Optogenetic activation of olivocochlear efferent fibers fibers: In the quest for the source of GABA

Autores
Castagnola, Tais; Elgoyhen, Ana Belen; Goutman, Juan Diego; Wedemeyer, Carolina
Año de publicación
2020
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
During development, inner hair cells (IHCs) in the mammalian cochlea are unresponsive to acoustic stimuli but instead present intrinsic electrical activity, crucial for the normal development of the auditory pathway. During this same period, neurons originating from the medial olivocochlear complex (MOC) transiently innervate IHCs. This innervation is mediated by acetylcholine (ACh), activating nicotinic receptors assembled by α9 and α10 subunits and is responsible for controlling IHC excitability during this period. Even though this is a cholinergic synapse, previous evidence indicates the presence of abundant GABA and presynaptic GABAB receptors. Moreover, the application of GABAB receptors agonists can reduce ACh release. To determine the source of GABA in the MOC ? IHC synapse, transgenic mice expressing channelrodhopsin (ChR2) in GABAergic and cholinergic fibers were used. We show here for the first time, that MOC fibers can be optogenetically activated in ChAT-Cre/ChR2 mice (n=3). In addition, immunohistochemistry techniques were used to characterize expression in these transgenic mice. On the other hand, to further understand the mechanisms of GABA modulation we used calcium imaging techniques that allowed us to estimate activity at a single synapse level. Altogether these results suggest that ACh might be released from fibers that have a GABAergic identity.
Fil: Castagnola, Tais. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Elgoyhen, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacologia; Argentina
Fil: Goutman, Juan Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Wedemeyer, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
XXXV Annual Virtual Meeting of the Argentinian Society of Neuroscience
Ciudad Autónoma de Buenos Aires
Argentina
Sociedad Argentina de Neurociencia
Materia
OLIVOCOCHLEAR EFFERENT SYSTEM
OPTOGENETICS
EFFERENT
GABA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/201731
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Optogenetic activation of olivocochlear efferent fibers fibers: In the quest for the source of GABACastagnola, TaisElgoyhen, Ana BelenGoutman, Juan DiegoWedemeyer, CarolinaOLIVOCOCHLEAR EFFERENT SYSTEMOPTOGENETICSEFFERENTGABAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1During development, inner hair cells (IHCs) in the mammalian cochlea are unresponsive to acoustic stimuli but instead present intrinsic electrical activity, crucial for the normal development of the auditory pathway. During this same period, neurons originating from the medial olivocochlear complex (MOC) transiently innervate IHCs. This innervation is mediated by acetylcholine (ACh), activating nicotinic receptors assembled by α9 and α10 subunits and is responsible for controlling IHC excitability during this period. Even though this is a cholinergic synapse, previous evidence indicates the presence of abundant GABA and presynaptic GABAB receptors. Moreover, the application of GABAB receptors agonists can reduce ACh release. To determine the source of GABA in the MOC ? IHC synapse, transgenic mice expressing channelrodhopsin (ChR2) in GABAergic and cholinergic fibers were used. We show here for the first time, that MOC fibers can be optogenetically activated in ChAT-Cre/ChR2 mice (n=3). In addition, immunohistochemistry techniques were used to characterize expression in these transgenic mice. On the other hand, to further understand the mechanisms of GABA modulation we used calcium imaging techniques that allowed us to estimate activity at a single synapse level. Altogether these results suggest that ACh might be released from fibers that have a GABAergic identity.Fil: Castagnola, Tais. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Elgoyhen, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacologia; ArgentinaFil: Goutman, Juan Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Wedemeyer, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaXXXV Annual Virtual Meeting of the Argentinian Society of NeuroscienceCiudad Autónoma de Buenos AiresArgentinaSociedad Argentina de NeurocienciaSociedad Argentina de Neurociencias2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/201731Optogenetic activation of olivocochlear efferent fibers fibers: In the quest for the source of GABA; XXXV Annual Virtual Meeting of the Argentinian Society of Neuroscience; Ciudad Autónoma de Buenos Aires; Argentina; 2020; 329-329CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://csan2020.saneurociencias.org.ar/wp-content/uploads/2021/06/EBOOK-2020.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:05:11Zoai:ri.conicet.gov.ar:11336/201731instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:05:11.616CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Optogenetic activation of olivocochlear efferent fibers fibers: In the quest for the source of GABA
title Optogenetic activation of olivocochlear efferent fibers fibers: In the quest for the source of GABA
spellingShingle Optogenetic activation of olivocochlear efferent fibers fibers: In the quest for the source of GABA
