Pseudomonas aeruginosa Dps (PA0962) Functions in H2O2 Mediated Oxidative Stress Defense and Exhibits In Vitro DNA Cleaving Activity

Autores
Rajapaksha, Nimesha; Soldano, Anabel; Yao, Huili; Donnarumma, Fabrizio; Kashipathy, Maithri M.; Seibold, Steve; Battaile, Kevin P.; Lovell, Scott; Rivera, Mario
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We report the structural, biochemical, and functional characterization of the product of gene PA0962 from Pseudomonas aeruginosa PAO1. The protein, termed Pa Dps, adopts the Dps subunit fold and oligomerizes into a nearly spherical 12-mer quaternary structure at pH 6.0 or in the presence of divalent cations at neutral pH and above. The 12-Mer Pa Dps contains two di-iron centers at the interface of each subunit dimer, coordinated by conserved His, Glu, and Asp residues. In vitro, the di-iron centers catalyze the oxidation of Fe2+ utilizing H2O2 (not O2) as an oxidant, suggesting Pa Dps functions to aid P. aeruginosa to survive H2O2-mediated oxidative stress. In agreement, a P. aeruginosa Δdps mutant is significantly more susceptible to H2O2 than the parent strain. The Pa Dps structure harbors a novel network of Tyr residues at the interface of each subunit dimer between the two di-iron centers, which captures radicals generated during Fe2+ oxidation at the ferroxidase centers and forms di-tyrosine linkages, thus effectively trapping the radicals within the Dps shell. Surprisingly, incubating Pa Dps and DNA revealed unprecedented DNA cleaving activity that is independent of H2O2 or O2 but requires divalent cations and 12-mer Pa Dps.
Fil: Rajapaksha, Nimesha. State University of Louisiana; Estados Unidos
Fil: Soldano, Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina. State University of Louisiana; Estados Unidos
Fil: Yao, Huili. State University of Louisiana; Estados Unidos
Fil: Donnarumma, Fabrizio. State University of Louisiana; Estados Unidos
Fil: Kashipathy, Maithri M.. University of Kansas; Estados Unidos
Fil: Seibold, Steve. University of Kansas; Estados Unidos
Fil: Battaile, Kevin P.. New York Structural Biology Center; Estados Unidos
Fil: Lovell, Scott. University of Kansas; Estados Unidos
Fil: Rivera, Mario. State University of Louisiana; Estados Unidos
Materia
DNA CLEAVING ACTIVITY
DPS
EXONUCLEASE
FERRITIN
IRON METABOLISM
MINI FERRITIN
OXIDATIVE STRESS
PEROXIDE TOXICITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/226732
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/226732
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Pseudomonas aeruginosa Dps (PA0962) Functions in H2O2 Mediated Oxidative Stress Defense and Exhibits In Vitro DNA Cleaving ActivityRajapaksha, NimeshaSoldano, AnabelYao, HuiliDonnarumma, FabrizioKashipathy, Maithri M.Seibold, SteveBattaile, Kevin P.Lovell, ScottRivera, MarioDNA CLEAVING ACTIVITYDPSEXONUCLEASEFERRITINIRON METABOLISMMINI FERRITINOXIDATIVE STRESSPEROXIDE TOXICITYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We report the structural, biochemical, and functional characterization of the product of gene PA0962 from Pseudomonas aeruginosa PAO1. The protein, termed Pa Dps, adopts the Dps subunit fold and oligomerizes into a nearly spherical 12-mer quaternary structure at pH 6.0 or in the presence of divalent cations at neutral pH and above. The 12-Mer Pa Dps contains two di-iron centers at the interface of each subunit dimer, coordinated by conserved His, Glu, and Asp residues. In vitro, the di-iron centers catalyze the oxidation of Fe2+ utilizing H2O2 (not O2) as an oxidant, suggesting Pa Dps functions to aid P. aeruginosa to survive H2O2-mediated oxidative stress. In agreement, a P. aeruginosa Δdps mutant is significantly more susceptible to H2O2 than the