Intratracheal instillation of lipopolymeric vectors and the effect on mice lung physiology

Autores
Xisto, Debora G.; Temprana, Carlos Facundo; Martini, Sabrina V.; Silva, Adriana L.; Abreu, Soraia G.; Silva, Johnatas D.; Crosseti, Julia; Rocco, Patricia R. M.; Alonso, Silvia del Valle; Morales, Marcelo M.
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background/Aims: The current study compared the effects of intratracheal administration of different lipopolymeric vectors on lung function and histology in normal mice. Methods: Forty-eight BALB/c mice were randomly divided into 8 groups (6/group). All animals received intratracheal instillation of the following suspensions: polymerized [(A) 1,2-dimyristoyl-sn-glycero-3- phosphocholine (DMPC):1,2-bis-(tricosa-10,12-diynoyl)-sn-glycero-3- phosphocholine (DC8,9PC):1,2-dioleoyl-3-trimethylammonium-propane (DOTAP), (B) DMPC:DC8,9PC:stearylamine (SA), (C) DMPC:DC8,9PC:myristoylcholine chloride (MCl)]; nonpolymerized [(D) DMPC:DC8,9PC:DOTAP, (E) DMPC:DC8,9PC:SA, (F) DMPC:DC8,9PC:MCl] together with plasmid DNA; vehicle (control), and pDsRed2-N1 plasmid DNA (DNA). At 24 h, the survival rate, lung mechanics (resistive and viscoelastic pressure, static elastance) and morphometry were analyzed. Results: The survival rate was 50% in D, 40% in E and F, and 100% in the CTRL, DNA, A, B and C groups. Animals from groups D, E, and F that died presented diffuse pulmonary hemorrhagic capillaritis. Lung mechanics, the fraction of normal and collapsed alveoli, as well as the number of polymorphonuclear and mononuclear cells in lung tissue were similar in all surviving mice. Conclusion: Intratracheal instillation of polymerized particles is safe compared with nonpolymerized formulations and may be used for future gene/drug therapy.
Fil: Xisto, Debora G.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Temprana, Carlos Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Biomembranas; Argentina
Fil: Martini, Sabrina V.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Silva, Adriana L.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Abreu, Soraia G.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Silva, Johnatas D.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Crosseti, Julia. Universidade Federal do Rio de Janeiro; Brasil
Fil: Rocco, Patricia R. M.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Alonso, Silvia del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Biomembranas; Argentina
Fil: Morales, Marcelo M.. Universidade Federal do Rio de Janeiro; Brasil
Materia
Gene Therapy
Lipopolymers
Lung
Mechanics
Mice
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/75978
Acceder
id CONICETDig_3dd9812ef09b4a7db345626402eacbaf
oai_identifier_str oai:ri.conicet.gov.ar:11336/75978
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Intratracheal instillation of lipopolymeric vectors and the effect on mice lung physiologyXisto, Debora G.Temprana, Carlos FacundoMartini, Sabrina V.Silva, Adriana L.Abreu, Soraia G.Silva, Johnatas D.Crosseti, JuliaRocco, Patricia R. M.Alonso, Silvia del ValleMorales, Marcelo M.Gene TherapyLipopolymersLungMechanicsMicehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Background/Aims: The current study compared the effects of intratracheal administration of different lipopolymeric vectors on lung function and histology in normal mice. Methods: Forty-eight BALB/c mice were randomly divided into 8 groups (6/group). All animals received intratracheal instillation of the following suspensions: polymerized [(A) 1,2-dimyristoyl-sn-glycero-3- phosphocholine (DMPC):1,2-bis-(tricosa-10,12-diynoyl)-sn-glycero-3- phosphocholine (DC8,9PC):1,2-dioleoyl-3-trimethylammonium-propane (DOTAP), (B) DMPC:DC8,9PC:stearylamine (SA), (C) DMPC:DC8,9PC:myristoylcholine chloride (MCl)]; nonpolymerized [(D) DMPC:DC8,9PC:DOTAP, (E) DMPC:DC8,9PC:SA, (F) DMPC:DC8,9PC:MCl] together with plasmid DNA; vehicle (control), and pDsRed2-N1 plasmid DNA (DNA). At 24 h, the survival rate, lung mechanics (resistive and viscoelastic pressure, static elastance) and morphometry were analyzed. Results: The survival rate was 50% in D, 40% in E and F, and 100% in the CTRL, DNA, A, B and C groups. Animals from groups D, E, and F that died presented diffuse pulmonary hemorrhagic capillaritis. Lung mechanics, the fraction of normal and collapsed alveoli, as well as the number of polymorphonuclear and mononuclear cells in lung tissue were similar in all surviving mice. Conclusion: Intratracheal instillation of polymerized particles is safe compared with nonpolymerized formulations and may be used for future gene/drug therapy.Fil: Xisto, Debora G.. Universidade Federal do Rio de Janeiro; BrasilFil: Temprana, Carlos Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Biomembranas; ArgentinaFil: Martini, Sabrina V.. Universidade Federal do Rio de Janeiro; BrasilFil: Silva, Adriana L.. Universidade Federal do Rio de Janeiro; BrasilFil: Abreu, Soraia G.. Universidade Federal do Rio de Janeiro; BrasilFil: Silva, Johnatas D.. Universidade Federal do Rio de Janeiro; BrasilFil: Crosseti, Julia. Universidade Federal do Rio de Janeiro; BrasilFil: Rocco, Patricia R. M.. Universidade Federal do Rio de Janeiro; BrasilFil: Alonso, Silvia del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Biomembranas; ArgentinaFil: Morales, Marcelo M.. Universidade Federal do Rio de Janeiro; BrasilKarger2012-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/75978Xisto, Debora G.; Temprana, Carlos Facundo; Martini, Sabrina V.; Silva, Adriana L.; Abreu, Soraia G.; et al.; Intratracheal instillation of lipopolymeric vectors and the effect on mice lung physiology; Karger; Cellular Physiology and Biochemistry; 29; 5-6; 3-2012; 791-7981015-8987CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1159/000336917info:eu-repo/semantics/altIdentifier/url/https://www.karger.com/Article/Abstract/336917info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-01-08T13:04:02Zoai:ri.conicet.gov.ar:11336/75978instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-01-08 13:04:02.786CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Intratracheal instillation of lipopolymeric vectors and the effect on mice lung physiology
title Intratracheal instillation of lipopolymeric vectors and the effect on mice lung physiology
spellingShingle Intratracheal instillation of lipopolymeric vectors and the effect on mice lung physiology
Xisto, Debora G.
Gene Therapy
Lipopolymers
Lung
Mechanics
Mice
title_short Intratracheal instillation of lipopolymeric vectors and the effect on mice lung physiology
title_full Intratracheal instillation of lipopolymeric vectors and the effect on mice lung physiology
title_fullStr Intratracheal instillation of lipopolymeric vectors and the effect on mice lung physiology
title_full_unstemmed Intratracheal instillation of lipopolymeric vectors and the effect on mice lung physiology
title_sort Intratracheal instillation of lipopolymeric vectors and the effect on mice lung physiology
dc.creator.none.fl_str_mv Xisto, Debora G.
Temprana, Carlos Facundo
Martini, Sabrina V.
Silva, Adriana L.
Abreu, Soraia G.
Silva, Johnatas D.
Crosseti, Julia
Rocco, Patricia R. M.
Alonso, Silvia del Valle
Morales, Marcelo M.
author Xisto, Debora G.
author_facet Xisto, Debora G.
Temprana, Carlos Facundo
Martini, Sabrina V.
Silva, Adriana L.
Abreu, Soraia G.
Silva, Johnatas D.
Crosseti, Julia
Rocco, Patricia R. M.
Alonso, Silvia del Valle
Morales, Marcelo M.
author_role author
author2 Temprana, Carlos Facundo
Martini, Sabrina V.
Silva, Adriana L.
Abreu, Soraia G.
Silva, Johnatas D.
Crosseti, Julia
Rocco, Patricia R. M.
