Protection of Strawberry Plants against Charcoal Rot Disease (Macrophomina phaseolina) Induced by Azospirillum brasilense

Autores
Viejobueno, Josefina; Albornoz, Patricia Liliana; Camacho, María; Santos, Berta de los; Martinez Zamora, Martin Gustavo; Salazar, Sergio Miguel
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Some Plant Growth-Promoting Rhizobacteria (PGPR) can induce protection against pathogens, increasing plant tolerance to various diseases. This so-called biocontrol activity is replacing harmful practices in agriculture caused by the use of agrochemicals. Azospirillum brasilense is one of the PGPR already effectively used as a resistance inducer in several crops. The aim of this study was to evaluate the protective effect of PGPR A. brasilense strains isolated from strawberry and petunia plants (REC3, 2A1, 2A2, and 2E1) against the fungal pathogen Macrophomina phaseolina, which is the causal agent of the strawberry charcoal rot disease. In vitro antagonism assays and enzymatic tests on Petri dishes revealed no direct inhibition on M. phaseolina growth by any of the A. brasilense strains. However, strawberry plants treated with REC3 and 2A1 strains increased callose and lignin deposition and stomatal closure compared to untreated plants. In addition, treatments with either bacterial strains induced a defense response in strawberry plants against virulent isolates of M. phaseolina evidenced by an increased tolerance to the charcoal rot disease. These results suggest that A. brasilense REC3 and 2A1 strains can be used for the activation of innate immunity in strawberry plants as a strategy for managing charcoal rot in a sustainable and environmentally friendly way.
EEA Famaillá
Fil: Viejobueno, Josefina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Albornoz, Patricia Liliana. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales; Argentina
Fil: Albornoz, Patricia Liliana. Fundación Miguel Lillo; Argentina
Fil: Camacho, María. Instituto de Investigación y Formación Agraria y Pesquera; España
Fil: Santos, Berta de los. Instituto de Investigación y Formación Agraria y Pesquera; España
Fil: Martinez Zamora, Martin Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Martinez Zamora, Martin Gustavo. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Salazar, Sergio Miguel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Salazar, Sergio Miguel. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina
Fuente
Agronomy 11 (2) : 195 (2021)
Materia
Fresa
Protección de las Plantas
Macrophomina phaseolina
Azospirillum brasilense
Sustancias de Crecimiento Vegetal
Rhizobacteria
Strawberries
Crop Protection
Plant Growth Substances
Frutilla
Charcoal Rot Disease
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/9537
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oai_identifier_str oai:localhost:20.500.12123/9537
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spelling Protection of Strawberry Plants against Charcoal Rot Disease (Macrophomina phaseolina) Induced by Azospirillum brasilenseViejobueno, JosefinaAlbornoz, Patricia LilianaCamacho, MaríaSantos, Berta de losMartinez Zamora, Martin GustavoSalazar, Sergio MiguelFresaProtección de las PlantasMacrophomina phaseolinaAzospirillum brasilenseSustancias de Crecimiento VegetalRhizobacteriaStrawberriesCrop ProtectionPlant Growth SubstancesFrutillaCharcoal Rot DiseaseSome Plant Growth-Promoting Rhizobacteria (PGPR) can induce protection against pathogens, increasing plant tolerance to various diseases. This so-called biocontrol activity is replacing harmful practices in agriculture caused by the use of agrochemicals. Azospirillum brasilense is one of the PGPR already effectively used as a resistance inducer in several crops. The aim of this study was to evaluate the protective effect of PGPR A. brasilense strains isolated from strawberry and petunia plants (REC3, 2A1, 2A2, and 2E1) against the fungal pathogen Macrophomina phaseolina, which is the causal agent of the strawberry charcoal rot disease. In vitro antagonism assays and enzymatic tests on Petri dishes revealed no direct inhibition on M. phaseolina growth by any of the A. brasilense strains. However, strawberry plants treated with REC3 and 2A1 strains increased callose and lignin deposition and stomatal closure compared to untreated plants. In addition, treatments with either bacterial strains induced a defense response in strawberry plants against virulent isolates of M. phaseolina evidenced by an increased tolerance to the charcoal rot disease. These results suggest that A. brasilense REC3 and 2A1 strains can be used for the activation of innate immunity in strawberry plants as a strategy for managing charcoal rot in a sustainable and environmentally friendly way.EEA FamailláFil: Viejobueno, Josefina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; ArgentinaFil: Albornoz, Patricia Liliana. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales; ArgentinaFil: Albornoz, Patricia Liliana. Fundación Miguel Lillo; ArgentinaFil: Camacho, María. Instituto de Investigación y Formación Agraria y Pesquera; EspañaFil: Santos, Berta de los. Instituto de Investigación y Formación Agraria y Pesquera; EspañaFil: Martinez Zamora, Martin Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Martinez Zamora, Martin Gustavo. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Salazar, Sergio Miguel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; ArgentinaFil: Salazar, Sergio Miguel. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; ArgentinaMDPI2021-06-09T13:28:46Z2021-06-09T13:28:46Z2021-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/9537https://www.mdpi.com/2073-4395/11/2/1952073-4395https://doi.org/10.3390/agronomy11020195Agronomy 11 (2) : 195 (2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNHFA-1106073/AR./Aumento de la competitividad con sustentabilidad y equidad social de sistemas productivos de hortalizas frescas diferenciadas.info:eu-repograntAgreement/INTA/2019-PD-E4-I069-001/2019-PD-E4-I069-001/AR./Bioprospección y caracterización de microorganismos benéficos para la protección y producción vegetalinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-23T11:17:37Zoai:localhost:20.500.12123/9537instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:17:37.879INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Protection of Strawberry Plants against Charcoal Rot Disease (Macrophomina phaseolina) Induced by Azospirillum brasilense
title Protection of Strawberry Plants against Charcoal Rot Disease (Macrophomina phaseolina) Induced by Azospirillum brasilense
spellingShingle Protection of Strawberry Plants against Charcoal Rot Disease (Macrophomina phaseolina) Induced by Azospirillum brasilense
Viejobueno, Josefina
Fresa
Protección de las Plantas
Macrophomina phaseolina
Azospirillum brasilense
Sustancias de Crecimiento Vegetal
Rhizobacteria
Strawberries
Crop Protection
Plant Growth Substances
Frutilla
Charcoal Rot Disease
title_short Protection of Strawberry Plants against Charcoal Rot Disease (Macrophomina phaseolina) Induced by Azospirillum brasilense
title_full Protection of Strawberry Plants against Charcoal Rot Disease (Macrophomina phaseolina) Induced by Azospirillum brasilense
title_fullStr Protection of Strawberry Plants against Charcoal Rot Disease (Macrophomina phaseolina) Induced by Azospirillum brasilense
title_full_unstemmed Protection of Strawberry Plants against Charcoal Rot Disease (Macrophomina phaseolina) Induced by Azospirillum brasilense
title_sort Protection of Strawberry Plants against Charcoal Rot Disease (Macrophomina phaseolina) Induced by Azospirillum brasilense
dc.creator.none.fl_str_mv Viejobueno, Josefina
Albornoz, Patricia Liliana
Camacho, María
Santos, Berta de los
Martinez Zamora, Martin Gustavo
Salazar, Sergio Miguel
author Viejobueno, Josefina
author_facet Viejobueno, Josefina
Albornoz, Patricia Liliana
Camacho, María
Santos, Berta de los
Martinez Zamora, Martin Gustavo
Salazar, Sergio Miguel
author_role author
author2 Albornoz, Patricia Liliana
Camacho, María
Santos, Berta de los
Martinez Zamora, Martin Gustavo
Salazar, Sergio Miguel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Fresa
Protección de las Plantas
Macrophomina phaseolina
Azospirillum brasilense
Sustancias de Crecimiento Vegetal
Rhizobacteria
Strawberries
Crop Protection
Plant Growth Substances
Frutilla
Charcoal Rot Disease
topic Fresa
Protección de las Plantas
Macrophomina phaseolina
Azospirillum brasilense
Sustancias de Crecimiento Vegetal
Rhizobacteria
Strawberries
Crop Protection
Plant Growth Substances
Frutilla
Charcoal Rot Disease
