The Entner–Doudoroff pathway empowers Pseudomonas putida KT2440 with a high tolerance to oxidative stress

Autores
Chavarría, Max; Nikel, Pablo Ivan; Perez Pantoja, Danilo; de Lorenzo, Víctor
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Glucose catabolism of Pseudomonas putida is carried out exclusively through the Entner–Doudoroff (ED) pathway due to the absence of 6-phosphofructokinase. In order to activate the Embden–Meyerhof– Parnas (EMP) route we transferred the pfkA gene from Escherichia coli to a P. putida wild-type strain as well as to an eda mutant, i.e. lacking 2-keto-3-deoxy-6- phosphogluconate aldolase. PfkAE. coli failed to redirect the carbon flow from the ED route towards the EMP pathway, suggesting that ED was essential for sugar catabolism. The presence of PfkAE. coli was detrimental for growth, which could be traced to the reduction of ATP and NAD(P)H pools along with alteration of the NAD(P)H/NADP+ ratio. Pseudomonas putida cells carrying PfkAE. coli became highly sensitive to diamide and hydrogen peroxide, the response to which is very demanding of NADPH. The inhibitory effect of PfkAE. coli could in part be relieved by methionine, the synthesis of which relies much on NADPH. These results expose the role of the ED pathway for generating the redox currency (NADPH) that is required for counteracting oxidative stress. It is thus likely that environmental bacteria that favour the ED pathway over the EMP pathway do so in order to gear their aerobic metabolism to endure oxidative-related insults.
Fil: Chavarría, Max. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; España. Universidad de Costa Rica; Costa Rica
Fil: Nikel, Pablo Ivan. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Perez Pantoja, Danilo. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; España
Fil: de Lorenzo, Víctor. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; España
Materia
Pseudomonas Putida Kt2440
Pfk
Escherichia Coli
Entner-Doudoroff
Metabolic Engineering
Redox Homeostasis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/24508
Acceder
id CONICETDig_b28fa4dfdf229c396210a7d7092c397e
oai_identifier_str oai:ri.conicet.gov.ar:11336/24508
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The Entner–Doudoroff pathway empowers Pseudomonas putida KT2440 with a high tolerance to oxidative stressChavarría, MaxNikel, Pablo IvanPerez Pantoja, Danilode Lorenzo, VíctorPseudomonas Putida Kt2440PfkEscherichia ColiEntner-DoudoroffMetabolic EngineeringRedox Homeostasishttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Glucose catabolism of Pseudomonas putida is carried out exclusively through the Entner–Doudoroff (ED) pathway due to the absence of 6-phosphofructokinase. In order to activate the Embden–Meyerhof– Parnas (EMP) route we transferred the pfkA gene from Escherichia coli to a P. putida wild-type strain as well as to an eda mutant, i.e. lacking 2-keto-3-deoxy-6- phosphogluconate aldolase. PfkAE. coli failed to redirect the carbon flow from the ED route towards the EMP pathway, suggesting that ED was essential for sugar catabolism. The presence of PfkAE. coli was detrimental for growth, which could be traced to the reduction of ATP and NAD(P)H pools along with alteration of the NAD(P)H/NADP+ ratio. Pseudomonas putida cells carrying PfkAE. coli became highly sensitive to diamide and hydrogen peroxide, the response to which is very demanding of NADPH. The inhibitory effect of PfkAE. coli could in part be relieved by methionine, the synthesis of which relies much on NADPH. These results expose the role of the ED pathway for generating the redox currency (NADPH) that is required for counteracting oxidative stress. It is thus likely that environmental bacteria that favour the ED pathway over the EMP pathway do so in order to gear their aerobic metabolism to endure oxidative-related insults.Fil: Chavarría, Max. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; España. Universidad de Costa Rica; Costa RicaFil: Nikel, Pablo Ivan. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; España. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Perez Pantoja, Danilo. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; EspañaFil: de Lorenzo, Víctor. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; EspañaWiley Blackwell Publishing, Inc2013-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24508Chavarría, Max; Nikel, Pablo Ivan; Perez Pantoja, Danilo; de Lorenzo, Víctor; The Entner–Doudoroff pathway empowers Pseudomonas putida KT2440 with a high tolerance to oxidative stress; Wiley Blackwell Publishing, Inc; Environmental Microbiology; 15; 6; 6-2013; 1772-17851462-2912CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/1462-2920.12069info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/1462-2920.12069/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:57:31Zoai:ri.conicet.gov.ar:11336/24508instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:57:32.138CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The Entner–Doudoroff pathway empowers Pseudomonas putida KT2440 with a high tolerance to oxidative stress
title The Entner–Doudoroff pathway empowers Pseudomonas putida KT2440 with a high tolerance to oxidative stress
spellingShingle The Entner–Doudoroff pathway empowers Pseudomonas putida KT2440 with a high tolerance to oxidative stress
