Phylogenetics based on partial ORF2 of Triatoma Virus in Triatomines Collected Over a Decade from Domiciliary Habitats

Autores
Susevich, Maria Laura; Marti, Gerardo Anibal; Balsalobre, Agustin; Echeverria, Maria Gabriela
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The only virus sequenced and studied in triatomines is the Triatoma virus (TrV), from the the Dicistroviridae family which causes delayed development, reduced oviposition and premature death of infected insects. With the goal of expanding the sequences already obtained in previous years and verify if any changes occurred in their genomic sequences, 68 samples of triatomines from several provinces of Argentina were analyzed. Sixteen positive samples were obtained by RT-PCR using the VP3-VP1 subregion of ORF2 as a diagnostic method; after sequencing, 11 samples were obtained from T. infestans. These new sequences showed no significant differences in the analyzed regions, which were not grouped by species or habitat or geographical distribution. There were no differences when compared to the sequences found during 2002-2012, all obtained from the wild. We conclude that despite being an RNA virus, the different sequences show high homology.
Fil: Susevich, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Estudios Parasitológicos y de Vectores (i); Argentina
Fil: Marti, Gerardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Estudios Parasitológicos y de Vectores (i); Argentina
Fil: Balsalobre, Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Estudios Parasitológicos y de Vectores (i); Argentina
Fil: Echeverria, Maria Gabriela. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
triatoma infestans
triatoma virus
Argentina
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/11128
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Phylogenetics based on partial ORF2 of Triatoma Virus in Triatomines Collected Over a Decade from Domiciliary HabitatsSusevich, Maria LauraMarti, Gerardo AnibalBalsalobre, AgustinEcheverria, Maria Gabrielatriatoma infestanstriatoma virusArgentinahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The only virus sequenced and studied in triatomines is the Triatoma virus (TrV), from the the Dicistroviridae family which causes delayed development, reduced oviposition and premature death of infected insects. With the goal of expanding the sequences already obtained in previous years and verify if any changes occurred in their genomic sequences, 68 samples of triatomines from several provinces of Argentina were analyzed. Sixteen positive samples were obtained by RT-PCR using the VP3-VP1 subregion of ORF2 as a diagnostic method; after sequencing, 11 samples were obtained from T. infestans. These new sequences showed no significant differences in the analyzed regions, which were not grouped by species or habitat or geographical distribution. There were no differences when compared to the sequences found during 2002-2012, all obtained from the wild. We conclude that despite being an RNA virus, the different sequences show high homology.Fil: Susevich, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Estudios Parasitológicos y de Vectores (i); ArgentinaFil: Marti, Gerardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Estudios Parasitológicos y de Vectores (i); ArgentinaFil: Balsalobre, Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Estudios Parasitológicos y de Vectores (i); ArgentinaFil: Echeverria, Maria Gabriela. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaUniversity Of Arizona2015-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/11128Susevich, Maria Laura; Marti, Gerardo Anibal; Balsalobre, Agustin; Echeverria, Maria Gabriela; Phylogenetics based on partial ORF2 of Triatoma Virus in Triatomines Collected Over a Decade from Domiciliary Habitats; University Of Arizona; Journal Of Insect Science; 15; 1; 4-2015; 1-41536-2442enginfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4535133/info:eu-repo/semantics/altIdentifier/url/http://jinsectscience.oxfordjournals.org/content/15/1/17info:eu-repo/semantics/altIdentifier/doi/10.1093/jisesa/ieu139info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:01:51Zoai:ri.conicet.gov.ar:11336/11128instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:01:51.643CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Phylogenetics based on partial ORF2 of Triatoma Virus in Triatomines Collected Over a Decade from Domiciliary Habitats
title Phylogenetics based on partial ORF2 of Triatoma Virus in Triatomines Collected Over a Decade from Domiciliary Habitats
spellingShingle Phylogenetics based on partial ORF2 of Triatoma Virus in Triatomines Collected Over a Decade from Domiciliary Habitats
Susevich, Maria Laura
triatoma infestans
triatoma virus
