Identification of a novel ATP-binding cassette transporter involved in long-chain fatty acid import and its role in triacylglycerol accumulation in Rhodococcus jostii RHA1

Autores
Villalba, María S.; Alvarez, Hector Manuel
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Members of Rhodococcus genus are specialists in the biosynthesis and accumulation of triacylglycerols (TAG). Since no transport protein related with TAG metabolism has been characterized until now in these bacteria, we used the available genomic information of Rhodococcus jostii RHA1 to perform a broad survey of genes coding for putative lipid transporter proteins in this oleaginous microorganism. Among the seven genes encoding for putative lipid transporters, ro05645 (now called ltp1: lipid transporter protein) coding for an ABC protein was found clustered with others genes encoding for enzymes catalyzing the three putative acylation reactions of the Kennedy pathway for TAG synthesis. Overexpression of ltp1 gene in the RHA1 strain led to an increase of approximately six- and three-folds in biomass and TAG production; respectively, when cells were cultivated on palmitic acid and oleic acid. Moreover, overexpression of ltp1 also promoted a significant increase in the uptake of a fluorescently labeled long-chain fatty acid in comparison with the wild type strain RHA1, and its further incorporation into the TAG fraction. Gluconate-grown cells showed increasing amounts of intracellular free fatty acids, but not of TAG, after overexpressing ltp1 gene. Thus, for the first time to our knowledge, a transporter functionally related to TAG metabolism was identified in oleaginous rhodococci. Our results suggest that Ltp1 is an importer of long chain fatty acids, which plays a functional role in lipid homeostasis of R. jostii RHA1.
Fil: Villalba, María S.. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Comodoro; Argentina
Fil: Alvarez, Hector Manuel. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Comodoro; Argentina. Oil m&s; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
Materia
Rhodococcus
Triacylglycerols
Transporter Proteins
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/3551
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Identification of a novel ATP-binding cassette transporter involved in long-chain fatty acid import and its role in triacylglycerol accumulation in Rhodococcus jostii RHA1Villalba, María S.Alvarez, Hector ManuelRhodococcusTriacylglycerolsTransporter Proteinshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Members of Rhodococcus genus are specialists in the biosynthesis and accumulation of triacylglycerols (TAG). Since no transport protein related with TAG metabolism has been characterized until now in these bacteria, we used the available genomic information of Rhodococcus jostii RHA1 to perform a broad survey of genes coding for putative lipid transporter proteins in this oleaginous microorganism. Among the seven genes encoding for putative lipid transporters, ro05645 (now called ltp1: lipid transporter protein) coding for an ABC protein was found clustered with others genes encoding for enzymes catalyzing the three putative acylation reactions of the Kennedy pathway for TAG synthesis. Overexpression of ltp1 gene in the RHA1 strain led to an increase of approximately six- and three-folds in biomass and TAG production; respectively, when cells were cultivated on palmitic acid and oleic acid. Moreover, overexpression of ltp1 also promoted a significant increase in the uptake of a fluorescently labeled long-chain fatty acid in comparison with the wild type strain RHA1, and its further incorporation into the TAG fraction. Gluconate-grown cells showed increasing amounts of intracellular free fatty acids, but not of TAG, after overexpressing ltp1 gene. Thus, for the first time to our knowledge, a transporter functionally related to TAG metabolism was identified in oleaginous rhodococci. Our results suggest that Ltp1 is an importer of long chain fatty acids, which plays a functional role in lipid homeostasis of R. jostii RHA1.Fil: Villalba, María S.. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Comodoro; ArgentinaFil: Alvarez, Hector Manuel. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Comodoro; Argentina. Oil m&s; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; ArgentinaMicrobiology Society2014-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/3551Villalba, María S.; Alvarez, Hector Manuel; Identification of a novel ATP-binding cassette transporter involved in long-chain fatty acid import and its role in triacylglycerol accumulation in Rhodococcus jostii RHA1; Microbiology Society; Microbiology-uk; 160; 1-7-2014; 1523-15321350-0872enginfo:eu-repo/semantics/altIdentifier/url/http://mic.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.078477-0info:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1099/mic.0.078477-0info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:59:46Zoai:ri.conicet.gov.ar:11336/3551instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:59:46.843CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Identification of a novel ATP-binding cassette transporter involved in long-chain fatty acid import and its role in triacylglycerol accumulation in Rhodococcus jostii RHA1
title Identification of a novel ATP-binding cassette transporter involved in long-chain fatty acid import and its role in triacylglycerol accumulation in Rhodococcus jostii RHA1
spellingShingle Identification of a novel ATP-binding cassette transporter involved in long-chain fatty acid import and its role in triacylglycerol accumulation in Rhodococcus jostii RHA1
Villalba, María S.
