Effect of phytase supplementation of a plant-based diet on phosphorus and nitrogen bioavailability in sea bream Sparus aurata

Autores
Morales, Gabriel Alejandro; Marquez, L.; Saenz de Rodrigañez, M.; Bermúdez, L.; Robles, R.; Moyano, F. J.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
To assess to what extent addition of phytase to a plant-based diet results in spatio-temporal changes of phytate, available P, soluble protein, total amino acids and the activity of the main digestive proteases in gilthead sea bream, fish were fed two plant-based diets with or without phytase. Stomach, proximal intestine and distal intestine contents were monitored for these parameters at 0, 1, 2, 4 and 6 h after feeding. A reduction (P < 0.0001) of the soluble P–IP6 in the stomach when phytase was added to the diet was observed. Within stomach, most of the total P–IP6 was precipitated (86%), possibly due to the low acidification capacity of the sea bream (pH > 4), but 57% of the dietary P–IP6 was dephosphorylated, suggesting that phytase could have the capacity to dephosphorylate insoluble IP6 at such pH. An increment (60%) (P < 0.01) in total gastric protease activity was observed by phytase addition, this being the first demonstration of the in vivo effect of IP6 on the pepsin activity in fish stomach. Gastric pH and residence time of the digesta inside the stomach are critical factors for an efficient phytase action and improve P and N bioavailability in plant-based diets used in fish aquaculture.
Fil: Morales, Gabriel Alejandro. Universidad de Almería; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Marquez, L.. Universidad de Almería; España
Fil: Saenz de Rodrigañez, M.. Universidad de Almería; España
Fil: Bermúdez, L.. Fundación Centro Tecnológico de Acuicultura de Andalucía; España
Fil: Robles, R.. Fundación Centro Tecnológico de Acuicultura de Andalucía; España
Fil: Moyano, F. J.. Universidad de Almería; España
Materia
Amino Acids
Digestive Proteases
Phosphorus
Phytase
Phytate
Sparus Aurata
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/16353
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/16353
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of phytase supplementation of a plant-based diet on phosphorus and nitrogen bioavailability in sea bream Sparus aurataMorales, Gabriel AlejandroMarquez, L.Saenz de Rodrigañez, M.Bermúdez, L.Robles, R.Moyano, F. J.Amino AcidsDigestive ProteasesPhosphorusPhytasePhytateSparus Auratahttps://purl.org/becyt/ford/4.2https://purl.org/becyt/ford/4To assess to what extent addition of phytase to a plant-based diet results in spatio-temporal changes of phytate, available P, soluble protein, total amino acids and the activity of the main digestive proteases in gilthead sea bream, fish were fed two plant-based diets with or without phytase. Stomach, proximal intestine and distal intestine contents were monitored for these parameters at 0, 1, 2, 4 and 6 h after feeding. A reduction (P < 0.0001) of the soluble P–IP6 in the stomach when phytase was added to the diet was observed. Within stomach, most of the total P–IP6 was precipitated (86%), possibly due to the low acidification capacity of the sea bream (pH > 4), but 57% of the dietary P–IP6 was dephosphorylated, suggesting that phytase could have the capacity to dephosphorylate insoluble IP6 at such pH. An increment (60%) (P < 0.01) in total gastric protease activity was observed by phytase addition, this being the first demonstration of the in vivo effect of IP6 on the pepsin activity in fish stomach. Gastric pH and residence time of the digesta inside the stomach are critical factors for an efficient phytase action and improve P and N bioavailability in plant-based diets used in fish aquaculture.Fil: Morales, Gabriel Alejandro. Universidad de Almería; España. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Marquez, L.. Universidad de Almería; EspañaFil: Saenz de Rodrigañez, M.. Universidad de Almería; EspañaFil: Bermúdez, L.. Fundación Centro Tecnológico de Acuicultura de Andalucía; EspañaFil: Robles, R.. Fundación Centro Tecnológico de Acuicultura de Andalucía; EspañaFil: Moyano, F. J.. Universidad de Almería; EspañaWiley2014-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/16353Morales, Gabriel Alejandro; Marquez, L.; Saenz de Rodrigañez, M.; Bermúdez, L.; Robles, R.; et al.; Effect of phytase supplementation of a plant-based diet on phosphorus and nitrogen bioavailability in sea bream Sparus aurata; Wiley; Aquaculture Nutrition; 20; 2; 4-2014; 172-1821353-57731365-2095enginfo:eu-repo/semantics/altIdentifier/doi/10.1111/anu.12063info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/anu.12063/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:20:52Zoai:ri.conicet.gov.ar:11336/16353instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:20:52.937CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of phytase supplementation of a plant-based diet on phosphorus and nitrogen bioavailability in sea bream Sparus aurata
title Effect of phytase supplementation of a plant-based diet on phosphorus and nitrogen bioavailability in sea bream Sparus aurata
spellingShingle Effect of phytase supplementation of a plant-based diet on phosphorus and nitrogen bioavailability in sea bream Sparus aurata
Morales, Gabriel Alejandro
Amino Acids
Digestive Proteases
Phosphorus
Phytase
Phytate
Sparus Aurata
title_short Effect of phytase supplementation of a plant-based diet on phosphorus and nitrogen bioavailability in sea bream Sparus aurata
title_full Effect of phytase supplementation of a plant-based diet on phosphorus and nitrogen bioavailability in sea bream Sparus aurata
title_fullStr Effect of phytase supplementation of a plant-based diet on phosphorus and nitrogen bioavailability in sea bream Sparus aurata
title_full_unstemmed Effect of phytase supplementation of a plant-based diet on phosphorus and nitrogen bioavailability in sea bream Sparus aurata
title_sort Effect of phytase supplementation of a plant-based diet on phosphorus and nitrogen bioavailability in sea bream Sparus aurata
dc.creator.none.fl_str_mv Morales, Gabriel Alejandro
Marquez, L.
