Diclofenac and caffeine inhibit hepatic antioxidant enzymes in the freshwater fish Astyanax altiparanae (Teleostei: Characiformes)

Autores
Muñoz Peñuela, Marcela; Lo Nostro, Fabiana Laura; Dal'Olio Gomes, Aline; Tolussi, Carlos Eduardo; Branco, Giovana Souza; Pinheiro, João Paulo Silva; Godoi, Filipe Guilherme Andrade de; Moreira, Renata Guimarães
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Although concentrations of pharmaceutical compounds in aquatic ecosystems are low, they can cause toxic effects on organisms. The aim of this study was to evaluate the effects of diclofenac (DCF), a non-steroidal anti-inflammatory drug, and caffeine (CAF), a central nervous system stimulant, both alone or combined, in Astyanax altiparanae males under acute exposure (96 h), measuring neurotoxicity biomarkers, antioxidant response and damage at biochemical and cellular levels. DCF concentration in water, separated and combined, was 3.08 mg L−1 and that of CAF was 9.59 mg L−1. To assess neurotoxicity, brain and muscle acetylcholinesterase (AChE) activities were measured. To evaluate oxidative stress, the enzymatic activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and glutathione S-transferase (GST), as well as lipoperoxidation (LPO), were analyzed in liver and gills. Activity of hepatic cyclooxygenase (COX) was also evaluated. Genotoxicity was assessed in blood using comet assay and micronucleus test, as well as nuclear abnormalities. DCF and CAF, alone or combined, had neither effect on AChE activity, nor in the activity of SOD, CAT, GPx and GST in gills. In liver, DCF inhibited SOD and GPx activity, CAF inhibited CAT activity, the mixture inhibited SOD and GST activity; although only fish exposed to CAF showed increased hepatic LPO. Under these experimental conditions, no effect on COX activity was observed, nor cytotoxic and genotoxic damage. The most pronounced effects were caused by the drugs separately, since both compounds altered the enzymes, but only CAF triggered LPO, showing more harmful effects.
Fil: Muñoz Peñuela, Marcela. Universidade de Sao Paulo; Brasil
Fil: Lo Nostro, Fabiana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina
Fil: Dal'Olio Gomes, Aline. Universidade de Sao Paulo; Brasil
Fil: Tolussi, Carlos Eduardo. Universidade Anhembi Morumbi; Brasil
Fil: Branco, Giovana Souza. Universidade de Sao Paulo; Brasil
Fil: Pinheiro, João Paulo Silva. Universidade de Sao Paulo; Brasil
Fil: Godoi, Filipe Guilherme Andrade de. Universidade de Sao Paulo; Brasil
Fil: Moreira, Renata Guimarães. Universidade de Sao Paulo; Brasil
Materia
DRUGS
GENOTOXICITY
LIPOPEROXIDATION
OXIDATIVE STRESS
WATER POLLUTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/181633
Acceder
id CONICETDig_3dfe1b425fd53dc44028fe69c2da4a1e
oai_identifier_str oai:ri.conicet.gov.ar:11336/181633
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Diclofenac and caffeine inhibit hepatic antioxidant enzymes in the freshwater fish Astyanax altiparanae (Teleostei: Characiformes)Muñoz Peñuela, MarcelaLo Nostro, Fabiana LauraDal'Olio Gomes, AlineTolussi, Carlos EduardoBranco, Giovana SouzaPinheiro, João Paulo SilvaGodoi, Filipe Guilherme Andrade deMoreira, Renata GuimarãesDRUGSGENOTOXICITYLIPOPEROXIDATIONOXIDATIVE STRESSWATER POLLUTIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Although concentrations of pharmaceutical compounds in aquatic ecosystems are low, they can cause toxic effects on organisms. The aim of this study was to evaluate the effects of diclofenac (DCF), a non-steroidal anti-inflammatory drug, and caffeine (CAF), a central nervous system stimulant, both alone or combined, in Astyanax altiparanae males under acute exposure (96 h), measuring neurotoxicity biomarkers, antioxidant response and damage at biochemical and cellular levels. DCF concentration in water, separated and combined, was 3.08 mg L−1 and that of CAF was 9.59 mg L−1. To assess neurotoxicity, brain and muscle acetylcholinesterase (AChE) activities were measured. To evaluate oxidative stress, the enzymatic activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and glutathione S-transferase (GST), as well as lipoperoxidation (LPO), were analyzed in liver and gills. Activity of hepatic cyclooxygenase (COX) was also evaluated. Genotoxicity was assessed in blood using comet assay and micronucleus test, as well as nuclear abnormalities. DCF and CAF, alone or combined, had neither effect on AChE activity, nor in the activity of SOD, CAT, GPx and GST in gills. In liver, DCF inhibited SOD and GPx activity, CAF inhibited CAT activity, the mixture inhibited SOD and GST activity; although only fish exposed to CAF showed increased hepatic LPO. Under these experimental conditions, no effect on COX activity was observed, nor cytotoxic and genotoxic damage. The most pronounced effects were caused by the drugs separately, since both compounds altered the enzymes, but only CAF triggered LPO, showing more harmful effects.Fil: Muñoz Peñuela, Marcela. Universidade de Sao Paulo; BrasilFil: Lo