Biphasic Modulation of Neutrophil Migration by Aqueous Extracts of Uncaria tomentosa in Murine Lung

Autores
Roque, Noelia; Cremonezzi, David César; Bigliani, María Celeste; Grondona, Ezequiel; Zunino, María Paula; Ponce, Andrés Alberto
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In the history of medicine, the treatment of disease has been influenced by an intimate contact with nature. A mice model of lipopolysaccharide (LPS)-induced acute lung injury was used to evaluate the protective effects of long-term water-soluble administration ad libitum of Uncaria tomentosa extracts (20 gr/L; UTE) in lung inflammation. Swiss mice had LPS (1,67µg/ml) instilled intranasally 3hs before sacrificed, and were then pre-treated with UTE for 7, 15, 30 or 90 days or with a single dose of dexamethasone (2,5 mg/kg, DX). Inflammatory cell concentration was measured in the bronchoalveolar fluid (BALF) and histology was performed. No acute or chronic toxicity signs were observed in the clinical status. In addition, body weight, food consumption, organ weight, kidney, liver, and lung pathology were not found to be affected by the UTE treatments. UTE or DX significantly reduced the lung edema, exudation and lung injury histology for 7 and 90 day treatments. In addition, pre-treatment with UTE revealed a biphasic attenuated recruitment in BALF from neutrophils at 7 and 90 days induced by endotoxin exposure compared to the control (p<0.05). These data suggest that UTE initially induces a nonspecific response that is transient protection from PMNs migration into the lung mice.
Fil: Roque, Noelia. Universidad Nacional de Córdoba; Argentina
Fil: Cremonezzi, David César. Universidad Nacional de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bigliani, María Celeste. Universidad Nacional de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Grondona, Ezequiel. Universidad Nacional de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zunino, María Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Ponce, Andrés Alberto. Universidad Nacional de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Cystic Fibrosis
Uncaria Tomentosa
Lung
Mice
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/24333
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/24333
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Biphasic Modulation of Neutrophil Migration by Aqueous Extracts of Uncaria tomentosa in Murine LungRoque, NoeliaCremonezzi, David CésarBigliani, María CelesteGrondona, EzequielZunino, María PaulaPonce, Andrés AlbertoCystic FibrosisUncaria TomentosaLungMicehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3In the history of medicine, the treatment of disease has been influenced by an intimate contact with nature. A mice model of lipopolysaccharide (LPS)-induced acute lung injury was used to evaluate the protective effects of long-term water-soluble administration ad libitum of Uncaria tomentosa extracts (20 gr/L; UTE) in lung inflammation. Swiss mice had LPS (1,67µg/ml) instilled intranasally 3hs before sacrificed, and were then pre-treated with UTE for 7, 15, 30 or 90 days or with a single dose of dexamethasone (2,5 mg/kg, DX). Inflammatory cell concentration was measured in the bronchoalveolar fluid (BALF) and histology was performed. No acute or chronic toxicity signs were observed in the clinical status. In addition, body weight, food consumption, organ weight, kidney, liver, and lung pathology were not found to be affected by the UTE treatments. UTE or DX significantly reduced the lung edema, exudation and lung injury histology for 7 and 90 day treatments. In addition, pre-treatment with UTE revealed a biphasic attenuated recruitment in BALF from neutrophils at 7 and 90 days induced by endotoxin exposure compared to the control (p<0.05). These data suggest that UTE initially induces a nonspecific response that is transient protection from PMNs migration into the lung mice.Fil: Roque, Noelia. Universidad Nacional de Córdoba; ArgentinaFil: Cremonezzi, David César. Universidad Nacional de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bigliani, María Celeste. Universidad Nacional de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Grondona, Ezequiel. Universidad Nacional de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Zunino, María Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Ponce, Andrés Alberto. Universidad Nacional de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaDe Gruyter2009-04-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24333Roque, Noelia; Cremonezzi, David César; Bigliani, María Celeste; Grondona, Ezequiel; Zunino, María Paula; et al.; Biphasic Modulation of Neutrophil Migration by Aqueous Extracts of Uncaria tomentosa in Murine Lung ; De Gruyter; Journal of Complementary and Integrative Medicine; 6; 1; 24-4-2009; 1222-12371553-3840CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.2202/1553-3840.1222info:eu-repo/semantics/altIdentifier/url/https://www.degruyter.com/view/j/jcim.2009.6.1/jcim.2009.6.1.1222/jcim.2009.6.1.1222.xmlinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:35:00Zoai:ri.conicet.gov.ar:11336/24333instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:35:00.911CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Biphasic Modulation of Neutrophil Migration by Aqueous Extracts of Uncaria tomentosa in Murine Lung
title Biphasic Modulation of Neutrophil Migration by Aqueous Extracts of Uncaria tomentosa in Murine Lung
spellingShingle Biphasic Modulation of Neutrophil Migration by Aqueous Extracts of Uncaria tomentosa in Murine Lung
Roque, Noelia