Argentina
title_short Phylogenetics based on partial ORF2 of Triatoma Virus in Triatomines Collected Over a Decade from Domiciliary Habitats
title_full Phylogenetics based on partial ORF2 of Triatoma Virus in Triatomines Collected Over a Decade from Domiciliary Habitats
title_fullStr Phylogenetics based on partial ORF2 of Triatoma Virus in Triatomines Collected Over a Decade from Domiciliary Habitats
title_full_unstemmed Phylogenetics based on partial ORF2 of Triatoma Virus in Triatomines Collected Over a Decade from Domiciliary Habitats
title_sort Phylogenetics based on partial ORF2 of Triatoma Virus in Triatomines Collected Over a Decade from Domiciliary Habitats
dc.creator.none.fl_str_mv Susevich, Maria Laura
Marti, Gerardo Anibal
Balsalobre, Agustin
Echeverria, Maria Gabriela
author Susevich, Maria Laura
author_facet Susevich, Maria Laura
Marti, Gerardo Anibal
Balsalobre, Agustin
Echeverria, Maria Gabriela
author_role author
author2 Marti, Gerardo Anibal
Balsalobre, Agustin
Echeverria, Maria Gabriela
author2_role author
author
author
dc.subject.none.fl_str_mv triatoma infestans
triatoma virus
Argentina
topic triatoma infestans
triatoma virus
Argentina
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The only virus sequenced and studied in triatomines is the Triatoma virus (TrV), from the the Dicistroviridae family which causes delayed development, reduced oviposition and premature death of infected insects. With the goal of expanding the sequences already obtained in previous years and verify if any changes occurred in their genomic sequences, 68 samples of triatomines from several provinces of Argentina were analyzed. Sixteen positive samples were obtained by RT-PCR using the VP3-VP1 subregion of ORF2 as a diagnostic method; after sequencing, 11 samples were obtained from T. infestans. These new sequences showed no significant differences in the analyzed regions, which were not grouped by species or habitat or geographical distribution. There were no differences when compared to the sequences found during 2002-2012, all obtained from the wild. We conclude that despite being an RNA virus, the different sequences show high homology.
Fil: Susevich, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Estudios Parasitológicos y de Vectores (i); Argentina
Fil: Marti, Gerardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Estudios Parasitológicos y de Vectores (i); Argentina
Fil: Balsalobre, Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Estudios Parasitológicos y de Vectores (i); Argentina
Fil: Echeverria, Maria Gabriela. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The only virus sequenced and studied in triatomines is the Triatoma virus (TrV), from the the Dicistroviridae family which causes delayed development, reduced oviposition and premature death of infected insects. With the goal of expanding the sequences already obtained in previous years and verify if any changes occurred in their genomic sequences, 68 samples of triatomines from several provinces of Argentina were analyzed. Sixteen positive samples were obtained by RT-PCR using the VP3-VP1 subregion of ORF2 as a diagnostic method; after sequencing, 11 samples were obtained from T. infestans. These new sequences showed no significant differences in the analyzed regions, which were not grouped by species or habitat or geographical distribution. There were no differences when compared to the sequences found during 2002-2012, all obtained from the wild. We conclude that despite being an RNA virus, the different sequences show high homology.
publishDate 2015
dc.date.none.fl_str_mv 2015-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/11128
Susevich, Maria Laura; Marti, Gerardo Anibal; Balsalobre, Agustin; Echeverria, Maria Gabriela; Phylogenetics based on partial ORF2 of Triatoma Virus in Triatomines Collected Over a Decade from Domiciliary Habitats; University Of Arizona; Journal Of Insect Science; 15; 1; 4-2015; 1-4
1536-2442
url http://hdl.handle.net/11336/11128
identifier_str_mv Susevich, Maria Laura; Marti, Gerardo Anibal; Balsalobre, Agustin; Echeverria, Maria Gabriela; Phylogenetics based on partial ORF2 of Triatoma Virus in Triatomines Collected Over a Decade from Domiciliary Habitats; University Of Arizona; Journal Of Insect Science; 15; 1; 4-2015; 1-4
1536-2442
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4535133/
info:eu-repo/semantics/altIdentifier/url/http://jinsectscience.oxfordjournals.org/content/15/1/17
info:eu-repo/semantics/altIdentifier/doi/10.1093/jisesa/ieu139
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv University Of Arizona
publisher.none.fl_str_mv University Of Arizona
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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