Nostro, Fabiana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; ArgentinaFil: Dal'Olio Gomes, Aline. Universidade de Sao Paulo; BrasilFil: Tolussi, Carlos Eduardo. Universidade Anhembi Morumbi; BrasilFil: Branco, Giovana Souza. Universidade de Sao Paulo; BrasilFil: Pinheiro, João Paulo Silva. Universidade de Sao Paulo; BrasilFil: Godoi, Filipe Guilherme Andrade de. Universidade de Sao Paulo; BrasilFil: Moreira, Renata Guimarães. Universidade de Sao Paulo; BrasilElsevier Science Inc.2021-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/181633Muñoz Peñuela, Marcela; Lo Nostro, Fabiana Laura; Dal'Olio Gomes, Aline; Tolussi, Carlos Eduardo; Branco, Giovana Souza; et al.; Diclofenac and caffeine inhibit hepatic antioxidant enzymes in the freshwater fish Astyanax altiparanae (Teleostei: Characiformes); Elsevier Science Inc.; Comparative Biochemistry And Physiology. Toxicology & Pharmacology; 240; 2-2021; 1-81532-0456CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1532045620302106info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cbpc.2020.108910info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:05Zoai:ri.conicet.gov.ar:11336/181633instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:05.916CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Diclofenac and caffeine inhibit hepatic antioxidant enzymes in the freshwater fish Astyanax altiparanae (Teleostei: Characiformes)
title Diclofenac and caffeine inhibit hepatic antioxidant enzymes in the freshwater fish Astyanax altiparanae (Teleostei: Characiformes)
spellingShingle Diclofenac and caffeine inhibit hepatic antioxidant enzymes in the freshwater fish Astyanax altiparanae (Teleostei: Characiformes)
Muñoz Peñuela, Marcela
DRUGS
GENOTOXICITY
LIPOPEROXIDATION
OXIDATIVE STRESS
WATER POLLUTION
title_short Diclofenac and caffeine inhibit hepatic antioxidant enzymes in the freshwater fish Astyanax altiparanae (Teleostei: Characiformes)
title_full Diclofenac and caffeine inhibit hepatic antioxidant enzymes in the freshwater fish Astyanax altiparanae (Teleostei: Characiformes)
title_fullStr Diclofenac and caffeine inhibit hepatic antioxidant enzymes in the freshwater fish Astyanax altiparanae (Teleostei: Characiformes)
title_full_unstemmed Diclofenac and caffeine inhibit hepatic antioxidant enzymes in the freshwater fish Astyanax altiparanae (Teleostei: Characiformes)
title_sort Diclofenac and caffeine inhibit hepatic antioxidant enzymes in the freshwater fish Astyanax altiparanae (Teleostei: Characiformes)
dc.creator.none.fl_str_mv Muñoz Peñuela, Marcela
Lo Nostro, Fabiana Laura
Dal'Olio Gomes, Aline
Tolussi, Carlos Eduardo
Branco, Giovana Souza
Pinheiro, João Paulo Silva
Godoi, Filipe Guilherme Andrade de
Moreira, Renata Guimarães
author Muñoz Peñuela, Marcela
author_facet Muñoz Peñuela, Marcela
Lo Nostro, Fabiana Laura
Dal'Olio Gomes, Aline
Tolussi, Carlos Eduardo
Branco, Giovana Souza
Pinheiro, João Paulo Silva
Godoi, Filipe Guilherme Andrade de
Moreira, Renata Guimarães
author_role author
author2 Lo Nostro, Fabiana Laura
Dal'Olio Gomes, Aline
Tolussi, Carlos Eduardo
Branco, Giovana Souza
Pinheiro, João Paulo Silva
Godoi, Filipe Guilherme Andrade de
Moreira, Renata Guimarães
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv DRUGS
GENOTOXICITY
LIPOPEROXIDATION
OXIDATIVE STRESS
WATER POLLUTION
topic DRUGS
GENOTOXICITY
LIPOPEROXIDATION
OXIDATIVE STRESS
WATER POLLUTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Although concentrations of pharmaceutical compounds in aquatic ecosystems are low, they can cause toxic effects on organisms. The aim of this study was to evaluate the effects of diclofenac (DCF), a non-steroidal anti-inflammatory drug, and caffeine (CAF), a central nervous system stimulant, both alone or combined, in Astyanax altiparanae males under acute exposure (96 h), measuring neurotoxicity biomarkers, antioxidant response and damage at biochemical and cellular levels. DCF concentration in water, separated and combined, was 3.08 mg L−1 and that of CAF was 9.59 mg L−1. To assess neurotoxicity, brain and muscle acetylcholinesterase (AChE) activities were measured. To evaluate oxidative stress, the enzymatic activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and glutathione S-transferase (GST), as well as lipoperoxidation (LPO), were analyzed in liver and gills. Activity of hepatic cyclooxygenase (COX) was also evaluated. Genotoxicity was assessed in blood using comet assay and micronucleus test, as well as nuclear abnormalities. DCF and CAF, alone or combined, had neither effect on AChE activity, nor in the activity of SOD, CAT, GPx and GST in gills. In liver, DCF inhibited SOD and GPx activity, CAF inhibited CAT activity, the mixture inhibited SOD and GST activity; although only fish exposed to CAF showed increased hepatic LPO. Under these experimental conditions, no effect on COX activity was observed, nor cytotoxic and genotoxic damage. The most pronounced effects were caused by the drugs separately, since both compounds altered the enzymes, but only CAF triggered LPO, showing more harmful effects.
