Early responses to Fe-deficiency distinguish Sorghum bicolor genotypes with contrasting alkalinity tolerance

Autores
Luna, Dario Fernando; Saavedra Pons, Amalia Beatriz; Bustos, Dolores Angela; Taleisnik, Edith
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Soil alkalinity is a significant limitation to agricultural productivity and it is associated to several soil features, among them, Fe deficiencies. In this work, we explored the hypothesis that alkalinity tolerance in Sorghum bicolor is related to Fe-deficiency tolerance and its underlying mechanisms. An initial screening involving 8 sorghum genotypes identified two with contrasting growth responses to alkalinity (susceptible Minu II and more tolerant Silero INTA Pemán) that were subsequently studied under Fe-deprivation (-Fe) conditions. Sorghum sudanense (sudangrass) was included as control tolerant species for Fe deficiency. Growth in hydroponics and in soil indicated that responses to both alkaline and -Fe substrates followed parallel trends in the three genotypes: Minu II was the most sensitive, followed by Silero and sudangrass. Decreases in carbon fixation (A) and stomatal conductance were observed earlier in -Fe than in alkalinity, and the intensity in the three genotypes followed the same tendency as growth depressions. Calculations derived from the analysis of A as a function of internal CO2 concentration (A/Ci curves) indicated increased Ci concentration along with a decrease in the efficiency of phosphoenol pyruvate caboxylase activity in Minu II. Fast chlorophyll a fluorescence transients (OJIP-test) revealed decreased PSII connectivity in both Minu II and Silero under -Fe, but Minu II disclosed more damage to the oxygen evolving complex under alkalinity, while sudangrass was largely unresponsive. Expression of the genes for phytosiderophore (Phys) synthesis and transport genes was induced under both alkalinity and -Fe conditions in both S. bicolor genotypes, and more strongly in Silero than in Minu II. Lower induction of gene expression in Minu II may be related to its sensitivity to alkalinity conditions associated to reduced Fe availability, leading to alteration in photochemical and biochemical reactions involving Fe. Thus, our results provide support to the concept that susceptibility to Fe-deficiency and alkalinity conditions are associated in Sorghum bicolor and highlight some of the physiological traits that underlie this association.
Fil: Luna, Dario Fernando. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Saavedra Pons, Amalia Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Bustos, Dolores Angela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Taleisnik, Edith. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Materia
ALKALINITY TOLERANCE
CHLOROPHYLL FLUORESCENCE
FE-DEFICIENCY
OJIP PARAMETERS
PHYTOSIDEROPHORES
SORGHUM BICOLOR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/99083

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network_name_str CONICET Digital (CONICET)
spelling Early responses to Fe-deficiency distinguish Sorghum bicolor genotypes with contrasting alkalinity toleranceLuna, Dario FernandoSaavedra Pons, Amalia BeatrizBustos, Dolores AngelaTaleisnik, EdithALKALINITY TOLERANCECHLOROPHYLL FLUORESCENCEFE-DEFICIENCYOJIP PARAMETERSPHYTOSIDEROPHORESSORGHUM BICOLORhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Soil alkalinity is a significant limitation to agricultural productivity and it is associated to several soil features, among them, Fe deficiencies. In this work, we explored the hypothesis that alkalinity tolerance in Sorghum bicolor is related to Fe-deficiency tolerance and its underlying mechanisms. An initial screening involving 8 sorghum genotypes identified two with contrasting growth responses to alkalinity (susceptible Minu II and more tolerant Silero INTA Pemán) that were subsequently studied under Fe-deprivation (-Fe) conditions. Sorghum sudanense (sudangrass) was included as control tolerant species for Fe deficiency. Growth in hydroponics and in soil indicated that responses to both alkaline and -Fe substrates followed parallel trends in the three genotypes: Minu II was the most sensitive, followed by Silero and sudangrass. Decreases in carbon fixation (A) and stomatal conductance were observed earlier in -Fe than in alkalinity, and the intensity in the three genotypes followed the same tendency as growth depressions. Calculations derived from the analysis of A as a function of internal CO2 concentration (A/Ci curves) indicated increased Ci concentration along with a decrease in the efficiency of phosphoenol pyruvate caboxylase activity in Minu II. Fast chlorophyll a fluorescence transients (OJIP-test) revealed decreased PSII connectivity in both Minu II and Silero under -Fe, but Minu II disclosed more damage to the oxygen evolving complex under alkalinity, while sudangrass was largely unresponsive. Expression of the genes for phytosiderophore (Phys) synthesis and transport genes was induced under both alkalinity and -Fe conditions in both S. bicolor genotypes, and more strongly in Silero than in Minu II. Lower induction of gene expression in Minu II may be related to its sensitivity to alkalinity conditions associated to reduced Fe availability, leading to alteration in photochemical and biochemical reactions involving Fe. Thus, our results provide support to the concept that susceptibility to Fe-deficiency and alkalinity conditions are associated in Sorghum bicolor and highlight some of the physiological traits that underlie this association.Fil: Luna, Dario Fernando. