Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification

Autores
Querido, Jailson F. B.; Agirre, Jon; Martí, Gerardo Aníbal; Guérin, Diego M. A.; Silva, Marcelo Sousa
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Dicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, we report a single-step method to identify TrV, a dicistrovirus, isolated from fecal samples of triatomines. The identification method proved to be quite sensitive, even without the extraction and purification of RNA virus.
Centro de Estudios Parasitológicos y de Vectores
Materia
Biología
Triatoma virus
Triatomines
Identification method
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/85477
Acceder
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network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Molecular Techniques for Dicistrovirus Detection without RNA Extraction or PurificationQuerido, Jailson F. B.Agirre, JonMartí, Gerardo AníbalGuérin, Diego M. A.Silva, Marcelo SousaBiologíaTriatoma virusTriatominesIdentification methodDicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, we report a single-step method to identify TrV, a dicistrovirus, isolated from fecal samples of triatomines. The identification method proved to be quite sensitive, even without the extraction and purification of RNA virus.Centro de Estudios Parasitológicos y de Vectores2013info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/85477enginfo:eu-repo/semantics/altIdentifier/issn/2314-6133info:eu-repo/semantics/altIdentifier/doi/10.1155/2013/218593info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-22T16:57:18Zoai:sedici.unlp.edu.ar:10915/85477Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-22 16:57:19.001SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification
title Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification
spellingShingle Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification
Querido, Jailson F. B.
Biología
Triatoma virus
Triatomines
Identification method
title_short Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification
title_full Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification
title_fullStr Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification
title_full_unstemmed Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification
title_sort Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification
dc.creator.none.fl_str_mv Querido, Jailson F. B.
Agirre, Jon
Martí, Gerardo Aníbal
Guérin, Diego M. A.
Silva, Marcelo Sousa
author Querido, Jailson F. B.
author_facet Querido, Jailson F. B.
Agirre, Jon
Martí, Gerardo Aníbal
Guérin, Diego M. A.
Silva, Marcelo Sousa
author_role author
author2 Agirre, Jon
Martí, Gerardo Aníbal
Guérin, Diego M. A.
Silva, Marcelo Sousa
author2_role author
author
author
author
dc.subject.none.fl_str_mv Biología
Triatoma virus
Triatomines
Identification method
topic Biología
Triatoma virus
Triatomines
Identification method
dc.description.none.fl_txt_mv Dicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, we report a single-step method to identify TrV, a dicistrovirus, isolated from fecal samples of triatomines. The identification method proved to be quite sensitive, even without the extraction and purification of RNA virus.
Centro de Estudios Parasitológicos y de Vectores
description Dicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, we report a single-step method to identify TrV, a dicistrovirus, isolated from fecal samples of triatomines. The identification method proved to be quite sensitive, even without the extraction and purification of RNA virus.
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/85477
url http://sedici.unlp.edu.ar/handle/10915/85477
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/2314-6133
info:eu-repo/semantics/altIdentifier/doi/10.1155/2013/218593
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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