Sublethal effects of an Argentine Bacillus thuringiensis strain on the development and fitness of Alphitobius diaperinus (Coleoptera: Tenebrionidae)

Autores
Antonuccio, Gisele Ivone; Sauka, Diego Hernan
Año de publicación
2025
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Poster y resumen
Alphitobius diaperinus, the lesser mealworm, is a key pest of poultry in broiler and layer facilities. The highly virulent INTA Mo4-4 strain of Bacillus thuringiensis is the most effective choice for controlling larval A. diaperinus. This study evaluated its sublethal effects on development, survival and fitness. Initially, six concentrations of INTA Mo4-4 spores and crystals were incorporated into the diet of A. diaperinus starting with 4-day-old larvae to assess virulence. Mortality was recorded after 14 d at 28 °C, and LC30 and LC50 were determined from four independent bioassays, yielding values of 69 and 136 µg/ml, respectively. Subsequently, sublethal bioassays using the LC30 and LC50, alongside a control group, determined the effects on surviving larvae. After 14 d at 28 °C, 791 larvae from five independent bioassays were analyzed. Their weights and larval areas were measured and later monitored to determine the durations of their larval and pupal stages, as well as their pupal and adult weights and body areas. Sublethal effects were clear in the LC30 and LC50 treatments, showing significantly reduced weights/areas per larva (0.25 mg/0.97 mm² and 0.20 mg/0.82 mm², respectively) compared to controls (0.37 mg/1.32 mm²). Although these trends persisted, differences were no longer statistically significant for developmental periods, pupae or adult fitness traits. Average larval (76.7, 83.1 and 104.6 days) and pupal periods (5.7, 6.4 and 7.0 days) and pupal (10.08 mg/9.32 mm², 8.75 mg/8.44 mm² and 8.12 mg/7.80 mm²) and adult weights/areas (8.97 mg/9.63 mm²; 7.65 mg/8.54 mm²; and 6.96 mg/7.95 mm²) per specimen followed similar trends across the control, LC30, and LC50 treatments, respectively. This study showed that INTA Mo4-4 induces both mortality and sublethal effects that could affect the development and fitness of A. diaperinus, offering valuable insights for bioinsecticide development targeting this pest.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Antonuccio, Gisele. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA); Argentina
Fil: Antonuccio, Gisele. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Sauka, Diego Herman. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
The 2nd International Electronic Conference on Entomology, 19 al 21 de mayo del 2025, en linea
Materia
Bacillus thuringiensis
Alphitobius diaperinus
Efecto Subletal
Argentina
Control Biológico
Sublethal Effects
Biological Control
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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oai_identifier_str oai:localhost:20.500.12123/25812
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spelling Sublethal effects of an Argentine Bacillus thuringiensis strain on the development and fitness of Alphitobius diaperinus (Coleoptera: Tenebrionidae)Antonuccio, Gisele IvoneSauka, Diego HernanBacillus thuringiensisAlphitobius diaperinusEfecto SubletalArgentinaControl BiológicoSublethal EffectsBiological ControlPoster y resumenAlphitobius diaperinus, the lesser mealworm, is a key pest of poultry in broiler and layer facilities. The highly virulent INTA Mo4-4 strain of Bacillus thuringiensis is the most effective choice for controlling larval A. diaperinus. This study evaluated its sublethal effects on development, survival and fitness. Initially, six concentrations of INTA Mo4-4 spores and crystals were incorporated into the diet of A. diaperinus starting with 4-day-old larvae to assess virulence. Mortality was recorded after 14 d at 28 °C, and LC30 and LC50 were determined from four independent bioassays, yielding values of 69 and 136 µg/ml, respectively. Subsequently, sublethal bioassays using the LC30 and LC50, alongside a control group, determined the effects on surviving larvae. After 14 d at 28 °C, 791 larvae from five independent bioassays were analyzed. Their weights and larval areas were measured and later monitored to determine the durations of their larval and pupal stages, as well as their pupal and adult weights and body areas. Sublethal effects were clear in the LC30 and LC50 treatments, showing significantly reduced weights/areas per larva (0.25 mg/0.97 mm² and 0.20 mg/0.82 mm², respectively) compared to controls (0.37 mg/1.32 mm²). Although these trends persisted, differences were no longer statistically significant for developmental periods, pupae or adult fitness traits. Average larval (76.7, 83.1 and 104.6 days) and pupal periods (5.7, 6.4 and 7.0 days) and pupal (10.08 mg/9.32 mm², 8.75 mg/8.44 mm² and 8.12 mg/7.80 mm²) and adult weights/areas (8.97 mg/9.63 mm²; 7.65 mg/8.54 mm²; and 6.96 mg/7.95 mm²) per specimen followed similar trends across the control, LC30, and LC50 treatments, respectively. This study showed that INTA Mo4-4 induces both mortality and sublethal effects that could affect the development and fitness of A. diaperinus, offering valuable insights for bioinsecticide development targeting this pest.Instituto de Microbiología y Zoología Agrícola (IMYZA)Fil: Antonuccio, Gisele. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA); ArgentinaFil: Antonuccio, Gisele. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); ArgentinaFil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); ArgentinaFil: Sauka, Diego Herman. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaMDPI2026-04-15T12:21:04Z2026-04-15T12:21:04Z2025-05-19info:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfhttp://hdl.handle.net/20.500.12123/25812https://sciforum.net/paper/view/22707The 2nd International Electronic Conference on Entomology, 19 al 21 de mayo del 2025, en lineareponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-04-16T09:53:31Zoai:localhost:20.500.12123/25812instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-04-16 09:53:32.13INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Sublethal effects of an Argentine Bacillus thuringiensis strain on the development and fitness of Alphitobius diaperinus (Coleoptera: Tenebrionidae)
