Isolation and identification of culturable gut microbiota in the larval stage of lesser mealworm (Alphitobius diaperinus)

Autores
Antonuccio, Gisele Ivonne; Sauka, Diego Hernan
Año de publicación
2023
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Poster y artículo
The highly prevalent pest Alphitobius diaperinus (Coleoptera: Tenebrionidae) causes significant structural damage in poultry farms. Despite previous investigations on its carriage of pathogenic microorganisms, our understanding of its microbiome remains limited. This study aimed to analyze the diversity of culturable gut microbiota in A. diaperinus obtained from laboratory breeding. Fifteen seventh instar larvae underwent a 24-hour starvation period, followed by surface disinfection. Dissected midguts were homogenized and plated on nutrient agar (NA), brain heart infusion agar (BHI), and Bacillus cereus agar (BC). The cultured isolates were subjected to gram staining, phylogenetic analysis, biochemical property evaluation, and metabolic activity assessment. Bacterial counts were higher in BHI (2.51x105 CFU/gut) than in NA (2.25x105 CFU/gut), possibly due to nutrient richness. NA exhibited a dominant colony morphology of gram-negative bacilli, while BHI displayed additional distinct colonies of gram-positive cocci. Surprisingly, yeast-like colonies were observed on BC plates. Based on 16S rRNA gene sequences, eight bacterial isolates were identified as Enterobacter sp., and two as Staphylococcus sp. Using RNA gene ITS region sequences, two yeast isolates were identified as Debaryomyces sp. and Hyphopichia sp. A preliminary species-level identification of bacteria (Enterobacter cloacae, Staphylococcus gallinarum, and Staphylococcus succinus) was achieved using API systems and complementary biochemical tests. Discrepancies between phylogenetic analysis and phenotypic data suggest the potential existence of new species or subspecies. Further comprehensive studies are required to confirm this hypothesis.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Antonuccio, Gisele Ivonne. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Antonuccio, Gisele Ivonne. Servicio Nacional de Sanidad y Calidad Agroalimentaria; Argentina
Fil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Sauka, Diego Herman. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
2nd International Electronic Conference on Microbiology (Noviembre 2023)
Materia
Control Biológico
Biological Control
Yellow Mealworm
Microbial Flora
Gusano de la Harina
Alphitobius diaperinus
Flora Microbiana
Microbiota
Culturable Microorganisms
Bacterial Diversity
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/16587
Acceder
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spelling Isolation and identification of culturable gut microbiota in the larval stage of lesser mealworm (Alphitobius diaperinus)Antonuccio, Gisele IvonneSauka, Diego HernanControl BiológicoBiological ControlYellow MealwormMicrobial FloraGusano de la HarinaAlphitobius diaperinusFlora MicrobianaMicrobiotaCulturable MicroorganismsBacterial DiversityPoster y artículoThe highly prevalent pest Alphitobius diaperinus (Coleoptera: Tenebrionidae) causes significant structural damage in poultry farms. Despite previous investigations on its carriage of pathogenic microorganisms, our understanding of its microbiome remains limited. This study aimed to analyze the diversity of culturable gut microbiota in A. diaperinus obtained from laboratory breeding. Fifteen seventh instar larvae underwent a 24-hour starvation period, followed by surface disinfection. Dissected midguts were homogenized and plated on nutrient agar (NA), brain heart infusion agar (BHI), and Bacillus cereus agar (BC). The cultured isolates were subjected to gram staining, phylogenetic analysis, biochemical property evaluation, and metabolic activity assessment. Bacterial counts were higher in BHI (2.51x105 CFU/gut) than in NA (2.25x105 CFU/gut), possibly due to nutrient richness. NA exhibited a dominant colony morphology of gram-negative bacilli, while BHI displayed additional distinct colonies of gram-positive cocci. Surprisingly, yeast-like colonies were observed on BC plates. Based on 16S rRNA gene sequences, eight bacterial isolates were identified as Enterobacter sp., and two as Staphylococcus sp. Using RNA gene ITS region sequences, two yeast isolates were identified as Debaryomyces sp. and Hyphopichia sp. A preliminary species-level identification of bacteria (Enterobacter cloacae, Staphylococcus gallinarum, and Staphylococcus succinus) was achieved using API systems and complementary biochemical tests. Discrepancies between phylogenetic analysis and phenotypic data suggest the potential existence of new species or subspecies. Further comprehensive studies are required to confirm this hypothesis.Instituto de Microbiología y Zoología Agrícola (IMYZA)Fil: Antonuccio, Gisele Ivonne. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Antonuccio, Gisele Ivonne. Servicio Nacional de Sanidad y Calidad Agroalimentaria; ArgentinaFil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Sauka, Diego Herman. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaSciforum2024-02-14T10:46:22Z2024-02-14T10:46:22Z2023-11-30info:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfhttp://hdl.handle.net/20.500.12123/16587https://sciforum.net/paper/view/164652nd International Electronic Conference on Microbiology (Noviembre 2023)2nd International Electronic Conference on Microbiology (Noviembre 2023)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-23T11:18:40Zoai:localhost:20.500.12123/16587instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:18:40.538INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Isolation and identification of culturable gut microbiota in the larval stage of lesser mealworm (Alphitobius diaperinus)
