Optimization of agitation and aeration conditions in bench-top scale stirred-tank bioreactor for maximum L-Dopa production

Autores
Peralta, Maria Patricia; Delgado, Osvaldo Daniel; Lechner, Bernardo Ernesto; Fariña, Julia Ines
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The main success of a bioreactor is simulating environmental conditions which are favorable for microbial growth, as well as for the secretion of enzymes and the production of further metabolites. Therefore, the bioprocess development requires to optimize the conditions under which this bioreactor is operated. As the volume scale is raised, aeration and agitation conditions become more compromised, so their optimization is a must. The objective of this work was to study the influence of aeration and agitation conditions during L-Dopa production at bench-top bioreactor scale by using the Paraboeremia LY 38.7 fungal isolate selected from Las Yungas Tucumanas. First, operative conditions of aeration (1 vvm), temperature (25 °C) and pH (pHi = 7) were kept constant, whilst varying agitation parameter between 250, 200 and 150 rpm. Once the agitation parameter was optimized, different aeration values were evaluated (0.5, 1.0 and 1.5 vvm). Production of L-Dopa and consumption of L-Tyrosine were measured by means the Arnow method, tyrosinase monophenolase and diphenolase activities according to the dopachrome method, and biomass was estimated as dry weight. From the set of tested conditions, an agitation speed of 200 rpm and an airflow rate of 1.0 vvm allowed obtaining the highest L-Dopa production (229,04 mg·L-1) in 56 h. At the higher aeration value (1.5 vvm) L-Dopa production became decreased (162,69 mg·L-1), and the same happened at the higher agitation set point (107,94 mg·L-1), along with the production of a polymer. At lower agitation and aeration, less production of L-Dopa (94,57 mg·L-1 and 130,94 mg·L-1) was found as well as less fungal growth, so that the decrease in production could be due to a lower biosynthetic machinery which depends on biomass. Selected conditions would be advantageous from the operational point of view, particularly considering the subsequent bioprocess scale-up, since they would imply lower operating costs.
Fil: Peralta, Maria Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Delgado, Osvaldo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Lechner, Bernardo Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
Fil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
LVI Reunión Anual Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular; XV Reunión Anual de Sociedad Argentina de Microbiología General
Ciudad Autónoma de Buenos Aries
Argentina
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Sociedad Argentina de Microbiología General
Materia
Agitación
Optimización
Bioreactores
Aireación
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/210605
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/210605
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Optimization of agitation and aeration conditions in bench-top scale stirred-tank bioreactor for maximum L-Dopa productionPeralta, Maria PatriciaDelgado, Osvaldo DanielLechner, Bernardo ErnestoFariña, Julia InesAgitaciónOptimizaciónBioreactoresAireaciónhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The main success of a bioreactor is simulating environmental conditions which are favorable for microbial growth, as well as for the secretion of enzymes and the production of further metabolites. Therefore, the bioprocess development requires to optimize the conditions under which this bioreactor is operated. As the volume scale is raised, aeration and agitation conditions become more compromised, so their optimization is a must. The objective of this work was to study the influence of aeration and agitation conditions during L-Dopa production at bench-top bioreactor scale by using the Paraboeremia LY 38.7 fungal isolate selected from Las Yungas Tucumanas. First, operative conditions of aeration (1 vvm), temperature (25 °C) and pH (pHi = 7) were kept constant, whilst varying agitation parameter between 250, 200 and 150 rpm. Once the agitation parameter was optimized, different aeration values were evaluated (0.5, 1.0 and 1.5 vvm). Production of L-Dopa and consumption of L-Tyrosine were measured by means the Arnow method, tyrosinase monophenolase and diphenolase activities according to the dopachrome method, and biomass was estimated as dry weight. From the set of tested conditions, an agitation speed of 200 rpm and an airflow rate of 1.0 vvm allowed obtaining the highest L-Dopa production (229,04 mg·L-1) in 56 h. At the higher aeration value (1.5 vvm) L-Dopa production became decreased (162,69 mg·L-1), and the same happened at the higher agitation set point (107,94 mg·L-1), along with the production of a polymer. At lower agitation and aeration, less production of L-Dopa (94,57 mg·L-1 and 130,94 mg·L-1) was found as well as less fungal growth, so that the decrease in production could be due to a lower biosynthetic machinery which depends on biomass. Selected conditions