Purification, crystallization and preliminary X-ray analysis of Triatoma virus (TrV) from Triatoma infestans

Autores
Rozas Dennis, Gabriela Susana; Squires, Gaëlle; Pous, Joan; Costabel, Marcelo Daniel; Lepault, Jean; Navaza, Jorge; Rey, Félix A.; Guérin, Diego Marcelo Alejandro
Año de publicación
2004
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Triatoma virus (TrV) is a viral pathogen of the blood-sucking reduviid bug Triatoma infestans, the most important vector of American human trypanosomiasis (Chagas' disease). TrV has been putatively classified as a member of the Cripavirus genus (type cricket paralysis virus) in the Dicistroviridae family. This work describes the purification of TrV particles from infected T. infestans and their crystallization and preliminary crystallographic analyses. Two different crystal forms, rhombohedral and orthorhombic, were obtained at room temperature by the hanging-drop vapour-diffusion technique using polyethylene glycol and polyethylene glycol monomethylether as precipitants. The rhombohedral crystals have unit-cell parameters a = b = 306.6, c = 788.4 Å (hexagonal setting), diffract to 3.2 Å resolution and contain one-third of the viral particle per asymmetric unit. The orthorhombic crystals have cell parameters a = 336, b = 351, c = 332 Å, diffract to about 2.5 Å resolution, and contain one-half of a virus particle in the asymmetric unit. A complete diffraction data set has been collected to 3.2 Å resolution, using synchrotron radiation, from a single rhombohedral crystal under cryogenic conditions. © 2004 International Union of Crystallography.
Fil: Rozas Dennis, Gabriela Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Física; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Squires, Gaëlle. Centre National de la Recherche Scientifique; Francia
Fil: Pous, Joan. Centre National de la Recherche Scientifique; Francia
Fil: Costabel, Marcelo Daniel. Universidad Nacional del Sur. Departamento de Física; Argentina
Fil: Lepault, Jean. Centre National de la Recherche Scientifique; Francia
Fil: Navaza, Jorge. Centre National de la Recherche Scientifique; Francia
Fil: Rey, Félix A.. Centre National de la Recherche Scientifique; Francia
Fil: Guérin, Diego Marcelo Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Física; Argentina
Materia
Triatoma Virus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/71607
Acceder
id CONICETDig_3832635186a373c52765150a72fbfc64
oai_identifier_str oai:ri.conicet.gov.ar:11336/71607
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Purification, crystallization and preliminary X-ray analysis of Triatoma virus (TrV) from Triatoma infestansRozas Dennis, Gabriela SusanaSquires, GaëllePous, JoanCostabel, Marcelo DanielLepault, JeanNavaza, JorgeRey, Félix A.Guérin, Diego Marcelo AlejandroTriatoma Virushttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Triatoma virus (TrV) is a viral pathogen of the blood-sucking reduviid bug Triatoma infestans, the most important vector of American human trypanosomiasis (Chagas' disease). TrV has been putatively classified as a member of the Cripavirus genus (type cricket paralysis virus) in the Dicistroviridae family. This work describes the purification of TrV particles from infected T. infestans and their crystallization and preliminary crystallographic analyses. Two different crystal forms, rhombohedral and orthorhombic, were obtained at room temperature by the hanging-drop vapour-diffusion technique using polyethylene glycol and polyethylene glycol monomethylether as precipitants. The rhombohedral crystals have unit-cell parameters a = b = 306.6, c = 788.4 Å (hexagonal setting), diffract to 3.2 Å resolution and contain one-third of the viral particle per asymmetric unit. The orthorhombic crystals have cell parameters a = 336, b = 351, c = 332 Å, diffract to about 2.5 Å resolution, and contain one-half of a virus particle in the asymmetric unit. A complete diffraction data set has been collected to 3.2 Å resolution, using synchrotron radiation, from a single rhombohedral crystal under cryogenic conditions. © 2004 International Union of Crystallography.Fil: Rozas Dennis, Gabriela Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Física; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Squires, Gaëlle. Centre National de la Recherche Scientifique; FranciaFil: Pous, Joan. Centre National de la Recherche Scientifique; FranciaFil: Costabel, Marcelo Daniel. Universidad Nacional del Sur. Departamento de Física; ArgentinaFil: Lepault, Jean. Centre National de la Recherche Scientifique; FranciaFil: Navaza, Jorge. Centre National de la Recherche Scientifique; FranciaFil: Rey, Félix A.. Centre National de la Recherche Scientifique; FranciaFil: Guérin, Diego Marcelo Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Física; ArgentinaWiley Blackwell Publishing, Inc2004-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/71607Rozas Dennis, Gabriela Susana; Squires, Gaëlle; Pous, Joan; Costabel, Marcelo Daniel; Lepault, Jean; et al.; Purification, crystallization and preliminary X-ray analysis of Triatoma virus (TrV) from Triatoma infestans; Wiley Blackwell Publishing, Inc; Acta Crystallographica Section D-Biological Crystallography; 60; 9; 9-2004; 1647-16500907-4449CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1107/S0907444904015185info:eu-repo/semantics/altIdentifier/url/http://scripts.iucr.org/cgi-bin/paper?S0907444904015185info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T10:42:53Zoai:ri.conicet.gov.ar:11336/71607instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 10:42:54.191CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Purification, crystallization and preliminary X-ray analysis of Triatoma virus (TrV) from Triatoma infestans
title Purification, crystallization and preliminary X-ray analysis of Triatoma virus (TrV) from Triatoma infestans
spellingShingle Purification, crystallization and preliminary X-ray analysis of Triatoma virus (TrV) from Triatoma infestans
Rozas Dennis, Gabriela Susana
Triatoma Virus
title_short Purification, crystallization and preliminary X-ray analysis of Triatoma virus (TrV) from Triatoma infestans
title_full Purification, crystallization and preliminary X-ray analysis of Triatoma virus (TrV) from Triatoma infestans
title_fullStr Purification, crystallization and preliminary X-ray analysis of Triatoma virus (TrV) from Triatoma infestans
title_full_unstemmed Purification, crystallization and preliminary X-ray analysis of Triatoma virus (TrV) from Triatoma infestans
title_sort Purification, crystallization and preliminary X-ray analysis of Triatoma virus (TrV) from Triatoma infestans
dc.creator.none.fl_str_mv Rozas Dennis, Gabriela Susana
Squires, Gaëlle
Pous, Joan
Costabel, Marcelo Daniel
Lepault, Jean
Navaza, Jorge
Rey, Félix A.
