Carbon allocation in Rhodococcus jostii RHA1 in response to disruption and overexpression of nlpR Regulatory Gene, based on 13C-labeling analysis

Autores
Hernández, Martín Alejandro; Gleixner, Gerd; Sachse, Dirk; Alvarez, Hector Manuel
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Nitrogen lipid regulator (NlpR) is a pleiotropic regulator that positively controls genes associated with both nitrogen and lipid metabolism in the oleaginous bacterium Rhodococcus jostii RHA1. In this study, we investigated the effect of nlpR disruption and overexpression on the assimilation of 13C-labeled glucose as carbon source, during cultivation of cells under nitrogen-limiting and nitrogen-rich conditions, respectively. Label incorporation into the total lipid extract (TLE) fraction was about 30% lower in the mutant strain in comparison with the wild type strain under low-nitrogen conditions. Moreover, a higher 13C abundance (~60%) into the extracellular polymeric substance fraction was observed in the mutant strain. nlpR disruption also promoted a decrease in the label incorporation into several TLE-derivative fractions including neutral lipids (NL), glycolipids (GL), phospholipids (PL), triacylglycerols (TAG), diacylglycerols (DAG), and free fatty acids (FFA), with the DAG being the most affected. In contrast, the nlpR overexpression in RHA1 cells under nitrogen-rich conditions produced an increase of the label incorporation into the TLE and its derivative NL and PL fractions, the last one being the highest 13C enriched. In addition, a higher 13C enrichment occurred in the TAG, DAG, and FFA fractions after nlpR induction, with the FFA fraction being the most affected within the TLE. Isotopic-labeling experiments demonstrated that NlpR regulator is contributing in oleaginous phenotype of R. jostii RHA1 to the allocation of carbon into the different lipid fractions in response to nitrogen levels, increasing the rate of carbon flux into lipid metabolism.
Fil: Hernández, Martín Alejandro. Universidad Nacional de la Patagonia "san Juan Bosco". Instituto de Biociencias de la Patagonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Biociencias de la Patagonia; Argentina
Fil: Gleixner, Gerd. Max Planck Institute for Biogeochemistry; Alemania
Fil: Sachse, Dirk. Universitat Potsdam; Alemania. German Research Centre for Geosciences; Alemania
Fil: Alvarez, Hector Manuel. Universidad Nacional de la Patagonia "san Juan Bosco". Instituto de Biociencias de la Patagonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Biociencias de la Patagonia; Argentina
Materia
13C-GLUCOSE
LIPID METABOLISM
NLPR
REGULATION
RHA1
RHODOCOCCUS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/37032
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/37032
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Carbon allocation in Rhodococcus jostii RHA1 in response to disruption and overexpression of nlpR Regulatory Gene, based on 13C-labeling analysisHernández, Martín AlejandroGleixner, GerdSachse, DirkAlvarez, Hector Manuel13C-GLUCOSELIPID METABOLISMNLPRREGULATIONRHA1RHODOCOCCUShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Nitrogen lipid regulator (NlpR) is a pleiotropic regulator that positively controls genes associated with both nitrogen and lipid metabolism in the oleaginous bacterium Rhodococcus jostii RHA1. In this study, we investigated the effect of nlpR disruption and overexpression on the assimilation of 13C-labeled glucose as carbon source, during cultivation of cells under nitrogen-limiting and nitrogen-rich conditions, respectively. Label incorporation into the total lipid extract (TLE) fraction was about 30% lower in the mutant strain in comparison with the wild type strain under low-nitrogen conditions. Moreover, a higher 13C abundance (~60%) into the extracellular polymeric substance fraction was observed in the mutant strain. nlpR disruption also promoted a decrease in the label incorporation into several TLE-derivative fractions including neutral lipids (NL), glycolipids (GL), phospholipids (PL), triacylglycerols (TAG), diacylglycerols (DAG), and free fatty acids (FFA), with the DAG being the most affected. In contrast, the nlpR overexpression in RHA1 cells under nitrogen-rich conditions produced an increase of the label incorporation into the TLE and its derivative NL and PL fractions, the last one being the highest 13C enriched. In addition, a higher 13C enrichment occurred in the TAG, DAG, and FFA fractions after nlpR induction, with the FFA fraction being the most affected within the TLE. Isotopic-labeling experiments demonstrated that NlpR regulator is contributing in oleaginous phenotype of R. jostii RHA1 to the allocation of carbon into the different lipid fractions in response to nitrogen levels, increasing the rate of carbon flux into lipid metabolism.Fil: Hernández, Martín Alejandro. Universidad Nacional de la