Calendula officinalis L. (Asteraceae) possess antioxidant properties on Fe2+-initiated peroxidation of rat brain microsomes

Autores
Palacios, Alejandro; Barberón, Javier; Leaden, Patricio; Zeinsteger, Pedro Adolfo
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this study the effects of Calendula officinalis L. (Asteraceae) extract (CO) on the polyunsaturated fatty acid composition, chemiluminescence and unsaturation index of microsomes isolated from brain rat, are presented. After incubation of microsomes in an ascorbate (0.4 mM)-Fe2+ (2.15 μM) system (180 min at 37 °C) it was observed that the total cpm/mg protein originated from light emission:chemiluminescence was lower in brain microsomes obtained from CO group compared to the control group (without extract supplementation). Moreover, it was observed that the addition of the extract reduced chemiluminescence -measured as total cpm- in a concentration dependent manner. The fatty acid composition of brain microsomes from control group was profoundly modified when subjected to non-enzymatic lipoperoxidation with a considerable decrease of arachidonic acid C20:4ω6 and docosahexaenoic acid C22:6ω3. As a consequence, the unsaturation index, a parameter based on the maximal rate of oxidation of specific fatty acids, was higher in the CO group compared to controls. The simultaneous analysis of unsaturation index, chemiluminescence and fatty acid composition indicate that CO may act as an antioxidant protecting rat brain microsomes from peroxidative damage.
Facultad de Ciencias Veterinarias
Materia
Ciencias Veterinarias
Chemiluminescence
Lipoperoxidation
Marigold
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/85835
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/85835
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Calendula officinalis L. (Asteraceae) possess antioxidant properties on Fe2+-initiated peroxidation of rat brain microsomesPalacios, AlejandroBarberón, JavierLeaden, PatricioZeinsteger, Pedro AdolfoCiencias VeterinariasChemiluminescenceLipoperoxidationMarigoldIn this study the effects of Calendula officinalis L. (Asteraceae) extract (CO) on the polyunsaturated fatty acid composition, chemiluminescence and unsaturation index of microsomes isolated from brain rat, are presented. After incubation of microsomes in an ascorbate (0.4 mM)-Fe2+ (2.15 μM) system (180 min at 37 °C) it was observed that the total cpm/mg protein originated from light emission:chemiluminescence was lower in brain microsomes obtained from CO group compared to the control group (without extract supplementation). Moreover, it was observed that the addition of the extract reduced chemiluminescence -measured as total cpm- in a concentration dependent manner. The fatty acid composition of brain microsomes from control group was profoundly modified when subjected to non-enzymatic lipoperoxidation with a considerable decrease of arachidonic acid C20:4ω6 and docosahexaenoic acid C22:6ω3. As a consequence, the unsaturation index, a parameter based on the maximal rate of oxidation of specific fatty acids, was higher in the CO group compared to controls. The simultaneous analysis of unsaturation index, chemiluminescence and fatty acid composition indicate that CO may act as an antioxidant protecting rat brain microsomes from peroxidative damage.Facultad de Ciencias Veterinarias2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf91-97http://sedici.unlp.edu.ar/handle/10915/85835enginfo:eu-repo/semantics/altIdentifier/issn/1848-7718info:eu-repo/semantics/altIdentifier/doi/10.5599/admet.4.2.251info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-15T11:08:40Zoai:sedici.unlp.edu.ar:10915/85835Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-15 11:08:40.917SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Calendula officinalis L. (Asteraceae) possess antioxidant properties on Fe2+-initiated peroxidation of rat brain microsomes
title Calendula officinalis L. (Asteraceae) possess antioxidant properties on Fe2+-initiated peroxidation of rat brain microsomes
spellingShingle Calendula officinalis L. (Asteraceae) possess antioxidant properties on Fe2+-initiated peroxidation of rat brain microsomes
Palacios, Alejandro
Ciencias Veterinarias
