Biological control of Alphitobius diaperinus with Steinernema rarum CUL and Heterorhabditis bacteriophora SMC and feasibility of application in rice hull

Autores
del Valle, Eleodoro Eduardo; Frizzo, Laureano Sebastian; Malmierca, Melisa; Zbrun, María Virginia; Lax, Paola; Doucet, Marcelo Edmundo
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Chemical insecticides often are not efficient in reducing Alphitobius diaperinus populations in poultry farms and have induced insect resistance. Entomopathogenic nematodes (EPNs) appear as an alternative to pest biocontrol agents; however, their efficiency on rice hull litter is still unknown. The aim of this study was to evaluate the potential of Steinernema rarum (CUL isolate), Heterorhabditis bacteriophora (SMC isolate) and their symbiotic bacteria as biocontrol agents of A. diaperinus larvae and adults in rice hull litter. Mortality of insect adults and fifth- and seventh-instar larvae was determined in Petri dishes containing filter paper or rice hull. The effect of Xenorhabdus szentirmaii and Photorhabdus luminescens on L7 and adults in rice hull substrate was also evaluated. Moreover, the effectiveness of the EPNs was assessed in bags in an environment simulating the prevailing conditions in a broiler chicken farm. Under laboratory conditions, insect mortality was significantly affected by EPN species, substrate, insect stage and time after application. Mortality of L5 and L7 caused by EPNs in rice hulls reached a peak of 64 and 57.3 %, respectively, whereas symbiotic bacteria applied on rice hull showed low mortality rate at both insect stages. In the simulated poultry farm conditions, S. rarum and H. bacteriophora caused up to 40.8 and 50.4 % mortality of A. diaperinus larvae, respectively, but with adult mortality being low for both species. We conclude that infective juveniles from S. rarum CUL and H. bacteriophora SMC isolates can be used as biological control agents of A. diaperinus larvae in chicken litter containing rice hulls.
Fil: del Valle, Eleodoro Eduardo. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Frizzo, Laureano Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Malmierca, Melisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Zbrun, María Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Lax, Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Centro de Zoología Aplicada; Argentina
Fil: Doucet, Marcelo Edmundo. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Centro de Zoología Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina
Materia
Alphitobius Diaperinus
Biological Control
Entomopathogenic Nematode
Poultry Production
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/39630
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/39630
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Biological control of Alphitobius diaperinus with Steinernema rarum CUL and Heterorhabditis bacteriophora SMC and feasibility of application in rice hulldel Valle, Eleodoro EduardoFrizzo, Laureano SebastianMalmierca, MelisaZbrun, María VirginiaLax, PaolaDoucet, Marcelo EdmundoAlphitobius DiaperinusBiological ControlEntomopathogenic NematodePoultry Productionhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Chemical insecticides often are not efficient in reducing Alphitobius diaperinus populations in poultry farms and have induced insect resistance. Entomopathogenic nematodes (EPNs) appear as an alternative to pest biocontrol agents; however, their efficiency on rice hull litter is still unknown. The aim of this study was to evaluate the potential of Steinernema rarum (CUL isolate), Heterorhabditis bacteriophora (SMC isolate) and their symbiotic bacteria as biocontrol agents of A. diaperinus larvae and adults in rice hull litter. Mortality of insect adults and fifth- and seventh-instar larvae was determined in Petri dishes containing filter paper or rice hull. The effect of Xenorhabdus szentirmaii and Photorhabdus luminescens on L7 and adults in rice hull substrate was also evaluated. Moreover, the effectiveness of the EPNs was assessed in bags in an environment simulating the prevailing conditions in a broiler chicken farm. Under laboratory conditions, insect mortality was significantly affected by EPN species, substrate, insect stage and time after application. Mortality of L5 and L7 caused by EPNs in rice hulls reached a peak of 64 and 57.3 %, respectively, whereas symbiotic bacteria applied on rice hull showed low mortality rate at both insect stages. In the simulated poultry farm conditions, S. rarum and H. bacteriophora caused up to 40.8 and 50.4 % mortality of A. diaperinus larvae, respectively, but with adult mortality being low for both species. We conclude that infective juveniles from S. rarum CUL and H. bacteriophora SMC isolates can be used as biological control agents of A. diaperinus larvae in chicken litter containing rice hulls.Fil: del Valle, Eleodoro Eduardo. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Frizzo, Laureano Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Malmierca, Melisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Zbrun, María Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Lax, Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Centro de Zoología Aplicada; ArgentinaFil: Doucet, Marcelo Edmundo. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Centro de Zoología Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; ArgentinaSpringer Heidelberg2016-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/39630del Valle, Eleodoro Eduardo; Frizzo, Laureano Sebastian; Malmierca, Melisa; Zbrun, María Virginia; Lax, Paola; et al.; Biological control of Alphitobius diaperinus with Steinernema rarum CUL and Heterorhabditis bacteriophora SMC and feasibility of application in rice hull; Springer Heidelberg; Journal of Pest Science; 89; 1; 3-2016; 161-1701612-4758CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s10340-015-0669-8info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10340-015-0669-8info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:45:00Zoai:ri.conicet.gov.ar:11336/39630instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:45:00.454CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Biological control of Alphitobius diaperinus with Steinernema rarum CUL and Heterorhabditis bacteriophora SMC and feasibility of application in rice hull
title Biological control of Alphitobius diaperinus with Steinernema rarum CUL and Heterorhabditis bacteriophora SMC and feasibility of application in rice hull
spellingShingle Biological control of Alphitobius diaperinus with Steinernema rarum CUL and Heterorhabditis bacteriophora SMC and feasibility of application in rice hull
del Valle, Eleodoro Eduardo
Alphitobius Diaperinus
Biological Control
Entomopathogenic Nematode
Poultry Production
title_short Biological control of Alphitobius diaperinus with Steinernema rarum CUL and Heterorhabditis bacteriophora SMC and feasibility of application in rice hull
title_full Biological control of Alphitobius diaperinus with Steinernema rarum CUL and Heterorhabditis bacteriophora SMC and feasibility of application in rice hull
title_fullStr Biological control of Alphitobius diaperinus with Steinernema rarum CUL and Heterorhabditis bacteriophora SMC and feasibility of application in rice hull
title_full_unstemmed Biological control of Alphitobius diaperinus with Steinernema rarum CUL and Heterorhabditis bacteriophora SMC and feasibility of application in rice hull
title_sort Biological control of Alphitobius diaperinus with Steinernema rarum CUL and Heterorhabditis bacteriophora SMC and feasibility of application in rice hull
dc.creator.none.fl_str_mv del Valle, Eleodoro Eduardo
Frizzo, Laureano Sebastian
Malmierca, Melisa
Zbrun, María Virginia
Lax, Paola
Doucet, Marcelo Edmundo
author del Valle, Eleodoro Eduardo
author_facet del Valle, Eleodoro Eduardo
Frizzo, Laureano Sebastian
Malmierca, Melisa
Zbrun, María Virginia
Lax, Paola
Doucet, Marcelo Edmundo
author_role author
author2 Frizzo, Laureano Sebastian
Malmierca, Melisa
Zbrun, María Virginia
Lax, Paola
Doucet, Marcelo Edmundo
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Alphitobius Diaperinus
Biological Control
Entomopathogenic Nematode
Poultry Production
topic Alphitobius Diaperinus
Biological Control
Entomopathogenic Nematode
Poultry Production
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Chemical insecticides often are not efficient in reducing Alphitobius diaperinus populations in poultry farms and have induced insect resistance. Entomopathogenic nematodes (EPNs) appear as an alternative to pest biocontrol agents; however, their efficiency on rice hull litter is still unknown. The aim of this study was to evaluate the potential of Steinernema rarum (CUL isolate), Heterorhabditis bacteriophora (SMC isolate) and their symbiotic bacteria as biocontrol agents of A. diaperinus larvae and adults in rice hull litter. Mortality of insect adults and fifth- and seventh-instar larvae was determined in Petri dishes containing filter paper or rice hull. The effect of Xenorhabdus szentirmaii and Photorhabdus luminescens on L7 and adults in rice hull substrate was also evaluated. Moreover, the effectiveness of the EPNs was assessed in bags in an environment simulating the prevailing conditions in a broiler chicken farm. Under laboratory conditions, insect mortality was significantly affected by EPN species, substrate, insect stage and time after application. Mortality of L5 and L7 caused by EPNs in rice hulls reached a peak of 64 and 57.3 %, respectively, whereas symbiotic bacteria applied on rice hull showed low mortality rate at both insect stages. In the simulated poultry farm conditions, S. rarum and H. bacteriophora caused up to 40.8 and 50.4 % mortality of A. diaperinus larvae, respectively, but with adult mortality being low for both species. We conclude that infective juveniles from S. rarum CUL and H. bacteriophora SMC isolates can be used as biological control agents of A. diaperinus larvae in chicken litter containing rice hulls.