Patagonia "san Juan Bosco". Instituto de Biociencias de la Patagonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Biociencias de la Patagonia; ArgentinaFil: Gleixner, Gerd. Max Planck Institute for Biogeochemistry; AlemaniaFil: Sachse, Dirk. Universitat Potsdam; Alemania. German Research Centre for Geosciences; AlemaniaFil: Alvarez, Hector Manuel. Universidad Nacional de la Patagonia "san Juan Bosco". Instituto de Biociencias de la Patagonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Biociencias de la Patagonia; ArgentinaFrontiers Media S.A.2017-10-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/37032Hernández, Martín Alejandro; Gleixner, Gerd; Sachse, Dirk; Alvarez, Hector Manuel; Carbon allocation in Rhodococcus jostii RHA1 in response to disruption and overexpression of nlpR Regulatory Gene, based on 13C-labeling analysis; Frontiers Media S.A.; Frontiers in Microbiology; 8; 1992; 11-10-2017; 1-111664-302XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2017.01992info:eu-repo/semantics/altIdentifier/url/journal.frontiersin.org/Journal/10.3389/fmicb.2017.01992/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:10:05Zoai:ri.conicet.gov.ar:11336/37032instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:10:06.056CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Carbon allocation in Rhodococcus jostii RHA1 in response to disruption and overexpression of nlpR Regulatory Gene, based on 13C-labeling analysis
title Carbon allocation in Rhodococcus jostii RHA1 in response to disruption and overexpression of nlpR Regulatory Gene, based on 13C-labeling analysis
spellingShingle Carbon allocation in Rhodococcus jostii RHA1 in response to disruption and overexpression of nlpR Regulatory Gene, based on 13C-labeling analysis
Hernández, Martín Alejandro
13C-GLUCOSE
LIPID METABOLISM
NLPR
REGULATION
RHA1
RHODOCOCCUS
title_short Carbon allocation in Rhodococcus jostii RHA1 in response to disruption and overexpression of nlpR Regulatory Gene, based on 13C-labeling analysis
title_full Carbon allocation in Rhodococcus jostii RHA1 in response to disruption and overexpression of nlpR Regulatory Gene, based on 13C-labeling analysis
title_fullStr Carbon allocation in Rhodococcus jostii RHA1 in response to disruption and overexpression of nlpR Regulatory Gene, based on 13C-labeling analysis
title_full_unstemmed Carbon allocation in Rhodococcus jostii RHA1 in response to disruption and overexpression of nlpR Regulatory Gene, based on 13C-labeling analysis
title_sort Carbon allocation in Rhodococcus jostii RHA1 in response to disruption and overexpression of nlpR Regulatory Gene, based on 13C-labeling analysis
dc.creator.none.fl_str_mv Hernández, Martín Alejandro
Gleixner, Gerd
Sachse, Dirk
Alvarez, Hector Manuel
author Hernández, Martín Alejandro
author_facet Hernández, Martín Alejandro
Gleixner, Gerd
Sachse, Dirk
Alvarez, Hector Manuel
author_role author
author2 Gleixner, Gerd
Sachse, Dirk
Alvarez, Hector Manuel
author2_role author
author
author
dc.subject.none.fl_str_mv 13C-GLUCOSE
LIPID METABOLISM
NLPR
REGULATION
RHA1
RHODOCOCCUS
topic 13C-GLUCOSE
LIPID METABOLISM
NLPR
REGULATION
RHA1
RHODOCOCCUS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Nitrogen lipid regulator (NlpR) is a pleiotropic regulator that positively controls genes associated with both nitrogen and lipid metabolism in the oleaginous bacterium Rhodococcus jostii RHA1. In this study, we investigated the effect of nlpR disruption and overexpression on the assimilation of 13C-labeled glucose as carbon source, during cultivation of cells under nitrogen-limiting and nitrogen-rich conditions, respectively. Label incorporation into the total lipid extract (TLE) fraction was about 30% lower in the mutant strain in comparison with the wild type strain under low-nitrogen conditions. Moreover, a higher 13C abundance (~60%) into the extracellular polymeric substance fraction was observed in the mutant strain. nlpR disruption also promoted a decrease in the label incorporation into several TLE-derivative fractions including neutral lipids (NL), glycolipids (GL), phospholipids (PL), triacylglycerols (TAG), diacylglycerols (DAG), and free fatty acids (FFA), with the DAG being the most affected. In contrast, the nlpR overexpression in RHA1 cells under nitrogen-rich conditions produced an increase of the label incorporation into the TLE and its derivative NL and PL fractions, the last one being the highest 13C enriched. In addition, a higher 13C enrichment occurred in the TAG, DAG, and FFA fractions after nlpR induction, with the FFA fraction being the most affected within the TLE. Isotopic-labeling experiments demonstrated that NlpR regulator is contributing in oleaginous phenotype of R. jostii RHA1 to the allocation of carbon into the different lipid fractions in response to nitrogen levels, increasing the rate of carbon flux into lipid metabolism.
