Isolation and identification of culturable gut microbiota in the larval stage of lesser mealworm (Alphitobius diaperinus)
- Autores
- Antonuccio, Gisele Ivonne; Sauka, Diego Hernan
- Año de publicación
- 2023
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Poster y artículo
The highly prevalent pest Alphitobius diaperinus (Coleoptera: Tenebrionidae) causes significant structural damage in poultry farms. Despite previous investigations on its carriage of pathogenic microorganisms, our understanding of its microbiome remains limited. This study aimed to analyze the diversity of culturable gut microbiota in A. diaperinus obtained from laboratory breeding. Fifteen seventh instar larvae underwent a 24-hour starvation period, followed by surface disinfection. Dissected midguts were homogenized and plated on nutrient agar (NA), brain heart infusion agar (BHI), and Bacillus cereus agar (BC). The cultured isolates were subjected to gram staining, phylogenetic analysis, biochemical property evaluation, and metabolic activity assessment. Bacterial counts were higher in BHI (2.51x105 CFU/gut) than in NA (2.25x105 CFU/gut), possibly due to nutrient richness. NA exhibited a dominant colony morphology of gram-negative bacilli, while BHI displayed additional distinct colonies of gram-positive cocci. Surprisingly, yeast-like colonies were observed on BC plates. Based on 16S rRNA gene sequences, eight bacterial isolates were identified as Enterobacter sp., and two as Staphylococcus sp. Using RNA gene ITS region sequences, two yeast isolates were identified as Debaryomyces sp. and Hyphopichia sp. A preliminary species-level identification of bacteria (Enterobacter cloacae, Staphylococcus gallinarum, and Staphylococcus succinus) was achieved using API systems and complementary biochemical tests. Discrepancies between phylogenetic analysis and phenotypic data suggest the potential existence of new species or subspecies. Further comprehensive studies are required to confirm this hypothesis.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Antonuccio, Gisele Ivonne. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Antonuccio, Gisele Ivonne. Servicio Nacional de Sanidad y Calidad Agroalimentaria; Argentina
Fil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Sauka, Diego Herman. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Fuente
- 2nd International Electronic Conference on Microbiology (Noviembre 2023)
- Materia
-
Control Biológico
Biological Control
Yellow Mealworm
Microbial Flora
Gusano de la Harina
Alphitobius diaperinus
Flora Microbiana
Microbiota
Culturable Microorganisms
Bacterial Diversity - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/16587
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Isolation and identification of culturable gut microbiota in the larval stage of lesser mealworm (Alphitobius diaperinus)Antonuccio, Gisele IvonneSauka, Diego HernanControl BiológicoBiological ControlYellow MealwormMicrobial FloraGusano de la HarinaAlphitobius diaperinusFlora MicrobianaMicrobiotaCulturable MicroorganismsBacterial DiversityPoster y artículoThe highly prevalent pest Alphitobius diaperinus (Coleoptera: Tenebrionidae) causes significant structural damage in poultry farms. Despite previous investigations on its carriage of pathogenic microorganisms, our understanding of its microbiome remains limited. This study aimed to analyze the diversity of culturable gut microbiota in A. diaperinus obtained from laboratory breeding. Fifteen seventh instar larvae underwent a 24-hour starvation period, followed by surface disinfection. Dissected midguts were homogenized and plated on nutrient agar (NA), brain heart infusion agar (BHI), and Bacillus cereus agar (BC). The cultured isolates were subjected to gram staining, phylogenetic analysis, biochemical property evaluation, and metabolic activity assessment. Bacterial counts were higher in BHI (2.51x105 CFU/gut) than in NA (2.25x105 CFU/gut), possibly due to nutrient richness. NA exhibited a dominant colony morphology of gram-negative bacilli, while BHI displayed additional distinct colonies of gram-positive cocci. Surprisingly, yeast-like colonies were observed on BC plates. Based on 16S rRNA gene sequences, eight bacterial isolates were identified as Enterobacter sp., and two as Staphylococcus sp. Using RNA gene ITS region sequences, two yeast isolates were identified as Debaryomyces sp. and Hyphopichia sp. A preliminary species-level identification of bacteria (Enterobacter cloacae, Staphylococcus gallinarum, and Staphylococcus succinus) was achieved using API systems and complementary biochemical tests. Discrepancies between phylogenetic analysis and phenotypic data suggest the potential existence of new species or subspecies. Further comprehensive studies are required to confirm this hypothesis.Instituto de Microbiología y Zoología Agrícola (IMYZA)Fil: Antonuccio, Gisele Ivonne. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Antonuccio, Gisele Ivonne. Servicio Nacional de Sanidad y Calidad Agroalimentaria; ArgentinaFil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Sauka, Diego Herman. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaSciforum2024-02-14T10:46:22Z2024-02-14T10:46:22Z2023-11-30info:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfhttp://hdl.handle.net/20.500.12123/16587https://sciforum.net/paper/view/164652nd International Electronic Conference on Microbiology (Noviembre 2023)2nd International Electronic Conference on Microbiology (Noviembre 2023)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:46:19Zoai:localhost:20.500.12123/16587instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:46:19.673INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Isolation and identification of culturable gut microbiota in the larval stage of lesser mealworm (Alphitobius diaperinus) |
