Anandamide injected into the lateral ventricle of the brain inhibits submandibular salivary secretion by attenuating parasympathetic neurotransmission

Autores
Fernández Solari, José Javier; Prestifilippo, Juan Pablo; Vissio, Paula Gabriela; Ehrhart Bornstein, M.; Bornstein, S. R.; Rettori, V.; Elverdín, Juan Carlos
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Our objective was to determine the effect of arachidonylethanolamide (anandamide, AEA) injected intracerebroventricularly (icv) into the lateral ventricle of the rat brain on submandibular gland (SMG) salivary secretion. Parasympathetic decentralization (PSD) produced by cutting the chorda tympani nerve strongly inhibited methacholine (MC)-induced salivary secretion while sympathetic denervation (SD) produced by removing the superior cervical ganglia reduced it slightly. Also, AEA (50 ng/5 μL, icv) significantly decreased MC-induced salivary secretion in intact rats (MC 1 μg/kg: control (C), 5.3 ± 0.6 vs AEA, 2.7 ± 0.6 mg; MC 3 μg/kg: C, 17.6 ± 1.0 vs AEA, 8.7 ± 0.9 mg; MC 10 μg/kg: C, 37.4 ± 1.2 vs AEA, 22.9 ± 2.6 mg). However, AEA did not alter the significantly reduced salivary secretion in rats with PSD, but decreased the slightly reduced salivary secretion in rats with SD (MC 1 μg/kg: C, 3.8 ± 0.8 vs AEA, 1.4 ± 0.6 mg; MC 3 μg/kg: C, 14.7 ± 2.4 vs AEA, 6.9 ± 1.2 mg; P < 0.05; MC 10 μg/kg: C, 39.5 ± 1.0 vs AEA, 22.3 ± 0.5 mg; P < 0.001). We showed that the inhibitory effect of AEA is mediated by cannabinoid type 1 CB1 receptors and involves GABAergic neurotransmission, since it was blocked by previous injection of the CB1 receptor antagonist AM251 (500 ng/5 μL, icv) or of the GABAA receptor antagonist, bicuculline (25 ng/ 5 μL, icv). Our results suggest that parasympathetic neurotransmission from the central nervous system to the SMG can be inhibited by endocannabinoid and GABAergic systems.
Fil: Fernández Solari, José Javier. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Prestifilippo, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires; Argentina
Fil: Vissio, Paula Gabriela. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ehrhart Bornstein, M.. Technische Universität Dresden; Alemania
Fil: Bornstein, S. R.. Technische Universität Dresden; Alemania
Fil: Rettori, V.. Universidad de Buenos Aires; Argentina
Fil: Elverdín, Juan Carlos. Universidad de Buenos Aires; Argentina
Materia
ANANDAMIDE
CANNABINOID RECEPTOR TYPE 1
GAMMA-AMINOBUTYRIC ACID
PARASYMPATHETIC NERVOUS SYSTEM
SUBMANDIBULAR GLAND
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/117858
Acceder
id CONICETDig_3c978e4f4ed75314c53bc3a9e8360aad
oai_identifier_str oai:ri.conicet.gov.ar:11336/117858
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Anandamide injected into the lateral ventricle of the brain inhibits submandibular salivary secretion by attenuating parasympathetic neurotransmissionFernández Solari, José JavierPrestifilippo, Juan PabloVissio, Paula GabrielaEhrhart Bornstein, M.Bornstein, S. R.Rettori, V.Elverdín, Juan CarlosANANDAMIDECANNABINOID RECEPTOR TYPE 1GAMMA-AMINOBUTYRIC ACIDPARASYMPATHETIC NERVOUS SYSTEMSUBMANDIBULAR GLANDhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Our objective was to determine the effect of arachidonylethanolamide (anandamide, AEA) injected intracerebroventricularly (icv) into the lateral ventricle of the rat brain on submandibular gland (SMG) salivary secretion. Parasympathetic decentralization (PSD) produced by cutting the chorda tympani nerve strongly inhibited methacholine (MC)-induced salivary secretion while sympathetic denervation (SD) produced by removing the superior cervical ganglia reduced it slightly. Also, AEA (50 ng/5 μL, icv) significantly decreased MC-induced salivary secretion in intact rats (MC 1 μg/kg: control (C), 5.3 ± 0.6 vs AEA, 2.7 ± 0.6 mg; MC 3 μg/kg: C, 17.6 ± 1.0 vs AEA, 8.7 ± 0.9 mg; MC 10 μg/kg: C, 37.4 ± 1.2 vs AEA, 22.9 ± 2.6 mg). However, AEA did not alter the significantly reduced salivary secretion in rats with PSD, but decreased