Identification of a novel ATP-binding cassette transporter involved in long-chain fatty acid import and its role in triacylglycerol accumulation in Rhodococcus jostii RHA1
- Autores
- Villalba, María S.; Alvarez, Hector Manuel
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Members of Rhodococcus genus are specialists in the biosynthesis and accumulation of triacylglycerols (TAG). Since no transport protein related with TAG metabolism has been characterized until now in these bacteria, we used the available genomic information of Rhodococcus jostii RHA1 to perform a broad survey of genes coding for putative lipid transporter proteins in this oleaginous microorganism. Among the seven genes encoding for putative lipid transporters, ro05645 (now called ltp1: lipid transporter protein) coding for an ABC protein was found clustered with others genes encoding for enzymes catalyzing the three putative acylation reactions of the Kennedy pathway for TAG synthesis. Overexpression of ltp1 gene in the RHA1 strain led to an increase of approximately six- and three-folds in biomass and TAG production; respectively, when cells were cultivated on palmitic acid and oleic acid. Moreover, overexpression of ltp1 also promoted a significant increase in the uptake of a fluorescently labeled long-chain fatty acid in comparison with the wild type strain RHA1, and its further incorporation into the TAG fraction. Gluconate-grown cells showed increasing amounts of intracellular free fatty acids, but not of TAG, after overexpressing ltp1 gene. Thus, for the first time to our knowledge, a transporter functionally related to TAG metabolism was identified in oleaginous rhodococci. Our results suggest that Ltp1 is an importer of long chain fatty acids, which plays a functional role in lipid homeostasis of R. jostii RHA1.
Fil: Villalba, María S.. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Comodoro; Argentina
Fil: Alvarez, Hector Manuel. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Comodoro; Argentina. Oil m&s; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina - Materia
-
Rhodococcus
Triacylglycerols
Transporter Proteins - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/3551
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Identification of a novel ATP-binding cassette transporter involved in long-chain fatty acid import and its role in triacylglycerol accumulation in Rhodococcus jostii RHA1Villalba, María S.Alvarez, Hector ManuelRhodococcusTriacylglycerolsTransporter Proteinshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Members of Rhodococcus genus are specialists in the biosynthesis and accumulation of triacylglycerols (TAG). Since no transport protein related with TAG metabolism has been characterized until now in these bacteria, we used the available genomic information of Rhodococcus jostii RHA1 to perform a broad survey of genes coding for putative lipid transporter proteins in this oleaginous microorganism. Among the seven genes encoding for putative lipid transporters, ro05645 (now called ltp1: lipid transporter protein) coding for an ABC protein was found clustered with others genes encoding for enzymes catalyzing the three putative acylation reactions of the Kennedy pathway for TAG synthesis. Overexpression of ltp1 gene in the RHA1 strain led to an increase of approximately six- and three-folds in biomass and TAG production; respectively, when cells were cultivated on palmitic acid and oleic acid. Moreover, overexpression of ltp1 also promoted a significant increase in the uptake of a fluorescently labeled long-chain fatty acid in comparison with the wild type strain RHA1, and its further incorporation into the TAG fraction. Gluconate-grown cells showed increasing amounts of intracellular free fatty acids, but not of TAG, after overexpressing ltp1 gene. Thus, for the first time to our knowledge, a transporter functionally related to TAG metabolism was identified in oleaginous rhodococci. Our results suggest that Ltp1 is an importer of long chain fatty acids, which plays a functional role in lipid homeostasis of R. jostii RHA1.Fil: Villalba, María S.. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Comodoro; ArgentinaFil: Alvarez, Hector Manuel. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Comodoro; Argentina. Oil m&s; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; ArgentinaMicrobiology Society2014-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/3551Villalba, María S.; Alvarez, Hector Manuel; Identification of a novel ATP-binding cassette transporter involved in long-chain fatty acid import and its role in triacylglycerol accumulation in Rhodococcus jostii RHA1; Microbiology Society; Microbiology-uk; 160; 1-7-2014; 1523-15321350-0872enginfo:eu-repo/semantics/altIdentifier/url/http://mic.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.078477-0info:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1099/mic.0.078477-0info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:56:05Zoai:ri.conicet.gov.ar:11336/3551instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:56:06.104CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Identification of a novel ATP-binding cassette transporter involved in long-chain fatty acid import and its role in triacylglycerol accumulation in Rhodococcus jostii RHA1 |
title |
Identification of a novel ATP-binding cassette transporter involved in long-chain fatty acid import and its role in triacylglycerol accumulation in Rhodococcus jostii RHA1 |
spellingShingle |
Identification of a novel ATP-binding cassette transporter involved in long-chain fatty acid import and its role in triacylglycerol accumulation in Rhodococcus jostii RHA1 Villalba, María S. Rhodococcus Triacylglycerols Transporter Proteins |
title_short |