Fil: Hernández, Martín Alejandro. Universidad Nacional de la Patagonia "san Juan Bosco". Instituto de Biociencias de la Patagonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Biociencias de la Patagonia; Argentina
Fil: Gleixner, Gerd. Max Planck Institute for Biogeochemistry; Alemania
Fil: Sachse, Dirk. Universitat Potsdam; Alemania. German Research Centre for Geosciences; Alemania
Fil: Alvarez, Hector Manuel. Universidad Nacional de la Patagonia "san Juan Bosco". Instituto de Biociencias de la Patagonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Biociencias de la Patagonia; Argentina
description Nitrogen lipid regulator (NlpR) is a pleiotropic regulator that positively controls genes associated with both nitrogen and lipid metabolism in the oleaginous bacterium Rhodococcus jostii RHA1. In this study, we investigated the effect of nlpR disruption and overexpression on the assimilation of 13C-labeled glucose as carbon source, during cultivation of cells under nitrogen-limiting and nitrogen-rich conditions, respectively. Label incorporation into the total lipid extract (TLE) fraction was about 30% lower in the mutant strain in comparison with the wild type strain under low-nitrogen conditions. Moreover, a higher 13C abundance (~60%) into the extracellular polymeric substance fraction was observed in the mutant strain. nlpR disruption also promoted a decrease in the label incorporation into several TLE-derivative fractions including neutral lipids (NL), glycolipids (GL), phospholipids (PL), triacylglycerols (TAG), diacylglycerols (DAG), and free fatty acids (FFA), with the DAG being the most affected. In contrast, the nlpR overexpression in RHA1 cells under nitrogen-rich conditions produced an increase of the label incorporation into the TLE and its derivative NL and PL fractions, the last one being the highest 13C enriched. In addition, a higher 13C enrichment occurred in the TAG, DAG, and FFA fractions after nlpR induction, with the FFA fraction being the most affected within the TLE. Isotopic-labeling experiments demonstrated that NlpR regulator is contributing in oleaginous phenotype of R. jostii RHA1 to the allocation of carbon into the different lipid fractions in response to nitrogen levels, increasing the rate of carbon flux into lipid metabolism.
publishDate 2017
dc.date.none.fl_str_mv 2017-10-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/37032
Hernández, Martín Alejandro; Gleixner, Gerd; Sachse, Dirk; Alvarez, Hector Manuel; Carbon allocation in Rhodococcus jostii RHA1 in response to disruption and overexpression of nlpR Regulatory Gene, based on 13C-labeling analysis; Frontiers Media S.A.; Frontiers in Microbiology; 8; 1992; 11-10-2017; 1-11
1664-302X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/37032
identifier_str_mv Hernández, Martín Alejandro; Gleixner, Gerd; Sachse, Dirk; Alvarez, Hector Manuel; Carbon allocation in Rhodococcus jostii RHA1 in response to disruption and overexpression of nlpR Regulatory Gene, based on 13C-labeling analysis; Frontiers Media S.A.; Frontiers in Microbiology; 8; 1992; 11-10-2017; 1-11
1664-302X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2017.01992
info:eu-repo/semantics/altIdentifier/url/journal.frontiersin.org/Journal/10.3389/fmicb.2017.01992/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media S.A.
publisher.none.fl_str_mv Frontiers Media S.A.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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