Nanostructured platform integrated into a microfluidic immunosensor coupled to laser-induced fluorescence for the epithelial cancer biomarker determination

Autores
Fernández Baldo, Martín Alejandro; Ortega, Francisco G.; Pereira, Sirley Vanesa; Bertolino, Franco Adrián; Serrano, María J.; Lorente, José A.; Raba, Julio; Messina, Germán Alejandro
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In the present work, we report a microfluidic immunosensor with a nanostructured platform based in zinc oxide nanoparticles covered by polyvinyl alcohol (ZnONPs-PVA) coupled to laser-induced fluorescence (LIF) for detection of epithelial cell adhesion molecule (EpCAM). EpCAM is a cell surface protein, and is overexpressed by epithelial carcinomas as such as the lung, colorectal, breast, prostate, head, neck, and hepatic origin. The detection limits (LODs) calculated for our method and for a commercial enzyme-linked immunosorbent assay (ELISA) test kit were 1.2 and 13.9 pg mL-1, respectively. The within- and between-assay variation coefficients for the proposed method were below 6.50%. The results correlated well with those obtained with the commercial ELISA method, thus demonstrating that the new nano-platform integrated into a microfluidic device offers a truthful and useful analytical tool to be easily applied in epithelial cancer diagnosis through EpCAM biomarker determination.
Fil: Fernández Baldo, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Ortega, Francisco G.. Universidad de Granada; España
Fil: Pereira, Sirley Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Bertolino, Franco Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Serrano, María J.. Universidad de Granada; España
Fil: Lorente, José A.. Universidad de Granada; España
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Materia
CANCER DIAGNOSTICS
CIRCULATING TUMOR CELLS
EPCAM BIOMARKER
EPITHELIAL CANCER
MICROFLUIDIC IMMUNOSENSOR
NANOSTRUCTURED PLATFORM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/60584
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/60584
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Nanostructured platform integrated into a microfluidic immunosensor coupled to laser-induced fluorescence for the epithelial cancer biomarker determinationFernández Baldo, Martín AlejandroOrtega, Francisco G.Pereira, Sirley VanesaBertolino, Franco AdriánSerrano, María J.Lorente, José A.Raba, JulioMessina, Germán AlejandroCANCER DIAGNOSTICSCIRCULATING TUMOR CELLSEPCAM BIOMARKEREPITHELIAL CANCERMICROFLUIDIC IMMUNOSENSORNANOSTRUCTURED PLATFORMhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1In the present work, we report a microfluidic immunosensor with a nanostructured platform based in zinc oxide nanoparticles covered by polyvinyl alcohol (ZnONPs-PVA) coupled to laser-induced fluorescence (LIF) for detection of epithelial cell adhesion molecule (EpCAM). EpCAM is a cell surface protein, and is overexpressed by epithelial carcinomas as such as the lung, colorectal, breast, prostate, head, neck, and hepatic origin. The detection limits (LODs) calculated for our method and for a commercial enzyme-linked immunosorbent assay (ELISA) test kit were 1.2 and 13.9 pg mL-1, respectively. The within- and between-assay variation coefficients for the proposed method were below 6.50%. The results correlated well with those obtained with the commercial ELISA method, thus demonstrating that the new nano-platform integrated into a microfluidic device offers a truthful and useful analytical tool to be easily applied in epithelial cancer diagnosis through EpCAM biomarker determination.Fil: Fernández Baldo, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Ortega, Francisco G.. Universidad de Granada; EspañaFil: Pereira, Sirley Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Bertolino, Franco Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Serrano, María J.. Universidad de Granada; EspañaFil: Lorente, José A.. Universidad de Granada; EspañaFil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaElsevier Science2016-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/60584Fernández Baldo, Martín Alejandro; Ortega, Francisco G.; Pereira, Sirley Vanesa; Bertolino, Franco Adrián; Serrano, María J.; et al.; Nanostructured platform integrated into a microfluidic immunosensor coupled to laser-induced fluorescence for the epithelial cancer biomarker determination; Elsevier Science; Microchemical Journal; 128; 9-2016; 18-250026-265XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0026265X16300091info:eu-repo/semantics/altIdentifier/doi/10.1016/j.microc.2016.03.012info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:29Zoai:ri.conicet.gov.ar:11336/60584instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:29.864CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Nanostructured platform integrated into a microfluidic immunosensor coupled to laser-induced fluorescence for the epithelial cancer biomarker determination
title Nanostructured platform integrated into a microfluidic immunosensor coupled to laser-induced fluorescence for the epithelial cancer biomarker determination
spellingShingle Nanostructured platform integrated into a microfluidic immunosensor coupled to laser-induced fluorescence for the epithelial cancer biomarker determination
Fernández Baldo, Martín Alejandro
CANCER DIAGNOSTICS
CIRCULATING TUMOR CELLS
EPCAM BIOMARKER
EPITHELIAL CANCER
MICROFLUIDIC IMMUNOSENSOR
NANOSTRUCTURED PLATFORM
title_short Nanostructured platform integrated into a microfluidic immunosensor coupled to laser-induced fluorescence for the epithelial cancer biomarker determination
title_full Nanostructured platform integrated into a microfluidic immunosensor coupled to laser-induced fluorescence for the epithelial cancer biomarker determination
title_fullStr Nanostructured platform integrated into a microfluidic immunosensor coupled to laser-induced fluorescence for the epithelial cancer biomarker determination
title_full_unstemmed Nanostructured platform integrated into a microfluidic immunosensor coupled to laser-induced fluorescence for the epithelial cancer biomarker determination
title_sort Nanostructured platform integrated into a microfluidic immunosensor coupled to laser-induced fluorescence for the epithelial cancer biomarker determination
dc.creator.none.fl_str_mv Fernández Baldo, Martín Alejandro
Ortega, Francisco G.
