Epithelial cancer biomarker EpCAM determination in peripheral blood samples using a microfluidic immunosensor based in silver nanoparticles as platform

Autores
Ortega, Francisco G.; Fernández Baldo, Martín Alejandro; Serrano, María J.; Messina, Germán Alejandro; Lorente, José A.; Raba, Julio
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In the present work, we report a microfluidic immunosensor for epithelial cancer biomarker EpCAM (epithelial cell adhesion molecule) determination. It is based on the use of synthetized silver nanoparticles (AgNPs) covered by chitosan (Cts). AgNPs-Cts were covalently attached to the central channel (CC) of the microfluidic immunosensor. These nanoparticles were employed as platform for anti-EpCAM monoclonal antibodies immobilization for specifically recognize and capture EpCAM in peripheral blood samples. Afterwards, the amount of this trapped epithelial cancer biomarker was quantified by HRP-conjugated anti-EpCAM-antibody. HRP reacted with its enzymatic substrate in a redox process which resulted in the appearance of a current whose magnitude was directly proportional to the level of EpCAM in the peripheral blood sample. The structure and morphology of synthetized AgNPs-Cts were characterized by UV-visible spectroscopy, scanning electron microscopy (SEM), energy dispersive spectrometer (EDS) and X-ray diffraction (XRD). The calculated detection limits for microfluidic immunosensor and the commercial ELISA were 2.7 and 13.9 pg mL-1, respectively and the within- and between-assay coefficients of variation were below 6.37% for the proposed method. The microfluidic immunosensor is simple, sensitive, specific and reproducible. It has the potential for reliable point-of-care clinical diagnosis and prognosis of epithelial origin tumors in biological samples.
Fil: Ortega, Francisco G.. Universidad de Granada; España
Fil: Fernández Baldo, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Serrano, María J.. Universidad de Granada; España
Fil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Lorente, José A.. Universidad de Granada; España
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Materia
Circulating Tumor Cells
Epcam
Epithelial Cancer
Microfluidic Immunosensor
Platform
Silver Nanoparticles
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/50097
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/50097
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Epithelial cancer biomarker EpCAM determination in peripheral blood samples using a microfluidic immunosensor based in silver nanoparticles as platformOrtega, Francisco G.Fernández Baldo, Martín AlejandroSerrano, María J.Messina, Germán AlejandroLorente, José A.Raba, JulioCirculating Tumor CellsEpcamEpithelial CancerMicrofluidic ImmunosensorPlatformSilver Nanoparticleshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1In the present work, we report a microfluidic immunosensor for epithelial cancer biomarker EpCAM (epithelial cell adhesion molecule) determination. It is based on the use of synthetized silver nanoparticles (AgNPs) covered by chitosan (Cts). AgNPs-Cts were covalently attached to the central channel (CC) of the microfluidic immunosensor. These nanoparticles were employed as platform for anti-EpCAM monoclonal antibodies immobilization for specifically recognize and capture EpCAM in peripheral blood samples. Afterwards, the amount of this trapped epithelial cancer biomarker was quantified by HRP-conjugated anti-EpCAM-antibody. HRP reacted with its enzymatic substrate in a redox process which resulted in the appearance of a current whose magnitude was directly proportional to the level of EpCAM in the peripheral blood sample. The structure and morphology of synthetized AgNPs-Cts were characterized by UV-visible spectroscopy, scanning electron microscopy (SEM), energy dispersive spectrometer (EDS) and X-ray diffraction (XRD). The calculated detection limits for microfluidic immunosensor and the commercial ELISA were 2.7 and 13.9 pg mL-1, respectively and the within- and between-assay coefficients of variation were below 6.37% for the proposed method. The microfluidic immunosensor is simple, sensitive, specific and reproducible. It has the potential for reliable point-of-care clinical diagnosis and prognosis of epithelial origin tumors in biological samples.Fil: Ortega, Francisco G.. Universidad de Granada; EspañaFil: Fernández Baldo, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Serrano, María J.. Universidad de Granada; EspañaFil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Lorente, José A.. Universidad de Granada; EspañaFil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaElsevier Science Sa2015-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/50097Ortega, Francisco G.; Fernández Baldo, Martín Alejandro; Serrano, María J.; Messina, Germán Alejandro; Lorente, José A.; et al.; Epithelial cancer biomarker EpCAM determination in peripheral blood samples using a microfluidic immunosensor based in silver nanoparticles as platform; Elsevier Science Sa; Sensors and Actuators B: Chemical; 221; 7-2015; 248-2560925-4005CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0925400515008412info:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2015.06.066info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:48:20Zoai:ri.conicet.gov.ar:11336/50097instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:48:20.861CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Epithelial cancer biomarker EpCAM determination in peripheral blood samples using a microfluidic immunosensor based in silver nanoparticles as platform
title Epithelial cancer biomarker EpCAM determination in peripheral blood samples using a microfluidic immunosensor based in silver nanoparticles as platform
spellingShingle Epithelial cancer biomarker EpCAM determination in peripheral blood samples using a microfluidic immunosensor based in silver nanoparticles as platform
Ortega, Francisco G.
