Synthesis of ultraluminescent gold core-shell nanoparticles as nanoimaging platforms for biosensing applications based on metal-enhanced fluorescence

Autores
Gontero, Daniela Ivana; Veglia, Alicia Viviana; Bracamonte, Angel Guillermo; Boudreau, Denis
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Core-shell nanoparticles are versatile nanostructures that can be used as luminescent biosensing platforms in many nanotechnological developments. Ultraluminescent fluorescent gold core-shell nanoparticles based on Metal-Enhanced Fluorescence (MEF) were synthesized. The nanoparticles obtained were formed by 40.0 nm cores and variable silica spacer lengths. Silica spacer lengths from 6.0 to 25.0 nm were obtained. The plasmon maximal wavelength of the core-shell nanoparticles was shifted to a longer wavelength from a gold nanoparticle plasmon centered at 537.0 nm to 545.0 nm and 548 nm from 6.0 nm to 20.0 nm spacer length, respectively. The effect of the gold core on emission was evaluated by determination of Metal Enhanced Fluorescence enhancement factors (MEFEF), applying the sodium cyanide method for core leaching. We observed maximal MEFEF = 8.1 and 7.2 for 6.0 and 14.0 nm, respectively, and a significant decrease at longer silica spacer lengths. From nanoimaging by confocal fluorescence microscopy it was possible to detect ultraluminescent gold core-shell nanoparticle aggregates and obtain an MEFEF that can rise to 40. These parameters and properties were discussed from the point of view of fluorescent platform applications. Moreover in order to show the potential application of these nanoparticles in biodetection and nanomedicine, Escherichia coli bacteria were labelled with ultraluminescent nanoparticles. Bright and clear bacteria images were obtained by laser fluorescence microscopy. Based on these results, future applications for individual bacterial detection will be developed.
Fil: Gontero, Daniela Ivana. Clínica de la Familia II. Laboratorio de Análisis Clínicos y Bacteriológicos; Argentina
Fil: Veglia, Alicia Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Bracamonte, Angel Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Boudreau, Denis. Universite Laval; Francia
Materia
NANOPARTICLES
FLUORESCENCE
BACTERIA
DETECTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/62314
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/62314
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Synthesis of ultraluminescent gold core-shell nanoparticles as nanoimaging platforms for biosensing applications based on metal-enhanced fluorescenceGontero, Daniela IvanaVeglia, Alicia VivianaBracamonte, Angel GuillermoBoudreau, DenisNANOPARTICLESFLUORESCENCEBACTERIADETECTIONhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Core-shell nanoparticles are versatile nanostructures that can be used as luminescent biosensing platforms in many nanotechnological developments. Ultraluminescent fluorescent gold core-shell nanoparticles based on Metal-Enhanced Fluorescence (MEF) were synthesized. The nanoparticles obtained were formed by 40.0 nm cores and variable silica spacer lengths. Silica spacer lengths from 6.0 to 25.0 nm were obtained. The plasmon maximal wavelength of the core-shell nanoparticles was shifted to a longer wavelength from a gold nanoparticle plasmon centered at 537.0 nm to 545.0 nm and 548 nm from 6.0 nm to 20.0 nm spacer length, respectively. The effect of the gold core on emission was evaluated by determination of Metal Enhanced Fluorescence enhancement factors (MEFEF), applying the sodium cyanide method for core leaching. We observed maximal MEFEF = 8.1 and 7.2 for 6.0 and 14.0 nm, respectively, and a significant decrease at longer silica spacer lengths. From nanoimaging by confocal fluorescence microscopy it was possible to detect ultraluminescent gold core-shell nanoparticle aggregates and obtain an MEFEF that can rise to 40. These parameters and properties were discussed from the point of view of fluorescent platform applications. Moreover in order to show the potential application of these nanoparticles in biodetection and nanomedicine, Escherichia coli bacteria were labelled with ultraluminescent nanoparticles. Bright and clear bacteria images were obtained by laser fluorescence microscopy. Based on these