Hydroxylamido–amino acid complexes of oxovanadium(v). Toxicological study in cell culture and in a zebrafish model

Autores
Leon, Ignacio Esteban; Di Virgilio, Ana Laura; Barrio, Daniel Alejandro; Arrambide, Gabriel; Gambino, Dinorah; Etcheverry, Susana Beatriz
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Oxovanadium(v) complexes [VO(NH2O)2(val)] and [VO(NH2O)2(met)] caused inhibition of cell proliferation in two osteoblast cell lines, MC3T3-E1 and UMR106, as well as the viability of zebrafish eggs. In MC3T3-E1, both compounds inhibited cell proliferation (up to ca. 40% at 25 μM [VO(NH2O)2(val)] and 25% at 25 μM [VO(NH2O)2(met)]). This effect occurs in a dose response manner from 2.5 μM (p < 0.01) with a more deleterious action of [VO(NH2O)2(met)]. In UMR106 tumoral cells, [VO(NH2O)2(val)] inhibited cell proliferation up to 75% from 25 μM while [VO(NH2O)2(met)] behaved as an inhibitory agent in the whole range of concentrations (p < 0.01). Similar toxic effects were obtained from morphological studies in cell cultures. Moreover, the IC50 values for both complexes in culture studies correlated with the IC50 values obtained with an in vivo model of toxicity (FET test). Besides, the cytotoxicity evaluation in cell culture showed a decrease in mitochondrial activity which was stronger for [VO(NH2O)2(met)] than for [VO(NH2O)2(val)] (44% vs. 58% at 25 μM) in both cell lines (p < 0.001). Genotoxicity assessed by micronuclei induction also showed a stronger effect of [VO(NH2O)2(met)] in both cell lines. Besides, [VO(NH2O)2(val)] caused DNA damage determined by comet formation in MC3T3-E1 cells in the range of 2.5–25 μM, while this effect could not be observed in the osteosarcoma cells. On the other hand, [VO(NH2O)2(val)] enhanced ROS levels over basal up to 225% and 170% at 100 μM in MC3T3-E1 and UMR106 cells, respectively (p < 0.01). For [VO(NH2O)2(met)] a similar situation was observed, suggesting an important role for oxidative stress in the toxicity mechanism of action. Although both complexes showed interesting results that would deserve further drug development [VO(NH2O)2(val)] was more stable than [VO(NH2O)2(met)] in the solid state. Therefore, we consider that [VO(NH2O)2(val)] is a good candidate to be tested in in vivo models as a potential antitumoral agent.
Fil: Leon, Ignacio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; Argentina
Fil: Di Virgilio, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; Argentina
Fil: Barrio, Daniel Alejandro. Universidad Nacional de Río Negro. Sede Atlántica; Argentina
Fil: Arrambide, Gabriel. Universidad de la República; Uruguay
Fil: Gambino, Dinorah. Universidad de la República; Uruguay
Fil: Etcheverry, Susana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; Argentina
Materia
Hydroxilamido/aminoacid complexes
Oxovanadium(V)
Cell culture
Zebra fish
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/275117
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/275117
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network_name_str CONICET Digital (CONICET)
spelling Hydroxylamido–amino acid complexes of oxovanadium(v). Toxicological study in cell culture and in a zebrafish modelLeon, Ignacio EstebanDi Virgilio, Ana LauraBarrio, Daniel AlejandroArrambide, GabrielGambino, DinorahEtcheverry, Susana BeatrizHydroxilamido/aminoacid complexesOxovanadium(V)Cell cultureZebra fishhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Oxovanadium(v) complexes [VO(NH2O)2(val)] and [VO(NH2O)2(met)] caused inhibition of cell proliferation in two osteoblast cell lines, MC3T3-E1 and UMR106, as well as the viability of zebrafish eggs. In MC3T3-E1, both compounds inhibited cell proliferation (up to ca. 40% at 25 μM [VO(NH2O)2(val)] and 25% at 25 μM [VO(NH2O)2(met)]). This effect occurs in a dose response manner from 2.5 μM (p < 0.01) with a more deleterious action of [VO(NH2O)2(met)]. In UMR106 tumoral cells, [VO(NH2O)2(val)] inhibited cell proliferation up to 75% from 25 μM while [VO(NH2O)2(met)] behaved as an inhibitory agent in the whole range of concentrations (p < 0.01). Similar toxic effects were obtained from morphological studies in cell cultures. Moreover, the IC50 values for both complexes in culture studies correlated with the IC50 values obtained with an in vivo model of toxicity (FET test). Besides, the cytotoxicity evaluation in cell culture showed a decrease in mitochondrial activity which was stronger for [VO(NH2O)2(met)] than for [VO(NH2O)2(val)] (44% vs. 58% at 25 μM) in both cell lines (p < 0.001). Genotoxicity assessed by micronuclei induction also showed a stronger effect of [VO(NH2O)2(met)] in both cell lines. Besides, [VO(NH2O)2(val)] caused DNA damage determined by comet formation in MC3T3-E1 cells in the range of 2.5–25 μM, while this effect could not be observed in the osteosarcoma cells. On the other hand, [VO(NH2O)2(val)] enhanced ROS levels over basal up to 225% and 170% at 100 μM in MC3T3-E1 and UMR106 cells, respectively (p < 0.01). For [VO(NH2O)2(met)] a similar situation was observed, suggesting an important role for oxidative stress in the toxicity mechanism of action. Although both complexes showed interesting results that would deserve further drug development [VO(NH2O)2(val)] was