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Saavedra Pons, Amalia Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Bustos, Dolores Angela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Taleisnik, Edith. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaPergamon-Elsevier Science Ltd2018-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/99083Luna, Dario Fernando; Saavedra Pons, Amalia Beatriz; Bustos, Dolores Angela; Taleisnik, Edith; Early responses to Fe-deficiency distinguish Sorghum bicolor genotypes with contrasting alkalinity tolerance; Pergamon-Elsevier Science Ltd; Environmental and Experimental Botany; 155; 11-2018; 165-1760098-8472CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0098847218304568info:eu-repo/semantics/altIdentifier/doi/10.1016/j.envexpbot.2018.06.030info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:38:34Zoai:ri.conicet.gov.ar:11336/99083instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:38:35.014CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Early responses to Fe-deficiency distinguish Sorghum bicolor genotypes with contrasting alkalinity tolerance
title Early responses to Fe-deficiency distinguish Sorghum bicolor genotypes with contrasting alkalinity tolerance
spellingShingle Early responses to Fe-deficiency distinguish Sorghum bicolor genotypes with contrasting alkalinity tolerance
Luna, Dario Fernando
ALKALINITY TOLERANCE
CHLOROPHYLL FLUORESCENCE
FE-DEFICIENCY
OJIP PARAMETERS
PHYTOSIDEROPHORES
SORGHUM BICOLOR
title_short Early responses to Fe-deficiency distinguish Sorghum bicolor genotypes with contrasting alkalinity tolerance
title_full Early responses to Fe-deficiency distinguish Sorghum bicolor genotypes with contrasting alkalinity tolerance
title_fullStr Early responses to Fe-deficiency distinguish Sorghum bicolor genotypes with contrasting alkalinity tolerance
title_full_unstemmed Early responses to Fe-deficiency distinguish Sorghum bicolor genotypes with contrasting alkalinity tolerance
title_sort Early responses to Fe-deficiency distinguish Sorghum bicolor genotypes with contrasting alkalinity tolerance
dc.creator.none.fl_str_mv Luna, Dario Fernando
Saavedra Pons, Amalia Beatriz
Bustos, Dolores Angela
Taleisnik, Edith
author Luna, Dario Fernando
author_facet Luna, Dario Fernando
Saavedra Pons, Amalia Beatriz
Bustos, Dolores Angela
Taleisnik, Edith
author_role author
author2 Saavedra Pons, Amalia Beatriz
Bustos, Dolores Angela
Taleisnik, Edith
author2_role author
author
author
dc.subject.none.fl_str_mv ALKALINITY TOLERANCE
CHLOROPHYLL FLUORESCENCE
FE-DEFICIENCY
OJIP PARAMETERS
PHYTOSIDEROPHORES
SORGHUM BICOLOR
topic ALKALINITY TOLERANCE
CHLOROPHYLL FLUORESCENCE
FE-DEFICIENCY
OJIP PARAMETERS
PHYTOSIDEROPHORES
SORGHUM BICOLOR
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Soil alkalinity is a significant limitation to agricultural productivity and it is associated to several soil features, among them, Fe deficiencies. In this work, we explored the hypothesis that alkalinity tolerance in Sorghum bicolor is related to Fe-deficiency tolerance and its underlying mechanisms. An initial screening involving 8 sorghum genotypes identified two with contrasting growth responses to alkalinity (susceptible Minu II and more tolerant Silero INTA Pemán) that were subsequently studied under Fe-deprivation (-Fe) conditions. Sorghum sudanense (sudangrass) was included as control tolerant species for Fe deficiency. Growth in hydroponics and in soil indicated that responses to both alkaline and -Fe substrates followed parallel trends in the three genotypes: Minu II was the most sensitive, followed by Silero and sudangrass. Decreases in carbon fixation (A) and stomatal conductance were observed earlier in -Fe than in alkalinity, and the intensity in the three genotypes followed the same tendency as growth depressions. Calculations derived from the analysis of A as a function of internal CO2 concentration (A/Ci curves) indicated increased Ci concentration along with a decrease in the efficiency of phosphoenol pyruvate caboxylase activity in Minu II. Fast chlorophyll a fluorescence transients (OJIP-test) revealed decreased PSII connectivity in both Minu II and Silero under -Fe, but Minu II disclosed more damage to the oxygen evolving complex under alkalinity, while sudangrass was largely unresponsive. Expression of the genes for phytosiderophore (Phys) synthesis and transport genes was induced under both alkalinity and -Fe conditions in both S. bicolor genotypes, and more strongly in Silero than in Minu II. Lower induction of gene expression in Minu II may be related to its sensitivity to alkalinity conditions associated to reduced Fe availability, leading to alteration in photochemical and biochemical reactions involving Fe. Thus, our results provide support to the concept that susceptibility to Fe-deficiency and alkalinity conditions are associated in Sorghum bicolor and highlight some of the physiological traits that underlie this association.
