Vitamin A inhibits lipoperoxidation ascorbate-Fe⁺⁺ dependent of rat kidney microsomes and mitochondria

Autores
Piergiacomi, Viviana Angélica; Palacios, Alejandro; Catalá, Ángel
Año de publicación
1996
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In the present study it was investigated if Vitamin A supplementation could protect rat kidney microsomes and mitochondria from in vitro lipoperoxidation. After incubation of rat kidney microsomes and mitochondria in an ascorbate-Fe⁺⁺ system, at 37°C during 60 min, it was observed that the total cpm/mg protein originated from light emission (chemiluminescence) was lower in those organelles obtained from the control group when compared with the vitamin A supplemented group. The fatty acid composition of microsomes and mitochondria from control group was profoundly modified when subjected to nonenzymatic lipoperoxidation with a considerable decrease of arachidonic acid, C20:4 (n-6) and docosapentaenoic acid, C22:5 (n−3) in mitochondria and docosahexaenoic acid C22:6 (n-3) in microsomes.As a consequence the peroxidizability index, a parameter based on the maximal rate of oxidation of specific fatty acids was higher in the supplemented animals than in those used as control. These results indicate that Vitamin A may act as antioxidant protecting rat kidney microsomes and mitochondria from deleterious effect.
Facultad de Ciencias Veterinarias
Materia
Bioquímica
Lipoperoxidation
Microsomes
Mitochondria
Rat kidney
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/145820
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/145820
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Vitamin A inhibits lipoperoxidation ascorbate-Fe⁺⁺ dependent of rat kidney microsomes and mitochondriaPiergiacomi, Viviana AngélicaPalacios, AlejandroCatalá, ÁngelBioquímicaLipoperoxidationMicrosomesMitochondriaRat kidneyIn the present study it was investigated if Vitamin A supplementation could protect rat kidney microsomes and mitochondria from in vitro lipoperoxidation. After incubation of rat kidney microsomes and mitochondria in an ascorbate-Fe⁺⁺ system, at 37°C during 60 min, it was observed that the total cpm/mg protein originated from light emission (chemiluminescence) was lower in those organelles obtained from the control group when compared with the vitamin A supplemented group. The fatty acid composition of microsomes and mitochondria from control group was profoundly modified when subjected to nonenzymatic lipoperoxidation with a considerable decrease of arachidonic acid, C20:4 (n-6) and docosapentaenoic acid, C22:5 (n−3) in mitochondria and docosahexaenoic acid C22:6 (n-3) in microsomes.As a consequence the peroxidizability index, a parameter based on the maximal rate of oxidation of specific fatty acids was higher in the supplemented animals than in those used as control. These results indicate that Vitamin A may act as antioxidant protecting rat kidney microsomes and mitochondria from deleterious effect.Facultad de Ciencias Veterinarias1996-12-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf121-125http://sedici.unlp.edu.ar/handle/10915/145820enginfo:eu-repo/semantics/altIdentifier/issn/0300-8177info:eu-repo/semantics/altIdentifier/issn/1573-4919info:eu-repo/semantics/altIdentifier/doi/10.1007/bf00229473info:eu-repo/semantics/altIdentifier/pmid/8979260info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-17T10:15:05Zoai:sedici.unlp.edu.ar:10915/145820Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-17 10:15:05.664SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Vitamin A inhibits lipoperoxidation ascorbate-Fe⁺⁺ dependent of rat kidney microsomes and mitochondria
title Vitamin A inhibits lipoperoxidation ascorbate-Fe⁺⁺ dependent of rat kidney microsomes and mitochondria
spellingShingle Vitamin A inhibits lipoperoxidation ascorbate-Fe⁺⁺ dependent of rat kidney microsomes and mitochondria
Piergiacomi, Viviana Angélica
Bioquímica
Lipoperoxidation
Microsomes
Mitochondria
Rat kidney
title_short Vitamin A inhibits lipoperoxidation ascorbate-Fe⁺⁺ dependent of rat kidney microsomes and mitochondria
title_full Vitamin A inhibits lipoperoxidation ascorbate-Fe⁺⁺ dependent of rat kidney microsomes and mitochondria
title_fullStr Vitamin A inhibits lipoperoxidation ascorbate-Fe⁺⁺ dependent of rat kidney microsomes and mitochondria
title_full_unstemmed Vitamin A inhibits lipoperoxidation ascorbate-Fe⁺⁺ dependent of rat kidney microsomes and mitochondria
title_sort Vitamin A inhibits lipoperoxidation ascorbate-Fe⁺⁺ dependent of rat kidney microsomes and mitochondria
dc.creator.none.fl_str_mv Piergiacomi, Viviana Angélica
Palacios, Alejandro
Catalá, Ángel
author Piergiacomi, Viviana Angélica
author_facet Piergiacomi, Viviana Angélica
Palacios, Alejandro
Catalá, Ángel
author_role author
author2 Palacios, Alejandro
Catalá, Ángel
author2_role author
author
dc.subject.none.fl_str_mv Bioquímica
Lipoperoxidation
Microsomes
Mitochondria
Rat kidney
topic Bioquímica
Lipoperoxidation
Microsomes
Mitochondria
Rat kidney
dc.description.none.fl_txt_mv In the present study it was investigated if Vitamin A supplementation could protect rat kidney microsomes and mitochondria from in vitro lipoperoxidation. After incubation of rat kidney microsomes and mitochondria in an ascorbate-Fe⁺⁺ system, at 37°C during 60 min, it was observed that the total cpm/mg protein originated from light emission (chemiluminescence) was lower in those organelles obtained from the control group when compared with the vitamin A supplemented group. The fatty acid composition of microsomes and mitochondria from control group was profoundly modified when subjected to nonenzymatic lipoperoxidation with a considerable decrease of arachidonic acid, C20:4 (n-6) and docosapentaenoic acid, C22:5 (n−3) in mitochondria and docosahexaenoic acid C22:6 (n-3) in microsomes.As a consequence the peroxidizability index, a parameter based on the maximal rate of oxidation of specific fatty acids was higher in the supplemented animals than in those used as control. These results indicate that Vitamin A may act as antioxidant protecting rat kidney microsomes and mitochondria from deleterious effect.
Facultad de Ciencias Veterinarias
description In the present study it was investigated if Vitamin A supplementation could protect rat kidney microsomes and mitochondria from in vitro lipoperoxidation. After incubation of rat kidney microsomes and mitochondria in an ascorbate-Fe⁺⁺ system, at 37°C during 60 min, it was observed that the total cpm/mg protein originated from light emission (chemiluminescence) was lower in those organelles obtained from the control group when compared with the vitamin A supplemented group. The fatty acid composition of microsomes and mitochondria from control group was profoundly modified when subjected to nonenzymatic lipoperoxidation with a considerable decrease of arachidonic acid, C20:4 (n-6) and docosapentaenoic acid, C22:5 (n−3) in mitochondria and docosahexaenoic acid C22:6 (n-3) in microsomes.As a consequence the peroxidizability index, a parameter based on the maximal rate of oxidation of specific fatty acids was higher in the supplemented animals than in those used as control. These results indicate that Vitamin A may act as antioxidant protecting rat kidney microsomes and mitochondria from deleterious effect.
publishDate 1996
dc.date.none.fl_str_mv 1996-12-20
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/145820
url http://sedici.unlp.edu.ar/handle/10915/145820
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0300-8177
info:eu-repo/semantics/altIdentifier/issn/1573-4919
info:eu-repo/semantics/altIdentifier/doi/10.1007/bf00229473
info:eu-repo/semantics/altIdentifier/pmid/8979260
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
121-125
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.000565

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