more stable than [VO(NH2O)2(met)] in the solid state. Therefore, we consider that [VO(NH2O)2(val)] is a good candidate to be tested in in vivo models as a potential antitumoral agent.Fil: Leon, Ignacio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; ArgentinaFil: Di Virgilio, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; ArgentinaFil: Barrio, Daniel Alejandro. Universidad Nacional de Río Negro. Sede Atlántica; ArgentinaFil: Arrambide, Gabriel. Universidad de la República; UruguayFil: Gambino, Dinorah. Universidad de la República; UruguayFil: Etcheverry, Susana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; ArgentinaRoyal Society of Chemistry2012-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/275117Leon, Ignacio Esteban; Di Virgilio, Ana Laura; Barrio, Daniel Alejandro; Arrambide, Gabriel; Gambino, Dinorah; et al.; Hydroxylamido–amino acid complexes of oxovanadium(v). Toxicological study in cell culture and in a zebrafish model; Royal Society of Chemistry; Metallomics; 4; 12; 3-2012; 1287-12961756-5901CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/metallomics/article-abstract/4/12/1287/6016036info:eu-repo/semantics/altIdentifier/doi/10.1039/c2mt20091kinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-03T08:51:40Zoai:ri.conicet.gov.ar:11336/275117instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-03 08:51:41.142CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Hydroxylamido–amino acid complexes of oxovanadium(v). Toxicological study in cell culture and in a zebrafish model
title Hydroxylamido–amino acid complexes of oxovanadium(v). Toxicological study in cell culture and in a zebrafish model
spellingShingle Hydroxylamido–amino acid complexes of oxovanadium(v). Toxicological study in cell culture and in a zebrafish model
Leon, Ignacio Esteban
Hydroxilamido/aminoacid complexes
Oxovanadium(V)
Cell culture
Zebra fish
title_short Hydroxylamido–amino acid complexes of oxovanadium(v). Toxicological study in cell culture and in a zebrafish model
title_full Hydroxylamido–amino acid complexes of oxovanadium(v). Toxicological study in cell culture and in a zebrafish model
title_fullStr Hydroxylamido–amino acid complexes of oxovanadium(v). Toxicological study in cell culture and in a zebrafish model
title_full_unstemmed Hydroxylamido–amino acid complexes of oxovanadium(v). Toxicological study in cell culture and in a zebrafish model
title_sort Hydroxylamido–amino acid complexes of oxovanadium(v). Toxicological study in cell culture and in a zebrafish model
dc.creator.none.fl_str_mv Leon, Ignacio Esteban
Di Virgilio, Ana Laura
Barrio, Daniel Alejandro
Arrambide, Gabriel
Gambino, Dinorah
Etcheverry, Susana Beatriz
author Leon, Ignacio Esteban
author_facet Leon, Ignacio Esteban
Di Virgilio, Ana Laura
Barrio, Daniel Alejandro
Arrambide, Gabriel
Gambino, Dinorah
Etcheverry, Susana Beatriz
author_role author
author2 Di Virgilio, Ana Laura
Barrio, Daniel Alejandro
Arrambide, Gabriel
Gambino, Dinorah
Etcheverry, Susana Beatriz
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Hydroxilamido/aminoacid complexes
Oxovanadium(V)
Cell culture
Zebra fish
topic Hydroxilamido/aminoacid complexes
Oxovanadium(V)
Cell culture
Zebra fish
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Oxovanadium(v) complexes [VO(NH2O)2(val)] and [VO(NH2O)2(met)] caused inhibition of cell proliferation in two osteoblast cell lines, MC3T3-E1 and UMR106, as well as the viability of zebrafish eggs. In MC3T3-E1, both compounds inhibited cell proliferation (up to ca. 40% at 25 μM [VO(NH2O)2(val)] and 25% at 25 μM [VO(NH2O)2(met)]). This effect occurs in a dose response manner from 2.5 μM (p < 0.01) with a more deleterious action of [VO(NH2O)2(met)]. In UMR106 tumoral cells, [VO(NH2O)2(val)] inhibited cell proliferation up to 75% from 25 μM while [VO(NH2O)2(met)] behaved as an inhibitory agent in the whole range of concentrations (p < 0.01). Similar toxic effects were obtained from morphological studies in cell cultures. Moreover, the IC50 values for both complexes in culture studies correlated with the IC50 values obtained with an in vivo model of toxicity (FET test). Besides, the cytotoxicity evaluation in cell culture showed a decrease in mitochondrial activity which was stronger for [VO(NH2O)2(met)] than for [VO(NH2O)2(val)] (44% vs. 58% at 25 μM) in both cell lines (p < 0.001). Genotoxicity assessed by micronuclei induction also showed a stronger effect of [VO(NH2O)2(met)] in both cell lines. Besides, [VO(NH2O)2(val)] caused DNA damage determined by comet formation in MC3T3-E1 cells in the range of 2.5–25 μM, while this effect could not be observed in the osteosarcoma cells. On the other hand, [VO(NH2O)2(val)] enhanced ROS levels over basal up to 225% and 170% at 100 μM in MC3T3-E1 and UMR106 cells, respectively (p < 0.01). For [VO(NH2O)2(met)] a similar situation was observed, suggesting an important role for oxidative stress in the toxicity mechanism of action. Although both complexes showed interesting results that would deserve further drug development [VO(NH2O)2(val)] was more stable than [VO(NH2O)2(met)] in the solid state. Therefore, we consider that [VO(NH2O)2(val)] is a good candidate to be tested in in vivo models as a potential antitumoral agent.
