Reduced methacholine-induced submandibular salivary secretion in rats with experimental periodontitis

Autores
Amer, Mariano; Elverdin, Juan Carlos; Fernández Solari, Jose Javier; Medina, Vanina Araceli; Chiarenza, Ana P.; Vacas, María I.
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
OBJECTIVE: Saliva is the first barrier to the entry of bacteria and viruses into the body and is considered a necessary instrument in oral health. Intraperitoneal injection of lipopolysaccharide endotoxins results in submandibular gland (SMG) hyposalivation. The objective of present studies was to assess if periodontitis, a chronic inflammatory disease caused by oral bacteria, alters cholinergic-induced SMG salivary secretion. DESIGN: An experimental periodontitis model (EP) (cotton thread ligature around the neck of the first lower molars) was used. Male Wistar rats (300-380g) were randomly divided into 3 groups: control, 7 days-bilateral EP and 7 days-unilateral EP (to study if there were different effects at the ipsilateral and contralateral side). The following determinations were performed in SMG: (1) dose-response curves to the cholinergic agonist methacholine, (2) prostaglandin E (PGE) content, (3) inducible nitric oxide synthase (iNOS) activity and (4) histology of gland sections. RESULTS: The molars with EP, no matter the group, exhibited significant and similar bone loss (p<0.001). Bilateral EP reduced methacholine-induced salivary secretion (p<0.05, dose 1μg/kg; p<0.001, dose 3-30μg/kg), increased PGE content (p<0.01), stimulated iNOS activity (p<0.05). Ipsilateral glands of unilateral EP animals presented lower methacholine-induced salivary secretion (p<0.05, dose 3μg/kg; p<0.001, dose 10-30μg/kg), and higher PGE content than contralaterals (p<0.001). In turn, at 3 and 10μg/kg of methacholine, contralateral glands showed significantly lower secretion than control animals (p<0.001). Histological studies of glands revealed partial loss of secretor granular material and periductal oedema in the bilateral and unilateral EP groups as compared to controls. CONCLUSIONS: As far as we know, the present results demonstrate for the first time that EP reduces methacholine-induced SMG salivary secretion.
Fil: Amer, Mariano. Universidad de Buenos Aires. Facultad de Odontología; Argentina
Fil: Elverdin, Juan Carlos. Universidad de Buenos Aires. Facultad de Odontología; Argentina
Fil: Fernández Solari, Jose Javier. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Medina, Vanina Araceli. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chiarenza, Ana P.. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Bioquímica General y Bucal; Argentina
Fil: Vacas, María I.. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Bioquímica General y Bucal; Argentina
Materia
Periodontal Diseases
Submandibular Gland
Salivary Secretion
Inos
Pge
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/16305
Acceder
id CONICETDig_5ecc173ea74c745dbc729da1159aa385
oai_identifier_str oai:ri.conicet.gov.ar:11336/16305
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Reduced methacholine-induced submandibular salivary secretion in rats with experimental periodontitisAmer, MarianoElverdin, Juan CarlosFernández Solari, Jose JavierMedina, Vanina AraceliChiarenza, Ana P.Vacas, María I.Periodontal DiseasesSubmandibular GlandSalivary SecretionInosPgehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3OBJECTIVE: Saliva is the first barrier to the entry of bacteria and viruses into the body and is considered a necessary instrument in oral health. Intraperitoneal injection of lipopolysaccharide endotoxins results in submandibular gland (SMG) hyposalivation. The objective of present studies was to assess if periodontitis, a chronic inflammatory disease caused by oral bacteria, alters cholinergic-induced SMG salivary secretion. DESIGN: An experimental periodontitis model (EP) (cotton thread ligature around the neck of the first lower molars) was used. Male Wistar rats (300-380g) were randomly divided into 