Castagnola, Tais
OLIVOCOCHLEAR EFFERENT SYSTEM
OPTOGENETICS
EFFERENT
GABA
title_short Optogenetic activation of olivocochlear efferent fibers fibers: In the quest for the source of GABA
title_full Optogenetic activation of olivocochlear efferent fibers fibers: In the quest for the source of GABA
title_fullStr Optogenetic activation of olivocochlear efferent fibers fibers: In the quest for the source of GABA
title_full_unstemmed Optogenetic activation of olivocochlear efferent fibers fibers: In the quest for the source of GABA
title_sort Optogenetic activation of olivocochlear efferent fibers fibers: In the quest for the source of GABA
dc.creator.none.fl_str_mv Castagnola, Tais
Elgoyhen, Ana Belen
Goutman, Juan Diego
Wedemeyer, Carolina
author Castagnola, Tais
author_facet Castagnola, Tais
Elgoyhen, Ana Belen
Goutman, Juan Diego
Wedemeyer, Carolina
author_role author
author2 Elgoyhen, Ana Belen
Goutman, Juan Diego
Wedemeyer, Carolina
author2_role author
author
author
dc.subject.none.fl_str_mv OLIVOCOCHLEAR EFFERENT SYSTEM
OPTOGENETICS
EFFERENT
GABA
topic OLIVOCOCHLEAR EFFERENT SYSTEM
OPTOGENETICS
EFFERENT
GABA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv During development, inner hair cells (IHCs) in the mammalian cochlea are unresponsive to acoustic stimuli but instead present intrinsic electrical activity, crucial for the normal development of the auditory pathway. During this same period, neurons originating from the medial olivocochlear complex (MOC) transiently innervate IHCs. This innervation is mediated by acetylcholine (ACh), activating nicotinic receptors assembled by α9 and α10 subunits and is responsible for controlling IHC excitability during this period. Even though this is a cholinergic synapse, previous evidence indicates the presence of abundant GABA and presynaptic GABAB receptors. Moreover, the application of GABAB receptors agonists can reduce ACh release. To determine the source of GABA in the MOC ? IHC synapse, transgenic mice expressing channelrodhopsin (ChR2) in GABAergic and cholinergic fibers were used. We show here for the first time, that MOC fibers can be optogenetically activated in ChAT-Cre/ChR2 mice (n=3). In addition, immunohistochemistry techniques were used to characterize expression in these transgenic mice. On the other hand, to further understand the mechanisms of GABA modulation we used calcium imaging techniques that allowed us to estimate activity at a single synapse level. Altogether these results suggest that ACh might be released from fibers that have a GABAergic identity.
Fil: Castagnola, Tais. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Elgoyhen, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacologia; Argentina
Fil: Goutman, Juan Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Wedemeyer, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
XXXV Annual Virtual Meeting of the Argentinian Society of Neuroscience
Ciudad Autónoma de Buenos Aires
Argentina
Sociedad Argentina de Neurociencia
description During development, inner hair cells (IHCs) in the mammalian cochlea are unresponsive to acoustic stimuli but instead present intrinsic electrical activity, crucial for the normal development of the auditory pathway. During this same period, neurons originating from the medial olivocochlear complex (MOC) transiently innervate IHCs. This innervation is mediated by acetylcholine (ACh), activating nicotinic receptors assembled by α9 and α10 subunits and is responsible for controlling IHC excitability during this period. Even though this is a cholinergic synapse, previous evidence indicates the presence of abundant GABA and presynaptic GABAB receptors. Moreover, the application of GABAB receptors agonists can reduce ACh release. To determine the source of GABA in the MOC ? IHC synapse, transgenic mice expressing channelrodhopsin (ChR2) in GABAergic and cholinergic fibers were used. We show here for the first time, that MOC fibers can be optogenetically activated in ChAT-Cre/ChR2 mice (n=3). In addition, immunohistochemistry techniques were used to characterize expression in these transgenic mice. On the other hand, to further understand the mechanisms of GABA modulation we used calcium imaging techniques that allowed us to estimate activity at a single synapse level. Altogether these results suggest that ACh might be released from fibers that have a GABAergic identity.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/201731
Optogenetic activation of olivocochlear efferent fibers fibers: In the quest for the source of GABA; XXXV Annual Virtual Meeting of the Argentinian Society of Neuroscience; Ciudad Autónoma de Buenos Aires; Argentina; 2020; 329-329
CONICET Digital
CONICET
url http://hdl.handle.net/11336/201731
identifier_str_mv Optogenetic activation of olivocochlear efferent fibers fibers: In the quest for the source of GABA; XXXV Annual Virtual Meeting of the Argentinian Society of Neuroscience; Ciudad Autónoma de Buenos Aires; Argentina; 2020; 329-329
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://csan2020.saneurociencias.org.ar/wp-content/uploads/2021/06/EBOOK-2020.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Neurociencias
publisher.none.fl_str_mv Sociedad Argentina de Neurociencias
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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