Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1

Autores
Juarez, Antonio; Villa Guerrero, Juan Angel; Lanza, Val F.; Lázaro, Beatriz; de la Cruz, Fernando; Alvarez, Hector Manuel; Moncalián, Gabriel
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Rhodococcus jostii RHA1 and other actinobacteria accumulate triglycerides (TAG) under nutrient starvation. This property has an important biotechnological potential in the production of sustainable oils. Results: To gain insight into the metabolic pathways involved in TAG accumulation, we analysed the transcriptome of R jostii RHA1 under nutrient-limiting conditions. We correlate these physiological conditions with significant changes in cell physiology. The main consequence was a global switch from catabolic to anabolic pathways. Interestingly, the Entner-Doudoroff (ED) pathway was upregulated in detriment of the glycolysis or pentose phosphate pathways. ED induction was independent of the carbon source (either gluconate or glucose). Some of the diacylglycerol acyltransferase genes involved in the last step of the Kennedy pathway were also upregulated. A common feature of the promoter region of most upregulated genes was the presence of a consensus binding sequence for the cAMP-dependent CRP regulator. Conclusion: This is the first experimental observation of an ED shift under nutrient starvation conditions. Knowledge of this switch could help in the design of metabolomic approaches to optimize carbon derivation for single cell oil production.
Fil: Juarez, Antonio. Institut de Bioenginyeria de Catalunya; España
Fil: Villa Guerrero, Juan Angel. Universidad de Cantabria; España
Fil: Lanza, Val F.. Universidad de Cantabria; España
Fil: Lázaro, Beatriz. Universidad de Cantabria; España
Fil: de la Cruz, Fernando. Universidad de Cantabria; España
Fil: Alvarez, Hector Manuel. Universidad Nacional de la Patagonia ; Argentina
Fil: Moncalián, Gabriel. Universidad de Cantabria; España
Materia
CRP
ENTNER-DOUDOROFF PATHWAY
NUTRIENT STARVATION
RHODOCOCCUS
RNA-SEQ
TRIACYLGLYCEROL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/36941
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/36941
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1Juarez, AntonioVilla Guerrero, Juan AngelLanza, Val F.Lázaro, Beatrizde la Cruz, FernandoAlvarez, Hector ManuelMoncalián, GabrielCRPENTNER-DOUDOROFF PATHWAYNUTRIENT STARVATIONRHODOCOCCUSRNA-SEQTRIACYLGLYCEROLhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: Rhodococcus jostii RHA1 and other actinobacteria accumulate triglycerides (TAG) under nutrient starvation. This property has an important biotechnological potential in the production of sustainable oils. Results: To gain insight into the metabolic pathways involved in TAG accumulation, we analysed the transcriptome of R jostii RHA1 under nutrient-limiting conditions. We correlate these physiological conditions with significant changes in cell physiology. The main consequence was a global switch from catabolic to anabolic pathways. Interestingly, the Entner-Doudoroff (ED) pathway was upregulated in detriment of the glycolysis or pentose phosphate pathways. ED induction was independent of the carbon source (either gluconate or glucose). Some of the diacylglycerol acyltransferase genes involved in the last step of the Kennedy pathway were also upregulated. A common feature of the promoter region of most upregulated genes was the presence of a consensus binding sequence for the cAMP-dependent CRP regulator. Conclusion: This is the first experimental observation of an ED shift under nutrient starvation conditions. Knowledge of this switch could help in the design of metabolomic approaches to optimize carbon derivation for single cell oil production.Fil: Juarez, Antonio. Institut de Bioenginyeria de Catalunya; EspañaFil: Villa Guerrero, Juan Angel. Universidad de Cantabria; EspañaFil: Lanza, Val F.. Universidad de Cantabria; EspañaFil: Lázaro, Beatriz. Universidad de Cantabria; EspañaFil: de la Cruz, Fernando. Universidad de Cantabria; EspañaFil: Alvarez, Hector Manuel. Universidad Nacional de la Patagonia ; ArgentinaFil: Moncalián, Gabriel. Universidad de Cantabria; EspañaBioMed Central2017-02-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/36941Juarez, Antonio; Villa Guerrero, Juan Angel; Lanza, Val F.; Lázaro, Beatriz; de la Cruz, Fernando; et al.; Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1; BioMed Central; Microbial Cell Factories; 16; 35; 27-2-2017; 1-121475-2859CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1186/s12934-017-0651-7info:eu-repo/semantics/altIdentifier/url/https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-017-0651-7info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:08:16Zoai:ri.conicet.gov.ar:11336/36941instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:08:16.263CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1
title Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1
spellingShingle Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1
Juarez, Antonio
CRP
ENTNER-DOUDOROFF PATHWAY
NUTRIENT STARVATION
RHODOCOCCUS
RNA-SEQ
TRIACYLGLYCEROL
title_short Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1
title_full Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1
title_fullStr Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1
title_full_unstemmed Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1
title_sort Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1
dc.creator.none.fl_str_mv Juarez, Antonio
Villa Guerrero, Juan Angel
Lanza, Val F.
