Salicylic acid Is involved in the Nb-Mediated defense responses to potato virus X solanum tuberosum

Autores
Sánchez, Gerardo; Gerhardt, Nadia Cecilia; Siciliano, María Florencia; Vojnov, Adrián Alberto; Malcuit, Isabelle; Marano, María Rosa
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
To evaluate the role of salicylic acid (SA) in Nb -mediated hypersensitive resistance to Potato virus X (PVX) avirulent strain ROTH1 in Solanum tuberosum, we have constructed SA-deficient transgenic potato plant lines by overexpress-ing the bacterial enzyme salicylate hydroxylase (NahG), which degrades SA. Evaluation of these transgenic lines revealed hydrogen peroxide accumulation and spontaneous lesion formation in an age- and light-dependent manner. In concordance, NahG potato plants were more sensitive to treatment with methyl viologen, a reactive oxygen species-generating compound. In addition, when challenged with PVX ROTH1, NahG transgenic lines showed a decreased disease-resistance response to infection and were unable to induce systemic acquired resistance. However, the aviru-lent viral effector, the PVX 25-kDa protein, does induce expression of the pathogenesis-related gene PR-la in NahG potato plants. Taken together, our data indicate that SA is involved in local and systemic defense responses mediated by the Nb gene in Solanum tuberosum. This is the first report to show that basal levels of SA correlate with hyper-sensitive resistance to PVX.
Fil: Sánchez, Gerardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Gerhardt, Nadia Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Siciliano, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Vojnov, Adrián Alberto. Fundación Pablo Cassara; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Malcuit, Isabelle. Algentech; Reino Unido. Institute of Food Research; Reino Unido
Fil: Marano, María Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Materia
POTATO
NB
DEFENSE
RESISTANCE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/98440
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Salicylic acid Is involved in the Nb-Mediated defense responses to potato virus X solanum tuberosumSánchez, GerardoGerhardt, Nadia CeciliaSiciliano, María FlorenciaVojnov, Adrián AlbertoMalcuit, IsabelleMarano, María RosaPOTATONBDEFENSERESISTANCEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1To evaluate the role of salicylic acid (SA) in Nb -mediated hypersensitive resistance to Potato virus X (PVX) avirulent strain ROTH1 in Solanum tuberosum, we have constructed SA-deficient transgenic potato plant lines by overexpress-ing the bacterial enzyme salicylate hydroxylase (NahG), which degrades SA. Evaluation of these transgenic lines revealed hydrogen peroxide accumulation and spontaneous lesion formation in an age- and light-dependent manner. In concordance, NahG potato plants were more sensitive to treatment with methyl viologen, a reactive oxygen species-generating compound. In addition, when challenged with PVX ROTH1, NahG transgenic lines showed a decreased disease-resistance response to infection and were unable to induce systemic acquired resistance. However, the aviru-lent viral effector, the PVX 25-kDa protein, does induce expression of the pathogenesis-related gene PR-la in NahG potato plants. Taken together, our data indicate that SA is involved in local and systemic defense responses mediated by the Nb gene in Solanum tuberosum. This is the first report to show that basal levels of SA correlate with hyper-sensitive resistance to PVX.Fil: Sánchez, Gerardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Gerhardt, Nadia Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Siciliano, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Vojnov, Adrián Alberto. Fundación Pablo Cassara; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Malcuit, Isabelle. Algentech; Reino Unido. Institute of Food Research; Reino UnidoFil: Marano, María Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaAmerican Phytopathological Society2010-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/98440Sánchez, Gerardo; Gerhardt, Nadia Cecilia; Siciliano, María Florencia; Vojnov, Adrián Alberto; Malcuit, Isabelle; et al.; Salicylic acid Is involved in the Nb-Mediated defense responses to potato virus X solanum tuberosum; American Phytopathological Society; Molecular Plant-Microbe Interactions; 23; 4; 4-2010; 394-4050894-02821943-7706CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://apsjournals.apsnet.org/doi/abs/10.1094/MPMI-23-4-0394info:eu-repo/semantics/altIdentifier/doi/10.1094/MPMI-23-4-0394info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:07:38Zoai:ri.conicet.gov.ar:11336/98440instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:07:38.829CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Salicylic acid Is involved in the Nb-Mediated defense responses to potato virus X solanum tuberosum
title Salicylic acid Is involved in the Nb-Mediated defense responses to potato virus X solanum tuberosum
spellingShingle Salicylic acid Is involved in the Nb-Mediated defense responses to potato virus X solanum tuberosum
Sánchez, Gerardo
POTATO
NB
DEFENSE
RESISTANCE
