Development of a nanostructured electrochemical immunosensor applied to the early detection of invasive aspergillosis

Autores
Piguillem Palacios, Sofía Viviana; Ortega Sánchez, Francisco Gabriel; Raba, Julio; Messina, Germán Alejandro; Fernández Baldo, Martín Alejandro
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The use of a biological marker as an adjunct for screening for invasive aspergillosis (IA) in high-risk patients is attractive, because it is noninvasive and may detect evidence of IA prior to the appearance of clinical signs and symptoms. Galactomannan (GMN) is a polysaccharide cell-wall component that is released by Aspergillus species. In the present work, an electrochemical microfluidic immunosensor for GMN detection was developed. Moreover, copper nanoparticles covered with polyvinylpyrrolidone (CuNPs-PVP) were synthesized, characterized and used in a microfluidic immunosensor based on the use of anti-GMN antibodies as a trapping agent. The concentration of trapped GMN is then electrochemically quantified by HRP-conjugated anti-GMN-antibody. HRP reacted with its enzymatic substrate in a redox process which resulted in the appearance of a current whose magnitude is directly proportional to the concentration of GMN. The immunosensor was validated by analyzing patient samples, and a good correlation with a commercial ELISA was obtained. This microfluidic immunosensor can be applied to the early diagnosis of IA.
Fil: Piguillem Palacios, Sofía Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Ortega Sánchez, Francisco Gabriel. Weizmann Institute of Science; Israel
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Fernández Baldo, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Materia
CLINICAL DIAGNOSIS
ELECTROCHEMICAL
GALACTOMANNAN
INVASIVE ASPERGILLOSIS
MICROFLUIDIC IMMUNOSENSOR
NANO-PLATFORM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/87333
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/87333
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Development of a nanostructured electrochemical immunosensor applied to the early detection of invasive aspergillosisPiguillem Palacios, Sofía VivianaOrtega Sánchez, Francisco GabrielRaba, JulioMessina, Germán AlejandroFernández Baldo, Martín AlejandroCLINICAL DIAGNOSISELECTROCHEMICALGALACTOMANNANINVASIVE ASPERGILLOSISMICROFLUIDIC IMMUNOSENSORNANO-PLATFORMhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The use of a biological marker as an adjunct for screening for invasive aspergillosis (IA) in high-risk patients is attractive, because it is noninvasive and may detect evidence of IA prior to the appearance of clinical signs and symptoms. Galactomannan (GMN) is a polysaccharide cell-wall component that is released by Aspergillus species. In the present work, an electrochemical microfluidic immunosensor for GMN detection was developed. Moreover, copper nanoparticles covered with polyvinylpyrrolidone (CuNPs-PVP) were synthesized, characterized and used in a microfluidic immunosensor based on the use of anti-GMN antibodies as a trapping agent. The concentration of trapped GMN is then electrochemically quantified by HRP-conjugated anti-GMN-antibody. HRP reacted with its enzymatic substrate in a redox process which resulted in the appearance of a current whose magnitude is directly proportional to the concentration of GMN. The immunosensor was validated by analyzing patient samples, and a good correlation with a commercial ELISA was obtained. This microfluidic immunosensor can be applied to the early diagnosis of IA.Fil: Piguillem Palacios, Sofía Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Ortega Sánchez, Francisco Gabriel. Weizmann Institute of Science; IsraelFil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Fernández Baldo, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaElsevier Science2018-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/87333Piguillem Palacios, Sofía Viviana; Ortega Sánchez, Francisco Gabriel; Raba, Julio; Messina, Germán Alejandro; Fernández Baldo, Martín Alejandro; Development of a nanostructured electrochemical immunosensor applied to the early detection of invasive aspergillosis; Elsevier Science; Microchemical Journal; 139; 6-2018; 394-4000026-265XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0026265X17313358info:eu-repo/semantics/altIdentifier/doi/10.1016/j.microc.2018.03.025info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:11:12Zoai:ri.conicet.gov.ar:11336/87333instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:11:12.94CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Development of a nanostructured electrochemical immunosensor applied to the early detection of invasive aspergillosis
title Development of a nanostructured electrochemical immunosensor applied to the early detection of invasive aspergillosis
spellingShingle Development of a nanostructured electrochemical immunosensor applied to the early detection of invasive aspergillosis
Piguillem Palacios, Sofía Viviana
CLINICAL DIAGNOSIS
ELECTROCHEMICAL
GALACTOMANNAN
INVASIVE ASPERGILLOSIS
MICROFLUIDIC IMMUNOSENSOR
NANO-PLATFORM