the slightly reduced salivary secretion in rats with SD (MC 1 μg/kg: C, 3.8 ± 0.8 vs AEA, 1.4 ± 0.6 mg; MC 3 μg/kg: C, 14.7 ± 2.4 vs AEA, 6.9 ± 1.2 mg; P < 0.05; MC 10 μg/kg: C, 39.5 ± 1.0 vs AEA, 22.3 ± 0.5 mg; P < 0.001). We showed that the inhibitory effect of AEA is mediated by cannabinoid type 1 CB1 receptors and involves GABAergic neurotransmission, since it was blocked by previous injection of the CB1 receptor antagonist AM251 (500 ng/5 μL, icv) or of the GABAA receptor antagonist, bicuculline (25 ng/ 5 μL, icv). Our results suggest that parasympathetic neurotransmission from the central nervous system to the SMG can be inhibited by endocannabinoid and GABAergic systems.Fil: Fernández Solari, José Javier. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Prestifilippo, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires; ArgentinaFil: Vissio, Paula Gabriela. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ehrhart Bornstein, M.. Technische Universität Dresden; AlemaniaFil: Bornstein, S. R.. Technische Universität Dresden; AlemaniaFil: Rettori, V.. Universidad de Buenos Aires; ArgentinaFil: Elverdín, Juan Carlos. Universidad de Buenos Aires; ArgentinaAssociação Brasileira de Divulgação Científica2009-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/117858Fernández Solari, José Javier; Prestifilippo, Juan Pablo; Vissio, Paula Gabriela; Ehrhart Bornstein, M.; Bornstein, S. R.; et al.; Anandamide injected into the lateral ventricle of the brain inhibits submandibular salivary secretion by attenuating parasympathetic neurotransmission; Associação Brasileira de Divulgação Científica; Brazilian Journal of Medical and Biological Research; 42; 6; 12-2009; 537-5440100-879X1414-431XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000600010info:eu-repo/semantics/altIdentifier/doi/10.1590/S0100-879X2009000600010info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:25:33Zoai:ri.conicet.gov.ar:11336/117858instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:25:33.867CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Anandamide injected into the lateral ventricle of the brain inhibits submandibular salivary secretion by attenuating parasympathetic neurotransmission
title Anandamide injected into the lateral ventricle of the brain inhibits submandibular salivary secretion by attenuating parasympathetic neurotransmission
spellingShingle Anandamide injected into the lateral ventricle of the brain inhibits submandibular salivary secretion by attenuating parasympathetic neurotransmission
Fernández Solari, José Javier
ANANDAMIDE
CANNABINOID RECEPTOR TYPE 1
GAMMA-AMINOBUTYRIC ACID
PARASYMPATHETIC NERVOUS SYSTEM
SUBMANDIBULAR GLAND
title_short Anandamide injected into the lateral ventricle of the brain inhibits submandibular salivary secretion by attenuating parasympathetic neurotransmission
title_full Anandamide injected into the lateral ventricle of the brain inhibits submandibular salivary secretion by attenuating parasympathetic neurotransmission
title_fullStr Anandamide injected into the lateral ventricle of the brain inhibits submandibular salivary secretion by attenuating parasympathetic neurotransmission
title_full_unstemmed Anandamide injected into the lateral ventricle of the brain inhibits submandibular salivary secretion by attenuating parasympathetic neurotransmission
title_sort Anandamide injected into the lateral ventricle of the brain inhibits submandibular salivary secretion by attenuating parasympathetic neurotransmission
dc.creator.none.fl_str_mv Fernández Solari, José Javier
Prestifilippo, Juan Pablo
Vissio, Paula Gabriela
Ehrhart Bornstein, M.
Bornstein, S. R.
Rettori, V.
Elverdín, Juan Carlos
author Fernández Solari, José Javier
author_facet Fernández Solari, José Javier
Prestifilippo, Juan Pablo
Vissio, Paula Gabriela
Ehrhart Bornstein, M.
Bornstein, S. R.
Rettori, V.
Elverdín, Juan Carlos
author_role author
author2 Prestifilippo, Juan Pablo
Vissio, Paula Gabriela
Ehrhart Bornstein, M.
Bornstein, S. R.
Rettori, V.
