Potential Bacterial Antagonists for the Control of Charcoal Rot (Macrophomina phaseolina) in Strawberry

Autores
Viejobueno, Josefina; Rodríguez-Berbel, Natalia; Miranda, Luis; Santos, Berta de los; Camacho, María
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The effect of antagonistic bacteria to control Macrophomina phaseolina was evaluated under in vitro, growth chamber, greenhouse and field conditions. A total of 177 bacteria, isolated from Athrocaulon macrostachyum rhizosphere of the Lebrija marsh, were screened for their potential against M. phaseolina (causes charcoal rot in strawberry) by dual culture assay. Of these isolates, 14 most promising strains were molecularly identified by the 16S rDNA sequencing method using the EzBioCloud database. These strains were tested for in vitro hydrolytic enzymes, HCN production, and biocontrol against M. phaseolina in strawberry plants. All the 14 strains produced, at least, one hydrolytic enzymatic activity and one of them, which belongs to Brevibacterium genus (Hvs8), showed the lowest records of disease incidence (20%) and severity (0.4). With these results, greenhouse and field trials were carried out with the Hvs8 strain, compared to non-treated control. In the greenhouse assays, Hvs8 strain increased root dry mass by 30%, over the control. In the field trials, production and fruit quality were not significantly different between Hvs8 treatment and non-treated control, but plant mortality and plant mortality associated to M. phaseolina decreased by more than 24% and 65% respectively, in Hvs8 treatment. This study suggests that Brevibacterium sp. Hvs8 strain could be a candidate for controlling charcoal rot in strawberry.
EEA Famaillá
Fil: Viejobueno, Josefina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Rodríguez-Berbel, Natalia. University of Almeria. Department of Agronomy & Center for Intensive Mediterranean Agronomy and Agri-Food Biotechnology (CIAIMBITAL); España
Fil: Miranda, Luis. Instituto Andaluz de Investigación y Formación Agraria, Pesquera, Alimentaria y de la Producción Ecológica; España
Fil: Santos, Berta de los. Instituto Andaluz de Investigación y Formación Agraria, Pesquera, Alimentaria y de la Producción Ecológica; España
Fil: Camacho, María. Instituto Andaluz de Investigación y Formación Agraria, Pesquera, Alimentaria y de la Producción Ecológica; España
Fuente
Horticulturae 7 (11) : 457. (November 2021)
Materia
Fresa
Enfermedades de las Plantas
Control de Enfermedades de Plantas
Macrophomina phaseolina
Control Biológico
Bacterias Antagonistas
Strawberries
Plant Diseases
Plant Disease Control
Biological Control
Antagonistic Bacteria
Frutilla
Charcoal Rot Disease
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/11797
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oai_identifier_str oai:localhost:20.500.12123/11797
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network_name_str INTA Digital (INTA)
spelling Potential Bacterial Antagonists for the Control of Charcoal Rot (Macrophomina phaseolina) in StrawberryViejobueno, JosefinaRodríguez-Berbel, NataliaMiranda, LuisSantos, Berta de losCamacho, MaríaFresaEnfermedades de las PlantasControl de Enfermedades de PlantasMacrophomina phaseolinaControl BiológicoBacterias AntagonistasStrawberriesPlant DiseasesPlant Disease ControlBiological ControlAntagonistic BacteriaFrutillaCharcoal Rot DiseaseThe effect of antagonistic bacteria to control Macrophomina phaseolina was evaluated under in vitro, growth chamber, greenhouse and field conditions. A total of 177 bacteria, isolated from Athrocaulon macrostachyum rhizosphere of the Lebrija marsh, were screened for their potential against M. phaseolina (causes charcoal rot in strawberry) by dual culture assay. Of these isolates, 14 most promising strains were molecularly identified by the 16S rDNA sequencing method using the EzBioCloud database. These strains were tested for in vitro hydrolytic enzymes, HCN production, and biocontrol against M. phaseolina in strawberry plants. All the 14 strains produced, at least, one hydrolytic enzymatic activity and