Adsorption to soils and biochemical characterization of commercial phytases

Autores
Caffaro, Maria Marta; Balestrasse, Karina Beatriz; Rubio, Gerardo
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Commercial phytases are widely used in poultry production, but little is known about their potential use as biofertilizer for agricultural crops as an alternative to reduce the use of synthetic fertilizers. Four purified phytases isolated from Aspergillus Niger and Escherichia coli were characterized biochemically and in terms of their adsorption to soils belonging to the Mollisol order. Three different organic P substrates were used to measure enzyme activity under a wide range of pH (2.3 to 9) and temperatures (-10 to 70 °C) conditions: phytic acid, p-nitrophenyl-phosphate (pNP) and glyceraldehyde-3-phosphate (G3Phosphate). Phytases had a low affinity for the solid phase: 23 %-34 % of the added amount was adsorbed after 1 h of incubation. Phytases from A. Niger showed a higher capacity to release P (13 % on average) than phytases from E. coli. All phytases were active throughout the pH and temperature ranges related to optimum crop production. At pH values commonly found in agricultural soils (5.5-7), A. Niger phytases released P with the following substrate ranking with respect to effectiveness: pNP > phytic acid > G3Phosphate. E. coli phytases, in comparison, released P following with the following substrate ranking with respect to effectiveness: pNP = phytic acid > G3Phosphate. The results obtained are promising in terms of the use of phytases as a complement to P fertilization in agricultural settings and encourage further studies under field conditions.
Fil: Caffaro, Maria Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Ingeniería Agrícola y Uso de la Tierra. Cátedra de Fertilidad y Fertilizantes; Argentina
Fil: Balestrasse, Karina Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Rubio, Gerardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Materia
SOILS
PHYTASES
ADPSORTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/162444
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/162444
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Adsorption to soils and biochemical characterization of commercial phytasesCaffaro, Maria MartaBalestrasse, Karina BeatrizRubio, GerardoSOILSPHYTASESADPSORTIONhttps://purl.org/becyt/ford/4.5https://purl.org/becyt/ford/4Commercial phytases are widely used in poultry production, but little is known about their potential use as biofertilizer for agricultural crops as an alternative to reduce the use of synthetic fertilizers. Four purified phytases isolated from Aspergillus Niger and Escherichia coli were characterized biochemically and in terms of their adsorption to soils belonging to the Mollisol order. Three different organic P substrates were used to measure enzyme activity under a wide range of pH (2.3 to 9) and temperatures (-10 to 70 °C) conditions: phytic acid, p-nitrophenyl-phosphate (pNP) and glyceraldehyde-3-phosphate (G3Phosphate). Phytases had a low affinity for the solid phase: 23 %-34 % of the added amount was adsorbed after 1 h of incubation. Phytases from A. Niger showed a higher capacity to release P (13 % on average) than phytases from E. coli. All phytases were active throughout the pH and temperature ranges related to optimum crop production. At pH values commonly found in agricultural soils (5.5-7), A. Niger phytases released P with the following substrate ranking with respect to effectiveness: pNP > phytic acid > G3Phosphate. E. coli phytases, in comparison, released P following with the following substrate ranking with respect to effectiveness: pNP = phytic acid > G3Phosphate. The results obtained are promising in terms of the use of phytases as a complement to P fertilization in agricultural settings and encourage further studies under field conditions.Fil: Caffaro, Maria Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Ingeniería Agrícola y Uso de la Tierra. Cátedra de Fertilidad y Fertilizantes; ArgentinaFil: Balestrasse, Karina Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina. Universidad de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Rubio, Gerardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; ArgentinaCopernicus Publications2020-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/162444Caffaro, Maria Marta; Balestrasse, Karina Beatriz; Rubio, Gerardo; Adsorption to soils and biochemical characterization of commercial phytases; Copernicus Publications; Soil; 6; 1; 4-2020; 153-1622199-398XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://soil.copernicus.org/articles/6/153/2020/info:eu-repo/semantics/altIdentifier/doi/10.5194/soil-6-153-2020info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:01:27Zoai:ri.conicet.gov.ar:11336/162444instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:01:27.665CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Adsorption to soils and biochemical characterization of commercial phytases
title Adsorption to soils and biochemical characterization of commercial phytases
spellingShingle Adsorption to soils and biochemical characterization of commercial phytases
Caffaro, Maria Marta
