Genomic instability in Solanum tuberosum x Solanum kurtzianum interspecific hybrids

Autores
Marfil, Carlos Federico; Masuelli, Ricardo Williams; Davison, Mario Ricardo; Comai, L.
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The use of interspecific crosses in breeding is an important strategy in improving the genetic base of the modern cultivated potato, Solanum tuberosum L. Until now, it has normally been interspecific Solanum hybrids that have been morphologically and cytologically characterized. However, little is known about the genomic changes that may occur in the hybrid nucleus owing to the combination of genomes of different origin. We have observed novel AFLP bands in Solanum tuberosum x Solanum kurtzianum diploid hybrids; 40 novel fragments were detected out of 138 AFLP fragments analyzed. No cytological abnormalities were observed in the hybrids; however, we found DNA methylation changes that could be the cause of the observed genomic instabilities. Of 277 MSAP fragments analyzed, 14% showed methylation patterns that differed between the parental species and the hybrids. We also observed frequent methylation changes in the BC1 progeny. Variation patterns among F1 and BC 1 plants suggest that some methylation changes occurred at random. The changes observed may have implications for potato breeding as an additional source of variability.
Le recours aux croisements interspécifiques constitue une importante stratégique en amélioration génétique pour accroître la diversité génétique chez la pomme de terre cultivée moderne, Solanum tuberosum L. Habituellement, les hybrides interspécifiques chez le genre Solanum ont été caractérisés sur les plans morphologique et cytologique. Cependant, nous en savons peu au sujet des changements génomiques qui pourraient se produire dans le noyau hybride en raison de la combinaison de génomes de diverses origines. Les auteurs ont observé de nouvelles bandes AFLP chez des hybrides diploïdes Solanum tuberosum × Solanum kurtzianum. Sur un total de 138 fragments AFLP, 40 produits nouveaux ont été détectés. Aucune anomalie cytologique a été observée chez les hybrides alors que des changements au niveau de la méthylation de l’ADN ont été observés et pourraient s’avérer la cause des instabilités génomiques observées. De 277 fragments MSAP, 14 % ont montré une méthylation différente entre les parents et les hybrides. Les auteurs ont aussi fréquemment observé des changements de méthylation au sein des progénitures BC1. La variation observée au sein des plantes F1 et BC1 suggère qu’une partie des changements de méthylation seraient survenus au hasard. Les changements observés pourraient avoir des implications pour la sélection en constituant une source additionnelle de variabilité.
Fil: Marfil, Carlos Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria La Consulta; Argentina
Fil: Masuelli, Ricardo Williams. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria La Consulta; Argentina
Fil: Davison, Mario Ricardo. University of Washington; Estados Unidos
Fil: Comai, L.. University of Washington; Estados Unidos
Materia
DNA METHYLATION
GENOME INSTABILITY
INTERSPECIFIC HYBRIDS
POTATO
SOLANUM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/124092
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/124092
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Genomic instability in Solanum tuberosum x Solanum kurtzianum interspecific hybridsMarfil, Carlos FedericoMasuelli, Ricardo WilliamsDavison, Mario RicardoComai, L.DNA METHYLATIONGENOME INSTABILITYINTERSPECIFIC HYBRIDSPOTATOSOLANUMhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The use of interspecific crosses in breeding is an important strategy in improving the genetic base of the modern cultivated potato, Solanum tuberosum L. Until now, it has normally been interspecific Solanum hybrids that have been morphologically and cytologically characterized. However, little is known about the genomic changes that may occur in the hybrid nucleus owing to the combination of genomes of different origin. We have observed novel AFLP bands in Solanum tuberosum x Solanum kurtzianum diploid hybrids; 40 novel fragments were detected out of 138 AFLP fragments analyzed. No cytological abnormalities were observed in the hybrids; however, we found DNA methylation changes that could be the cause of the observed genomic instabilities. Of 277 MSAP fragments analyzed, 14% showed methylation patterns that differed between the parental species and the hybrids. We also observed frequent methylation changes in the BC1 progeny. Variation patterns among F1 and BC 1 plants suggest that some methylation changes occurred at random. The changes observed may have implications for potato breeding as an additional source of variability.Le recours aux croisements interspécifiques constitue une importante stratégique en amélioration génétique pour accroître la diversité génétique