Biocompatibility of core@shell particles: Cytotoxicity and genotoxicity in human osteosarcoma cells of colloidal silica spheres coated with crystalline or amorphous zirconia

Autores
Di Virgilio, Ana Laura; Arnal, Pablo Maximiliano; Maisuls, Ivan
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The cytotoxicity and genotoxicity of novel colloidal silica spheres coated with crystalline or amorphous zirconia (SiO2@ZrO2 cryst or SiO2@ZrO2 am) have been studied in a human osteosarcoma cell line (MG-63), after 24 h exposure. SiO2@ZrO2 cryst and SiO2@ZrO2 am had mean diameters of 782 ± 19 and 891 ± 34 nm, respectively. SiO2@ZrO2 cryst exposure reduced cell viability, with an increase in reactive oxygen species (ROS) and a decrease of the GSH/GSSG ratio. The comet and micronucleus (MN) assays detected DNA damage at 5 and 25 g/mL, respectively. SiO2@ZrO2 am induced genotoxic action only at 10 and 50 g/mL (comet and MN assays), along with a decrease of the GSH/GSSG ratio at 50 g/mL. Both particles were found inside the cells, forming vesicles; however, none of them entered the nucleus. Our findings show that crystallization of the shell of the amorphous ZrO2 increases both cytotoxicity and genotoxicity.
Fil: Di Virgilio, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; Argentina. Universidad Nacional de la Plata. Facultad de Cs.exactas. Departamento de Cs.biológicas. Cátedra Bioquímica Patologica; Argentina
Fil: Arnal, Pablo Maximiliano. Provincia de Buenos Aires. Gobernación. Comision de Invest.científicas. Centro de Tecnología de Recursos Minerales y Ceramica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Tecnología de Recursos Minerales y Ceramica; Argentina
Fil: Maisuls, Ivan. Universidad Nacional de la Plata. Facultad de Cs.exactas. Departamento de Cs.biológicas. Cátedra Bioquímica Patologica; Argentina. Provincia de Buenos Aires. Gobernación. Comision de Invest.científicas. Centro de Tecnología de Recursos Minerales y Ceramica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Tecnología de Recursos Minerales y Ceramica; Argentina
Materia
Core@Shell Materials
Cytotoxicity
Genotoxicity
Zirconia
A Silica
Human Osteosarcoma Cells
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/24809
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/24809
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Biocompatibility of core@shell particles: Cytotoxicity and genotoxicity in human osteosarcoma cells of colloidal silica spheres coated with crystalline or amorphous zirconiaDi Virgilio, Ana LauraArnal, Pablo MaximilianoMaisuls, IvanCore@Shell MaterialsCytotoxicityGenotoxicityZirconiaA SilicaHuman Osteosarcoma Cellshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The cytotoxicity and genotoxicity of novel colloidal silica spheres coated with crystalline or amorphous zirconia (SiO2@ZrO2 cryst or SiO2@ZrO2 am) have been studied in a human osteosarcoma cell line (MG-63), after 24 h exposure. SiO2@ZrO2 cryst and SiO2@ZrO2 am had mean diameters of 782 ± 19 and 891 ± 34 nm, respectively. SiO2@ZrO2 cryst exposure reduced cell viability, with an increase in reactive oxygen species (ROS) and a decrease of the GSH/GSSG ratio. The comet and micronucleus (MN) assays detected DNA damage at 5 and 25 g/mL, respectively. SiO2@ZrO2 am induced genotoxic action only at 10 and 50 g/mL (comet and MN assays), along with a decrease of the GSH/GSSG ratio at 50 g/mL. Both particles were found inside the cells, forming vesicles; however, none of them entered the nucleus. Our findings show that crystallization of the shell of the amorphous ZrO2 increases both cytotoxicity and genotoxicity.Fil: Di Virgilio, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; Argentina. Universidad Nacional de la Plata. Facultad de Cs.exactas. Departamento de Cs.biológicas. Cátedra Bioquímica Patologica; ArgentinaFil: Arnal, Pablo Maximiliano. Provincia de Buenos Aires. Gobernación. Comision de Invest.científicas. Centro de Tecnología de Recursos Minerales y Ceramica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Tecnología de Recursos Minerales y Ceramica; ArgentinaFil: Maisuls, Ivan. Universidad Nacional de la Plata. Facultad