Fil: Muñoz Peñuela, Marcela. Universidade de Sao Paulo; Brasil
Fil: Lo Nostro, Fabiana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina
Fil: Dal'Olio Gomes, Aline. Universidade de Sao Paulo; Brasil
Fil: Tolussi, Carlos Eduardo. Universidade Anhembi Morumbi; Brasil
Fil: Branco, Giovana Souza. Universidade de Sao Paulo; Brasil
Fil: Pinheiro, João Paulo Silva. Universidade de Sao Paulo; Brasil
Fil: Godoi, Filipe Guilherme Andrade de. Universidade de Sao Paulo; Brasil
Fil: Moreira, Renata Guimarães. Universidade de Sao Paulo; Brasil
description Although concentrations of pharmaceutical compounds in aquatic ecosystems are low, they can cause toxic effects on organisms. The aim of this study was to evaluate the effects of diclofenac (DCF), a non-steroidal anti-inflammatory drug, and caffeine (CAF), a central nervous system stimulant, both alone or combined, in Astyanax altiparanae males under acute exposure (96 h), measuring neurotoxicity biomarkers, antioxidant response and damage at biochemical and cellular levels. DCF concentration in water, separated and combined, was 3.08 mg L−1 and that of CAF was 9.59 mg L−1. To assess neurotoxicity, brain and muscle acetylcholinesterase (AChE) activities were measured. To evaluate oxidative stress, the enzymatic activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and glutathione S-transferase (GST), as well as lipoperoxidation (LPO), were analyzed in liver and gills. Activity of hepatic cyclooxygenase (COX) was also evaluated. Genotoxicity was assessed in blood using comet assay and micronucleus test, as well as nuclear abnormalities. DCF and CAF, alone or combined, had neither effect on AChE activity, nor in the activity of SOD, CAT, GPx and GST in gills. In liver, DCF inhibited SOD and GPx activity, CAF inhibited CAT activity, the mixture inhibited SOD and GST activity; although only fish exposed to CAF showed increased hepatic LPO. Under these experimental conditions, no effect on COX activity was observed, nor cytotoxic and genotoxic damage. The most pronounced effects were caused by the drugs separately, since both compounds altered the enzymes, but only CAF triggered LPO, showing more harmful effects.
publishDate 2021
dc.date.none.fl_str_mv 2021-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/181633
Muñoz Peñuela, Marcela; Lo Nostro, Fabiana Laura; Dal'Olio Gomes, Aline; Tolussi, Carlos Eduardo; Branco, Giovana Souza; et al.; Diclofenac and caffeine inhibit hepatic antioxidant enzymes in the freshwater fish Astyanax altiparanae (Teleostei: Characiformes); Elsevier Science Inc.; Comparative Biochemistry And Physiology. Toxicology & Pharmacology; 240; 2-2021; 1-8
1532-0456
CONICET Digital
CONICET
url http://hdl.handle.net/11336/181633
identifier_str_mv Muñoz Peñuela, Marcela; Lo Nostro, Fabiana Laura; Dal'Olio Gomes, Aline; Tolussi, Carlos Eduardo; Branco, Giovana Souza; et al.; Diclofenac and caffeine inhibit hepatic antioxidant enzymes in the freshwater fish Astyanax altiparanae (Teleostei: Characiformes); Elsevier Science Inc.; Comparative Biochemistry And Physiology. Toxicology & Pharmacology; 240; 2-2021; 1-8
1532-0456
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1532045620302106
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cbpc.2020.108910
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Inc.
publisher.none.fl_str_mv Elsevier Science Inc.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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