SOILS
PHYTASES
ADPSORTION
title_short Adsorption to soils and biochemical characterization of commercial phytases
title_full Adsorption to soils and biochemical characterization of commercial phytases
title_fullStr Adsorption to soils and biochemical characterization of commercial phytases
title_full_unstemmed Adsorption to soils and biochemical characterization of commercial phytases
title_sort Adsorption to soils and biochemical characterization of commercial phytases
dc.creator.none.fl_str_mv Caffaro, Maria Marta
Balestrasse, Karina Beatriz
Rubio, Gerardo
author Caffaro, Maria Marta
author_facet Caffaro, Maria Marta
Balestrasse, Karina Beatriz
Rubio, Gerardo
author_role author
author2 Balestrasse, Karina Beatriz
Rubio, Gerardo
author2_role author
author
dc.subject.none.fl_str_mv SOILS
PHYTASES
ADPSORTION
topic SOILS
PHYTASES
ADPSORTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.5
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Commercial phytases are widely used in poultry production, but little is known about their potential use as biofertilizer for agricultural crops as an alternative to reduce the use of synthetic fertilizers. Four purified phytases isolated from Aspergillus Niger and Escherichia coli were characterized biochemically and in terms of their adsorption to soils belonging to the Mollisol order. Three different organic P substrates were used to measure enzyme activity under a wide range of pH (2.3 to 9) and temperatures (-10 to 70 °C) conditions: phytic acid, p-nitrophenyl-phosphate (pNP) and glyceraldehyde-3-phosphate (G3Phosphate). Phytases had a low affinity for the solid phase: 23 %-34 % of the added amount was adsorbed after 1 h of incubation. Phytases from A. Niger showed a higher capacity to release P (13 % on average) than phytases from E. coli. All phytases were active throughout the pH and temperature ranges related to optimum crop production. At pH values commonly found in agricultural soils (5.5-7), A. Niger phytases released P with the following substrate ranking with respect to effectiveness: pNP > phytic acid > G3Phosphate. E. coli phytases, in comparison, released P following with the following substrate ranking with respect to effectiveness: pNP = phytic acid > G3Phosphate. The results obtained are promising in terms of the use of phytases as a complement to P fertilization in agricultural settings and encourage further studies under field conditions.
Fil: Caffaro, Maria Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Ingeniería Agrícola y Uso de la Tierra. Cátedra de Fertilidad y Fertilizantes; Argentina
Fil: Balestrasse, Karina Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Rubio, Gerardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
description Commercial phytases are widely used in poultry production, but little is known about their potential use as biofertilizer for agricultural crops as an alternative to reduce the use of synthetic fertilizers. Four purified phytases isolated from Aspergillus Niger and Escherichia coli were characterized biochemically and in terms of their adsorption to soils belonging to the Mollisol order. Three different organic P substrates were used to measure enzyme activity under a wide range of pH (2.3 to 9) and temperatures (-10 to 70 °C) conditions: phytic acid, p-nitrophenyl-phosphate (pNP) and glyceraldehyde-3-phosphate (G3Phosphate). Phytases had a low affinity for the solid phase: 23 %-34 % of the added amount was adsorbed after 1 h of incubation. Phytases from A. Niger showed a higher capacity to release P (13 % on average) than phytases from E. coli. All phytases were active throughout the pH and temperature ranges related to optimum crop production. At pH values commonly found in agricultural soils (5.5-7), A. Niger phytases released P with the following substrate ranking with respect to effectiveness: pNP > phytic acid > G3Phosphate. E. coli phytases, in comparison, released P following with the following substrate ranking with respect to effectiveness: pNP = phytic acid > G3Phosphate. The results obtained are promising in terms of the use of phytases as a complement to P fertilization in agricultural settings and encourage further studies under field conditions.
publishDate 2020
dc.date.none.fl_str_mv 2020-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/162444
Caffaro, Maria Marta; Balestrasse, Karina Beatriz; Rubio, Gerardo; Adsorption to soils and biochemical characterization of commercial phytases; Copernicus Publications; Soil; 6; 1; 4-2020; 153-162
2199-398X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/162444
identifier_str_mv Caffaro, Maria Marta; Balestrasse, Karina Beatriz; Rubio, Gerardo; Adsorption to soils and biochemical characterization of commercial phytases; Copernicus Publications; Soil; 6; 1; 4-2020; 153-162
2199-398X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://soil.copernicus.org/articles/6/153/2020/
info:eu-repo/semantics/altIdentifier/doi/10.5194/soil-6-153-2020
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Copernicus Publications
publisher.none.fl_str_mv Copernicus Publications
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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