Early responses to Fe-deficiency distinguish Sorghum bicolor genotypes with contrasting alkalinity tolerance

Autores
Luna, Dario Fernando; Saavedra Pons, Amalia Beatriz; Bustos, Dolores Angela; Taleisnik, Edith
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Soil alkalinity is a significant limitation to agricultural productivity and it is associated to several soil features, among them, Fe deficiencies. In this work, we explored the hypothesis that alkalinity tolerance in Sorghum bicolor is related to Fe-deficiency tolerance and its underlying mechanisms. An initial screening involving 8 sorghum genotypes identified two with contrasting growth responses to alkalinity (susceptible Minu II and more tolerant Silero INTA Pemán) that were subsequently studied under Fe-deprivation (-Fe) conditions. Sorghum sudanense (sudangrass) was included as control tolerant species for Fe deficiency. Growth in hydroponics and in soil indicated that responses to both alkaline and -Fe substrates followed parallel trends in the three genotypes: Minu II was the most sensitive, followed by Silero and sudangrass. Decreases in carbon fixation (A) and stomatal conductance were observed earlier in -Fe than in alkalinity, and the intensity in the three genotypes followed the same tendency as growth depressions. Calculations derived from the analysis of A as a function of internal CO2 concentration (A/Ci curves) indicated increased Ci concentration along with a decrease in the efficiency of phosphoenol pyruvate caboxylase activity in Minu II. Fast chlorophyll a fluorescence transients (OJIP-test) revealed decreased PSII connectivity in both Minu II and Silero under -Fe, but Minu II disclosed more damage to the oxygen evolving complex under alkalinity, while sudangrass was largely unresponsive. Expression of the genes for phytosiderophore (Phys) synthesis and transport genes was induced under both alkalinity and -Fe conditions in both S. bicolor genotypes, and more strongly in Silero than in Minu II. Lower induction of gene expression in Minu II may be related to its sensitivity to alkalinity conditions associated to reduced Fe availability, leading to alteration in photochemical and biochemical reactions involving Fe. Thus, our results provide support to the concept that susceptibility to Fe-deficiency and alkalinity conditions are associated in Sorghum bicolor and highlight some of the physiological traits that underlie this association.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Luna, Dario Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Saavedra Pons, Amalia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Bustos, Dolores Angela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Taleisnik, Edith. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Consejo de Investigaciones Científicas y Técnicas; Argentina
Fuente
Environmental and experimental botany 155 : 165-176. (November 2018)
Materia
Sorghum Bicolor
Fitosideróforos
Alcalinidad
Tolerancia
Deficiencia de Minerales
Phytosiderophores
Alkalinity
Tolerance
Mineral Deficiencies
Chlorophyll Fluorescence
Fe Deficiency
OJIP
Fluorescencia Clorofílica
Deficiencia de Hierro
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3661

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oai_identifier_str oai:localhost:20.500.12123/3661
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spelling Early responses to Fe-deficiency distinguish Sorghum bicolor genotypes with contrasting alkalinity toleranceLuna, Dario FernandoSaavedra Pons, Amalia BeatrizBustos, Dolores AngelaTaleisnik, EdithSorghum BicolorFitosideróforosAlcalinidadToleranciaDeficiencia de MineralesPhytosiderophoresAlkalinityToleranceMineral DeficienciesChlorophyll FluorescenceFe DeficiencyOJIPFluorescencia ClorofílicaDeficiencia de HierroSoil alkalinity is a significant limitation to agricultural productivity and it is associated to several soil features, among them, Fe deficiencies. In this work, we explored the hypothesis that alkalinity tolerance in Sorghum bicolor is related to Fe-deficiency tolerance and its underlying mechanisms. An initial screening involving 8 sorghum genotypes identified two with contrasting growth responses to alkalinity (susceptible Minu II and more tolerant Silero INTA Pemán) that were subsequently studied under Fe-deprivation (-Fe) conditions. Sorghum sudanense (sudangrass) was included as control tolerant species for Fe deficiency. Growth in hydroponics and in soil indicated that responses to both alkaline and -Fe substrates followed parallel trends in the three genotypes: Minu II was the most sensitive, followed by Silero and sudangrass. Decreases in carbon fixation (A) and stomatal conductance were observed earlier in -Fe than in alkalinity, and the intensity in the three genotypes followed the same tendency as growth