chez la pomme de terre cultivée moderne, Solanum tuberosum L. Habituellement, les hybrides interspécifiques chez le genre Solanum ont été caractérisés sur les plans morphologique et cytologique. Cependant, nous en savons peu au sujet des changements génomiques qui pourraient se produire dans le noyau hybride en raison de la combinaison de génomes de diverses origines. Les auteurs ont observé de nouvelles bandes AFLP chez des hybrides diploïdes Solanum tuberosum × Solanum kurtzianum. Sur un total de 138 fragments AFLP, 40 produits nouveaux ont été détectés. Aucune anomalie cytologique a été observée chez les hybrides alors que des changements au niveau de la méthylation de l’ADN ont été observés et pourraient s’avérer la cause des instabilités génomiques observées. De 277 fragments MSAP, 14 % ont montré une méthylation différente entre les parents et les hybrides. Les auteurs ont aussi fréquemment observé des changements de méthylation au sein des progénitures BC1. La variation observée au sein des plantes F1 et BC1 suggère qu’une partie des changements de méthylation seraient survenus au hasard. Les changements observés pourraient avoir des implications pour la sélection en constituant une source additionnelle de variabilité.Fil: Marfil, Carlos Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria La Consulta; ArgentinaFil: Masuelli, Ricardo Williams. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria La Consulta; ArgentinaFil: Davison, Mario Ricardo. University of Washington; Estados UnidosFil: Comai, L.. University of Washington; Estados UnidosNational Research Council Canada-NRC Research Press2006-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/124092Marfil, Carlos Federico; Masuelli, Ricardo Williams; Davison, Mario Ricardo; Comai, L.; Genomic instability in Solanum tuberosum x Solanum kurtzianum interspecific hybrids; National Research Council Canada-NRC Research Press; Genome; 49; 2; 1-2-2006; 104-1130831-2796CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.nrcresearchpress.com/doi/10.1139/g05-088#.XtpPsjpKjIVinfo:eu-repo/semantics/altIdentifier/doi/10.1139/G05-088info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:02:16Zoai:ri.conicet.gov.ar:11336/124092instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:02:16.999CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genomic instability in Solanum tuberosum x Solanum kurtzianum interspecific hybrids
title Genomic instability in Solanum tuberosum x Solanum kurtzianum interspecific hybrids
spellingShingle Genomic instability in Solanum tuberosum x Solanum kurtzianum interspecific hybrids
Marfil, Carlos Federico
DNA METHYLATION
GENOME INSTABILITY
INTERSPECIFIC HYBRIDS
POTATO
SOLANUM
title_short Genomic instability in Solanum tuberosum x Solanum kurtzianum interspecific hybrids
title_full Genomic instability in Solanum tuberosum x Solanum kurtzianum interspecific hybrids
title_fullStr Genomic instability in Solanum tuberosum x Solanum kurtzianum interspecific hybrids
title_full_unstemmed Genomic instability in Solanum tuberosum x Solanum kurtzianum interspecific hybrids
title_sort Genomic instability in Solanum tuberosum x Solanum kurtzianum interspecific hybrids
dc.creator.none.fl_str_mv Marfil, Carlos Federico
Masuelli, Ricardo Williams
Davison, Mario Ricardo
Comai, L.
author Marfil, Carlos Federico
author_facet Marfil, Carlos Federico
Masuelli, Ricardo Williams
Davison, Mario Ricardo
Comai, L.
author_role author
author2 Masuelli, Ricardo Williams
Davison, Mario Ricardo
Comai, L.
author2_role author
author
author
dc.subject.none.fl_str_mv DNA METHYLATION
GENOME INSTABILITY
INTERSPECIFIC HYBRIDS
POTATO
SOLANUM
topic DNA METHYLATION
GENOME INSTABILITY
INTERSPECIFIC HYBRIDS
POTATO
SOLANUM
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The use of interspecific crosses in breeding is an important strategy in improving the genetic base of the modern cultivated potato, Solanum tuberosum L. Until now, it has normally been interspecific Solanum hybrids that have been morphologically and cytologically characterized. However, little is known about the genomic changes that may occur in the hybrid nucleus owing to the combination of genomes of different origin. We have observed novel AFLP bands in Solanum tuberosum x Solanum kurtzianum diploid hybrids; 40 novel fragments were detected out of 138 AFLP fragments analyzed. No cytological abnormalities were observed in the hybrids; however, we found DNA methylation changes that could be the cause of the observed genomic instabilities. Of 277 MSAP fragments analyzed, 14% showed methylation patterns that differed between the parental species and the hybrids. We also observed frequent methylation changes in the BC1 progeny. Variation patterns among F1 and BC 1 plants suggest that some methylation changes occurred at random. The changes observed may have implications for potato breeding as an additional source of variability.