Pereira, Sirley Vanesa
Bertolino, Franco Adrián
Serrano, María J.
Lorente, José A.
Raba, Julio
Messina, Germán Alejandro
author Fernández Baldo, Martín Alejandro
author_facet Fernández Baldo, Martín Alejandro
Ortega, Francisco G.
Pereira, Sirley Vanesa
Bertolino, Franco Adrián
Serrano, María J.
Lorente, José A.
Raba, Julio
Messina, Germán Alejandro
author_role author
author2 Ortega, Francisco G.
Pereira, Sirley Vanesa
Bertolino, Franco Adrián
Serrano, María J.
Lorente, José A.
Raba, Julio
Messina, Germán Alejandro
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CANCER DIAGNOSTICS
CIRCULATING TUMOR CELLS
EPCAM BIOMARKER
EPITHELIAL CANCER
MICROFLUIDIC IMMUNOSENSOR
NANOSTRUCTURED PLATFORM
topic CANCER DIAGNOSTICS
CIRCULATING TUMOR CELLS
EPCAM BIOMARKER
EPITHELIAL CANCER
MICROFLUIDIC IMMUNOSENSOR
NANOSTRUCTURED PLATFORM
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In the present work, we report a microfluidic immunosensor with a nanostructured platform based in zinc oxide nanoparticles covered by polyvinyl alcohol (ZnONPs-PVA) coupled to laser-induced fluorescence (LIF) for detection of epithelial cell adhesion molecule (EpCAM). EpCAM is a cell surface protein, and is overexpressed by epithelial carcinomas as such as the lung, colorectal, breast, prostate, head, neck, and hepatic origin. The detection limits (LODs) calculated for our method and for a commercial enzyme-linked immunosorbent assay (ELISA) test kit were 1.2 and 13.9 pg mL-1, respectively. The within- and between-assay variation coefficients for the proposed method were below 6.50%. The results correlated well with those obtained with the commercial ELISA method, thus demonstrating that the new nano-platform integrated into a microfluidic device offers a truthful and useful analytical tool to be easily applied in epithelial cancer diagnosis through EpCAM biomarker determination.
Fil: Fernández Baldo, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Ortega, Francisco G.. Universidad de Granada; España
Fil: Pereira, Sirley Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Bertolino, Franco Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Serrano, María J.. Universidad de Granada; España
Fil: Lorente, José A.. Universidad de Granada; España
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
description In the present work, we report a microfluidic immunosensor with a nanostructured platform based in zinc oxide nanoparticles covered by polyvinyl alcohol (ZnONPs-PVA) coupled to laser-induced fluorescence (LIF) for detection of epithelial cell adhesion molecule (EpCAM). EpCAM is a cell surface protein, and is overexpressed by epithelial carcinomas as such as the lung, colorectal, breast, prostate, head, neck, and hepatic origin. The detection limits (LODs) calculated for our method and for a commercial enzyme-linked immunosorbent assay (ELISA) test kit were 1.2 and 13.9 pg mL-1, respectively. The within- and between-assay variation coefficients for the proposed method were below 6.50%. The results correlated well with those obtained with the commercial ELISA method, thus demonstrating that the new nano-platform integrated into a microfluidic device offers a truthful and useful analytical tool to be easily applied in epithelial cancer diagnosis through EpCAM biomarker determination.
publishDate 2016
dc.date.none.fl_str_mv 2016-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/60584
Fernández Baldo, Martín Alejandro; Ortega, Francisco G.; Pereira, Sirley Vanesa; Bertolino, Franco Adrián; Serrano, María J.; et al.; Nanostructured platform integrated into a microfluidic immunosensor coupled to laser-induced fluorescence for the epithelial cancer biomarker determination; Elsevier Science; Microchemical Journal; 128; 9-2016; 18-25
0026-265X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/60584
identifier_str_mv Fernández Baldo, Martín Alejandro; Ortega, Francisco G.; Pereira, Sirley Vanesa; Bertolino, Franco Adrián; Serrano, María J.; et al.; Nanostructured platform integrated into a microfluidic immunosensor coupled to laser-induced fluorescence for the epithelial cancer biomarker determination; Elsevier Science; Microchemical Journal; 128; 9-2016; 18-25
0026-265X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0026265X16300091
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.microc.2016.03.012
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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