depressions. Calculations derived from the analysis of A as a function of internal CO2 concentration (A/Ci curves) indicated increased Ci concentration along with a decrease in the efficiency of phosphoenol pyruvate caboxylase activity in Minu II. Fast chlorophyll a fluorescence transients (OJIP-test) revealed decreased PSII connectivity in both Minu II and Silero under -Fe, but Minu II disclosed more damage to the oxygen evolving complex under alkalinity, while sudangrass was largely unresponsive. Expression of the genes for phytosiderophore (Phys) synthesis and transport genes was induced under both alkalinity and -Fe conditions in both S. bicolor genotypes, and more strongly in Silero than in Minu II. Lower induction of gene expression in Minu II may be related to its sensitivity to alkalinity conditions associated to reduced Fe availability, leading to alteration in photochemical and biochemical reactions involving Fe. Thus, our results provide support to the concept that susceptibility to Fe-deficiency and alkalinity conditions are associated in Sorghum bicolor and highlight some of the physiological traits that underlie this association.Instituto de Fisiología y Recursos Genéticos VegetalesFil: Luna, Dario Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Saavedra Pons, Amalia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Bustos, Dolores Angela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Taleisnik, Edith. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Consejo de Investigaciones Científicas y Técnicas; ArgentinaElsevier2018-10-22T15:05:35Z2018-10-22T15:05:35Z2018-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/3661https://www.sciencedirect.com/science/article/pii/S0098847218304568?via%3Dihub0098-8472https://doi.org/10.1016/j.envexpbot.2018.06.030Environmental and experimental botany 155 : 165-176. (November 2018)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:28Zoai:localhost:20.500.12123/3661instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:28.504INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Early responses to Fe-deficiency distinguish Sorghum bicolor genotypes with contrasting alkalinity tolerance
title Early responses to Fe-deficiency distinguish Sorghum bicolor genotypes with contrasting alkalinity tolerance
spellingShingle Early responses to Fe-deficiency distinguish Sorghum bicolor genotypes with contrasting alkalinity tolerance
Luna, Dario Fernando
Sorghum Bicolor
Fitosideróforos
Alcalinidad
Tolerancia
Deficiencia de Minerales
Phytosiderophores
Alkalinity
Tolerance
Mineral Deficiencies
Chlorophyll Fluorescence
Fe Deficiency
OJIP
Fluorescencia Clorofílica
Deficiencia de Hierro
title_short Early responses to Fe-deficiency distinguish Sorghum bicolor genotypes with contrasting alkalinity tolerance
title_full Early responses to Fe-deficiency distinguish Sorghum bicolor genotypes with contrasting alkalinity tolerance
title_fullStr Early responses to Fe-deficiency distinguish Sorghum bicolor genotypes with contrasting alkalinity tolerance
title_full_unstemmed Early responses to Fe-deficiency distinguish Sorghum bicolor genotypes with contrasting alkalinity tolerance
title_sort Early responses to Fe-deficiency distinguish Sorghum bicolor genotypes with contrasting alkalinity tolerance
dc.creator.none.fl_str_mv Luna, Dario Fernando
Saavedra Pons, Amalia Beatriz
Bustos, Dolores Angela
Taleisnik, Edith
author Luna, Dario Fernando
author_facet Luna, Dario Fernando
Saavedra Pons, Amalia Beatriz
Bustos, Dolores Angela
Taleisnik, Edith
author_role author
author2 Saavedra Pons, Amalia Beatriz
Bustos, Dolores Angela
Taleisnik, Edith
author2_role author
author
author
dc.subject.none.fl_str_mv Sorghum Bicolor
Fitosideróforos
Alcalinidad
Tolerancia
Deficiencia de Minerales
Phytosiderophores
Alkalinity
Tolerance
Mineral Deficiencies
Chlorophyll Fluorescence
Fe Deficiency
OJIP
Fluorescencia Clorofílica
Deficiencia de Hierro
topic Sorghum Bicolor
Fitosideróforos
Alcalinidad
Tolerancia
Deficiencia de Minerales
Phytosiderophores
Alkalinity
Tolerance
Mineral Deficiencies
Chlorophyll Fluorescence
Fe Deficiency
OJIP
Fluorescencia Clorofílica
Deficiencia de Hierro