one of them, which belongs to Brevibacterium genus (Hvs8), showed the lowest records of disease incidence (20%) and severity (0.4). With these results, greenhouse and field trials were carried out with the Hvs8 strain, compared to non-treated control. In the greenhouse assays, Hvs8 strain increased root dry mass by 30%, over the control. In the field trials, production and fruit quality were not significantly different between Hvs8 treatment and non-treated control, but plant mortality and plant mortality associated to M. phaseolina decreased by more than 24% and 65% respectively, in Hvs8 treatment. This study suggests that Brevibacterium sp. Hvs8 strain could be a candidate for controlling charcoal rot in strawberry.EEA FamailláFil: Viejobueno, Josefina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; ArgentinaFil: Rodríguez-Berbel, Natalia. University of Almeria. Department of Agronomy & Center for Intensive Mediterranean Agronomy and Agri-Food Biotechnology (CIAIMBITAL); EspañaFil: Miranda, Luis. Instituto Andaluz de Investigación y Formación Agraria, Pesquera, Alimentaria y de la Producción Ecológica; EspañaFil: Santos, Berta de los. Instituto Andaluz de Investigación y Formación Agraria, Pesquera, Alimentaria y de la Producción Ecológica; EspañaFil: Camacho, María. Instituto Andaluz de Investigación y Formación Agraria, Pesquera, Alimentaria y de la Producción Ecológica; EspañaMDPI2022-05-03T17:12:21Z2022-05-03T17:12:21Z2021-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/11797https://www.mdpi.com/2311-7524/7/11/4572311-7524https://doi.org/10.3390/horticulturae7110457Horticulturae 7 (11) : 457. (November 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-23T11:17:57Zoai:localhost:20.500.12123/11797instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:17:57.57INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Potential Bacterial Antagonists for the Control of Charcoal Rot (Macrophomina phaseolina) in Strawberry
title Potential Bacterial Antagonists for the Control of Charcoal Rot (Macrophomina phaseolina) in Strawberry
spellingShingle Potential Bacterial Antagonists for the Control of Charcoal Rot (Macrophomina phaseolina) in Strawberry
Viejobueno, Josefina
Fresa
Enfermedades de las Plantas
Control de Enfermedades de Plantas
Macrophomina phaseolina
Control Biológico
Bacterias Antagonistas
Strawberries
Plant Diseases
Plant Disease Control
Biological Control
Antagonistic Bacteria
Frutilla
Charcoal Rot Disease
title_short Potential Bacterial Antagonists for the Control of Charcoal Rot (Macrophomina phaseolina) in Strawberry
title_full Potential Bacterial Antagonists for the Control of Charcoal Rot (Macrophomina phaseolina) in Strawberry
title_fullStr Potential Bacterial Antagonists for the Control of Charcoal Rot (Macrophomina phaseolina) in Strawberry
title_full_unstemmed Potential Bacterial Antagonists for the Control of Charcoal Rot (Macrophomina phaseolina) in Strawberry
title_sort Potential Bacterial Antagonists for the Control of Charcoal Rot (Macrophomina phaseolina) in Strawberry
dc.creator.none.fl_str_mv Viejobueno, Josefina
Rodríguez-Berbel, Natalia
Miranda, Luis
Santos, Berta de los
Camacho, María
author Viejobueno, Josefina
author_facet Viejobueno, Josefina
Rodríguez-Berbel, Natalia
Miranda, Luis
Santos, Berta de los
Camacho, María
author_role author
author2 Rodríguez-Berbel, Natalia
Miranda, Luis
Santos, Berta de los
Camacho, María
author2_role author
author
author
author
dc.subject.none.fl_str_mv Fresa
Enfermedades de las Plantas
Control de Enfermedades de Plantas
Macrophomina phaseolina
Control Biológico
Bacterias Antagonistas
Strawberries
Plant Diseases
Plant Disease Control
Biological Control
Antagonistic Bacteria
Frutilla
Charcoal Rot Disease
topic Fresa
Enfermedades de las Plantas
Control de Enfermedades de Plantas
Macrophomina phaseolina
Control Biológico
Bacterias Antagonistas
Strawberries
Plant Diseases
Plant Disease Control
Biological Control
Antagonistic Bacteria
Frutilla
Charcoal Rot Disease