de Cs.exactas. Departamento de Cs.biológicas. Cátedra Bioquímica Patologica; Argentina. Provincia de Buenos Aires. Gobernación. Comision de Invest.científicas. Centro de Tecnología de Recursos Minerales y Ceramica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Tecnología de Recursos Minerales y Ceramica; ArgentinaElsevier Science2014-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24809Di Virgilio, Ana Laura; Arnal, Pablo Maximiliano; Maisuls, Ivan; Biocompatibility of core@shell particles: Cytotoxicity and genotoxicity in human osteosarcoma cells of colloidal silica spheres coated with crystalline or amorphous zirconia; Elsevier Science; Mutation Research. Genetic Toxicology And Environmental Mutagenesis; 770; 6-2014; 85-941383-5718CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1383571814001612info:eu-repo/semantics/altIdentifier/doi/10.1016/j.mrgentox.2014.05.009info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:34:38Zoai:ri.conicet.gov.ar:11336/24809instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:34:38.288CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Biocompatibility of core@shell particles: Cytotoxicity and genotoxicity in human osteosarcoma cells of colloidal silica spheres coated with crystalline or amorphous zirconia
title Biocompatibility of core@shell particles: Cytotoxicity and genotoxicity in human osteosarcoma cells of colloidal silica spheres coated with crystalline or amorphous zirconia
spellingShingle Biocompatibility of core@shell particles: Cytotoxicity and genotoxicity in human osteosarcoma cells of colloidal silica spheres coated with crystalline or amorphous zirconia
Di Virgilio, Ana Laura
Core@Shell Materials
Cytotoxicity
Genotoxicity
Zirconia
A Silica
Human Osteosarcoma Cells
title_short Biocompatibility of core@shell particles: Cytotoxicity and genotoxicity in human osteosarcoma cells of colloidal silica spheres coated with crystalline or amorphous zirconia
title_full Biocompatibility of core@shell particles: Cytotoxicity and genotoxicity in human osteosarcoma cells of colloidal silica spheres coated with crystalline or amorphous zirconia
title_fullStr Biocompatibility of core@shell particles: Cytotoxicity and genotoxicity in human osteosarcoma cells of colloidal silica spheres coated with crystalline or amorphous zirconia
title_full_unstemmed Biocompatibility of core@shell particles: Cytotoxicity and genotoxicity in human osteosarcoma cells of colloidal silica spheres coated with crystalline or amorphous zirconia
title_sort Biocompatibility of core@shell particles: Cytotoxicity and genotoxicity in human osteosarcoma cells of colloidal silica spheres coated with crystalline or amorphous zirconia
dc.creator.none.fl_str_mv Di Virgilio, Ana Laura
Arnal, Pablo Maximiliano
Maisuls, Ivan
author Di Virgilio, Ana Laura
author_facet Di Virgilio, Ana Laura
Arnal, Pablo Maximiliano
Maisuls, Ivan
author_role author
author2 Arnal, Pablo Maximiliano
Maisuls, Ivan
author2_role author
author
dc.subject.none.fl_str_mv Core@Shell Materials
Cytotoxicity
Genotoxicity
Zirconia
A Silica
Human Osteosarcoma Cells
topic Core@Shell Materials
Cytotoxicity
Genotoxicity
Zirconia
A Silica
Human Osteosarcoma Cells
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The cytotoxicity and genotoxicity of novel colloidal silica spheres coated with crystalline or amorphous zirconia (SiO2@ZrO2 cryst or SiO2@ZrO2 am) have been studied in a human osteosarcoma cell line (MG-63), after 24 h exposure. SiO2@ZrO2 cryst and SiO2@ZrO2 am had mean diameters of 782 ± 19 and 891 ± 34 nm, respectively. SiO2@ZrO2 cryst exposure reduced cell viability, with an increase in reactive oxygen species (ROS) and a decrease of the GSH/GSSG ratio. The comet and micronucleus (MN) assays detected DNA damage at 5 and 25 g/mL, respectively. SiO2@ZrO2 am induced genotoxic action only at 10 and 50 g/mL (comet and MN assays), along with a decrease of the GSH/GSSG ratio at 50 g/mL. Both particles were found inside the cells, forming vesicles; however, none of them entered the nucleus. Our findings show that crystallization of the shell of the amorphous ZrO2 increases both cytotoxicity and genotoxicity.