dc.description.none.fl_txt_mv Soil alkalinity is a significant limitation to agricultural productivity and it is associated to several soil features, among them, Fe deficiencies. In this work, we explored the hypothesis that alkalinity tolerance in Sorghum bicolor is related to Fe-deficiency tolerance and its underlying mechanisms. An initial screening involving 8 sorghum genotypes identified two with contrasting growth responses to alkalinity (susceptible Minu II and more tolerant Silero INTA Pemán) that were subsequently studied under Fe-deprivation (-Fe) conditions. Sorghum sudanense (sudangrass) was included as control tolerant species for Fe deficiency. Growth in hydroponics and in soil indicated that responses to both alkaline and -Fe substrates followed parallel trends in the three genotypes: Minu II was the most sensitive, followed by Silero and sudangrass. Decreases in carbon fixation (A) and stomatal conductance were observed earlier in -Fe than in alkalinity, and the intensity in the three genotypes followed the same tendency as growth depressions. Calculations derived from the analysis of A as a function of internal CO2 concentration (A/Ci curves) indicated increased Ci concentration along with a decrease in the efficiency of phosphoenol pyruvate caboxylase activity in Minu II. Fast chlorophyll a fluorescence transients (OJIP-test) revealed decreased PSII connectivity in both Minu II and Silero under -Fe, but Minu II disclosed more damage to the oxygen evolving complex under alkalinity, while sudangrass was largely unresponsive. Expression of the genes for phytosiderophore (Phys) synthesis and transport genes was induced under both alkalinity and -Fe conditions in both S. bicolor genotypes, and more strongly in Silero than in Minu II. Lower induction of gene expression in Minu II may be related to its sensitivity to alkalinity conditions associated to reduced Fe availability, leading to alteration in photochemical and biochemical reactions involving Fe. Thus, our results provide support to the concept that susceptibility to Fe-deficiency and alkalinity conditions are associated in Sorghum bicolor and highlight some of the physiological traits that underlie this association.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Luna, Dario Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Saavedra Pons, Amalia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Bustos, Dolores Angela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Taleisnik, Edith. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Consejo de Investigaciones Científicas y Técnicas; Argentina
description Soil alkalinity is a significant limitation to agricultural productivity and it is associated to several soil features, among them, Fe deficiencies. In this work, we explored the hypothesis that alkalinity tolerance in Sorghum bicolor is related to Fe-deficiency tolerance and its underlying mechanisms. An initial screening involving 8 sorghum genotypes identified two with contrasting growth responses to alkalinity (susceptible Minu II and more tolerant Silero INTA Pemán) that were subsequently studied under Fe-deprivation (-Fe) conditions. Sorghum sudanense (sudangrass) was included as control tolerant species for Fe deficiency. Growth in hydroponics and in soil indicated that responses to both alkaline and -Fe substrates followed parallel trends in the three genotypes: Minu II was the most sensitive, followed by Silero and sudangrass. Decreases in carbon fixation (A) and stomatal conductance were observed earlier in -Fe than in alkalinity, and the intensity in the three genotypes followed the same tendency as growth depressions. Calculations derived from the analysis of A as a function of internal CO2 concentration (A/Ci curves) indicated increased Ci concentration along with a decrease in the efficiency of phosphoenol pyruvate caboxylase activity in Minu II. Fast chlorophyll a fluorescence transients (OJIP-test) revealed decreased PSII connectivity in both Minu II and Silero under -Fe, but Minu II disclosed more damage to the oxygen evolving complex under alkalinity, while sudangrass was largely unresponsive. Expression of the genes for phytosiderophore (Phys) synthesis and transport genes was induced under both alkalinity and -Fe conditions in both S. bicolor genotypes, and more strongly in Silero than in Minu II. Lower induction of gene expression in Minu II may be related to its sensitivity to alkalinity conditions associated to reduced Fe availability, leading to alteration in photochemical and biochemical reactions involving Fe. Thus, our results provide support to the concept that susceptibility to Fe-deficiency and alkalinity conditions are associated in Sorghum bicolor and highlight some of the physiological traits that underlie this association.
publishDate 2018
dc.date.none.fl_str_mv 2018-10-22T15:05:35Z
2018-10-22T15:05:35Z
2018-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/3661
https://www.sciencedirect.com/science/article/pii/S0098847218304568?via%3Dihub
0098-8472
https://doi.org/10.1016/j.envexpbot.2018.06.030
url http://hdl.handle.net/20.500.12123/3661
https://www.sciencedirect.com/science/article/pii/S0098847218304568?via%3Dihub
https://doi.org/10.1016/j.envexpbot.2018.06.030
identifier_str_mv 0098-8472
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Environmental and experimental botany 155 : 165-176. (November 2018)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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