Biodegradation of microcystin-LR using acclimatized bacteria isolated from different units of the drinking water treatment plant

Autores
Kumar, Pratik; Hegde, Krishnamoorthy; Brar, Satinder Kaur; Cledón, Maximiliano; Kermanshahi-pour, Azadeh; Roy-Lachapelle, Audrey; Galvez-Cloutier, Rosa
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bacterial community isolated from different units of a Drinking Water Treatment Plant (DWTP) including pre-ozonation unit (POU), the effluent-sludge mixture of the sedimentation unit (ESSU) and top-sand layer water sample from the filtration unit (TSFU) were acclimatized separately in the microcystin-leucine arginine (MC-LR)-rich environment to evaluate MC-LR biodegradation. Maximum biodegradation efficiency of 97.2 ± 8.7% was achieved by the acclimatized-TSFU bacterial community followed by 72.1 ± 6.4% and 86.2 ± 7.3% by acclimatized-POU and acclimatized-ESSU bacterial community, respectively. Likewise, the non-acclimatized bacterial community showed similar biodegradation efficiency of 71.1 ± 7.37%, 86.7 ± 3.19% and 94.35 ± 10.63% for TSFU, ESSU and POU, respectively, when compared to the acclimatized ones. However, the biodegradation rate increased 1.5-folds for acclimatized versus non-acclimatized conditions. The mass spectrometry studies on MC-LR degradation depicted hydrolytic linearization of cyclic MC-LR along with the formation of small peptide fragments including Adda molecule that is linked to the reduced toxicity (qualitative toxicity analysis). This was further confirmed quantitatively by using Rhizobium meliloti as a bioindicator. The acclimatized-TSFU bacterial community comprised of novel MC-LR degrading strains, Chryseobacterium sp. and Pseudomonas fragi as confirmed by 16S rRNA sequencing. Biodegradation of microcystin-LR by in-situ bacterial community present in the drinking water treatment plant without formation of toxic by-product.
Fil: Kumar, Pratik. Université du Québec a Montreal; Canadá
Fil: Hegde, Krishnamoorthy. Université du Québec a Montreal; Canadá
Fil: Brar, Satinder Kaur. Université du Québec a Montreal; Canadá
Fil: Cledón, Maximiliano. Universidad Nacional del Comahue; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Kermanshahi-pour, Azadeh. Dalhousie University Halifax; Canadá
Fil: Roy-Lachapelle, Audrey. University of Montreal; Canadá
Fil: Galvez-Cloutier, Rosa. Laval University; Canadá
Materia
ACCLIMATIZED BACTERIA
DEGRADATION PATHWAY
DRINKING WATER
MICROCYSTIN
TOXICITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/99125
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/99125
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Biodegradation of microcystin-LR using acclimatized bacteria isolated from different units of the drinking water treatment plantKumar, PratikHegde, KrishnamoorthyBrar, Satinder KaurCledón, MaximilianoKermanshahi-pour, AzadehRoy-Lachapelle, AudreyGalvez-Cloutier, RosaACCLIMATIZED BACTERIADEGRADATION PATHWAYDRINKING WATERMICROCYSTINTOXICITYhttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2Bacterial community isolated from different units of a Drinking Water Treatment Plant (DWTP) including pre-ozonation unit (POU), the effluent-sludge mixture of the sedimentation unit (ESSU) and top-sand layer water sample from the filtration unit (TSFU) were acclimatized separately in the microcystin-leucine arginine (MC-LR)-rich environment to evaluate MC-LR biodegradation. Maximum biodegradation efficiency of 97.2 ± 8.7% was achieved by the acclimatized-TSFU bacterial community followed by 72.1 ± 6.4% and 86.2 ± 7.3% by acclimatized-POU and acclimatized-ESSU bacterial community, respectively. Likewise, the non-acclimatized bacterial community showed similar biodegradation efficiency of 71.1 ± 7.37%, 86.7 ± 3.19% and 94.35 ± 10.63% for TSFU, ESSU and POU, respectively, when compared to the acclimatized ones. However, the biodegradation rate increased 1.5-folds for acclimatized versus non-acclimatized conditions. The mass spectrometry studies on MC-LR degradation depicted hydrolytic linearization of cyclic MC-LR along with the formation of small peptide fragments including Adda molecule that is linked to the reduced toxicity (qualitative toxicity analysis). This was further confirmed quantitatively by using Rhizobium meliloti as a bioindicator. The acclimatized-TSFU bacterial community comprised of novel MC-LR degrading strains, Chryseobacterium sp. and Pseudomonas fragi as confirmed by 16S rRNA sequencing. Biodegradation of microcystin-LR by in-situ bacterial community present in the drinking water treatment plant without formation of toxic by-product.Fil: Kumar, Pratik. Université du Québec a Montreal; CanadáFil: Hegde, Krishnamoorthy. Université