parent strain. The Pa Dps structure harbors a novel network of Tyr residues at the interface of each subunit dimer between the two di-iron centers, which captures radicals generated during Fe2+ oxidation at the ferroxidase centers and forms di-tyrosine linkages, thus effectively trapping the radicals within the Dps shell. Surprisingly, incubating Pa Dps and DNA revealed unprecedented DNA cleaving activity that is independent of H2O2 or O2 but requires divalent cations and 12-mer Pa Dps.Fil: Rajapaksha, Nimesha. State University of Louisiana; Estados UnidosFil: Soldano, Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina. State University of Louisiana; Estados UnidosFil: Yao, Huili. State University of Louisiana; Estados UnidosFil: Donnarumma, Fabrizio. State University of Louisiana; Estados UnidosFil: Kashipathy, Maithri M.. University of Kansas; Estados UnidosFil: Seibold, Steve. University of Kansas; Estados UnidosFil: Battaile, Kevin P.. New York Structural Biology Center; Estados UnidosFil: Lovell, Scott. University of Kansas; Estados UnidosFil: Rivera, Mario. State University of Louisiana; Estados UnidosMultidisciplinary Digital Publishing Institute2023-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/226732Rajapaksha, Nimesha; Soldano, Anabel; Yao, Huili; Donnarumma, Fabrizio; Kashipathy, Maithri M.; et al.; Pseudomonas aeruginosa Dps (PA0962) Functions in H2O2 Mediated Oxidative Stress Defense and Exhibits In Vitro DNA Cleaving Activity; Multidisciplinary Digital Publishing Institute; International Journal of Molecular Sciences; 24; 5; 3-2023; 1-201661-65961422-0067CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/24/5/4669info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms24054669info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:08:38Zoai:ri.conicet.gov.ar:11336/226732instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:08:38.54CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Pseudomonas aeruginosa Dps (PA0962) Functions in H2O2 Mediated Oxidative Stress Defense and Exhibits In Vitro DNA Cleaving Activity
title Pseudomonas aeruginosa Dps (PA0962) Functions in H2O2 Mediated Oxidative Stress Defense and Exhibits In Vitro DNA Cleaving Activity
spellingShingle Pseudomonas aeruginosa Dps (PA0962) Functions in H2O2 Mediated Oxidative Stress Defense and Exhibits In Vitro DNA Cleaving Activity
Rajapaksha, Nimesha
DNA CLEAVING ACTIVITY
DPS
EXONUCLEASE
FERRITIN
IRON METABOLISM
MINI FERRITIN
OXIDATIVE STRESS
PEROXIDE TOXICITY
title_short Pseudomonas aeruginosa Dps (PA0962) Functions in H2O2 Mediated Oxidative Stress Defense and Exhibits In Vitro DNA Cleaving Activity
title_full Pseudomonas aeruginosa Dps (PA0962) Functions in H2O2 Mediated Oxidative Stress Defense and Exhibits In Vitro DNA Cleaving Activity
title_fullStr Pseudomonas aeruginosa Dps (PA0962) Functions in H2O2 Mediated Oxidative Stress Defense and Exhibits In Vitro DNA Cleaving Activity
title_full_unstemmed Pseudomonas aeruginosa Dps (PA0962) Functions in H2O2 Mediated Oxidative Stress Defense and Exhibits In Vitro DNA Cleaving Activity
title_sort Pseudomonas aeruginosa Dps (PA0962) Functions in H2O2 Mediated Oxidative Stress Defense and Exhibits In Vitro DNA Cleaving Activity
dc.creator.none.fl_str_mv Rajapaksha, Nimesha
Soldano, Anabel
Yao, Huili
Donnarumma, Fabrizio
Kashipathy, Maithri M.
Seibold, Steve
Battaile, Kevin P.
Lovell, Scott
Rivera, Mario
author Rajapaksha, Nimesha
author_facet Rajapaksha, Nimesha
Soldano, Anabel
Yao, Huili
Donnarumma, Fabrizio
Kashipathy, Maithri M.
Seibold, Steve
Battaile, Kevin P.
Lovell, Scott
Rivera, Mario
author_role author
author2 Soldano, Anabel
Yao, Huili
Donnarumma, Fabrizio
Kashipathy, Maithri M.
Seibold, Steve
Battaile, Kevin P.