dc.description.none.fl_txt_mv Some Plant Growth-Promoting Rhizobacteria (PGPR) can induce protection against pathogens, increasing plant tolerance to various diseases. This so-called biocontrol activity is replacing harmful practices in agriculture caused by the use of agrochemicals. Azospirillum brasilense is one of the PGPR already effectively used as a resistance inducer in several crops. The aim of this study was to evaluate the protective effect of PGPR A. brasilense strains isolated from strawberry and petunia plants (REC3, 2A1, 2A2, and 2E1) against the fungal pathogen Macrophomina phaseolina, which is the causal agent of the strawberry charcoal rot disease. In vitro antagonism assays and enzymatic tests on Petri dishes revealed no direct inhibition on M. phaseolina growth by any of the A. brasilense strains. However, strawberry plants treated with REC3 and 2A1 strains increased callose and lignin deposition and stomatal closure compared to untreated plants. In addition, treatments with either bacterial strains induced a defense response in strawberry plants against virulent isolates of M. phaseolina evidenced by an increased tolerance to the charcoal rot disease. These results suggest that A. brasilense REC3 and 2A1 strains can be used for the activation of innate immunity in strawberry plants as a strategy for managing charcoal rot in a sustainable and environmentally friendly way.
EEA Famaillá
Fil: Viejobueno, Josefina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Albornoz, Patricia Liliana. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales; Argentina
Fil: Albornoz, Patricia Liliana. Fundación Miguel Lillo; Argentina
Fil: Camacho, María. Instituto de Investigación y Formación Agraria y Pesquera; España
Fil: Santos, Berta de los. Instituto de Investigación y Formación Agraria y Pesquera; España
Fil: Martinez Zamora, Martin Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Martinez Zamora, Martin Gustavo. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Salazar, Sergio Miguel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Salazar, Sergio Miguel. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina
description Some Plant Growth-Promoting Rhizobacteria (PGPR) can induce protection against pathogens, increasing plant tolerance to various diseases. This so-called biocontrol activity is replacing harmful practices in agriculture caused by the use of agrochemicals. Azospirillum brasilense is one of the PGPR already effectively used as a resistance inducer in several crops. The aim of this study was to evaluate the protective effect of PGPR A. brasilense strains isolated from strawberry and petunia plants (REC3, 2A1, 2A2, and 2E1) against the fungal pathogen Macrophomina phaseolina, which is the causal agent of the strawberry charcoal rot disease. In vitro antagonism assays and enzymatic tests on Petri dishes revealed no direct inhibition on M. phaseolina growth by any of the A. brasilense strains. However, strawberry plants treated with REC3 and 2A1 strains increased callose and lignin deposition and stomatal closure compared to untreated plants. In addition, treatments with either bacterial strains induced a defense response in strawberry plants against virulent isolates of M. phaseolina evidenced by an increased tolerance to the charcoal rot disease. These results suggest that A. brasilense REC3 and 2A1 strains can be used for the activation of innate immunity in strawberry plants as a strategy for managing charcoal rot in a sustainable and environmentally friendly way.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-09T13:28:46Z
2021-06-09T13:28:46Z
2021-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/9537
https://www.mdpi.com/2073-4395/11/2/195
2073-4395
https://doi.org/10.3390/agronomy11020195
url http://hdl.handle.net/20.500.12123/9537
https://www.mdpi.com/2073-4395/11/2/195
https://doi.org/10.3390/agronomy11020195
identifier_str_mv 2073-4395
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/PNHFA-1106073/AR./Aumento de la competitividad con sustentabilidad y equidad social de sistemas productivos de hortalizas frescas diferenciadas.
info:eu-repograntAgreement/INTA/2019-PD-E4-I069-001/2019-PD-E4-I069-001/AR./Bioprospección y caracterización de microorganismos benéficos para la protección y producción vegetal
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Agronomy 11 (2) : 195 (2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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