GABA and ACh are co-released from olivocochlear efferent terminals

Autores
Castagnola, Tais; Elgoyhen, Ana Belen; Gomez Casati, Maria Eugenia; Castagna, Valeria Carolina; Goutman, Juan Diego; Wedemeyer, Carolina
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
During development, inner hair cells (IHCs) in the mammalian cochlea are unresponsive to acoustic stimuli but instead present intrinsic electrical activity, crucial for the normal development of the auditory pathway. During this same period, neurons originating from the medial olivocochlear complex (MOC) transiently innervate IHCs. This innervation is mediated by acetylcholine (ACh), activating nicotinic receptors assembled by α9 and α10 subunits and is responsible for controlling IHC excitability during this period. Even though this is a cholinergic synapse, previous evidence indicates the presence of abundant GABA and GABAB receptors in MOC fibers in the inner spiral bundle. Moreover, the application of GABAB agonists reduces ACh release. Transgenic mice expressing channelrodhopsin (ChR2) under the control of either GAD or ChAT promoters were used in this study. Here we show for the first time, that optogenetically activated fibers in GAD-cre/ChR2 mice (n=7) produced postsynaptic responses that were blocked with cholinergic antagonists (n=3). In addition, pharmacological experiments indicate GABAB activation in ChAT-cre/ChR2 mice (n=4). ChAT-cre/TdTomato cochleas, co-stained with antibody against GAD, showed a co-localization of GABAergic and cholinergic terminals in the inner spiral bundle. Altogether these results strongly suggest that ACh is being co-released with GABA from MOC fibers.
Fil: Castagnola, Tais. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Elgoyhen, Ana Belen. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Gomez Casati, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacología; Argentina
Fil: Castagna, Valeria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacología; Argentina
Fil: Goutman, Juan Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Wedemeyer, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
XXXVII Annual Meeting of the Argentine Society for Research in Neuroscience
Ciudad Autónoma de Buenos Aires
Argentina
Sociedad Argentina de Investigación en Neurociencias
Materia
COCHLEA
EFFERENTS
OLIVOCOCHLEAR
OPTOGENETIC
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/228276
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/228276
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling GABA and ACh are co-released from olivocochlear efferent terminalsCastagnola, TaisElgoyhen, Ana BelenGomez Casati, Maria EugeniaCastagna, Valeria CarolinaGoutman, Juan DiegoWedemeyer, CarolinaCOCHLEAEFFERENTSOLIVOCOCHLEAROPTOGENETIChttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1During development, inner hair cells (IHCs) in the mammalian cochlea are unresponsive to acoustic stimuli but instead present intrinsic electrical activity, crucial for the normal development of the auditory pathway. During this same period, neurons originating from the medial olivocochlear complex (MOC) transiently innervate IHCs. This innervation is mediated by acetylcholine (ACh), activating nicotinic receptors assembled by α9 and α10 subunits and is responsible for controlling IHC excitability during this period. Even though this is a cholinergic synapse, previous evidence indicates the presence of abundant GABA and GABAB receptors in MOC fibers in the inner spiral bundle. Moreover, the application of GABAB agonists reduces ACh release. Transgenic mice expressing channelrodhopsin (ChR2) under the control of either GAD or ChAT promoters were used in this study. Here we show for the first time, that optogenetically activated fibers in GAD-cre/ChR2 mice (n=7) produced postsynaptic responses that were blocked with cholinergic antagonists (n=3). In addition, pharmacological experiments indicate GABAB activation in ChAT-cre/ChR2 mice (n=4). ChAT-cre/TdTomato cochleas, co-stained with antibody against GAD, showed a co-localization of GABAergic and cholinergic terminals in the inner spiral bundle. Altogether these results strongly suggest that ACh is being co-released with GABA from MOC fibers.Fil: Castagnola, Tais. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Elgoyhen, Ana Belen. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Gomez Casati, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacología; ArgentinaFil: Castagna, Valeria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacología; ArgentinaFil: Goutman, Juan Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Wedemeyer, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaXXXVII Annual Meeting of the Argentine Society for Research in NeuroscienceCiudad Autónoma de Buenos AiresArgentinaSociedad Argentina de Investigación en NeurocienciasSociedad Argentina de Investigación en Neurociencias2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/228276GABA and ACh are co-released from olivocochlear efferent terminals; XXXVII Annual Meeting of the Argentine Society for Research in Neuroscience; Ciudad Autónoma de Buenos Aires; Argentina; 2021; 231-231CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://csan2021.saneurociencias.org.ar/Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:38:56Zoai:ri.conicet.gov.ar:11336/228276instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:38:56.978CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv GABA and ACh are co-released from olivocochlear efferent terminals
title GABA and ACh are co-released from olivocochlear efferent terminals