Lovell, Scott
Rivera, Mario
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv DNA CLEAVING ACTIVITY
DPS
EXONUCLEASE
FERRITIN
IRON METABOLISM
MINI FERRITIN
OXIDATIVE STRESS
PEROXIDE TOXICITY
topic DNA CLEAVING ACTIVITY
DPS
EXONUCLEASE
FERRITIN
IRON METABOLISM
MINI FERRITIN
OXIDATIVE STRESS
PEROXIDE TOXICITY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We report the structural, biochemical, and functional characterization of the product of gene PA0962 from Pseudomonas aeruginosa PAO1. The protein, termed Pa Dps, adopts the Dps subunit fold and oligomerizes into a nearly spherical 12-mer quaternary structure at pH 6.0 or in the presence of divalent cations at neutral pH and above. The 12-Mer Pa Dps contains two di-iron centers at the interface of each subunit dimer, coordinated by conserved His, Glu, and Asp residues. In vitro, the di-iron centers catalyze the oxidation of Fe2+ utilizing H2O2 (not O2) as an oxidant, suggesting Pa Dps functions to aid P. aeruginosa to survive H2O2-mediated oxidative stress. In agreement, a P. aeruginosa Δdps mutant is significantly more susceptible to H2O2 than the parent strain. The Pa Dps structure harbors a novel network of Tyr residues at the interface of each subunit dimer between the two di-iron centers, which captures radicals generated during Fe2+ oxidation at the ferroxidase centers and forms di-tyrosine linkages, thus effectively trapping the radicals within the Dps shell. Surprisingly, incubating Pa Dps and DNA revealed unprecedented DNA cleaving activity that is independent of H2O2 or O2 but requires divalent cations and 12-mer Pa Dps.
Fil: Rajapaksha, Nimesha. State University of Louisiana; Estados Unidos
Fil: Soldano, Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina. State University of Louisiana; Estados Unidos
Fil: Yao, Huili. State University of Louisiana; Estados Unidos
Fil: Donnarumma, Fabrizio. State University of Louisiana; Estados Unidos
Fil: Kashipathy, Maithri M.. University of Kansas; Estados Unidos
Fil: Seibold, Steve. University of Kansas; Estados Unidos
Fil: Battaile, Kevin P.. New York Structural Biology Center; Estados Unidos
Fil: Lovell, Scott. University of Kansas; Estados Unidos
Fil: Rivera, Mario. State University of Louisiana; Estados Unidos
description We report the structural, biochemical, and functional characterization of the product of gene PA0962 from Pseudomonas aeruginosa PAO1. The protein, termed Pa Dps, adopts the Dps subunit fold and oligomerizes into a nearly spherical 12-mer quaternary structure at pH 6.0 or in the presence of divalent cations at neutral pH and above. The 12-Mer Pa Dps contains two di-iron centers at the interface of each subunit dimer, coordinated by conserved His, Glu, and Asp residues. In vitro, the di-iron centers catalyze the oxidation of Fe2+ utilizing H2O2 (not O2) as an oxidant, suggesting Pa Dps functions to aid P. aeruginosa to survive H2O2-mediated oxidative stress. In agreement, a P. aeruginosa Δdps mutant is significantly more susceptible to H2O2 than the parent strain. The Pa Dps structure harbors a novel network of Tyr residues at the interface of each subunit dimer between the two di-iron centers, which captures radicals generated during Fe2+ oxidation at the ferroxidase centers and forms di-tyrosine linkages, thus effectively trapping the radicals within the Dps shell. Surprisingly, incubating Pa Dps and DNA revealed unprecedented DNA cleaving activity that is independent of H2O2 or O2 but requires divalent cations and 12-mer Pa Dps.
publishDate 2023
dc.date.none.fl_str_mv 2023-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/226732
Rajapaksha, Nimesha; Soldano, Anabel; Yao, Huili; Donnarumma, Fabrizio; Kashipathy, Maithri M.; et al.; Pseudomonas aeruginosa Dps (PA0962) Functions in H2O2 Mediated Oxidative Stress Defense and Exhibits In Vitro DNA Cleaving Activity; Multidisciplinary Digital Publishing Institute; International Journal of Molecular Sciences; 24; 5; 3-2023; 1-20
1661-6596
1422-0067
CONICET Digital
CONICET
url http://hdl.handle.net/11336/226732
identifier_str_mv Rajapaksha, Nimesha; Soldano, Anabel; Yao, Huili; Donnarumma, Fabrizio; Kashipathy, Maithri M.; et al.; Pseudomonas aeruginosa Dps (PA0962) Functions in H2O2 Mediated Oxidative Stress Defense and Exhibits In Vitro DNA Cleaving Activity; Multidisciplinary Digital Publishing Institute; International Journal of Molecular Sciences; 24; 5; 3-2023; 1-20
1661-6596
1422-0067
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/24/5/4669
info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms24054669
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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