Guérin, Diego Marcelo Alejandro
author Rozas Dennis, Gabriela Susana
author_facet Rozas Dennis, Gabriela Susana
Squires, Gaëlle
Pous, Joan
Costabel, Marcelo Daniel
Lepault, Jean
Navaza, Jorge
Rey, Félix A.
Guérin, Diego Marcelo Alejandro
author_role author
author2 Squires, Gaëlle
Pous, Joan
Costabel, Marcelo Daniel
Lepault, Jean
Navaza, Jorge
Rey, Félix A.
Guérin, Diego Marcelo Alejandro
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Triatoma Virus
topic Triatoma Virus
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Triatoma virus (TrV) is a viral pathogen of the blood-sucking reduviid bug Triatoma infestans, the most important vector of American human trypanosomiasis (Chagas' disease). TrV has been putatively classified as a member of the Cripavirus genus (type cricket paralysis virus) in the Dicistroviridae family. This work describes the purification of TrV particles from infected T. infestans and their crystallization and preliminary crystallographic analyses. Two different crystal forms, rhombohedral and orthorhombic, were obtained at room temperature by the hanging-drop vapour-diffusion technique using polyethylene glycol and polyethylene glycol monomethylether as precipitants. The rhombohedral crystals have unit-cell parameters a = b = 306.6, c = 788.4 Å (hexagonal setting), diffract to 3.2 Å resolution and contain one-third of the viral particle per asymmetric unit. The orthorhombic crystals have cell parameters a = 336, b = 351, c = 332 Å, diffract to about 2.5 Å resolution, and contain one-half of a virus particle in the asymmetric unit. A complete diffraction data set has been collected to 3.2 Å resolution, using synchrotron radiation, from a single rhombohedral crystal under cryogenic conditions. © 2004 International Union of Crystallography.
Fil: Rozas Dennis, Gabriela Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Física; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Squires, Gaëlle. Centre National de la Recherche Scientifique; Francia
Fil: Pous, Joan. Centre National de la Recherche Scientifique; Francia
Fil: Costabel, Marcelo Daniel. Universidad Nacional del Sur. Departamento de Física; Argentina
Fil: Lepault, Jean. Centre National de la Recherche Scientifique; Francia
Fil: Navaza, Jorge. Centre National de la Recherche Scientifique; Francia
Fil: Rey, Félix A.. Centre National de la Recherche Scientifique; Francia
Fil: Guérin, Diego Marcelo Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Física; Argentina
description Triatoma virus (TrV) is a viral pathogen of the blood-sucking reduviid bug Triatoma infestans, the most important vector of American human trypanosomiasis (Chagas' disease). TrV has been putatively classified as a member of the Cripavirus genus (type cricket paralysis virus) in the Dicistroviridae family. This work describes the purification of TrV particles from infected T. infestans and their crystallization and preliminary crystallographic analyses. Two different crystal forms, rhombohedral and orthorhombic, were obtained at room temperature by the hanging-drop vapour-diffusion technique using polyethylene glycol and polyethylene glycol monomethylether as precipitants. The rhombohedral crystals have unit-cell parameters a = b = 306.6, c = 788.4 Å (hexagonal setting), diffract to 3.2 Å resolution and contain one-third of the viral particle per asymmetric unit. The orthorhombic crystals have cell parameters a = 336, b = 351, c = 332 Å, diffract to about 2.5 Å resolution, and contain one-half of a virus particle in the asymmetric unit. A complete diffraction data set has been collected to 3.2 Å resolution, using synchrotron radiation, from a single rhombohedral crystal under cryogenic conditions. © 2004 International Union of Crystallography.
publishDate 2004
dc.date.none.fl_str_mv 2004-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/71607
Rozas Dennis, Gabriela Susana; Squires, Gaëlle; Pous, Joan; Costabel, Marcelo Daniel; Lepault, Jean; et al.; Purification, crystallization and preliminary X-ray analysis of Triatoma virus (TrV) from Triatoma infestans; Wiley Blackwell Publishing, Inc; Acta Crystallographica Section D-Biological Crystallography; 60; 9; 9-2004; 1647-1650
0907-4449
CONICET Digital
CONICET
url http://hdl.handle.net/11336/71607
identifier_str_mv Rozas Dennis, Gabriela Susana; Squires, Gaëlle; Pous, Joan; Costabel, Marcelo Daniel; Lepault, Jean; et al.; Purification, crystallization and preliminary X-ray analysis of Triatoma virus (TrV) from Triatoma infestans; Wiley Blackwell Publishing, Inc; Acta Crystallographica Section D-Biological Crystallography; 60; 9; 9-2004; 1647-1650
0907-4449
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1107/S0907444904015185
info:eu-repo/semantics/altIdentifier/url/http://scripts.iucr.org/cgi-bin/paper?S0907444904015185
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1843605913263407104
score 13.001348

Publicaciones similares