Rhodococcus
Triacylglycerols
Transporter Proteins
title_short Identification of a novel ATP-binding cassette transporter involved in long-chain fatty acid import and its role in triacylglycerol accumulation in Rhodococcus jostii RHA1
title_full Identification of a novel ATP-binding cassette transporter involved in long-chain fatty acid import and its role in triacylglycerol accumulation in Rhodococcus jostii RHA1
title_fullStr Identification of a novel ATP-binding cassette transporter involved in long-chain fatty acid import and its role in triacylglycerol accumulation in Rhodococcus jostii RHA1
title_full_unstemmed Identification of a novel ATP-binding cassette transporter involved in long-chain fatty acid import and its role in triacylglycerol accumulation in Rhodococcus jostii RHA1
title_sort Identification of a novel ATP-binding cassette transporter involved in long-chain fatty acid import and its role in triacylglycerol accumulation in Rhodococcus jostii RHA1
dc.creator.none.fl_str_mv Villalba, María S.
Alvarez, Hector Manuel
author Villalba, María S.
author_facet Villalba, María S.
Alvarez, Hector Manuel
author_role author
author2 Alvarez, Hector Manuel
author2_role author
dc.subject.none.fl_str_mv Rhodococcus
Triacylglycerols
Transporter Proteins
topic Rhodococcus
Triacylglycerols
Transporter Proteins
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Members of Rhodococcus genus are specialists in the biosynthesis and accumulation of triacylglycerols (TAG). Since no transport protein related with TAG metabolism has been characterized until now in these bacteria, we used the available genomic information of Rhodococcus jostii RHA1 to perform a broad survey of genes coding for putative lipid transporter proteins in this oleaginous microorganism. Among the seven genes encoding for putative lipid transporters, ro05645 (now called ltp1: lipid transporter protein) coding for an ABC protein was found clustered with others genes encoding for enzymes catalyzing the three putative acylation reactions of the Kennedy pathway for TAG synthesis. Overexpression of ltp1 gene in the RHA1 strain led to an increase of approximately six- and three-folds in biomass and TAG production; respectively, when cells were cultivated on palmitic acid and oleic acid. Moreover, overexpression of ltp1 also promoted a significant increase in the uptake of a fluorescently labeled long-chain fatty acid in comparison with the wild type strain RHA1, and its further incorporation into the TAG fraction. Gluconate-grown cells showed increasing amounts of intracellular free fatty acids, but not of TAG, after overexpressing ltp1 gene. Thus, for the first time to our knowledge, a transporter functionally related to TAG metabolism was identified in oleaginous rhodococci. Our results suggest that Ltp1 is an importer of long chain fatty acids, which plays a functional role in lipid homeostasis of R. jostii RHA1.
Fil: Villalba, María S.. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Comodoro; Argentina
Fil: Alvarez, Hector Manuel. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Comodoro; Argentina. Oil m&s; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
description Members of Rhodococcus genus are specialists in the biosynthesis and accumulation of triacylglycerols (TAG). Since no transport protein related with TAG metabolism has been characterized until now in these bacteria, we used the available genomic information of Rhodococcus jostii RHA1 to perform a broad survey of genes coding for putative lipid transporter proteins in this oleaginous microorganism. Among the seven genes encoding for putative lipid transporters, ro05645 (now called ltp1: lipid transporter protein) coding for an ABC protein was found clustered with others genes encoding for enzymes catalyzing the three putative acylation reactions of the Kennedy pathway for TAG synthesis. Overexpression of ltp1 gene in the RHA1 strain led to an increase of approximately six- and three-folds in biomass and TAG production; respectively, when cells were cultivated on palmitic acid and oleic acid. Moreover, overexpression of ltp1 also promoted a significant increase in the uptake of a fluorescently labeled long-chain fatty acid in comparison with the wild type strain RHA1, and its further incorporation into the TAG fraction. Gluconate-grown cells showed increasing amounts of intracellular free fatty acids, but not of TAG, after overexpressing ltp1 gene. Thus, for the first time to our knowledge, a transporter functionally related to TAG metabolism was identified in oleaginous rhodococci. Our results suggest that Ltp1 is an importer of long chain fatty acids, which plays a functional role in lipid homeostasis of R. jostii RHA1.
publishDate 2014
dc.date.none.fl_str_mv 2014-07-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/3551
Villalba, María S.; Alvarez, Hector Manuel; Identification of a novel ATP-binding cassette transporter involved in long-chain fatty acid import and its role in triacylglycerol accumulation in Rhodococcus jostii RHA1; Microbiology Society; Microbiology-uk; 160; 1-7-2014; 1523-1532
1350-0872
url http://hdl.handle.net/11336/3551
identifier_str_mv Villalba, María S.; Alvarez, Hector Manuel; Identification of a novel ATP-binding cassette transporter involved in long-chain fatty acid import and its role in triacylglycerol accumulation in Rhodococcus jostii RHA1; Microbiology Society; Microbiology-uk; 160; 1-7-2014; 1523-1532
1350-0872
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://mic.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.078477-0
info:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1099/mic.0.078477-0
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Microbiology Society
publisher.none.fl_str_mv Microbiology Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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