du Québec a Montreal; CanadáFil: Brar, Satinder Kaur. Université du Québec a Montreal; CanadáFil: Cledón, Maximiliano. Universidad Nacional del Comahue; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Kermanshahi-pour, Azadeh. Dalhousie University Halifax; CanadáFil: Roy-Lachapelle, Audrey. University of Montreal; CanadáFil: Galvez-Cloutier, Rosa. Laval University; CanadáElsevier2018-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/99125Kumar, Pratik; Hegde, Krishnamoorthy; Brar, Satinder Kaur; Cledón, Maximiliano; Kermanshahi-pour, Azadeh; et al.; Biodegradation of microcystin-LR using acclimatized bacteria isolated from different units of the drinking water treatment plant; Elsevier; Environmental Pollution; 242; Part A; 11-2018; 407-4160269-7491CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.envpol.2018.07.008info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0269749118314957info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:32:54Zoai:ri.conicet.gov.ar:11336/99125instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:32:55.197CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Biodegradation of microcystin-LR using acclimatized bacteria isolated from different units of the drinking water treatment plant
title Biodegradation of microcystin-LR using acclimatized bacteria isolated from different units of the drinking water treatment plant
spellingShingle Biodegradation of microcystin-LR using acclimatized bacteria isolated from different units of the drinking water treatment plant
Kumar, Pratik
ACCLIMATIZED BACTERIA
DEGRADATION PATHWAY
DRINKING WATER
MICROCYSTIN
TOXICITY
title_short Biodegradation of microcystin-LR using acclimatized bacteria isolated from different units of the drinking water treatment plant
title_full Biodegradation of microcystin-LR using acclimatized bacteria isolated from different units of the drinking water treatment plant
title_fullStr Biodegradation of microcystin-LR using acclimatized bacteria isolated from different units of the drinking water treatment plant
title_full_unstemmed Biodegradation of microcystin-LR using acclimatized bacteria isolated from different units of the drinking water treatment plant
title_sort Biodegradation of microcystin-LR using acclimatized bacteria isolated from different units of the drinking water treatment plant
dc.creator.none.fl_str_mv Kumar, Pratik
Hegde, Krishnamoorthy
Brar, Satinder Kaur
Cledón, Maximiliano
Kermanshahi-pour, Azadeh
Roy-Lachapelle, Audrey
Galvez-Cloutier, Rosa
author Kumar, Pratik
author_facet Kumar, Pratik
Hegde, Krishnamoorthy
Brar, Satinder Kaur
Cledón, Maximiliano
Kermanshahi-pour, Azadeh
Roy-Lachapelle, Audrey
Galvez-Cloutier, Rosa
author_role author
author2 Hegde, Krishnamoorthy
Brar, Satinder Kaur
Cledón, Maximiliano
Kermanshahi-pour, Azadeh
Roy-Lachapelle, Audrey
Galvez-Cloutier, Rosa
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ACCLIMATIZED BACTERIA
DEGRADATION PATHWAY
DRINKING WATER
MICROCYSTIN
TOXICITY
topic ACCLIMATIZED BACTERIA
DEGRADATION PATHWAY
DRINKING WATER
MICROCYSTIN
TOXICITY
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Bacterial community isolated from different units of a Drinking Water Treatment Plant (DWTP) including pre-ozonation unit (POU), the effluent-sludge mixture of the sedimentation unit (ESSU) and top-sand layer water sample from the filtration unit (TSFU) were acclimatized separately in the microcystin-leucine arginine (MC-LR)-rich environment to evaluate MC-LR biodegradation. Maximum biodegradation efficiency of 97.2 ± 8.7% was achieved by the acclimatized-TSFU bacterial community followed by 72.1 ± 6.4% and 86.2 ± 7.3% by acclimatized-POU and acclimatized-ESSU bacterial community, respectively. Likewise, the non-acclimatized bacterial community showed similar biodegradation efficiency of 71.1 ± 7.37%, 86.7 ± 3.19% and 94.35 ± 10.63% for TSFU, ESSU and POU, respectively, when compared to the acclimatized ones. However, the biodegradation rate increased 1.5-folds for acclimatized versus non-acclimatized conditions. The mass spectrometry studies on MC-LR degradation depicted hydrolytic linearization of cyclic MC-LR along with the formation of small peptide fragments including Adda molecule that is linked to the reduced toxicity (qualitative toxicity analysis). This was further confirmed quantitatively by using Rhizobium meliloti as a bioindicator. The acclimatized-TSFU bacterial community comprised of novel MC-LR degrading strains, Chryseobacterium sp. and Pseudomonas fragi as confirmed by 16S rRNA sequencing. Biodegradation of microcystin-LR by in-situ bacterial community present in the drinking water treatment plant without formation of toxic by-product.
