Early responses to Fe-deficiency distinguish Sorghum bicolor genotypes with contrasting alkalinity tolerance
- Autores
- Luna, Dario Fernando; Saavedra Pons, Amalia Beatriz; Bustos, Dolores Angela; Taleisnik, Edith
- Año de publicación
- 2018
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Soil alkalinity is a significant limitation to agricultural productivity and it is associated to several soil features, among them, Fe deficiencies. In this work, we explored the hypothesis that alkalinity tolerance in Sorghum bicolor is related to Fe-deficiency tolerance and its underlying mechanisms. An initial screening involving 8 sorghum genotypes identified two with contrasting growth responses to alkalinity (susceptible Minu II and more tolerant Silero INTA Pemán) that were subsequently studied under Fe-deprivation (-Fe) conditions. Sorghum sudanense (sudangrass) was included as control tolerant species for Fe deficiency. Growth in hydroponics and in soil indicated that responses to both alkaline and -Fe substrates followed parallel trends in the three genotypes: Minu II was the most sensitive, followed by Silero and sudangrass. Decreases in carbon fixation (A) and stomatal conductance were observed earlier in -Fe than in alkalinity, and the intensity in the three genotypes followed the same tendency as growth depressions. Calculations derived from the analysis of A as a function of internal CO2 concentration (A/Ci curves) indicated increased Ci concentration along with a decrease in the efficiency of phosphoenol pyruvate caboxylase activity in Minu II. Fast chlorophyll a fluorescence transients (OJIP-test) revealed decreased PSII connectivity in both Minu II and Silero under -Fe, but Minu II disclosed more damage to the oxygen evolving complex under alkalinity, while sudangrass was largely unresponsive. Expression of the genes for phytosiderophore (Phys) synthesis and transport genes was induced under both alkalinity and -Fe conditions in both S. bicolor genotypes, and more strongly in Silero than in Minu II. Lower induction of gene expression in Minu II may be related to its sensitivity to alkalinity conditions associated to reduced Fe availability, leading to alteration in photochemical and biochemical reactions involving Fe. Thus, our results provide support to the concept that susceptibility to Fe-deficiency and alkalinity conditions are associated in Sorghum bicolor and highlight some of the physiological traits that underlie this association.
Fil: Luna, Dario Fernando. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Saavedra Pons, Amalia Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Bustos, Dolores Angela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Taleisnik, Edith. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina - Materia
-
ALKALINITY TOLERANCE
CHLOROPHYLL FLUORESCENCE
FE-DEFICIENCY
OJIP PARAMETERS
PHYTOSIDEROPHORES
SORGHUM BICOLOR - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/99083
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Early responses to Fe-deficiency distinguish Sorghum bicolor genotypes with contrasting alkalinity toleranceLuna, Dario FernandoSaavedra Pons, Amalia BeatrizBustos, Dolores AngelaTaleisnik, EdithALKALINITY TOLERANCECHLOROPHYLL FLUORESCENCEFE-DEFICIENCYOJIP PARAMETERSPHYTOSIDEROPHORESSORGHUM BICOLORhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Soil alkalinity is a significant limitation to agricultural productivity and it is associated to several soil features, among them, Fe deficiencies. In this work, we explored the hypothesis that alkalinity tolerance in Sorghum bicolor is related to Fe-deficiency tolerance and its underlying mechanisms. An initial screening involving 8 sorghum genotypes identified two with contrasting growth responses to alkalinity (susceptible Minu II and more tolerant Silero INTA Pemán) that were subsequently studied under Fe-deprivation (-Fe) conditions. Sorghum sudanense (sudangrass) was included as control tolerant species for Fe deficiency. Growth in hydroponics and in soil indicated that responses to both alkaline and -Fe substrates followed parallel trends in the three genotypes: Minu II was the most sensitive, followed by Silero and sudangrass. Decreases in carbon fixation (A) and stomatal conductance were observed earlier in -Fe than in alkalinity, and the intensity in the three genotypes followed the same tendency as growth depressions. Calculations derived from the analysis of A as a function of internal CO2 concentration (A/Ci curves) indicated increased Ci concentration along with a decrease in the efficiency of phosphoenol pyruvate caboxylase activity in Minu II. Fast chlorophyll a fluorescence transients (OJIP-test) revealed decreased PSII connectivity in both Minu II and Silero under -Fe, but Minu II disclosed more damage to the oxygen evolving complex under alkalinity, while sudangrass was largely unresponsive. Expression of the genes for phytosiderophore (Phys) synthesis and transport genes was induced under both alkalinity and -Fe conditions in both S. bicolor genotypes, and more strongly in Silero than in Minu II. Lower induction of gene expression in Minu II may be related to its sensitivity to alkalinity conditions associated to reduced Fe availability, leading to alteration in photochemical and biochemical reactions involving Fe. Thus, our results provide support to the concept that susceptibility to Fe-deficiency and alkalinity conditions are associated in Sorghum bicolor and highlight some of the physiological traits that underlie this association.Fil: Luna, Dario Fernando. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Saavedra Pons, Amalia Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Bustos, Dolores Angela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Taleisnik, Edith. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaPergamon-Elsevier Science Ltd2018-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/99083Luna, Dario Fernando; Saavedra Pons, Amalia Beatriz; Bustos, Dolores Angela; Taleisnik, Edith; Early responses to Fe-deficiency distinguish Sorghum bicolor genotypes with contrasting alkalinity tolerance; Pergamon-Elsevier Science Ltd; Environmental and Experimental Botany; 155; 11-2018; 165-1760098-8472CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0098847218304568info:eu-repo/semantics/altIdentifier/doi/10.1016/j.envexpbot.2018.06.030info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:38:34Zoai:ri.conicet.gov.ar:11336/99083instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:38:35.014CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Early responses to Fe-deficiency distinguish Sorghum bicolor genotypes with contrasting alkalinity tolerance |
title |
Early responses to Fe-deficiency distinguish Sorghum bicolor genotypes with contrasting alkalinity tolerance |
spellingShingle |
Early responses to Fe-deficiency distinguish Sorghum bicolor genotypes with contrasting alkalinity tolerance Luna, Dario Fernando ALKALINITY TOLERANCE CHLOROPHYLL FLUORESCENCE FE-DEFICIENCY OJIP PARAMETERS PHYTOSIDEROPHORES SORGHUM BICOLOR |
title_short |
Early responses to Fe-deficiency distinguish Sorghum bicolor genotypes with contrasting alkalinity tolerance |
title_full |
Early responses to Fe-deficiency distinguish Sorghum bicolor genotypes with contrasting alkalinity tolerance |
title_fullStr |
Early responses to Fe-deficiency distinguish Sorghum bicolor genotypes with contrasting alkalinity tolerance |
title_full_unstemmed |
Early responses to Fe-deficiency distinguish Sorghum bicolor genotypes with contrasting alkalinity tolerance |
title_sort |
Early responses to Fe-deficiency distinguish Sorghum bicolor genotypes with contrasting alkalinity tolerance |
dc.creator.none.fl_str_mv |
Luna, Dario Fernando Saavedra Pons, Amalia Beatriz Bustos, Dolores Angela Taleisnik, Edith |
author |
Luna, Dario Fernando |
author_facet |
Luna, Dario Fernando Saavedra Pons, Amalia Beatriz Bustos, Dolores Angela Taleisnik, Edith |
author_role |
author |
author2 |
Saavedra Pons, Amalia Beatriz Bustos, Dolores Angela Taleisnik, Edith |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
ALKALINITY TOLERANCE CHLOROPHYLL FLUORESCENCE FE-DEFICIENCY OJIP PARAMETERS PHYTOSIDEROPHORES SORGHUM BICOLOR |
topic |
ALKALINITY TOLERANCE CHLOROPHYLL FLUORESCENCE FE-DEFICIENCY OJIP PARAMETERS PHYTOSIDEROPHORES SORGHUM BICOLOR |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Soil alkalinity is a significant limitation to agricultural productivity and it is associated to several soil features, among them, Fe deficiencies. In this work, we explored the hypothesis that alkalinity tolerance in Sorghum bicolor is related to Fe-deficiency tolerance and its underlying mechanisms. An initial screening involving 8 sorghum genotypes identified two with contrasting growth responses to alkalinity (susceptible Minu II and more tolerant Silero INTA Pemán) that were subsequently studied under Fe-deprivation (-Fe) conditions. Sorghum sudanense (sudangrass) was included as control tolerant species for Fe deficiency. Growth in hydroponics and in soil indicated that responses to both alkaline and -Fe substrates followed parallel trends in the three genotypes: Minu II was the most sensitive, followed by Silero and sudangrass. Decreases in carbon fixation (A) and stomatal conductance were observed earlier in -Fe than in alkalinity, and the intensity in the three genotypes followed the same tendency as growth depressions. Calculations derived from the analysis of A as a function of internal CO2 