Chemiluminescence
Lipoperoxidation
Marigold
title_short Calendula officinalis L. (Asteraceae) possess antioxidant properties on Fe2+-initiated peroxidation of rat brain microsomes
title_full Calendula officinalis L. (Asteraceae) possess antioxidant properties on Fe2+-initiated peroxidation of rat brain microsomes
title_fullStr Calendula officinalis L. (Asteraceae) possess antioxidant properties on Fe2+-initiated peroxidation of rat brain microsomes
title_full_unstemmed Calendula officinalis L. (Asteraceae) possess antioxidant properties on Fe2+-initiated peroxidation of rat brain microsomes
title_sort Calendula officinalis L. (Asteraceae) possess antioxidant properties on Fe2+-initiated peroxidation of rat brain microsomes
dc.creator.none.fl_str_mv Palacios, Alejandro
Barberón, Javier
Leaden, Patricio
Zeinsteger, Pedro Adolfo
author Palacios, Alejandro
author_facet Palacios, Alejandro
Barberón, Javier
Leaden, Patricio
Zeinsteger, Pedro Adolfo
author_role author
author2 Barberón, Javier
Leaden, Patricio
Zeinsteger, Pedro Adolfo
author2_role author
author
author
dc.subject.none.fl_str_mv Ciencias Veterinarias
Chemiluminescence
Lipoperoxidation
Marigold
topic Ciencias Veterinarias
Chemiluminescence
Lipoperoxidation
Marigold
dc.description.none.fl_txt_mv In this study the effects of Calendula officinalis L. (Asteraceae) extract (CO) on the polyunsaturated fatty acid composition, chemiluminescence and unsaturation index of microsomes isolated from brain rat, are presented. After incubation of microsomes in an ascorbate (0.4 mM)-Fe2+ (2.15 μM) system (180 min at 37 °C) it was observed that the total cpm/mg protein originated from light emission:chemiluminescence was lower in brain microsomes obtained from CO group compared to the control group (without extract supplementation). Moreover, it was observed that the addition of the extract reduced chemiluminescence -measured as total cpm- in a concentration dependent manner. The fatty acid composition of brain microsomes from control group was profoundly modified when subjected to non-enzymatic lipoperoxidation with a considerable decrease of arachidonic acid C20:4ω6 and docosahexaenoic acid C22:6ω3. As a consequence, the unsaturation index, a parameter based on the maximal rate of oxidation of specific fatty acids, was higher in the CO group compared to controls. The simultaneous analysis of unsaturation index, chemiluminescence and fatty acid composition indicate that CO may act as an antioxidant protecting rat brain microsomes from peroxidative damage.
Facultad de Ciencias Veterinarias
description In this study the effects of Calendula officinalis L. (Asteraceae) extract (CO) on the polyunsaturated fatty acid composition, chemiluminescence and unsaturation index of microsomes isolated from brain rat, are presented. After incubation of microsomes in an ascorbate (0.4 mM)-Fe2+ (2.15 μM) system (180 min at 37 °C) it was observed that the total cpm/mg protein originated from light emission:chemiluminescence was lower in brain microsomes obtained from CO group compared to the control group (without extract supplementation). Moreover, it was observed that the addition of the extract reduced chemiluminescence -measured as total cpm- in a concentration dependent manner. The fatty acid composition of brain microsomes from control group was profoundly modified when subjected to non-enzymatic lipoperoxidation with a considerable decrease of arachidonic acid C20:4ω6 and docosahexaenoic acid C22:6ω3. As a consequence, the unsaturation index, a parameter based on the maximal rate of oxidation of specific fatty acids, was higher in the CO group compared to controls. The simultaneous analysis of unsaturation index, chemiluminescence and fatty acid composition indicate that CO may act as an antioxidant protecting rat brain microsomes from peroxidative damage.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/85835
url http://sedici.unlp.edu.ar/handle/10915/85835
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1848-7718
info:eu-repo/semantics/altIdentifier/doi/10.5599/admet.4.2.251
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
91-97
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.22299

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