title |
Isolation and identification of culturable gut microbiota in the larval stage of lesser mealworm (Alphitobius diaperinus) |
spellingShingle |
Isolation and identification of culturable gut microbiota in the larval stage of lesser mealworm (Alphitobius diaperinus) Antonuccio, Gisele Ivonne Control Biológico Biological Control Yellow Mealworm Microbial Flora Gusano de la Harina Alphitobius diaperinus Flora Microbiana Microbiota Culturable Microorganisms Bacterial Diversity |
title_short |
Isolation and identification of culturable gut microbiota in the larval stage of lesser mealworm (Alphitobius diaperinus) |
title_full |
Isolation and identification of culturable gut microbiota in the larval stage of lesser mealworm (Alphitobius diaperinus) |
title_fullStr |
Isolation and identification of culturable gut microbiota in the larval stage of lesser mealworm (Alphitobius diaperinus) |
title_full_unstemmed |
Isolation and identification of culturable gut microbiota in the larval stage of lesser mealworm (Alphitobius diaperinus) |
title_sort |
Isolation and identification of culturable gut microbiota in the larval stage of lesser mealworm (Alphitobius diaperinus) |
dc.creator.none.fl_str_mv |
Antonuccio, Gisele Ivonne Sauka, Diego Hernan |
author |
Antonuccio, Gisele Ivonne |
author_facet |
Antonuccio, Gisele Ivonne Sauka, Diego Hernan |
author_role |
author |
author2 |
Sauka, Diego Hernan |
author2_role |
author |
dc.subject.none.fl_str_mv |
Control Biológico Biological Control Yellow Mealworm Microbial Flora Gusano de la Harina Alphitobius diaperinus Flora Microbiana Microbiota Culturable Microorganisms Bacterial Diversity |
topic |
Control Biológico Biological Control Yellow Mealworm Microbial Flora Gusano de la Harina Alphitobius diaperinus Flora Microbiana Microbiota Culturable Microorganisms Bacterial Diversity |
dc.description.none.fl_txt_mv |
Poster y artículo The highly prevalent pest Alphitobius diaperinus (Coleoptera: Tenebrionidae) causes significant structural damage in poultry farms. Despite previous investigations on its carriage of pathogenic microorganisms, our understanding of its microbiome remains limited. This study aimed to analyze the diversity of culturable gut microbiota in A. diaperinus obtained from laboratory breeding. Fifteen seventh instar larvae underwent a 24-hour starvation period, followed by surface disinfection. Dissected midguts were homogenized and plated on nutrient agar (NA), brain heart infusion agar (BHI), and Bacillus cereus agar (BC). The cultured isolates were subjected to gram staining, phylogenetic analysis, biochemical property evaluation, and metabolic activity assessment. Bacterial counts were higher in BHI (2.51x105 CFU/gut) than in NA (2.25x105 CFU/gut), possibly due to nutrient richness. NA exhibited a dominant colony morphology of gram-negative bacilli, while BHI displayed additional distinct colonies of gram-positive cocci. Surprisingly, yeast-like colonies were observed on BC plates. Based on 16S rRNA gene sequences, eight bacterial isolates were identified as Enterobacter sp., and two as Staphylococcus sp. Using RNA gene ITS region sequences, two yeast isolates were identified as Debaryomyces sp. and Hyphopichia sp. A preliminary species-level identification of bacteria (Enterobacter cloacae, Staphylococcus gallinarum, and Staphylococcus succinus) was achieved using API systems and complementary biochemical tests. Discrepancies between phylogenetic analysis and phenotypic data suggest the potential existence of new species or subspecies. Further comprehensive studies are required to confirm this hypothesis. Instituto de Microbiología y Zoología Agrícola (IMYZA) Fil: Antonuccio, Gisele Ivonne. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Antonuccio, Gisele Ivonne. Servicio Nacional de Sanidad y Calidad Agroalimentaria; Argentina Fil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Sauka, Diego Herman. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
description |
Poster y artículo |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-11-30 2024-02-14T10:46:22Z 2024-02-14T10:46:22Z |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/conferenceObject info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
format |
conferenceObject |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/16587 https://sciforum.net/paper/view/16465 2nd International Electronic Conference on Microbiology (Noviembre 2023) |
url |
http://hdl.handle.net/20.500.12123/16587 https://sciforum.net/paper/view/16465 |
identifier_str_mv |
2nd International Electronic Conference on Microbiology (Noviembre 2023) |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Sciforum |
publisher.none.fl_str_mv |
Sciforum |
dc.source.none.fl_str_mv |
2nd International Electronic Conference on Microbiology (Noviembre 2023) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
reponame_str |
INTA Digital (INTA) |
collection |
INTA Digital (INTA) |
instname_str |
Instituto Nacional de Tecnología Agropecuaria |
repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
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