would be advantageous from the operational point of view, particularly considering the subsequent bioprocess scale-up, since they would imply lower operating costs.Fil: Peralta, Maria Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Delgado, Osvaldo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Lechner, Bernardo Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; ArgentinaFil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaLVI Reunión Anual Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular; XV Reunión Anual de Sociedad Argentina de Microbiología GeneralCiudad Autónoma de Buenos AriesArgentinaSociedad Argentina de Investigación Bioquímica y Biología MolecularSociedad Argentina de Microbiología GeneralTech Science Press2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/210605Optimization of agitation and aeration conditions in bench-top scale stirred-tank bioreactor for maximum L-Dopa production; LVI Reunión Anual Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular; XV Reunión Anual de Sociedad Argentina de Microbiología General; Ciudad Autónoma de Buenos Aries; Argentina; 2020; 153-1540327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/sites/default/files/BIOCELL-SAIB-2020-version-final.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:40:34Zoai:ri.conicet.gov.ar:11336/210605instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:40:34.544CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Optimization of agitation and aeration conditions in bench-top scale stirred-tank bioreactor for maximum L-Dopa production
title Optimization of agitation and aeration conditions in bench-top scale stirred-tank bioreactor for maximum L-Dopa production
spellingShingle Optimization of agitation and aeration conditions in bench-top scale stirred-tank bioreactor for maximum L-Dopa production
Peralta, Maria Patricia
Agitación
Optimización
Bioreactores
Aireación
title_short Optimization of agitation and aeration conditions in bench-top scale stirred-tank bioreactor for maximum L-Dopa production
title_full Optimization of agitation and aeration conditions in bench-top scale stirred-tank bioreactor for maximum L-Dopa production
title_fullStr Optimization of agitation and aeration conditions in bench-top scale stirred-tank bioreactor for maximum L-Dopa production
title_full_unstemmed Optimization of agitation and aeration conditions in bench-top scale stirred-tank bioreactor for maximum L-Dopa production
title_sort Optimization of agitation and aeration conditions in bench-top scale stirred-tank bioreactor for maximum L-Dopa production
dc.creator.none.fl_str_mv Peralta, Maria Patricia
Delgado, Osvaldo Daniel
Lechner, Bernardo Ernesto
Fariña, Julia Ines
author Peralta, Maria Patricia
author_facet Peralta, Maria Patricia
Delgado, Osvaldo Daniel
Lechner, Bernardo Ernesto
Fariña, Julia Ines
author_role author
author2 Delgado, Osvaldo Daniel
Lechner, Bernardo Ernesto
Fariña, Julia Ines
author2_role author
author
author
dc.subject.none.fl_str_mv Agitación
Optimización
Bioreactores
Aireación
topic Agitación
Optimización
Bioreactores
Aireación
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The main success of a bioreactor is simulating environmental conditions which are favorable for microbial growth, as well as for the secretion of enzymes and the production of further metabolites. Therefore, the bioprocess development requires to optimize the conditions under which this bioreactor is operated. As the volume scale is raised, aeration and agitation conditions become more compromised, so their optimization is a must. The objective of this work was to study the influence of aeration and agitation conditions during L-Dopa production at bench-top bioreactor scale by using the Paraboeremia LY 38.7 fungal isolate selected from Las Yungas Tucumanas. First, operative conditions of aeration (1 vvm), temperature (25 °C) and pH (pHi = 7) were kept constant, whilst varying agitation parameter between 250, 200 and 150 rpm. Once the agitation parameter was optimized, different aeration values were evaluated (0.5, 1.0 and 1.5 vvm). Production of L-Dopa and consumption of L-Tyrosine were measured by means the Arnow method, tyrosinase monophenolase and diphenolase activities according to the dopachrome method, and biomass was estimated as dry weight. From the set of tested conditions, an agitation speed of 200 rpm and an airflow rate of 1.0 vvm allowed obtaining the highest L-Dopa production (229,04 mg·L-1) in 56 h. At the higher aeration value (1.5 vvm) L-Dopa production became decreased (162,69 mg·L-1), and the same happened at the higher agitation set point (107,94 mg·L-1), along with the production of a polymer. At lower agitation and aeration, less production of L-Dopa (94,57 mg·L-1 and 130,94 mg·L-1) was found as well as less fungal growth, so that the decrease in production could be due to a lower biosynthetic machinery which depends on biomass. Selected conditions would be advantageous from the operational point of view, particularly considering the subsequent bioprocess scale-up, since they would imply lower operating costs.