Le recours aux croisements interspécifiques constitue une importante stratégique en amélioration génétique pour accroître la diversité génétique chez la pomme de terre cultivée moderne, Solanum tuberosum L. Habituellement, les hybrides interspécifiques chez le genre Solanum ont été caractérisés sur les plans morphologique et cytologique. Cependant, nous en savons peu au sujet des changements génomiques qui pourraient se produire dans le noyau hybride en raison de la combinaison de génomes de diverses origines. Les auteurs ont observé de nouvelles bandes AFLP chez des hybrides diploïdes Solanum tuberosum × Solanum kurtzianum. Sur un total de 138 fragments AFLP, 40 produits nouveaux ont été détectés. Aucune anomalie cytologique a été observée chez les hybrides alors que des changements au niveau de la méthylation de l’ADN ont été observés et pourraient s’avérer la cause des instabilités génomiques observées. De 277 fragments MSAP, 14 % ont montré une méthylation différente entre les parents et les hybrides. Les auteurs ont aussi fréquemment observé des changements de méthylation au sein des progénitures BC1. La variation observée au sein des plantes F1 et BC1 suggère qu’une partie des changements de méthylation seraient survenus au hasard. Les changements observés pourraient avoir des implications pour la sélection en constituant une source additionnelle de variabilité.
Fil: Marfil, Carlos Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria La Consulta; Argentina
Fil: Masuelli, Ricardo Williams. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria La Consulta; Argentina
Fil: Davison, Mario Ricardo. University of Washington; Estados Unidos
Fil: Comai, L.. University of Washington; Estados Unidos
description The use of interspecific crosses in breeding is an important strategy in improving the genetic base of the modern cultivated potato, Solanum tuberosum L. Until now, it has normally been interspecific Solanum hybrids that have been morphologically and cytologically characterized. However, little is known about the genomic changes that may occur in the hybrid nucleus owing to the combination of genomes of different origin. We have observed novel AFLP bands in Solanum tuberosum x Solanum kurtzianum diploid hybrids; 40 novel fragments were detected out of 138 AFLP fragments analyzed. No cytological abnormalities were observed in the hybrids; however, we found DNA methylation changes that could be the cause of the observed genomic instabilities. Of 277 MSAP fragments analyzed, 14% showed methylation patterns that differed between the parental species and the hybrids. We also observed frequent methylation changes in the BC1 progeny. Variation patterns among F1 and BC 1 plants suggest that some methylation changes occurred at random. The changes observed may have implications for potato breeding as an additional source of variability.
publishDate 2006
dc.date.none.fl_str_mv 2006-02-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/124092
Marfil, Carlos Federico; Masuelli, Ricardo Williams; Davison, Mario Ricardo; Comai, L.; Genomic instability in Solanum tuberosum x Solanum kurtzianum interspecific hybrids; National Research Council Canada-NRC Research Press; Genome; 49; 2; 1-2-2006; 104-113
0831-2796
CONICET Digital
CONICET
url http://hdl.handle.net/11336/124092
identifier_str_mv Marfil, Carlos Federico; Masuelli, Ricardo Williams; Davison, Mario Ricardo; Comai, L.; Genomic instability in Solanum tuberosum x Solanum kurtzianum interspecific hybrids; National Research Council Canada-NRC Research Press; Genome; 49; 2; 1-2-2006; 104-113
0831-2796
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.nrcresearchpress.com/doi/10.1139/g05-088#.XtpPsjpKjIV
info:eu-repo/semantics/altIdentifier/doi/10.1139/G05-088
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv National Research Council Canada-NRC Research Press
publisher.none.fl_str_mv National Research Council Canada-NRC Research Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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