Cystic Fibrosis
Uncaria Tomentosa
Lung
Mice
title_short Biphasic Modulation of Neutrophil Migration by Aqueous Extracts of Uncaria tomentosa in Murine Lung
title_full Biphasic Modulation of Neutrophil Migration by Aqueous Extracts of Uncaria tomentosa in Murine Lung
title_fullStr Biphasic Modulation of Neutrophil Migration by Aqueous Extracts of Uncaria tomentosa in Murine Lung
title_full_unstemmed Biphasic Modulation of Neutrophil Migration by Aqueous Extracts of Uncaria tomentosa in Murine Lung
title_sort Biphasic Modulation of Neutrophil Migration by Aqueous Extracts of Uncaria tomentosa in Murine Lung
dc.creator.none.fl_str_mv Roque, Noelia
Cremonezzi, David César
Bigliani, María Celeste
Grondona, Ezequiel
Zunino, María Paula
Ponce, Andrés Alberto
author Roque, Noelia
author_facet Roque, Noelia
Cremonezzi, David César
Bigliani, María Celeste
Grondona, Ezequiel
Zunino, María Paula
Ponce, Andrés Alberto
author_role author
author2 Cremonezzi, David César
Bigliani, María Celeste
Grondona, Ezequiel
Zunino, María Paula
Ponce, Andrés Alberto
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Cystic Fibrosis
Uncaria Tomentosa
Lung
Mice
topic Cystic Fibrosis
Uncaria Tomentosa
Lung
Mice
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv In the history of medicine, the treatment of disease has been influenced by an intimate contact with nature. A mice model of lipopolysaccharide (LPS)-induced acute lung injury was used to evaluate the protective effects of long-term water-soluble administration ad libitum of Uncaria tomentosa extracts (20 gr/L; UTE) in lung inflammation. Swiss mice had LPS (1,67µg/ml) instilled intranasally 3hs before sacrificed, and were then pre-treated with UTE for 7, 15, 30 or 90 days or with a single dose of dexamethasone (2,5 mg/kg, DX). Inflammatory cell concentration was measured in the bronchoalveolar fluid (BALF) and histology was performed. No acute or chronic toxicity signs were observed in the clinical status. In addition, body weight, food consumption, organ weight, kidney, liver, and lung pathology were not found to be affected by the UTE treatments. UTE or DX significantly reduced the lung edema, exudation and lung injury histology for 7 and 90 day treatments. In addition, pre-treatment with UTE revealed a biphasic attenuated recruitment in BALF from neutrophils at 7 and 90 days induced by endotoxin exposure compared to the control (p<0.05). These data suggest that UTE initially induces a nonspecific response that is transient protection from PMNs migration into the lung mice.
Fil: Roque, Noelia. Universidad Nacional de Córdoba; Argentina
Fil: Cremonezzi, David César. Universidad Nacional de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bigliani, María Celeste. Universidad Nacional de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Grondona, Ezequiel. Universidad Nacional de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zunino, María Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Ponce, Andrés Alberto. Universidad Nacional de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description In the history of medicine, the treatment of disease has been influenced by an intimate contact with nature. A mice model of lipopolysaccharide (LPS)-induced acute lung injury was used to evaluate the protective effects of long-term water-soluble administration ad libitum of Uncaria tomentosa extracts (20 gr/L; UTE) in lung inflammation. Swiss mice had LPS (1,67µg/ml) instilled intranasally 3hs before sacrificed, and were then pre-treated with UTE for 7, 15, 30 or 90 days or with a single dose of dexamethasone (2,5 mg/kg, DX). Inflammatory cell concentration was measured in the bronchoalveolar fluid (BALF) and histology was performed. No acute or chronic toxicity signs were observed in the clinical status. In addition, body weight, food consumption, organ weight, kidney, liver, and lung pathology were not found to be affected by the UTE treatments. UTE or DX significantly reduced the lung edema, exudation and lung injury histology for 7 and 90 day treatments. In addition, pre-treatment with UTE revealed a biphasic attenuated recruitment in BALF from neutrophils at 7 and 90 days induced by endotoxin exposure compared to the control (p<0.05). These data suggest that UTE initially induces a nonspecific response that is transient protection from PMNs migration into the lung mice.
publishDate 2009
dc.date.none.fl_str_mv 2009-04-24
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/24333
Roque, Noelia; Cremonezzi, David César; Bigliani, María Celeste; Grondona, Ezequiel; Zunino, María Paula; et al.; Biphasic Modulation of Neutrophil Migration by Aqueous Extracts of Uncaria tomentosa in Murine Lung ; De Gruyter; Journal of Complementary and Integrative Medicine; 6; 1; 24-4-2009; 1222-1237
1553-3840
CONICET Digital
CONICET
url http://hdl.handle.net/11336/24333
identifier_str_mv Roque, Noelia; Cremonezzi, David César; Bigliani, María Celeste; Grondona, Ezequiel; Zunino, María Paula; et al.; Biphasic Modulation of Neutrophil Migration by Aqueous Extracts of Uncaria tomentosa in Murine Lung ; De Gruyter; Journal of Complementary and Integrative Medicine; 6; 1; 24-4-2009; 1222-1237
1553-3840
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.2202/1553-3840.1222
info:eu-repo/semantics/altIdentifier/url/https://www.degruyter.com/view/j/jcim.2009.6.1/jcim.2009.6.1.1222/jcim.2009.6.1.1222.xml
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv De Gruyter
publisher.none.fl_str_mv De Gruyter
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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