Chavarría, Max
Pseudomonas Putida Kt2440
Pfk
Escherichia Coli
Entner-Doudoroff
Metabolic Engineering
Redox Homeostasis
title_short The Entner–Doudoroff pathway empowers Pseudomonas putida KT2440 with a high tolerance to oxidative stress
title_full The Entner–Doudoroff pathway empowers Pseudomonas putida KT2440 with a high tolerance to oxidative stress
title_fullStr The Entner–Doudoroff pathway empowers Pseudomonas putida KT2440 with a high tolerance to oxidative stress
title_full_unstemmed The Entner–Doudoroff pathway empowers Pseudomonas putida KT2440 with a high tolerance to oxidative stress
title_sort The Entner–Doudoroff pathway empowers Pseudomonas putida KT2440 with a high tolerance to oxidative stress
dc.creator.none.fl_str_mv Chavarría, Max
Nikel, Pablo Ivan
Perez Pantoja, Danilo
de Lorenzo, Víctor
author Chavarría, Max
author_facet Chavarría, Max
Nikel, Pablo Ivan
Perez Pantoja, Danilo
de Lorenzo, Víctor
author_role author
author2 Nikel, Pablo Ivan
Perez Pantoja, Danilo
de Lorenzo, Víctor
author2_role author
author
author
dc.subject.none.fl_str_mv Pseudomonas Putida Kt2440
Pfk
Escherichia Coli
Entner-Doudoroff
Metabolic Engineering
Redox Homeostasis
topic Pseudomonas Putida Kt2440
Pfk
Escherichia Coli
Entner-Doudoroff
Metabolic Engineering
Redox Homeostasis
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Glucose catabolism of Pseudomonas putida is carried out exclusively through the Entner–Doudoroff (ED) pathway due to the absence of 6-phosphofructokinase. In order to activate the Embden–Meyerhof– Parnas (EMP) route we transferred the pfkA gene from Escherichia coli to a P. putida wild-type strain as well as to an eda mutant, i.e. lacking 2-keto-3-deoxy-6- phosphogluconate aldolase. PfkAE. coli failed to redirect the carbon flow from the ED route towards the EMP pathway, suggesting that ED was essential for sugar catabolism. The presence of PfkAE. coli was detrimental for growth, which could be traced to the reduction of ATP and NAD(P)H pools along with alteration of the NAD(P)H/NADP+ ratio. Pseudomonas putida cells carrying PfkAE. coli became highly sensitive to diamide and hydrogen peroxide, the response to which is very demanding of NADPH. The inhibitory effect of PfkAE. coli could in part be relieved by methionine, the synthesis of which relies much on NADPH. These results expose the role of the ED pathway for generating the redox currency (NADPH) that is required for counteracting oxidative stress. It is thus likely that environmental bacteria that favour the ED pathway over the EMP pathway do so in order to gear their aerobic metabolism to endure oxidative-related insults.
Fil: Chavarría, Max. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; España. Universidad de Costa Rica; Costa Rica
Fil: Nikel, Pablo Ivan. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Perez Pantoja, Danilo. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; España
Fil: de Lorenzo, Víctor. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; España
description Glucose catabolism of Pseudomonas putida is carried out exclusively through the Entner–Doudoroff (ED) pathway due to the absence of 6-phosphofructokinase. In order to activate the Embden–Meyerhof– Parnas (EMP) route we transferred the pfkA gene from Escherichia coli to a P. putida wild-type strain as well as to an eda mutant, i.e. lacking 2-keto-3-deoxy-6- phosphogluconate aldolase. PfkAE. coli failed to redirect the carbon flow from the ED route towards the EMP pathway, suggesting that ED was essential for sugar catabolism. The presence of PfkAE. coli was detrimental for growth, which could be traced to the reduction of ATP and NAD(P)H pools along with alteration of the NAD(P)H/NADP+ ratio. Pseudomonas putida cells carrying PfkAE. coli became highly sensitive to diamide and hydrogen peroxide, the response to which is very demanding of NADPH. The inhibitory effect of PfkAE. coli could in part be relieved by methionine, the synthesis of which relies much on NADPH. These results expose the role of the ED pathway for generating the redox currency (NADPH) that is required for counteracting oxidative stress. It is thus likely that environmental bacteria that favour the ED pathway over the EMP pathway do so in order to gear their aerobic metabolism to endure oxidative-related insults.
publishDate 2013
dc.date.none.fl_str_mv 2013-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/24508
Chavarría, Max; Nikel, Pablo Ivan; Perez Pantoja, Danilo; de Lorenzo, Víctor; The Entner–Doudoroff pathway empowers Pseudomonas putida KT2440 with a high tolerance to oxidative stress; Wiley Blackwell Publishing, Inc; Environmental Microbiology; 15; 6; 6-2013; 1772-1785
1462-2912
CONICET Digital
CONICET
url http://hdl.handle.net/11336/24508
identifier_str_mv Chavarría, Max; Nikel, Pablo Ivan; Perez Pantoja, Danilo; de Lorenzo, Víctor; The Entner–Doudoroff pathway empowers Pseudomonas putida KT2440 with a high tolerance to oxidative stress; Wiley Blackwell Publishing, Inc; Environmental Microbiology; 15; 6; 6-2013; 1772-1785
1462-2912
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/1462-2920.12069
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/1462-2920.12069/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269467146452992
score 13.13397

Publicaciones similares