Fil: Peralta, Maria Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Delgado, Osvaldo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Lechner, Bernardo Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
Fil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
LVI Reunión Anual Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular; XV Reunión Anual de Sociedad Argentina de Microbiología General
Ciudad Autónoma de Buenos Aries
Argentina
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Sociedad Argentina de Microbiología General
description The main success of a bioreactor is simulating environmental conditions which are favorable for microbial growth, as well as for the secretion of enzymes and the production of further metabolites. Therefore, the bioprocess development requires to optimize the conditions under which this bioreactor is operated. As the volume scale is raised, aeration and agitation conditions become more compromised, so their optimization is a must. The objective of this work was to study the influence of aeration and agitation conditions during L-Dopa production at bench-top bioreactor scale by using the Paraboeremia LY 38.7 fungal isolate selected from Las Yungas Tucumanas. First, operative conditions of aeration (1 vvm), temperature (25 °C) and pH (pHi = 7) were kept constant, whilst varying agitation parameter between 250, 200 and 150 rpm. Once the agitation parameter was optimized, different aeration values were evaluated (0.5, 1.0 and 1.5 vvm). Production of L-Dopa and consumption of L-Tyrosine were measured by means the Arnow method, tyrosinase monophenolase and diphenolase activities according to the dopachrome method, and biomass was estimated as dry weight. From the set of tested conditions, an agitation speed of 200 rpm and an airflow rate of 1.0 vvm allowed obtaining the highest L-Dopa production (229,04 mg·L-1) in 56 h. At the higher aeration value (1.5 vvm) L-Dopa production became decreased (162,69 mg·L-1), and the same happened at the higher agitation set point (107,94 mg·L-1), along with the production of a polymer. At lower agitation and aeration, less production of L-Dopa (94,57 mg·L-1 and 130,94 mg·L-1) was found as well as less fungal growth, so that the decrease in production could be due to a lower biosynthetic machinery which depends on biomass. Selected conditions would be advantageous from the operational point of view, particularly considering the subsequent bioprocess scale-up, since they would imply lower operating costs.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/210605
Optimization of agitation and aeration conditions in bench-top scale stirred-tank bioreactor for maximum L-Dopa production; LVI Reunión Anual Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular; XV Reunión Anual de Sociedad Argentina de Microbiología General; Ciudad Autónoma de Buenos Aries; Argentina; 2020; 153-154
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/210605
identifier_str_mv Optimization of agitation and aeration conditions in bench-top scale stirred-tank bioreactor for maximum L-Dopa production; LVI Reunión Anual Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular; XV Reunión Anual de Sociedad Argentina de Microbiología General; Ciudad Autónoma de Buenos Aries; Argentina; 2020; 153-154
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/sites/default/files/BIOCELL-SAIB-2020-version-final.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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