Fil: del Valle, Eleodoro Eduardo. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Frizzo, Laureano Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Malmierca, Melisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Zbrun, María Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Lax, Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Centro de Zoología Aplicada; Argentina
Fil: Doucet, Marcelo Edmundo. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Centro de Zoología Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina
description Chemical insecticides often are not efficient in reducing Alphitobius diaperinus populations in poultry farms and have induced insect resistance. Entomopathogenic nematodes (EPNs) appear as an alternative to pest biocontrol agents; however, their efficiency on rice hull litter is still unknown. The aim of this study was to evaluate the potential of Steinernema rarum (CUL isolate), Heterorhabditis bacteriophora (SMC isolate) and their symbiotic bacteria as biocontrol agents of A. diaperinus larvae and adults in rice hull litter. Mortality of insect adults and fifth- and seventh-instar larvae was determined in Petri dishes containing filter paper or rice hull. The effect of Xenorhabdus szentirmaii and Photorhabdus luminescens on L7 and adults in rice hull substrate was also evaluated. Moreover, the effectiveness of the EPNs was assessed in bags in an environment simulating the prevailing conditions in a broiler chicken farm. Under laboratory conditions, insect mortality was significantly affected by EPN species, substrate, insect stage and time after application. Mortality of L5 and L7 caused by EPNs in rice hulls reached a peak of 64 and 57.3 %, respectively, whereas symbiotic bacteria applied on rice hull showed low mortality rate at both insect stages. In the simulated poultry farm conditions, S. rarum and H. bacteriophora caused up to 40.8 and 50.4 % mortality of A. diaperinus larvae, respectively, but with adult mortality being low for both species. We conclude that infective juveniles from S. rarum CUL and H. bacteriophora SMC isolates can be used as biological control agents of A. diaperinus larvae in chicken litter containing rice hulls.
publishDate 2016
dc.date.none.fl_str_mv 2016-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/39630
del Valle, Eleodoro Eduardo; Frizzo, Laureano Sebastian; Malmierca, Melisa; Zbrun, María Virginia; Lax, Paola; et al.; Biological control of Alphitobius diaperinus with Steinernema rarum CUL and Heterorhabditis bacteriophora SMC and feasibility of application in rice hull; Springer Heidelberg; Journal of Pest Science; 89; 1; 3-2016; 161-170
1612-4758
CONICET Digital
CONICET
url http://hdl.handle.net/11336/39630
identifier_str_mv del Valle, Eleodoro Eduardo; Frizzo, Laureano Sebastian; Malmierca, Melisa; Zbrun, María Virginia; Lax, Paola; et al.; Biological control of Alphitobius diaperinus with Steinernema rarum CUL and Heterorhabditis bacteriophora SMC and feasibility of application in rice hull; Springer Heidelberg; Journal of Pest Science; 89; 1; 3-2016; 161-170
1612-4758
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s10340-015-0669-8
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10340-015-0669-8
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer Heidelberg
publisher.none.fl_str_mv Springer Heidelberg
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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