spellingShingle GABA and ACh are co-released from olivocochlear efferent terminals
Castagnola, Tais
COCHLEA
EFFERENTS
OLIVOCOCHLEAR
OPTOGENETIC
title_short GABA and ACh are co-released from olivocochlear efferent terminals
title_full GABA and ACh are co-released from olivocochlear efferent terminals
title_fullStr GABA and ACh are co-released from olivocochlear efferent terminals
title_full_unstemmed GABA and ACh are co-released from olivocochlear efferent terminals
title_sort GABA and ACh are co-released from olivocochlear efferent terminals
dc.creator.none.fl_str_mv Castagnola, Tais
Elgoyhen, Ana Belen
Gomez Casati, Maria Eugenia
Castagna, Valeria Carolina
Goutman, Juan Diego
Wedemeyer, Carolina
author Castagnola, Tais
author_facet Castagnola, Tais
Elgoyhen, Ana Belen
Gomez Casati, Maria Eugenia
Castagna, Valeria Carolina
Goutman, Juan Diego
Wedemeyer, Carolina
author_role author
author2 Elgoyhen, Ana Belen
Gomez Casati, Maria Eugenia
Castagna, Valeria Carolina
Goutman, Juan Diego
Wedemeyer, Carolina
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv COCHLEA
EFFERENTS
OLIVOCOCHLEAR
OPTOGENETIC
topic COCHLEA
EFFERENTS
OLIVOCOCHLEAR
OPTOGENETIC
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv During development, inner hair cells (IHCs) in the mammalian cochlea are unresponsive to acoustic stimuli but instead present intrinsic electrical activity, crucial for the normal development of the auditory pathway. During this same period, neurons originating from the medial olivocochlear complex (MOC) transiently innervate IHCs. This innervation is mediated by acetylcholine (ACh), activating nicotinic receptors assembled by α9 and α10 subunits and is responsible for controlling IHC excitability during this period. Even though this is a cholinergic synapse, previous evidence indicates the presence of abundant GABA and GABAB receptors in MOC fibers in the inner spiral bundle. Moreover, the application of GABAB agonists reduces ACh release. Transgenic mice expressing channelrodhopsin (ChR2) under the control of either GAD or ChAT promoters were used in this study. Here we show for the first time, that optogenetically activated fibers in GAD-cre/ChR2 mice (n=7) produced postsynaptic responses that were blocked with cholinergic antagonists (n=3). In addition, pharmacological experiments indicate GABAB activation in ChAT-cre/ChR2 mice (n=4). ChAT-cre/TdTomato cochleas, co-stained with antibody against GAD, showed a co-localization of GABAergic and cholinergic terminals in the inner spiral bundle. Altogether these results strongly suggest that ACh is being co-released with GABA from MOC fibers.
Fil: Castagnola, Tais. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Elgoyhen, Ana Belen. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Gomez Casati, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacología; Argentina
Fil: Castagna, Valeria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacología; Argentina
Fil: Goutman, Juan Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Wedemeyer, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
XXXVII Annual Meeting of the Argentine Society for Research in Neuroscience
Ciudad Autónoma de Buenos Aires
Argentina
Sociedad Argentina de Investigación en Neurociencias
description During development, inner hair cells (IHCs) in the mammalian cochlea are unresponsive to acoustic stimuli but instead present intrinsic electrical activity, crucial for the normal development of the auditory pathway. During this same period, neurons originating from the medial olivocochlear complex (MOC) transiently innervate IHCs. This innervation is mediated by acetylcholine (ACh), activating nicotinic receptors assembled by α9 and α10 subunits and is responsible for controlling IHC excitability during this period. Even though this is a cholinergic synapse, previous evidence indicates the presence of abundant GABA and GABAB receptors in MOC fibers in the inner spiral bundle. Moreover, the application of GABAB agonists reduces ACh release. Transgenic mice expressing channelrodhopsin (ChR2) under the control of either GAD or ChAT promoters were used in this study. Here we show for the first time, that optogenetically activated fibers in GAD-cre/ChR2 mice (n=7) produced postsynaptic responses that were blocked with cholinergic antagonists (n=3). In addition, pharmacological experiments indicate GABAB activation in ChAT-cre/ChR2 mice (n=4). ChAT-cre/TdTomato cochleas, co-stained with antibody against GAD, showed a co-localization of GABAergic and cholinergic terminals in the inner spiral bundle. Altogether these results strongly suggest that ACh is being co-released with GABA from MOC fibers.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/228276
GABA and ACh are co-released from olivocochlear efferent terminals; XXXVII Annual Meeting of the Argentine Society for Research in Neuroscience; Ciudad Autónoma de Buenos Aires; Argentina; 2021; 231-231
CONICET Digital
CONICET
url http://hdl.handle.net/11336/228276
identifier_str_mv GABA and ACh are co-released from olivocochlear efferent terminals; XXXVII Annual Meeting of the Argentine Society for Research in Neuroscience; Ciudad Autónoma de Buenos Aires; Argentina; 2021; 231-231
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://csan2021.saneurociencias.org.ar/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Investigación en Neurociencias
publisher.none.fl_str_mv Sociedad Argentina de Investigación en Neurociencias
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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