Elverdín, Juan Carlos
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ANANDAMIDE
CANNABINOID RECEPTOR TYPE 1
GAMMA-AMINOBUTYRIC ACID
PARASYMPATHETIC NERVOUS SYSTEM
SUBMANDIBULAR GLAND
topic ANANDAMIDE
CANNABINOID RECEPTOR TYPE 1
GAMMA-AMINOBUTYRIC ACID
PARASYMPATHETIC NERVOUS SYSTEM
SUBMANDIBULAR GLAND
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Our objective was to determine the effect of arachidonylethanolamide (anandamide, AEA) injected intracerebroventricularly (icv) into the lateral ventricle of the rat brain on submandibular gland (SMG) salivary secretion. Parasympathetic decentralization (PSD) produced by cutting the chorda tympani nerve strongly inhibited methacholine (MC)-induced salivary secretion while sympathetic denervation (SD) produced by removing the superior cervical ganglia reduced it slightly. Also, AEA (50 ng/5 μL, icv) significantly decreased MC-induced salivary secretion in intact rats (MC 1 μg/kg: control (C), 5.3 ± 0.6 vs AEA, 2.7 ± 0.6 mg; MC 3 μg/kg: C, 17.6 ± 1.0 vs AEA, 8.7 ± 0.9 mg; MC 10 μg/kg: C, 37.4 ± 1.2 vs AEA, 22.9 ± 2.6 mg). However, AEA did not alter the significantly reduced salivary secretion in rats with PSD, but decreased the slightly reduced salivary secretion in rats with SD (MC 1 μg/kg: C, 3.8 ± 0.8 vs AEA, 1.4 ± 0.6 mg; MC 3 μg/kg: C, 14.7 ± 2.4 vs AEA, 6.9 ± 1.2 mg; P < 0.05; MC 10 μg/kg: C, 39.5 ± 1.0 vs AEA, 22.3 ± 0.5 mg; P < 0.001). We showed that the inhibitory effect of AEA is mediated by cannabinoid type 1 CB1 receptors and involves GABAergic neurotransmission, since it was blocked by previous injection of the CB1 receptor antagonist AM251 (500 ng/5 μL, icv) or of the GABAA receptor antagonist, bicuculline (25 ng/ 5 μL, icv). Our results suggest that parasympathetic neurotransmission from the central nervous system to the SMG can be inhibited by endocannabinoid and GABAergic systems.
Fil: Fernández Solari, José Javier. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Prestifilippo, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires; Argentina
Fil: Vissio, Paula Gabriela. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ehrhart Bornstein, M.. Technische Universität Dresden; Alemania
Fil: Bornstein, S. R.. Technische Universität Dresden; Alemania
Fil: Rettori, V.. Universidad de Buenos Aires; Argentina
Fil: Elverdín, Juan Carlos. Universidad de Buenos Aires; Argentina
description Our objective was to determine the effect of arachidonylethanolamide (anandamide, AEA) injected intracerebroventricularly (icv) into the lateral ventricle of the rat brain on submandibular gland (SMG) salivary secretion. Parasympathetic decentralization (PSD) produced by cutting the chorda tympani nerve strongly inhibited methacholine (MC)-induced salivary secretion while sympathetic denervation (SD) produced by removing the superior cervical ganglia reduced it slightly. Also, AEA (50 ng/5 μL, icv) significantly decreased MC-induced salivary secretion in intact rats (MC 1 μg/kg: control (C), 5.3 ± 0.6 vs AEA, 2.7 ± 0.6 mg; MC 3 μg/kg: C, 17.6 ± 1.0 vs AEA, 8.7 ± 0.9 mg; MC 10 μg/kg: C, 37.4 ± 1.2 vs AEA, 22.9 ± 2.6 mg). However, AEA did not alter the significantly reduced salivary secretion in rats with PSD, but decreased the slightly reduced salivary secretion in rats with SD (MC 1 μg/kg: C, 3.8 ± 0.8 vs AEA, 1.4 ± 0.6 mg; MC 3 μg/kg: C, 14.7 ± 2.4 vs AEA, 6.9 ± 1.2 mg; P < 0.05; MC 10 μg/kg: C, 39.5 ± 1.0 vs AEA, 22.3 ± 0.5 mg; P < 0.001). We showed that the inhibitory effect of AEA is mediated by cannabinoid type 1 CB1 receptors and involves GABAergic neurotransmission, since it was blocked by previous injection of the CB1 receptor antagonist AM251 (500 ng/5 μL, icv) or of the GABAA receptor antagonist, bicuculline (25 ng/ 5 μL, icv). Our results suggest that parasympathetic neurotransmission from the central nervous system to the SMG can be inhibited by endocannabinoid and GABAergic systems.
publishDate 2009
dc.date.none.fl_str_mv 2009-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/117858
Fernández Solari, José Javier; Prestifilippo, Juan Pablo; Vissio, Paula Gabriela; Ehrhart Bornstein, M.; Bornstein, S. R.; et al.; Anandamide injected into the lateral ventricle of the brain inhibits submandibular salivary secretion by attenuating parasympathetic neurotransmission; Associação Brasileira de Divulgação Científica; Brazilian Journal of Medical and Biological Research; 42; 6; 12-2009; 537-544
0100-879X
1414-431X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/117858
identifier_str_mv Fernández Solari, José Javier; Prestifilippo, Juan Pablo; Vissio, Paula Gabriela; Ehrhart Bornstein, M.; Bornstein, S. R.; et al.; Anandamide injected into the lateral ventricle of the brain inhibits submandibular salivary secretion by attenuating parasympathetic neurotransmission; Associação Brasileira de Divulgação Científica; Brazilian Journal of Medical and Biological Research; 42; 6; 12-2009; 537-544
0100-879X
1414-431X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000600010
info:eu-repo/semantics/altIdentifier/doi/10.1590/S0100-879X2009000600010
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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