Fil: Leon, Ignacio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; Argentina
Fil: Di Virgilio, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; Argentina
Fil: Barrio, Daniel Alejandro. Universidad Nacional de Río Negro. Sede Atlántica; Argentina
Fil: Arrambide, Gabriel. Universidad de la República; Uruguay
Fil: Gambino, Dinorah. Universidad de la República; Uruguay
Fil: Etcheverry, Susana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; Argentina
description Oxovanadium(v) complexes [VO(NH2O)2(val)] and [VO(NH2O)2(met)] caused inhibition of cell proliferation in two osteoblast cell lines, MC3T3-E1 and UMR106, as well as the viability of zebrafish eggs. In MC3T3-E1, both compounds inhibited cell proliferation (up to ca. 40% at 25 μM [VO(NH2O)2(val)] and 25% at 25 μM [VO(NH2O)2(met)]). This effect occurs in a dose response manner from 2.5 μM (p < 0.01) with a more deleterious action of [VO(NH2O)2(met)]. In UMR106 tumoral cells, [VO(NH2O)2(val)] inhibited cell proliferation up to 75% from 25 μM while [VO(NH2O)2(met)] behaved as an inhibitory agent in the whole range of concentrations (p < 0.01). Similar toxic effects were obtained from morphological studies in cell cultures. Moreover, the IC50 values for both complexes in culture studies correlated with the IC50 values obtained with an in vivo model of toxicity (FET test). Besides, the cytotoxicity evaluation in cell culture showed a decrease in mitochondrial activity which was stronger for [VO(NH2O)2(met)] than for [VO(NH2O)2(val)] (44% vs. 58% at 25 μM) in both cell lines (p < 0.001). Genotoxicity assessed by micronuclei induction also showed a stronger effect of [VO(NH2O)2(met)] in both cell lines. Besides, [VO(NH2O)2(val)] caused DNA damage determined by comet formation in MC3T3-E1 cells in the range of 2.5–25 μM, while this effect could not be observed in the osteosarcoma cells. On the other hand, [VO(NH2O)2(val)] enhanced ROS levels over basal up to 225% and 170% at 100 μM in MC3T3-E1 and UMR106 cells, respectively (p < 0.01). For [VO(NH2O)2(met)] a similar situation was observed, suggesting an important role for oxidative stress in the toxicity mechanism of action. Although both complexes showed interesting results that would deserve further drug development [VO(NH2O)2(val)] was more stable than [VO(NH2O)2(met)] in the solid state. Therefore, we consider that [VO(NH2O)2(val)] is a good candidate to be tested in in vivo models as a potential antitumoral agent.
publishDate 2012
dc.date.none.fl_str_mv 2012-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/275117
Leon, Ignacio Esteban; Di Virgilio, Ana Laura; Barrio, Daniel Alejandro; Arrambide, Gabriel; Gambino, Dinorah; et al.; Hydroxylamido–amino acid complexes of oxovanadium(v). Toxicological study in cell culture and in a zebrafish model; Royal Society of Chemistry; Metallomics; 4; 12; 3-2012; 1287-1296
1756-5901
CONICET Digital
CONICET
url http://hdl.handle.net/11336/275117
identifier_str_mv Leon, Ignacio Esteban; Di Virgilio, Ana Laura; Barrio, Daniel Alejandro; Arrambide, Gabriel; Gambino, Dinorah; et al.; Hydroxylamido–amino acid complexes of oxovanadium(v). Toxicological study in cell culture and in a zebrafish model; Royal Society of Chemistry; Metallomics; 4; 12; 3-2012; 1287-1296
1756-5901
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/metallomics/article-abstract/4/12/1287/6016036
info:eu-repo/semantics/altIdentifier/doi/10.1039/c2mt20091k
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
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dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.275514

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