Lázaro, Beatriz
de la Cruz, Fernando
Alvarez, Hector Manuel
Moncalián, Gabriel
author Juarez, Antonio
author_facet Juarez, Antonio
Villa Guerrero, Juan Angel
Lanza, Val F.
Lázaro, Beatriz
de la Cruz, Fernando
Alvarez, Hector Manuel
Moncalián, Gabriel
author_role author
author2 Villa Guerrero, Juan Angel
Lanza, Val F.
Lázaro, Beatriz
de la Cruz, Fernando
Alvarez, Hector Manuel
Moncalián, Gabriel
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv CRP
ENTNER-DOUDOROFF PATHWAY
NUTRIENT STARVATION
RHODOCOCCUS
RNA-SEQ
TRIACYLGLYCEROL
topic CRP
ENTNER-DOUDOROFF PATHWAY
NUTRIENT STARVATION
RHODOCOCCUS
RNA-SEQ
TRIACYLGLYCEROL
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background: Rhodococcus jostii RHA1 and other actinobacteria accumulate triglycerides (TAG) under nutrient starvation. This property has an important biotechnological potential in the production of sustainable oils. Results: To gain insight into the metabolic pathways involved in TAG accumulation, we analysed the transcriptome of R jostii RHA1 under nutrient-limiting conditions. We correlate these physiological conditions with significant changes in cell physiology. The main consequence was a global switch from catabolic to anabolic pathways. Interestingly, the Entner-Doudoroff (ED) pathway was upregulated in detriment of the glycolysis or pentose phosphate pathways. ED induction was independent of the carbon source (either gluconate or glucose). Some of the diacylglycerol acyltransferase genes involved in the last step of the Kennedy pathway were also upregulated. A common feature of the promoter region of most upregulated genes was the presence of a consensus binding sequence for the cAMP-dependent CRP regulator. Conclusion: This is the first experimental observation of an ED shift under nutrient starvation conditions. Knowledge of this switch could help in the design of metabolomic approaches to optimize carbon derivation for single cell oil production.
Fil: Juarez, Antonio. Institut de Bioenginyeria de Catalunya; España
Fil: Villa Guerrero, Juan Angel. Universidad de Cantabria; España
Fil: Lanza, Val F.. Universidad de Cantabria; España
Fil: Lázaro, Beatriz. Universidad de Cantabria; España
Fil: de la Cruz, Fernando. Universidad de Cantabria; España
Fil: Alvarez, Hector Manuel. Universidad Nacional de la Patagonia ; Argentina
Fil: Moncalián, Gabriel. Universidad de Cantabria; España
description Background: Rhodococcus jostii RHA1 and other actinobacteria accumulate triglycerides (TAG) under nutrient starvation. This property has an important biotechnological potential in the production of sustainable oils. Results: To gain insight into the metabolic pathways involved in TAG accumulation, we analysed the transcriptome of R jostii RHA1 under nutrient-limiting conditions. We correlate these physiological conditions with significant changes in cell physiology. The main consequence was a global switch from catabolic to anabolic pathways. Interestingly, the Entner-Doudoroff (ED) pathway was upregulated in detriment of the glycolysis or pentose phosphate pathways. ED induction was independent of the carbon source (either gluconate or glucose). Some of the diacylglycerol acyltransferase genes involved in the last step of the Kennedy pathway were also upregulated. A common feature of the promoter region of most upregulated genes was the presence of a consensus binding sequence for the cAMP-dependent CRP regulator. Conclusion: This is the first experimental observation of an ED shift under nutrient starvation conditions. Knowledge of this switch could help in the design of metabolomic approaches to optimize carbon derivation for single cell oil production.
publishDate 2017
dc.date.none.fl_str_mv 2017-02-27
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/36941
Juarez, Antonio; Villa Guerrero, Juan Angel; Lanza, Val F.; Lázaro, Beatriz; de la Cruz, Fernando; et al.; Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1; BioMed Central; Microbial Cell Factories; 16; 35; 27-2-2017; 1-12
1475-2859
CONICET Digital
CONICET
url http://hdl.handle.net/11336/36941
identifier_str_mv Juarez, Antonio; Villa Guerrero, Juan Angel; Lanza, Val F.; Lázaro, Beatriz; de la Cruz, Fernando; et al.; Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1; BioMed Central; Microbial Cell Factories; 16; 35; 27-2-2017; 1-12
1475-2859
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1186/s12934-017-0651-7
info:eu-repo/semantics/altIdentifier/url/https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-017-0651-7
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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