Saenz de Rodrigañez, M.
Bermúdez, L.
Robles, R.
Moyano, F. J.
author Morales, Gabriel Alejandro
author_facet Morales, Gabriel Alejandro
Marquez, L.
Saenz de Rodrigañez, M.
Bermúdez, L.
Robles, R.
Moyano, F. J.
author_role author
author2 Marquez, L.
Saenz de Rodrigañez, M.
Bermúdez, L.
Robles, R.
Moyano, F. J.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Amino Acids
Digestive Proteases
Phosphorus
Phytase
Phytate
Sparus Aurata
topic Amino Acids
Digestive Proteases
Phosphorus
Phytase
Phytate
Sparus Aurata
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.2
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv To assess to what extent addition of phytase to a plant-based diet results in spatio-temporal changes of phytate, available P, soluble protein, total amino acids and the activity of the main digestive proteases in gilthead sea bream, fish were fed two plant-based diets with or without phytase. Stomach, proximal intestine and distal intestine contents were monitored for these parameters at 0, 1, 2, 4 and 6 h after feeding. A reduction (P < 0.0001) of the soluble P–IP6 in the stomach when phytase was added to the diet was observed. Within stomach, most of the total P–IP6 was precipitated (86%), possibly due to the low acidification capacity of the sea bream (pH > 4), but 57% of the dietary P–IP6 was dephosphorylated, suggesting that phytase could have the capacity to dephosphorylate insoluble IP6 at such pH. An increment (60%) (P < 0.01) in total gastric protease activity was observed by phytase addition, this being the first demonstration of the in vivo effect of IP6 on the pepsin activity in fish stomach. Gastric pH and residence time of the digesta inside the stomach are critical factors for an efficient phytase action and improve P and N bioavailability in plant-based diets used in fish aquaculture.
Fil: Morales, Gabriel Alejandro. Universidad de Almería; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Marquez, L.. Universidad de Almería; España
Fil: Saenz de Rodrigañez, M.. Universidad de Almería; España
Fil: Bermúdez, L.. Fundación Centro Tecnológico de Acuicultura de Andalucía; España
Fil: Robles, R.. Fundación Centro Tecnológico de Acuicultura de Andalucía; España
Fil: Moyano, F. J.. Universidad de Almería; España
description To assess to what extent addition of phytase to a plant-based diet results in spatio-temporal changes of phytate, available P, soluble protein, total amino acids and the activity of the main digestive proteases in gilthead sea bream, fish were fed two plant-based diets with or without phytase. Stomach, proximal intestine and distal intestine contents were monitored for these parameters at 0, 1, 2, 4 and 6 h after feeding. A reduction (P < 0.0001) of the soluble P–IP6 in the stomach when phytase was added to the diet was observed. Within stomach, most of the total P–IP6 was precipitated (86%), possibly due to the low acidification capacity of the sea bream (pH > 4), but 57% of the dietary P–IP6 was dephosphorylated, suggesting that phytase could have the capacity to dephosphorylate insoluble IP6 at such pH. An increment (60%) (P < 0.01) in total gastric protease activity was observed by phytase addition, this being the first demonstration of the in vivo effect of IP6 on the pepsin activity in fish stomach. Gastric pH and residence time of the digesta inside the stomach are critical factors for an efficient phytase action and improve P and N bioavailability in plant-based diets used in fish aquaculture.
publishDate 2014
dc.date.none.fl_str_mv 2014-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/16353
Morales, Gabriel Alejandro; Marquez, L.; Saenz de Rodrigañez, M.; Bermúdez, L.; Robles, R.; et al.; Effect of phytase supplementation of a plant-based diet on phosphorus and nitrogen bioavailability in sea bream Sparus aurata; Wiley; Aquaculture Nutrition; 20; 2; 4-2014; 172-182
1353-5773
1365-2095
url http://hdl.handle.net/11336/16353
identifier_str_mv Morales, Gabriel Alejandro; Marquez, L.; Saenz de Rodrigañez, M.; Bermúdez, L.; Robles, R.; et al.; Effect of phytase supplementation of a plant-based diet on phosphorus and nitrogen bioavailability in sea bream Sparus aurata; Wiley; Aquaculture Nutrition; 20; 2; 4-2014; 172-182
1353-5773
1365-2095
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/anu.12063
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/anu.12063/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846082590409228288
score 13.22299

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