3 groups: control, 7 days-bilateral EP and 7 days-unilateral EP (to study if there were different effects at the ipsilateral and contralateral side). The following determinations were performed in SMG: (1) dose-response curves to the cholinergic agonist methacholine, (2) prostaglandin E (PGE) content, (3) inducible nitric oxide synthase (iNOS) activity and (4) histology of gland sections. RESULTS: The molars with EP, no matter the group, exhibited significant and similar bone loss (p<0.001). Bilateral EP reduced methacholine-induced salivary secretion (p<0.05, dose 1μg/kg; p<0.001, dose 3-30μg/kg), increased PGE content (p<0.01), stimulated iNOS activity (p<0.05). Ipsilateral glands of unilateral EP animals presented lower methacholine-induced salivary secretion (p<0.05, dose 3μg/kg; p<0.001, dose 10-30μg/kg), and higher PGE content than contralaterals (p<0.001). In turn, at 3 and 10μg/kg of methacholine, contralateral glands showed significantly lower secretion than control animals (p<0.001). Histological studies of glands revealed partial loss of secretor granular material and periductal oedema in the bilateral and unilateral EP groups as compared to controls. CONCLUSIONS: As far as we know, the present results demonstrate for the first time that EP reduces methacholine-induced SMG salivary secretion.Fil: Amer, Mariano. Universidad de Buenos Aires. Facultad de Odontología; ArgentinaFil: Elverdin, Juan Carlos. Universidad de Buenos Aires. Facultad de Odontología; ArgentinaFil: Fernández Solari, Jose Javier. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Medina, Vanina Araceli. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Chiarenza, Ana P.. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Bioquímica General y Bucal; ArgentinaFil: Vacas, María I.. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Bioquímica General y Bucal; ArgentinaElsevier2011-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/16305Amer, Mariano; Elverdin, Juan Carlos; Fernández Solari, Jose Javier; Medina, Vanina Araceli; Chiarenza, Ana P.; et al.; Reduced methacholine-induced submandibular salivary secretion in rats with experimental periodontitis; Elsevier; Archives of Oral Biology; 56; 5; 5-2011; 421-4270003-9969enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.archoralbio.2010.11.004info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0003996910003390info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T11:25:57Zoai:ri.conicet.gov.ar:11336/16305instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 11:25:58.166CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Reduced methacholine-induced submandibular salivary secretion in rats with experimental periodontitis
title Reduced methacholine-induced submandibular salivary secretion in rats with experimental periodontitis
spellingShingle Reduced methacholine-induced submandibular salivary secretion in rats with experimental periodontitis
Amer, Mariano
Periodontal Diseases
Submandibular Gland
Salivary Secretion
Inos
Pge
title_short Reduced methacholine-induced submandibular salivary secretion in rats with experimental periodontitis
title_full Reduced methacholine-induced submandibular salivary secretion in rats with experimental periodontitis
title_fullStr Reduced methacholine-induced submandibular salivary secretion in rats with experimental periodontitis
title_full_unstemmed Reduced methacholine-induced submandibular salivary secretion in rats with experimental periodontitis
title_sort Reduced methacholine-induced submandibular salivary secretion in rats with experimental periodontitis
dc.creator.none.fl_str_mv Amer, Mariano
Elverdin, Juan Carlos
Fernández Solari, Jose Javier
Medina, Vanina Araceli
Chiarenza, Ana P.
Vacas, María I.
author Amer, Mariano
author_facet Amer, Mariano
Elverdin, Juan Carlos
Fernández Solari, Jose Javier
Medina, Vanina Araceli
Chiarenza, Ana P.
Vacas, María I.
author_role author
author2 Elverdin, Juan Carlos
Fernández Solari, Jose Javier
Medina, Vanina Araceli
Chiarenza, Ana P.