dc.description.none.fl_txt_mv The effect of antagonistic bacteria to control Macrophomina phaseolina was evaluated under in vitro, growth chamber, greenhouse and field conditions. A total of 177 bacteria, isolated from Athrocaulon macrostachyum rhizosphere of the Lebrija marsh, were screened for their potential against M. phaseolina (causes charcoal rot in strawberry) by dual culture assay. Of these isolates, 14 most promising strains were molecularly identified by the 16S rDNA sequencing method using the EzBioCloud database. These strains were tested for in vitro hydrolytic enzymes, HCN production, and biocontrol against M. phaseolina in strawberry plants. All the 14 strains produced, at least, one hydrolytic enzymatic activity and one of them, which belongs to Brevibacterium genus (Hvs8), showed the lowest records of disease incidence (20%) and severity (0.4). With these results, greenhouse and field trials were carried out with the Hvs8 strain, compared to non-treated control. In the greenhouse assays, Hvs8 strain increased root dry mass by 30%, over the control. In the field trials, production and fruit quality were not significantly different between Hvs8 treatment and non-treated control, but plant mortality and plant mortality associated to M. phaseolina decreased by more than 24% and 65% respectively, in Hvs8 treatment. This study suggests that Brevibacterium sp. Hvs8 strain could be a candidate for controlling charcoal rot in strawberry.
EEA Famaillá
Fil: Viejobueno, Josefina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Rodríguez-Berbel, Natalia. University of Almeria. Department of Agronomy & Center for Intensive Mediterranean Agronomy and Agri-Food Biotechnology (CIAIMBITAL); España
Fil: Miranda, Luis. Instituto Andaluz de Investigación y Formación Agraria, Pesquera, Alimentaria y de la Producción Ecológica; España
Fil: Santos, Berta de los. Instituto Andaluz de Investigación y Formación Agraria, Pesquera, Alimentaria y de la Producción Ecológica; España
Fil: Camacho, María. Instituto Andaluz de Investigación y Formación Agraria, Pesquera, Alimentaria y de la Producción Ecológica; España
description The effect of antagonistic bacteria to control Macrophomina phaseolina was evaluated under in vitro, growth chamber, greenhouse and field conditions. A total of 177 bacteria, isolated from Athrocaulon macrostachyum rhizosphere of the Lebrija marsh, were screened for their potential against M. phaseolina (causes charcoal rot in strawberry) by dual culture assay. Of these isolates, 14 most promising strains were molecularly identified by the 16S rDNA sequencing method using the EzBioCloud database. These strains were tested for in vitro hydrolytic enzymes, HCN production, and biocontrol against M. phaseolina in strawberry plants. All the 14 strains produced, at least, one hydrolytic enzymatic activity and one of them, which belongs to Brevibacterium genus (Hvs8), showed the lowest records of disease incidence (20%) and severity (0.4). With these results, greenhouse and field trials were carried out with the Hvs8 strain, compared to non-treated control. In the greenhouse assays, Hvs8 strain increased root dry mass by 30%, over the control. In the field trials, production and fruit quality were not significantly different between Hvs8 treatment and non-treated control, but plant mortality and plant mortality associated to M. phaseolina decreased by more than 24% and 65% respectively, in Hvs8 treatment. This study suggests that Brevibacterium sp. Hvs8 strain could be a candidate for controlling charcoal rot in strawberry.
publishDate 2021
dc.date.none.fl_str_mv 2021-11
2022-05-03T17:12:21Z
2022-05-03T17:12:21Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/11797
https://www.mdpi.com/2311-7524/7/11/457
2311-7524
https://doi.org/10.3390/horticulturae7110457
url http://hdl.handle.net/20.500.12123/11797
https://www.mdpi.com/2311-7524/7/11/457
https://doi.org/10.3390/horticulturae7110457
identifier_str_mv 2311-7524
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Horticulturae 7 (11) : 457. (November 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.982451

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