Molecular techniques for Dicistrovirus detection without RNA extraction and purification

Autores
Querido, Jailson F. B.; Agirre, Jon; Marti, Gerardo Anibal; Guerin, Diego M. A.; Silva, Marcelo Sousa
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Dicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, we report a single-step method to identify TrV, a dicistrovirus, isolated from fecal samples of triatomines.The identification method proved to be quite sensitive, even without the extraction and purification of RNA virus.
Fil: Querido, Jailson F. B.. Universidade Nova de Lisboa; Portugal
Fil: Agirre, Jon. Universidad del Pais Vasco; España
Fil: Marti, Gerardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Estudios Parasitológicos y de Vectores (i); Argentina. Universidad Nacional de La Plata; Argentina
Fil: Guerin, Diego M. A.. Universidad del Pais Vasco; España
Fil: Silva, Marcelo Sousa . Universidade Nova de Lisboa; Portugal
Materia
Triatoma virus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/11284
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Molecular techniques for Dicistrovirus detection without RNA extraction and purificationQuerido, Jailson F. B.Agirre, JonMarti, Gerardo AnibalGuerin, Diego M. A.Silva, Marcelo Sousa Triatoma virushttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Dicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, we report a single-step method to identify TrV, a dicistrovirus, isolated from fecal samples of triatomines.The identification method proved to be quite sensitive, even without the extraction and purification of RNA virus.Fil: Querido, Jailson F. B.. Universidade Nova de Lisboa; PortugalFil: Agirre, Jon. Universidad del Pais Vasco; EspañaFil: Marti, Gerardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Estudios Parasitológicos y de Vectores (i); Argentina. Universidad Nacional de La Plata; ArgentinaFil: Guerin, Diego M. A.. Universidad del Pais Vasco; EspañaFil: Silva, Marcelo Sousa . Universidade Nova de Lisboa; PortugalHindawi Publishing Corporation2013-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/11284Querido, Jailson F. B.; Agirre, Jon; Marti, Gerardo Anibal; Guerin, Diego M. A.; Silva, Marcelo Sousa ; Molecular techniques for Dicistrovirus detection without RNA extraction and purification; Hindawi Publishing Corporation; Journal Of Biomedicine And Biotechnology; 2013; 3-2013; 1-31110-7243enginfo:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1155/2013/218593info:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/bmri/2013/218593/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:11:14Zoai:ri.conicet.gov.ar:11336/11284instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:11:15.093CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Molecular techniques for Dicistrovirus detection without RNA extraction and purification
title Molecular techniques for Dicistrovirus detection without RNA extraction and purification
spellingShingle Molecular techniques for Dicistrovirus detection without RNA extraction and purification
Querido, Jailson F. B.
Triatoma virus
title_short Molecular techniques for Dicistrovirus detection without RNA extraction and purification
title_full Molecular techniques for Dicistrovirus detection without RNA extraction and purification
title_fullStr Molecular techniques for Dicistrovirus detection without RNA extraction and purification
title_full_unstemmed Molecular techniques for Dicistrovirus detection without RNA extraction and purification
title_sort Molecular techniques for Dicistrovirus detection without RNA extraction and purification
dc.creator.none.fl_str_mv Querido, Jailson F. B.
Agirre, Jon
Marti, Gerardo Anibal
Guerin, Diego M. A.
Silva, Marcelo Sousa
author Querido, Jailson F. B.
author_facet Querido, Jailson F. B.
Agirre, Jon
Marti, Gerardo Anibal
Guerin, Diego M. A.
Silva, Marcelo Sousa
author_role author
author2 Agirre, Jon
Marti, Gerardo Anibal
Guerin, Diego M. A.
Silva, Marcelo Sousa
author2_role author
author
author
author
dc.subject.none.fl_str_mv Triatoma virus
topic Triatoma virus
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Dicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, we report a single-step method to identify TrV, a dicistrovirus, isolated from fecal samples of triatomines.The identification method proved to be quite sensitive, even without the extraction and purification of RNA virus.
Fil: Querido, Jailson F. B.. Universidade Nova de Lisboa; Portugal
Fil: Agirre, Jon. Universidad del Pais Vasco; España
Fil: Marti, Gerardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Estudios Parasitológicos y de Vectores (i); Argentina. Universidad Nacional de La Plata; Argentina
Fil: Guerin, Diego M. A.. Universidad del Pais Vasco; España
Fil: Silva, Marcelo Sousa . Universidade Nova de Lisboa; Portugal
description Dicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, we report a single-step method to identify TrV, a dicistrovirus, isolated from fecal samples of triatomines.The identification method proved to be quite sensitive, even without the extraction and purification of RNA virus.
publishDate 2013
dc.date.none.fl_str_mv 2013-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/11284
Querido, Jailson F. B.; Agirre, Jon; Marti, Gerardo Anibal; Guerin, Diego M. A.; Silva, Marcelo Sousa ; Molecular techniques for Dicistrovirus detection without RNA extraction and purification; Hindawi Publishing Corporation; Journal Of Biomedicine And Biotechnology; 2013; 3-2013; 1-3
1110-7243
url http://hdl.handle.net/11336/11284
identifier_str_mv Querido, Jailson F. B.; Agirre, Jon; Marti, Gerardo Anibal; Guerin, Diego M. A.; Silva, Marcelo Sousa ; Molecular techniques for Dicistrovirus detection without RNA extraction and purification; Hindawi Publishing Corporation; Journal Of Biomedicine And Biotechnology; 2013; 3-2013; 1-3
1110-7243
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1155/2013/218593
info:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/bmri/2013/218593/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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