Fil: Di Virgilio, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; Argentina. Universidad Nacional de la Plata. Facultad de Cs.exactas. Departamento de Cs.biológicas. Cátedra Bioquímica Patologica; Argentina
Fil: Arnal, Pablo Maximiliano. Provincia de Buenos Aires. Gobernación. Comision de Invest.científicas. Centro de Tecnología de Recursos Minerales y Ceramica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Tecnología de Recursos Minerales y Ceramica; Argentina
Fil: Maisuls, Ivan. Universidad Nacional de la Plata. Facultad de Cs.exactas. Departamento de Cs.biológicas. Cátedra Bioquímica Patologica; Argentina. Provincia de Buenos Aires. Gobernación. Comision de Invest.científicas. Centro de Tecnología de Recursos Minerales y Ceramica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Tecnología de Recursos Minerales y Ceramica; Argentina
description The cytotoxicity and genotoxicity of novel colloidal silica spheres coated with crystalline or amorphous zirconia (SiO2@ZrO2 cryst or SiO2@ZrO2 am) have been studied in a human osteosarcoma cell line (MG-63), after 24 h exposure. SiO2@ZrO2 cryst and SiO2@ZrO2 am had mean diameters of 782 ± 19 and 891 ± 34 nm, respectively. SiO2@ZrO2 cryst exposure reduced cell viability, with an increase in reactive oxygen species (ROS) and a decrease of the GSH/GSSG ratio. The comet and micronucleus (MN) assays detected DNA damage at 5 and 25 g/mL, respectively. SiO2@ZrO2 am induced genotoxic action only at 10 and 50 g/mL (comet and MN assays), along with a decrease of the GSH/GSSG ratio at 50 g/mL. Both particles were found inside the cells, forming vesicles; however, none of them entered the nucleus. Our findings show that crystallization of the shell of the amorphous ZrO2 increases both cytotoxicity and genotoxicity.
publishDate 2014
dc.date.none.fl_str_mv 2014-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/24809
Di Virgilio, Ana Laura; Arnal, Pablo Maximiliano; Maisuls, Ivan; Biocompatibility of core@shell particles: Cytotoxicity and genotoxicity in human osteosarcoma cells of colloidal silica spheres coated with crystalline or amorphous zirconia; Elsevier Science; Mutation Research. Genetic Toxicology And Environmental Mutagenesis; 770; 6-2014; 85-94
1383-5718
CONICET Digital
CONICET
url http://hdl.handle.net/11336/24809
identifier_str_mv Di Virgilio, Ana Laura; Arnal, Pablo Maximiliano; Maisuls, Ivan; Biocompatibility of core@shell particles: Cytotoxicity and genotoxicity in human osteosarcoma cells of colloidal silica spheres coated with crystalline or amorphous zirconia; Elsevier Science; Mutation Research. Genetic Toxicology And Environmental Mutagenesis; 770; 6-2014; 85-94
1383-5718
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1383571814001612
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.mrgentox.2014.05.009
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613072408281088
score 13.070432

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