Fil: Luna, Dario Fernando. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Saavedra Pons, Amalia Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Bustos, Dolores Angela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Taleisnik, Edith. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
description Soil alkalinity is a significant limitation to agricultural productivity and it is associated to several soil features, among them, Fe deficiencies. In this work, we explored the hypothesis that alkalinity tolerance in Sorghum bicolor is related to Fe-deficiency tolerance and its underlying mechanisms. An initial screening involving 8 sorghum genotypes identified two with contrasting growth responses to alkalinity (susceptible Minu II and more tolerant Silero INTA Pemán) that were subsequently studied under Fe-deprivation (-Fe) conditions. Sorghum sudanense (sudangrass) was included as control tolerant species for Fe deficiency. Growth in hydroponics and in soil indicated that responses to both alkaline and -Fe substrates followed parallel trends in the three genotypes: Minu II was the most sensitive, followed by Silero and sudangrass. Decreases in carbon fixation (A) and stomatal conductance were observed earlier in -Fe than in alkalinity, and the intensity in the three genotypes followed the same tendency as growth depressions. Calculations derived from the analysis of A as a function of internal CO2 concentration (A/Ci curves) indicated increased Ci concentration along with a decrease in the efficiency of phosphoenol pyruvate caboxylase activity in Minu II. Fast chlorophyll a fluorescence transients (OJIP-test) revealed decreased PSII connectivity in both Minu II and Silero under -Fe, but Minu II disclosed more damage to the oxygen evolving complex under alkalinity, while sudangrass was largely unresponsive. Expression of the genes for phytosiderophore (Phys) synthesis and transport genes was induced under both alkalinity and -Fe conditions in both S. bicolor genotypes, and more strongly in Silero than in Minu II. Lower induction of gene expression in Minu II may be related to its sensitivity to alkalinity conditions associated to reduced Fe availability, leading to alteration in photochemical and biochemical reactions involving Fe. Thus, our results provide support to the concept that susceptibility to Fe-deficiency and alkalinity conditions are associated in Sorghum bicolor and highlight some of the physiological traits that underlie this association.
publishDate 2018
dc.date.none.fl_str_mv 2018-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/99083
Luna, Dario Fernando; Saavedra Pons, Amalia Beatriz; Bustos, Dolores Angela; Taleisnik, Edith; Early responses to Fe-deficiency distinguish Sorghum bicolor genotypes with contrasting alkalinity tolerance; Pergamon-Elsevier Science Ltd; Environmental and Experimental Botany; 155; 11-2018; 165-176
0098-8472
CONICET Digital
CONICET
url http://hdl.handle.net/11336/99083
identifier_str_mv Luna, Dario Fernando; Saavedra Pons, Amalia Beatriz; Bustos, Dolores Angela; Taleisnik, Edith; Early responses to Fe-deficiency distinguish Sorghum bicolor genotypes with contrasting alkalinity tolerance; Pergamon-Elsevier Science Ltd; Environmental and Experimental Botany; 155; 11-2018; 165-176
0098-8472
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0098847218304568
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.envexpbot.2018.06.030
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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