Alonso, Silvia del Valle
Morales, Marcelo M.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Gene Therapy
Lipopolymers
Lung
Mechanics
Mice
topic Gene Therapy
Lipopolymers
Lung
Mechanics
Mice
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background/Aims: The current study compared the effects of intratracheal administration of different lipopolymeric vectors on lung function and histology in normal mice. Methods: Forty-eight BALB/c mice were randomly divided into 8 groups (6/group). All animals received intratracheal instillation of the following suspensions: polymerized [(A) 1,2-dimyristoyl-sn-glycero-3- phosphocholine (DMPC):1,2-bis-(tricosa-10,12-diynoyl)-sn-glycero-3- phosphocholine (DC8,9PC):1,2-dioleoyl-3-trimethylammonium-propane (DOTAP), (B) DMPC:DC8,9PC:stearylamine (SA), (C) DMPC:DC8,9PC:myristoylcholine chloride (MCl)]; nonpolymerized [(D) DMPC:DC8,9PC:DOTAP, (E) DMPC:DC8,9PC:SA, (F) DMPC:DC8,9PC:MCl] together with plasmid DNA; vehicle (control), and pDsRed2-N1 plasmid DNA (DNA). At 24 h, the survival rate, lung mechanics (resistive and viscoelastic pressure, static elastance) and morphometry were analyzed. Results: The survival rate was 50% in D, 40% in E and F, and 100% in the CTRL, DNA, A, B and C groups. Animals from groups D, E, and F that died presented diffuse pulmonary hemorrhagic capillaritis. Lung mechanics, the fraction of normal and collapsed alveoli, as well as the number of polymorphonuclear and mononuclear cells in lung tissue were similar in all surviving mice. Conclusion: Intratracheal instillation of polymerized particles is safe compared with nonpolymerized formulations and may be used for future gene/drug therapy.
Fil: Xisto, Debora G.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Temprana, Carlos Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Biomembranas; Argentina
Fil: Martini, Sabrina V.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Silva, Adriana L.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Abreu, Soraia G.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Silva, Johnatas D.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Crosseti, Julia. Universidade Federal do Rio de Janeiro; Brasil
Fil: Rocco, Patricia R. M.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Alonso, Silvia del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Biomembranas; Argentina
Fil: Morales, Marcelo M.. Universidade Federal do Rio de Janeiro; Brasil
description Background/Aims: The current study compared the effects of intratracheal administration of different lipopolymeric vectors on lung function and histology in normal mice. Methods: Forty-eight BALB/c mice were randomly divided into 8 groups (6/group). All animals received intratracheal instillation of the following suspensions: polymerized [(A) 1,2-dimyristoyl-sn-glycero-3- phosphocholine (DMPC):1,2-bis-(tricosa-10,12-diynoyl)-sn-glycero-3- phosphocholine (DC8,9PC):1,2-dioleoyl-3-trimethylammonium-propane (DOTAP), (B) DMPC:DC8,9PC:stearylamine (SA), (C) DMPC:DC8,9PC:myristoylcholine chloride (MCl)]; nonpolymerized [(D) DMPC:DC8,9PC:DOTAP, (E) DMPC:DC8,9PC:SA, (F) DMPC:DC8,9PC:MCl] together with plasmid DNA; vehicle (control), and pDsRed2-N1 plasmid DNA (DNA). At 24 h, the survival rate, lung mechanics (resistive and viscoelastic pressure, static elastance) and morphometry were analyzed. Results: The survival rate was 50% in D, 40% in E and F, and 100% in the CTRL, DNA, A, B and C groups. Animals from groups D, E, and F that died presented diffuse pulmonary hemorrhagic capillaritis. Lung mechanics, the fraction of normal and collapsed alveoli, as well as the number of polymorphonuclear and mononuclear cells in lung tissue were similar in all surviving mice. Conclusion: Intratracheal instillation of polymerized particles is safe compared with nonpolymerized formulations and may be used for future gene/drug therapy.
publishDate 2012
dc.date.none.fl_str_mv 2012-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/75978
Xisto, Debora G.; Temprana, Carlos Facundo; Martini, Sabrina V.; Silva, Adriana L.; Abreu, Soraia G.; et al.; Intratracheal instillation of lipopolymeric vectors and the effect on mice lung physiology; Karger; Cellular Physiology and Biochemistry; 29; 5-6; 3-2012; 791-798
1015-8987
CONICET Digital
CONICET
url http://hdl.handle.net/11336/75978
identifier_str_mv Xisto, Debora G.; Temprana, Carlos Facundo; Martini, Sabrina V.; Silva, Adriana L.; Abreu, Soraia G.; et al.; Intratracheal instillation of lipopolymeric vectors and the effect on mice lung physiology; Karger; Cellular Physiology and Biochemistry; 29; 5-6; 3-2012; 791-798
1015-8987
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1159/000336917
info:eu-repo/semantics/altIdentifier/url/https://www.karger.com/Article/Abstract/336917
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Karger
publisher.none.fl_str_mv Karger
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1853775684248272896
score 12.747614

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