Circulating Tumor Cells
Epcam
Epithelial Cancer
Microfluidic Immunosensor
Platform
Silver Nanoparticles
title_short Epithelial cancer biomarker EpCAM determination in peripheral blood samples using a microfluidic immunosensor based in silver nanoparticles as platform
title_full Epithelial cancer biomarker EpCAM determination in peripheral blood samples using a microfluidic immunosensor based in silver nanoparticles as platform
title_fullStr Epithelial cancer biomarker EpCAM determination in peripheral blood samples using a microfluidic immunosensor based in silver nanoparticles as platform
title_full_unstemmed Epithelial cancer biomarker EpCAM determination in peripheral blood samples using a microfluidic immunosensor based in silver nanoparticles as platform
title_sort Epithelial cancer biomarker EpCAM determination in peripheral blood samples using a microfluidic immunosensor based in silver nanoparticles as platform
dc.creator.none.fl_str_mv Ortega, Francisco G.
Fernández Baldo, Martín Alejandro
Serrano, María J.
Messina, Germán Alejandro
Lorente, José A.
Raba, Julio
author Ortega, Francisco G.
author_facet Ortega, Francisco G.
Fernández Baldo, Martín Alejandro
Serrano, María J.
Messina, Germán Alejandro
Lorente, José A.
Raba, Julio
author_role author
author2 Fernández Baldo, Martín Alejandro
Serrano, María J.
Messina, Germán Alejandro
Lorente, José A.
Raba, Julio
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Circulating Tumor Cells
Epcam
Epithelial Cancer
Microfluidic Immunosensor
Platform
Silver Nanoparticles
topic Circulating Tumor Cells
Epcam
Epithelial Cancer
Microfluidic Immunosensor
Platform
Silver Nanoparticles
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In the present work, we report a microfluidic immunosensor for epithelial cancer biomarker EpCAM (epithelial cell adhesion molecule) determination. It is based on the use of synthetized silver nanoparticles (AgNPs) covered by chitosan (Cts). AgNPs-Cts were covalently attached to the central channel (CC) of the microfluidic immunosensor. These nanoparticles were employed as platform for anti-EpCAM monoclonal antibodies immobilization for specifically recognize and capture EpCAM in peripheral blood samples. Afterwards, the amount of this trapped epithelial cancer biomarker was quantified by HRP-conjugated anti-EpCAM-antibody. HRP reacted with its enzymatic substrate in a redox process which resulted in the appearance of a current whose magnitude was directly proportional to the level of EpCAM in the peripheral blood sample. The structure and morphology of synthetized AgNPs-Cts were characterized by UV-visible spectroscopy, scanning electron microscopy (SEM), energy dispersive spectrometer (EDS) and X-ray diffraction (XRD). The calculated detection limits for microfluidic immunosensor and the commercial ELISA were 2.7 and 13.9 pg mL-1, respectively and the within- and between-assay coefficients of variation were below 6.37% for the proposed method. The microfluidic immunosensor is simple, sensitive, specific and reproducible. It has the potential for reliable point-of-care clinical diagnosis and prognosis of epithelial origin tumors in biological samples.
Fil: Ortega, Francisco G.. Universidad de Granada; España
Fil: Fernández Baldo, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Serrano, María J.. Universidad de Granada; España
Fil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Lorente, José A.. Universidad de Granada; España
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
description In the present work, we report a microfluidic immunosensor for epithelial cancer biomarker EpCAM (epithelial cell adhesion molecule) determination. It is based on the use of synthetized silver nanoparticles (AgNPs) covered by chitosan (Cts). AgNPs-Cts were covalently attached to the central channel (CC) of the microfluidic immunosensor. These nanoparticles were employed as platform for anti-EpCAM monoclonal antibodies immobilization for specifically recognize and capture EpCAM in peripheral blood samples. Afterwards, the amount of this trapped epithelial cancer biomarker was quantified by HRP-conjugated anti-EpCAM-antibody. HRP reacted with its enzymatic substrate in a redox process which resulted in the appearance of a current whose magnitude was directly proportional to the level of EpCAM in the peripheral blood sample. The structure and morphology of synthetized AgNPs-Cts were characterized by UV-visible spectroscopy, scanning electron microscopy (SEM), energy dispersive spectrometer (EDS) and X-ray diffraction (XRD). The calculated detection limits for microfluidic immunosensor and the commercial ELISA were 2.7 and 13.9 pg mL-1, respectively and the within- and between-assay coefficients of variation were below 6.37% for the proposed method. The microfluidic immunosensor is simple, sensitive, specific and reproducible. It has the potential for reliable point-of-care clinical diagnosis and prognosis of epithelial origin tumors in biological samples.
publishDate 2015
dc.date.none.fl_str_mv 2015-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/50097
Ortega, Francisco G.; Fernández Baldo, Martín Alejandro; Serrano, María J.; Messina, Germán Alejandro; Lorente, José A.; et al.; Epithelial cancer biomarker EpCAM determination in peripheral blood samples using a microfluidic immunosensor based in silver nanoparticles as platform; Elsevier Science Sa; Sensors and Actuators B: Chemical; 221; 7-2015; 248-256
0925-4005
CONICET Digital
CONICET
url http://hdl.handle.net/11336/50097
identifier_str_mv Ortega, Francisco G.; Fernández Baldo, Martín Alejandro; Serrano, María J.; Messina, Germán Alejandro; Lorente, José A.; et al.; Epithelial cancer biomarker EpCAM determination in peripheral blood samples using a microfluidic immunosensor based in silver nanoparticles as platform; Elsevier Science Sa; Sensors and Actuators B: Chemical; 221; 7-2015; 248-256
0925-4005
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0925400515008412
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2015.06.066
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Sa
publisher.none.fl_str_mv Elsevier Science Sa
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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