title_short Development of a nanostructured electrochemical immunosensor applied to the early detection of invasive aspergillosis
title_full Development of a nanostructured electrochemical immunosensor applied to the early detection of invasive aspergillosis
title_fullStr Development of a nanostructured electrochemical immunosensor applied to the early detection of invasive aspergillosis
title_full_unstemmed Development of a nanostructured electrochemical immunosensor applied to the early detection of invasive aspergillosis
title_sort Development of a nanostructured electrochemical immunosensor applied to the early detection of invasive aspergillosis
dc.creator.none.fl_str_mv Piguillem Palacios, Sofía Viviana
Ortega Sánchez, Francisco Gabriel
Raba, Julio
Messina, Germán Alejandro
Fernández Baldo, Martín Alejandro
author Piguillem Palacios, Sofía Viviana
author_facet Piguillem Palacios, Sofía Viviana
Ortega Sánchez, Francisco Gabriel
Raba, Julio
Messina, Germán Alejandro
Fernández Baldo, Martín Alejandro
author_role author
author2 Ortega Sánchez, Francisco Gabriel
Raba, Julio
Messina, Germán Alejandro
Fernández Baldo, Martín Alejandro
author2_role author
author
author
author
dc.subject.none.fl_str_mv CLINICAL DIAGNOSIS
ELECTROCHEMICAL
GALACTOMANNAN
INVASIVE ASPERGILLOSIS
MICROFLUIDIC IMMUNOSENSOR
NANO-PLATFORM
topic CLINICAL DIAGNOSIS
ELECTROCHEMICAL
GALACTOMANNAN
INVASIVE ASPERGILLOSIS
MICROFLUIDIC IMMUNOSENSOR
NANO-PLATFORM
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The use of a biological marker as an adjunct for screening for invasive aspergillosis (IA) in high-risk patients is attractive, because it is noninvasive and may detect evidence of IA prior to the appearance of clinical signs and symptoms. Galactomannan (GMN) is a polysaccharide cell-wall component that is released by Aspergillus species. In the present work, an electrochemical microfluidic immunosensor for GMN detection was developed. Moreover, copper nanoparticles covered with polyvinylpyrrolidone (CuNPs-PVP) were synthesized, characterized and used in a microfluidic immunosensor based on the use of anti-GMN antibodies as a trapping agent. The concentration of trapped GMN is then electrochemically quantified by HRP-conjugated anti-GMN-antibody. HRP reacted with its enzymatic substrate in a redox process which resulted in the appearance of a current whose magnitude is directly proportional to the concentration of GMN. The immunosensor was validated by analyzing patient samples, and a good correlation with a commercial ELISA was obtained. This microfluidic immunosensor can be applied to the early diagnosis of IA.
Fil: Piguillem Palacios, Sofía Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Ortega Sánchez, Francisco Gabriel. Weizmann Institute of Science; Israel
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Fernández Baldo, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
description The use of a biological marker as an adjunct for screening for invasive aspergillosis (IA) in high-risk patients is attractive, because it is noninvasive and may detect evidence of IA prior to the appearance of clinical signs and symptoms. Galactomannan (GMN) is a polysaccharide cell-wall component that is released by Aspergillus species. In the present work, an electrochemical microfluidic immunosensor for GMN detection was developed. Moreover, copper nanoparticles covered with polyvinylpyrrolidone (CuNPs-PVP) were synthesized, characterized and used in a microfluidic immunosensor based on the use of anti-GMN antibodies as a trapping agent. The concentration of trapped GMN is then electrochemically quantified by HRP-conjugated anti-GMN-antibody. HRP reacted with its enzymatic substrate in a redox process which resulted in the appearance of a current whose magnitude is directly proportional to the concentration of GMN. The immunosensor was validated by analyzing patient samples, and a good correlation with a commercial ELISA was obtained. This microfluidic immunosensor can be applied to the early diagnosis of IA.
publishDate 2018
dc.date.none.fl_str_mv 2018-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/87333
Piguillem Palacios, Sofía Viviana; Ortega Sánchez, Francisco Gabriel; Raba, Julio; Messina, Germán Alejandro; Fernández Baldo, Martín Alejandro; Development of a nanostructured electrochemical immunosensor applied to the early detection of invasive aspergillosis; Elsevier Science; Microchemical Journal; 139; 6-2018; 394-400
0026-265X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/87333
identifier_str_mv Piguillem Palacios, Sofía Viviana; Ortega Sánchez, Francisco Gabriel; Raba, Julio; Messina, Germán Alejandro; Fernández Baldo, Martín Alejandro; Development of a nanostructured electrochemical immunosensor applied to the early detection of invasive aspergillosis; Elsevier Science; Microchemical Journal; 139; 6-2018; 394-400
0026-265X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0026265X17313358
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.microc.2018.03.025
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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