycosylation Variant Steroid Inhibin Gene Expression Human Granulosa Cell |
topic |
Fsh Glycosylation Variant Steroid Inhibin Gene Expression Human Granulosa Cell |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.2 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
The aim of this study was to analyse the biological response to different recombinant human FSH (rhFSH)glycosylation variants on the endocrine activity and gene expression at whole-genome scale in human granulosa-like tumor cell line, KGN. The effects of differences in rhFSH sialylation and oligosaccharide complexity were determined on steroid hormone and inhibin production. A microarray approach was used to explore gene expression patterns induced by rhFSH glycosylation variants. Set enrichment analysis revealed that hormone sialylation and oligosaccharide complexity in rhFSH differentially affected the expression of genes involved in essential biological processes and molecular functions of KGN cells. The relevance of rhFSH oligosaccharide structure on teroidogenesis was confirmed assessing gene expression by real time-PCR. The results demonstrate that FSH oligosaccharide structure affects expression of genes encoding proteins, growth factors and hormones essential for granulosa cells function. Fil: Loreti, Nazareth. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina Fil: Fresno, Cristobal. Universidad Católica de Córdoba. Facultad de Ingeniería. Biomedical Data Mining Group; Argentina Fil: Barrera, David. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán. Departamento de Biología de la Reproducción; México Fil: Andreone, Luz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina Fil: Lira Albarran, Saúl. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán. Departamento de Biología de la Reproducción; México Fil: Fernandez, Elmer Andres. Universidad Católica de Córdoba. Facultad de Ingeniería. Biomedical Data Mining Group; Argentina Fil: Larrea, Fernando. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán. Departamento de Biología de la Reproducción; México Fil: Campo, Stella Maris. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina |
description |
The aim of this study was to analyse the biological response to different recombinant human FSH (rhFSH)glycosylation variants on the endocrine activity and gene expression at whole-genome scale in human granulosa-like tumor cell line, KGN. The effects of differences in rhFSH sialylation and oligosaccharide complexity were determined on steroid hormone and inhibin production. A microarray approach was used to explore gene expression patterns induced by rhFSH glycosylation variants. Set enrichment analysis revealed that hormone sialylation and oligosaccharide complexity in rhFSH differentially affected the expression of genes involved in essential biological processes and molecular functions of KGN cells. The relevance of rhFSH oligosaccharide structure on teroidogenesis was confirmed assessing gene expression by real time-PCR. The results demonstrate that FSH oligosaccharide structure affects expression of genes encoding proteins, growth factors and hormones essential for granulosa cells function. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-12-05 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/3108 Loreti, Nazareth; Fresno, Cristobal; Barrera, David; Andreone, Luz; Lira Albarran, Saúl; et al.; The glycan structure in recombinant human FSH affects endocrine activity and global gene expression in human granulosa cells; Elsevier; Molecular and Cellular Endocrinology; 366; 1; 5-12-2013; 68-80 0303-7207 |
url |
http://hdl.handle.net/11336/3108 |
identifier_str_mv |
Loreti, Nazareth; Fresno, Cristobal; Barrera, David; Andreone, Luz; Lira Albarran, Saúl; et al.; The glycan structure in recombinant human FSH affects endocrine activity and global gene expression in human granulosa cells; Elsevier; Molecular and Cellular Endocrinology; 366; 1; 5-12-2013; 68-80 0303-7207 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S030372071200528X info:eu-repo/semantics/altIdentifier/doi/10.1016/j.mce.2012.11.021 info:eu-repo/semantics/altIdentifier/issn/0303-7207 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842269268947763200 |
score |
13.13397 |