Identification of a novel ATP-binding cassette transporter involved in long-chain fatty acid import and its role in triacylglycerol accumulation in Rhodococcus jostii RHA1 |
title_full |
Identification of a novel ATP-binding cassette transporter involved in long-chain fatty acid import and its role in triacylglycerol accumulation in Rhodococcus jostii RHA1 |
title_fullStr |
Identification of a novel ATP-binding cassette transporter involved in long-chain fatty acid import and its role in triacylglycerol accumulation in Rhodococcus jostii RHA1 |
title_full_unstemmed |
Identification of a novel ATP-binding cassette transporter involved in long-chain fatty acid import and its role in triacylglycerol accumulation in Rhodococcus jostii RHA1 |
title_sort |
Identification of a novel ATP-binding cassette transporter involved in long-chain fatty acid import and its role in triacylglycerol accumulation in Rhodococcus jostii RHA1 |
dc.creator.none.fl_str_mv |
Villalba, María S. Alvarez, Hector Manuel |
author |
Villalba, María S. |
author_facet |
Villalba, María S. Alvarez, Hector Manuel |
author_role |
author |
author2 |
Alvarez, Hector Manuel |
author2_role |
author |
dc.subject.none.fl_str_mv |
Rhodococcus Triacylglycerols Transporter Proteins |
topic |
Rhodococcus Triacylglycerols Transporter Proteins |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Members of Rhodococcus genus are specialists in the biosynthesis and accumulation of triacylglycerols (TAG). Since no transport protein related with TAG metabolism has been characterized until now in these bacteria, we used the available genomic information of Rhodococcus jostii RHA1 to perform a broad survey of genes coding for putative lipid transporter proteins in this oleaginous microorganism. Among the seven genes encoding for putative lipid transporters, ro05645 (now called ltp1: lipid transporter protein) coding for an ABC protein was found clustered with others genes encoding for enzymes catalyzing the three putative acylation reactions of the Kennedy pathway for TAG synthesis. Overexpression of ltp1 gene in the RHA1 strain led to an increase of approximately six- and three-folds in biomass and TAG production; respectively, when cells were cultivated on palmitic acid and oleic acid. Moreover, overexpression of ltp1 also promoted a significant increase in the uptake of a fluorescently labeled long-chain fatty acid in comparison with the wild type strain RHA1, and its further incorporation into the TAG fraction. Gluconate-grown cells showed increasing amounts of intracellular free fatty acids, but not of TAG, after overexpressing ltp1 gene. Thus, for the first time to our knowledge, a transporter functionally related to TAG metabolism was identified in oleaginous rhodococci. Our results suggest that Ltp1 is an importer of long chain fatty acids, which plays a functional role in lipid homeostasis of R. jostii RHA1. Fil: Villalba, María S.. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Comodoro; Argentina Fil: Alvarez, Hector Manuel. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Comodoro; Argentina. Oil m&s; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina |
description |
Members of Rhodococcus genus are specialists in the biosynthesis and accumulation of triacylglycerols (TAG). Since no transport protein related with TAG metabolism has been characterized until now in these bacteria, we used the available genomic information of Rhodococcus jostii RHA1 to perform a broad survey of genes coding for putative lipid transporter proteins in this oleaginous microorganism. Among the seven genes encoding for putative lipid transporters, ro05645 (now called ltp1: lipid transporter protein) coding for an ABC protein was found clustered with others genes encoding for enzymes catalyzing the three putative acylation reactions of the Kennedy pathway for TAG synthesis. Overexpression of ltp1 gene in the RHA1 strain led to an increase of approximately six- and three-folds in biomass and TAG production; respectively, when cells were cultivated on palmitic acid and oleic acid. Moreover, overexpression of ltp1 also promoted a significant increase in the uptake of a fluorescently labeled long-chain fatty acid in comparison with the wild type strain RHA1, and its further incorporation into the TAG fraction. Gluconate-grown cells showed increasing amounts of intracellular free fatty acids, but not of TAG, after overexpressing ltp1 gene. Thus, for the first time to our knowledge, a transporter functionally related to TAG metabolism was identified in oleaginous rhodococci. Our results suggest that Ltp1 is an importer of long chain fatty acids, which plays a functional role in lipid homeostasis of R. jostii RHA1. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-07-01 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/3551 Villalba, María S.; Alvarez, Hector Manuel; Identification of a novel ATP-binding cassette transporter involved in long-chain fatty acid import and its role in triacylglycerol accumulation in Rhodococcus jostii RHA1; Microbiology Society; Microbiology-uk; 160; 1-7-2014; 1523-1532 1350-0872 |
url |
http://hdl.handle.net/11336/3551 |
identifier_str_mv |
Villalba, María S.; Alvarez, Hector Manuel; Identification of a novel ATP-binding cassette transporter involved in long-chain fatty acid import and its role in triacylglycerol accumulation in Rhodococcus jostii RHA1; Microbiology Society; Microbiology-uk; 160; 1-7-2014; 1523-1532 1350-0872 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://mic.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.078477-0 info:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1099/mic.0.078477-0 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Microbiology Society |
publisher.none.fl_str_mv |
Microbiology Society |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842269383908392960 |
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13.13397 |