concentration (A/Ci curves) indicated increased Ci concentration along with a decrease in the efficiency of phosphoenol pyruvate caboxylase activity in Minu II. Fast chlorophyll a fluorescence transients (OJIP-test) revealed decreased PSII connectivity in both Minu II and Silero under -Fe, but Minu II disclosed more damage to the oxygen evolving complex under alkalinity, while sudangrass was largely unresponsive. Expression of the genes for phytosiderophore (Phys) synthesis and transport genes was induced under both alkalinity and -Fe conditions in both S. bicolor genotypes, and more strongly in Silero than in Minu II. Lower induction of gene expression in Minu II may be related to its sensitivity to alkalinity conditions associated to reduced Fe availability, leading to alteration in photochemical and biochemical reactions involving Fe. Thus, our results provide support to the concept that susceptibility to Fe-deficiency and alkalinity conditions are associated in Sorghum bicolor and highlight some of the physiological traits that underlie this association. Fil: Luna, Dario Fernando. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Saavedra Pons, Amalia Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina Fil: Bustos, Dolores Angela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Taleisnik, Edith. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina |
description |
Soil alkalinity is a significant limitation to agricultural productivity and it is associated to several soil features, among them, Fe deficiencies. In this work, we explored the hypothesis that alkalinity tolerance in Sorghum bicolor is related to Fe-deficiency tolerance and its underlying mechanisms. An initial screening involving 8 sorghum genotypes identified two with contrasting growth responses to alkalinity (susceptible Minu II and more tolerant Silero INTA Pemán) that were subsequently studied under Fe-deprivation (-Fe) conditions. Sorghum sudanense (sudangrass) was included as control tolerant species for Fe deficiency. Growth in hydroponics and in soil indicated that responses to both alkaline and -Fe substrates followed parallel trends in the three genotypes: Minu II was the most sensitive, followed by Silero and sudangrass. Decreases in carbon fixation (A) and stomatal conductance were observed earlier in -Fe than in alkalinity, and the intensity in the three genotypes followed the same tendency as growth depressions. Calculations derived from the analysis of A as a function of internal CO2 concentration (A/Ci curves) indicated increased Ci concentration along with a decrease in the efficiency of phosphoenol pyruvate caboxylase activity in Minu II. Fast chlorophyll a fluorescence transients (OJIP-test) revealed decreased PSII connectivity in both Minu II and Silero under -Fe, but Minu II disclosed more damage to the oxygen evolving complex under alkalinity, while sudangrass was largely unresponsive. Expression of the genes for phytosiderophore (Phys) synthesis and transport genes was induced under both alkalinity and -Fe conditions in both S. bicolor genotypes, and more strongly in Silero than in Minu II. Lower induction of gene expression in Minu II may be related to its sensitivity to alkalinity conditions associated to reduced Fe availability, leading to alteration in photochemical and biochemical reactions involving Fe. Thus, our results provide support to the concept that susceptibility to Fe-deficiency and alkalinity conditions are associated in Sorghum bicolor and highlight some of the physiological traits that underlie this association. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-11 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/99083 Luna, Dario Fernando; Saavedra Pons, Amalia Beatriz; Bustos, Dolores Angela; Taleisnik, Edith; Early responses to Fe-deficiency distinguish Sorghum bicolor genotypes with contrasting alkalinity tolerance; Pergamon-Elsevier Science Ltd; Environmental and Experimental Botany; 155; 11-2018; 165-176 0098-8472 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/99083 |
identifier_str_mv |
Luna, Dario Fernando; Saavedra Pons, Amalia Beatriz; Bustos, Dolores Angela; Taleisnik, Edith; Early responses to Fe-deficiency distinguish Sorghum bicolor genotypes with contrasting alkalinity tolerance; Pergamon-Elsevier Science Ltd; Environmental and Experimental Botany; 155; 11-2018; 165-176 0098-8472 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0098847218304568 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.envexpbot.2018.06.030 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Pergamon-Elsevier Science Ltd |
publisher.none.fl_str_mv |
Pergamon-Elsevier Science Ltd |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613220070850560 |
score |
13.070432 |