title Isolation and identification of culturable gut microbiota in the larval stage of lesser mealworm (Alphitobius diaperinus)
spellingShingle Isolation and identification of culturable gut microbiota in the larval stage of lesser mealworm (Alphitobius diaperinus)
Antonuccio, Gisele Ivonne
Control Biológico
Biological Control
Yellow Mealworm
Microbial Flora
Gusano de la Harina
Alphitobius diaperinus
Flora Microbiana
Microbiota
Culturable Microorganisms
Bacterial Diversity
title_short Isolation and identification of culturable gut microbiota in the larval stage of lesser mealworm (Alphitobius diaperinus)
title_full Isolation and identification of culturable gut microbiota in the larval stage of lesser mealworm (Alphitobius diaperinus)
title_fullStr Isolation and identification of culturable gut microbiota in the larval stage of lesser mealworm (Alphitobius diaperinus)
title_full_unstemmed Isolation and identification of culturable gut microbiota in the larval stage of lesser mealworm (Alphitobius diaperinus)
title_sort Isolation and identification of culturable gut microbiota in the larval stage of lesser mealworm (Alphitobius diaperinus)
dc.creator.none.fl_str_mv Antonuccio, Gisele Ivonne
Sauka, Diego Hernan
author Antonuccio, Gisele Ivonne
author_facet Antonuccio, Gisele Ivonne
Sauka, Diego Hernan
author_role author
author2 Sauka, Diego Hernan
author2_role author
dc.subject.none.fl_str_mv Control Biológico
Biological Control
Yellow Mealworm
Microbial Flora
Gusano de la Harina
Alphitobius diaperinus
Flora Microbiana
Microbiota
Culturable Microorganisms
Bacterial Diversity
topic Control Biológico
Biological Control
Yellow Mealworm
Microbial Flora
Gusano de la Harina
Alphitobius diaperinus
Flora Microbiana
Microbiota
Culturable Microorganisms
Bacterial Diversity
dc.description.none.fl_txt_mv Poster y artículo
The highly prevalent pest Alphitobius diaperinus (Coleoptera: Tenebrionidae) causes significant structural damage in poultry farms. Despite previous investigations on its carriage of pathogenic microorganisms, our understanding of its microbiome remains limited. This study aimed to analyze the diversity of culturable gut microbiota in A. diaperinus obtained from laboratory breeding. Fifteen seventh instar larvae underwent a 24-hour starvation period, followed by surface disinfection. Dissected midguts were homogenized and plated on nutrient agar (NA), brain heart infusion agar (BHI), and Bacillus cereus agar (BC). The cultured isolates were subjected to gram staining, phylogenetic analysis, biochemical property evaluation, and metabolic activity assessment. Bacterial counts were higher in BHI (2.51x105 CFU/gut) than in NA (2.25x105 CFU/gut), possibly due to nutrient richness. NA exhibited a dominant colony morphology of gram-negative bacilli, while BHI displayed additional distinct colonies of gram-positive cocci. Surprisingly, yeast-like colonies were observed on BC plates. Based on 16S rRNA gene sequences, eight bacterial isolates were identified as Enterobacter sp., and two as Staphylococcus sp. Using RNA gene ITS region sequences, two yeast isolates were identified as Debaryomyces sp. and Hyphopichia sp. A preliminary species-level identification of bacteria (Enterobacter cloacae, Staphylococcus gallinarum, and Staphylococcus succinus) was achieved using API systems and complementary biochemical tests. Discrepancies between phylogenetic analysis and phenotypic data suggest the potential existence of new species or subspecies. Further comprehensive studies are required to confirm this hypothesis.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Antonuccio, Gisele Ivonne. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Antonuccio, Gisele Ivonne. Servicio Nacional de Sanidad y Calidad Agroalimentaria; Argentina
Fil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Sauka, Diego Herman. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Poster y artículo
publishDate 2023
dc.date.none.fl_str_mv 2023-11-30
2024-02-14T10:46:22Z
2024-02-14T10:46:22Z
dc.type.none.fl_str_mv info:eu-repo/semantics/conferenceObject
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
format conferenceObject
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/16587
https://sciforum.net/paper/view/16465
2nd International Electronic Conference on Microbiology (Noviembre 2023)
url http://hdl.handle.net/20.500.12123/16587
https://sciforum.net/paper/view/16465
identifier_str_mv 2nd International Electronic Conference on Microbiology (Noviembre 2023)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Sciforum
publisher.none.fl_str_mv Sciforum
dc.source.none.fl_str_mv 2nd International Electronic Conference on Microbiology (Noviembre 2023)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.982451

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