title Sublethal effects of an Argentine Bacillus thuringiensis strain on the development and fitness of Alphitobius diaperinus (Coleoptera: Tenebrionidae)
spellingShingle Sublethal effects of an Argentine Bacillus thuringiensis strain on the development and fitness of Alphitobius diaperinus (Coleoptera: Tenebrionidae)
Antonuccio, Gisele Ivone
Bacillus thuringiensis
Alphitobius diaperinus
Efecto Subletal
Argentina
Control Biológico
Sublethal Effects
Biological Control
title_short Sublethal effects of an Argentine Bacillus thuringiensis strain on the development and fitness of Alphitobius diaperinus (Coleoptera: Tenebrionidae)
title_full Sublethal effects of an Argentine Bacillus thuringiensis strain on the development and fitness of Alphitobius diaperinus (Coleoptera: Tenebrionidae)
title_fullStr Sublethal effects of an Argentine Bacillus thuringiensis strain on the development and fitness of Alphitobius diaperinus (Coleoptera: Tenebrionidae)
title_full_unstemmed Sublethal effects of an Argentine Bacillus thuringiensis strain on the development and fitness of Alphitobius diaperinus (Coleoptera: Tenebrionidae)
title_sort Sublethal effects of an Argentine Bacillus thuringiensis strain on the development and fitness of Alphitobius diaperinus (Coleoptera: Tenebrionidae)
dc.creator.none.fl_str_mv Antonuccio, Gisele Ivone
Sauka, Diego Hernan
author Antonuccio, Gisele Ivone
author_facet Antonuccio, Gisele Ivone
Sauka, Diego Hernan
author_role author
author2 Sauka, Diego Hernan
author2_role author
dc.subject.none.fl_str_mv Bacillus thuringiensis
Alphitobius diaperinus
Efecto Subletal
Argentina
Control Biológico
Sublethal Effects
Biological Control
topic Bacillus thuringiensis
Alphitobius diaperinus
Efecto Subletal
Argentina
Control Biológico
Sublethal Effects
Biological Control
dc.description.none.fl_txt_mv Poster y resumen
Alphitobius diaperinus, the lesser mealworm, is a key pest of poultry in broiler and layer facilities. The highly virulent INTA Mo4-4 strain of Bacillus thuringiensis is the most effective choice for controlling larval A. diaperinus. This study evaluated its sublethal effects on development, survival and fitness. Initially, six concentrations of INTA Mo4-4 spores and crystals were incorporated into the diet of A. diaperinus starting with 4-day-old larvae to assess virulence. Mortality was recorded after 14 d at 28 °C, and LC30 and LC50 were determined from four independent bioassays, yielding values of 69 and 136 µg/ml, respectively. Subsequently, sublethal bioassays using the LC30 and LC50, alongside a control group, determined the effects on surviving larvae. After 14 d at 28 °C, 791 larvae from five independent bioassays were analyzed. Their weights and larval areas were measured and later monitored to determine the durations of their larval and pupal stages, as well as their pupal and adult weights and body areas. Sublethal effects were clear in the LC30 and LC50 treatments, showing significantly reduced weights/areas per larva (0.25 mg/0.97 mm² and 0.20 mg/0.82 mm², respectively) compared to controls (0.37 mg/1.32 mm²). Although these trends persisted, differences were no longer statistically significant for developmental periods, pupae or adult fitness traits. Average larval (76.7, 83.1 and 104.6 days) and pupal periods (5.7, 6.4 and 7.0 days) and pupal (10.08 mg/9.32 mm², 8.75 mg/8.44 mm² and 8.12 mg/7.80 mm²) and adult weights/areas (8.97 mg/9.63 mm²; 7.65 mg/8.54 mm²; and 6.96 mg/7.95 mm²) per specimen followed similar trends across the control, LC30, and LC50 treatments, respectively. This study showed that INTA Mo4-4 induces both mortality and sublethal effects that could affect the development and fitness of A. diaperinus, offering valuable insights for bioinsecticide development targeting this pest.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Antonuccio, Gisele. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA); Argentina
Fil: Antonuccio, Gisele. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Sauka, Diego Herman. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Poster y resumen
publishDate 2025
dc.date.none.fl_str_mv 2025-05-19
2026-04-15T12:21:04Z
2026-04-15T12:21:04Z
dc.type.none.fl_str_mv info:eu-repo/semantics/conferenceObject
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
format conferenceObject
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/25812
https://sciforum.net/paper/view/22707
url http://hdl.handle.net/20.500.12123/25812
https://sciforum.net/paper/view/22707
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv The 2nd International Electronic Conference on Entomology, 19 al 21 de mayo del 2025, en linea
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 13.203462

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