Fil: Kumar, Pratik. Université du Québec a Montreal; Canadá
Fil: Hegde, Krishnamoorthy. Université du Québec a Montreal; Canadá
Fil: Brar, Satinder Kaur. Université du Québec a Montreal; Canadá
Fil: Cledón, Maximiliano. Universidad Nacional del Comahue; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Kermanshahi-pour, Azadeh. Dalhousie University Halifax; Canadá
Fil: Roy-Lachapelle, Audrey. University of Montreal; Canadá
Fil: Galvez-Cloutier, Rosa. Laval University; Canadá
description Bacterial community isolated from different units of a Drinking Water Treatment Plant (DWTP) including pre-ozonation unit (POU), the effluent-sludge mixture of the sedimentation unit (ESSU) and top-sand layer water sample from the filtration unit (TSFU) were acclimatized separately in the microcystin-leucine arginine (MC-LR)-rich environment to evaluate MC-LR biodegradation. Maximum biodegradation efficiency of 97.2 ± 8.7% was achieved by the acclimatized-TSFU bacterial community followed by 72.1 ± 6.4% and 86.2 ± 7.3% by acclimatized-POU and acclimatized-ESSU bacterial community, respectively. Likewise, the non-acclimatized bacterial community showed similar biodegradation efficiency of 71.1 ± 7.37%, 86.7 ± 3.19% and 94.35 ± 10.63% for TSFU, ESSU and POU, respectively, when compared to the acclimatized ones. However, the biodegradation rate increased 1.5-folds for acclimatized versus non-acclimatized conditions. The mass spectrometry studies on MC-LR degradation depicted hydrolytic linearization of cyclic MC-LR along with the formation of small peptide fragments including Adda molecule that is linked to the reduced toxicity (qualitative toxicity analysis). This was further confirmed quantitatively by using Rhizobium meliloti as a bioindicator. The acclimatized-TSFU bacterial community comprised of novel MC-LR degrading strains, Chryseobacterium sp. and Pseudomonas fragi as confirmed by 16S rRNA sequencing. Biodegradation of microcystin-LR by in-situ bacterial community present in the drinking water treatment plant without formation of toxic by-product.
publishDate 2018
dc.date.none.fl_str_mv 2018-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/99125
Kumar, Pratik; Hegde, Krishnamoorthy; Brar, Satinder Kaur; Cledón, Maximiliano; Kermanshahi-pour, Azadeh; et al.; Biodegradation of microcystin-LR using acclimatized bacteria isolated from different units of the drinking water treatment plant; Elsevier; Environmental Pollution; 242; Part A; 11-2018; 407-416
0269-7491
CONICET Digital
CONICET
url http://hdl.handle.net/11336/99125
identifier_str_mv Kumar, Pratik; Hegde, Krishnamoorthy; Brar, Satinder Kaur; Cledón, Maximiliano; Kermanshahi-pour, Azadeh; et al.; Biodegradation of microcystin-LR using acclimatized bacteria isolated from different units of the drinking water treatment plant; Elsevier; Environmental Pollution; 242; Part A; 11-2018; 407-416
0269-7491
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.envpol.2018.07.008
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0269749118314957
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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