Vacas, María I.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Periodontal Diseases
Submandibular Gland
Salivary Secretion
Inos
Pge
topic Periodontal Diseases
Submandibular Gland
Salivary Secretion
Inos
Pge
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv OBJECTIVE: Saliva is the first barrier to the entry of bacteria and viruses into the body and is considered a necessary instrument in oral health. Intraperitoneal injection of lipopolysaccharide endotoxins results in submandibular gland (SMG) hyposalivation. The objective of present studies was to assess if periodontitis, a chronic inflammatory disease caused by oral bacteria, alters cholinergic-induced SMG salivary secretion. DESIGN: An experimental periodontitis model (EP) (cotton thread ligature around the neck of the first lower molars) was used. Male Wistar rats (300-380g) were randomly divided into 3 groups: control, 7 days-bilateral EP and 7 days-unilateral EP (to study if there were different effects at the ipsilateral and contralateral side). The following determinations were performed in SMG: (1) dose-response curves to the cholinergic agonist methacholine, (2) prostaglandin E (PGE) content, (3) inducible nitric oxide synthase (iNOS) activity and (4) histology of gland sections. RESULTS: The molars with EP, no matter the group, exhibited significant and similar bone loss (p<0.001). Bilateral EP reduced methacholine-induced salivary secretion (p<0.05, dose 1μg/kg; p<0.001, dose 3-30μg/kg), increased PGE content (p<0.01), stimulated iNOS activity (p<0.05). Ipsilateral glands of unilateral EP animals presented lower methacholine-induced salivary secretion (p<0.05, dose 3μg/kg; p<0.001, dose 10-30μg/kg), and higher PGE content than contralaterals (p<0.001). In turn, at 3 and 10μg/kg of methacholine, contralateral glands showed significantly lower secretion than control animals (p<0.001). Histological studies of glands revealed partial loss of secretor granular material and periductal oedema in the bilateral and unilateral EP groups as compared to controls. CONCLUSIONS: As far as we know, the present results demonstrate for the first time that EP reduces methacholine-induced SMG salivary secretion.
Fil: Amer, Mariano. Universidad de Buenos Aires. Facultad de Odontología; Argentina
Fil: Elverdin, Juan Carlos. Universidad de Buenos Aires. Facultad de Odontología; Argentina
Fil: Fernández Solari, Jose Javier. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Medina, Vanina Araceli. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chiarenza, Ana P.. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Bioquímica General y Bucal; Argentina
Fil: Vacas, María I.. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Bioquímica General y Bucal; Argentina
description OBJECTIVE: Saliva is the first barrier to the entry of bacteria and viruses into the body and is considered a necessary instrument in oral health. Intraperitoneal injection of lipopolysaccharide endotoxins results in submandibular gland (SMG) hyposalivation. The objective of present studies was to assess if periodontitis, a chronic inflammatory disease caused by oral bacteria, alters cholinergic-induced SMG salivary secretion. DESIGN: An experimental periodontitis model (EP) (cotton thread ligature around the neck of the first lower molars) was used. Male Wistar rats (300-380g) were randomly divided into 3 groups: control, 7 days-bilateral EP and 7 days-unilateral EP (to study if there were different effects at the ipsilateral and contralateral side). The following determinations were performed in SMG: (1) dose-response curves to the cholinergic agonist methacholine, (2) prostaglandin E (PGE) content, (3) inducible nitric oxide synthase (iNOS) activity and (4) histology of gland sections. RESULTS: The molars with EP, no matter the group, exhibited significant and similar bone loss (p<0.001). Bilateral EP reduced methacholine-induced salivary secretion (p<0.05, dose 1μg/kg; p<0.001, dose 3-30μg/kg), increased PGE content (p<0.01), stimulated iNOS activity (p<0.05). Ipsilateral glands of unilateral EP animals presented lower methacholine-induced salivary secretion (p<0.05, dose 3μg/kg; p<0.001, dose 10-30μg/kg), and higher PGE content than contralaterals (p<0.001). In turn, at 3 and 10μg/kg of methacholine, contralateral glands showed significantly lower secretion than control animals (p<0.001). Histological studies of glands revealed partial loss of secretor granular material and periductal oedema in the bilateral and unilateral EP groups as compared to controls. CONCLUSIONS: As far as we know, the present results demonstrate for the first time that EP reduces methacholine-induced SMG salivary secretion.
publishDate 2011
dc.date.none.fl_str_mv 2011-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/16305
Amer, Mariano; Elverdin, Juan Carlos; Fernández Solari, Jose Javier; Medina, Vanina Araceli; Chiarenza, Ana P.; et al.; Reduced methacholine-induced submandibular salivary secretion in rats with experimental periodontitis; Elsevier; Archives of Oral Biology; 56; 5; 5-2011; 421-427
0003-9969
url http://hdl.handle.net/11336/16305
identifier_str_mv Amer, Mariano; Elverdin, Juan Carlos; Fernández Solari, Jose Javier; Medina, Vanina Araceli; Chiarenza, Ana P.; et al.; Reduced methacholine-induced submandibular salivary secretion in rats with experimental periodontitis; Elsevier; Archives of Oral Biology; 56; 5; 5-2011; 421-427
0003-9969
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.archoralbio.2010.11.004
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0003996910003390
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.001348

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