title_short Salicylic acid Is involved in the Nb-Mediated defense responses to potato virus X solanum tuberosum
title_full Salicylic acid Is involved in the Nb-Mediated defense responses to potato virus X solanum tuberosum
title_fullStr Salicylic acid Is involved in the Nb-Mediated defense responses to potato virus X solanum tuberosum
title_full_unstemmed Salicylic acid Is involved in the Nb-Mediated defense responses to potato virus X solanum tuberosum
title_sort Salicylic acid Is involved in the Nb-Mediated defense responses to potato virus X solanum tuberosum
dc.creator.none.fl_str_mv Sánchez, Gerardo
Gerhardt, Nadia Cecilia
Siciliano, María Florencia
Vojnov, Adrián Alberto
Malcuit, Isabelle
Marano, María Rosa
author Sánchez, Gerardo
author_facet Sánchez, Gerardo
Gerhardt, Nadia Cecilia
Siciliano, María Florencia
Vojnov, Adrián Alberto
Malcuit, Isabelle
Marano, María Rosa
author_role author
author2 Gerhardt, Nadia Cecilia
Siciliano, María Florencia
Vojnov, Adrián Alberto
Malcuit, Isabelle
Marano, María Rosa
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv POTATO
NB
DEFENSE
RESISTANCE
topic POTATO
NB
DEFENSE
RESISTANCE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv To evaluate the role of salicylic acid (SA) in Nb -mediated hypersensitive resistance to Potato virus X (PVX) avirulent strain ROTH1 in Solanum tuberosum, we have constructed SA-deficient transgenic potato plant lines by overexpress-ing the bacterial enzyme salicylate hydroxylase (NahG), which degrades SA. Evaluation of these transgenic lines revealed hydrogen peroxide accumulation and spontaneous lesion formation in an age- and light-dependent manner. In concordance, NahG potato plants were more sensitive to treatment with methyl viologen, a reactive oxygen species-generating compound. In addition, when challenged with PVX ROTH1, NahG transgenic lines showed a decreased disease-resistance response to infection and were unable to induce systemic acquired resistance. However, the aviru-lent viral effector, the PVX 25-kDa protein, does induce expression of the pathogenesis-related gene PR-la in NahG potato plants. Taken together, our data indicate that SA is involved in local and systemic defense responses mediated by the Nb gene in Solanum tuberosum. This is the first report to show that basal levels of SA correlate with hyper-sensitive resistance to PVX.
Fil: Sánchez, Gerardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Gerhardt, Nadia Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Siciliano, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Vojnov, Adrián Alberto. Fundación Pablo Cassara; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Malcuit, Isabelle. Algentech; Reino Unido. Institute of Food Research; Reino Unido
Fil: Marano, María Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
description To evaluate the role of salicylic acid (SA) in Nb -mediated hypersensitive resistance to Potato virus X (PVX) avirulent strain ROTH1 in Solanum tuberosum, we have constructed SA-deficient transgenic potato plant lines by overexpress-ing the bacterial enzyme salicylate hydroxylase (NahG), which degrades SA. Evaluation of these transgenic lines revealed hydrogen peroxide accumulation and spontaneous lesion formation in an age- and light-dependent manner. In concordance, NahG potato plants were more sensitive to treatment with methyl viologen, a reactive oxygen species-generating compound. In addition, when challenged with PVX ROTH1, NahG transgenic lines showed a decreased disease-resistance response to infection and were unable to induce systemic acquired resistance. However, the aviru-lent viral effector, the PVX 25-kDa protein, does induce expression of the pathogenesis-related gene PR-la in NahG potato plants. Taken together, our data indicate that SA is involved in local and systemic defense responses mediated by the Nb gene in Solanum tuberosum. This is the first report to show that basal levels of SA correlate with hyper-sensitive resistance to PVX.
publishDate 2010
dc.date.none.fl_str_mv 2010-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/98440
Sánchez, Gerardo; Gerhardt, Nadia Cecilia; Siciliano, María Florencia; Vojnov, Adrián Alberto; Malcuit, Isabelle; et al.; Salicylic acid Is involved in the Nb-Mediated defense responses to potato virus X solanum tuberosum; American Phytopathological Society; Molecular Plant-Microbe Interactions; 23; 4; 4-2010; 394-405
0894-0282
1943-7706
CONICET Digital
CONICET
url http://hdl.handle.net/11336/98440
identifier_str_mv Sánchez, Gerardo; Gerhardt, Nadia Cecilia; Siciliano, María Florencia; Vojnov, Adrián Alberto; Malcuit, Isabelle; et al.; Salicylic acid Is involved in the Nb-Mediated defense responses to potato virus X solanum tuberosum; American Phytopathological Society; Molecular Plant-Microbe Interactions; 23; 4; 4-2010; 394-405
0894-0282
1943-7706
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://apsjournals.apsnet.org/doi/abs/10.1094/MPMI-23-4-0394
info:eu-repo/semantics/altIdentifier/doi/10.1094/MPMI-23-4-0394
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Phytopathological Society
publisher.none.fl_str_mv American Phytopathological Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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