results, future applications for individual bacterial detection will be developed.Fil: Gontero, Daniela Ivana. Clínica de la Familia II. Laboratorio de Análisis Clínicos y Bacteriológicos; ArgentinaFil: Veglia, Alicia Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Bracamonte, Angel Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Boudreau, Denis. Universite Laval; FranciaRoyal Society of Chemistry2017-02-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/62314Gontero, Daniela Ivana; Veglia, Alicia Viviana; Bracamonte, Angel Guillermo; Boudreau, Denis; Synthesis of ultraluminescent gold core-shell nanoparticles as nanoimaging platforms for biosensing applications based on metal-enhanced fluorescence; Royal Society of Chemistry; RSC Advances; 7; 17; 6-2-2017; 10252-102582046-20692046-2069CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pubs.rsc.org/en/Content/ArticleLanding/2017/RA/C6RA27649Kinfo:eu-repo/semantics/altIdentifier/doi/10.1039/c6ra27649kinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:52:48Zoai:ri.conicet.gov.ar:11336/62314instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:49.106CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Synthesis of ultraluminescent gold core-shell nanoparticles as nanoimaging platforms for biosensing applications based on metal-enhanced fluorescence
title Synthesis of ultraluminescent gold core-shell nanoparticles as nanoimaging platforms for biosensing applications based on metal-enhanced fluorescence
spellingShingle Synthesis of ultraluminescent gold core-shell nanoparticles as nanoimaging platforms for biosensing applications based on metal-enhanced fluorescence
Gontero, Daniela Ivana
NANOPARTICLES
FLUORESCENCE
BACTERIA
DETECTION
title_short Synthesis of ultraluminescent gold core-shell nanoparticles as nanoimaging platforms for biosensing applications based on metal-enhanced fluorescence
title_full Synthesis of ultraluminescent gold core-shell nanoparticles as nanoimaging platforms for biosensing applications based on metal-enhanced fluorescence
title_fullStr Synthesis of ultraluminescent gold core-shell nanoparticles as nanoimaging platforms for biosensing applications based on metal-enhanced fluorescence
title_full_unstemmed Synthesis of ultraluminescent gold core-shell nanoparticles as nanoimaging platforms for biosensing applications based on metal-enhanced fluorescence
title_sort Synthesis of ultraluminescent gold core-shell nanoparticles as nanoimaging platforms for biosensing applications based on metal-enhanced fluorescence
dc.creator.none.fl_str_mv Gontero, Daniela Ivana
Veglia, Alicia Viviana
Bracamonte, Angel Guillermo
Boudreau, Denis
author Gontero, Daniela Ivana
author_facet Gontero, Daniela Ivana
Veglia, Alicia Viviana
Bracamonte, Angel Guillermo
Boudreau, Denis
author_role author
author2 Veglia, Alicia Viviana
Bracamonte, Angel Guillermo
Boudreau, Denis
author2_role author
author
author
dc.subject.none.fl_str_mv NANOPARTICLES
FLUORESCENCE
BACTERIA
DETECTION
topic NANOPARTICLES
FLUORESCENCE
BACTERIA
DETECTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Core-shell nanoparticles are versatile nanostructures that can be used as luminescent biosensing platforms in many nanotechnological developments. Ultraluminescent fluorescent gold core-shell nanoparticles based on Metal-Enhanced Fluorescence (MEF) were synthesized. The nanoparticles obtained were formed by 40.0 nm cores and variable silica spacer lengths. Silica spacer lengths from 6.0 to 25.0 nm were obtained. The plasmon maximal wavelength of the core-shell nanoparticles was shifted to a longer wavelength from a gold nanoparticle plasmon centered at 537.0 nm to 545.0 nm and 548 nm from 6.0 nm to 20.0 nm spacer length, respectively. The effect of the gold core on emission was evaluated by determination of Metal Enhanced Fluorescence enhancement factors (MEFEF), applying the sodium cyanide method for core leaching. We observed maximal MEFEF = 8.1 and 7.2 for 6.0 and 14.0 nm, respectively, and a significant decrease at longer silica spacer lengths. From nanoimaging by confocal fluorescence microscopy it was possible to detect ultraluminescent gold core-shell nanoparticle aggregates and obtain an MEFEF that can rise to 40. These parameters and properties were discussed from the point of view of fluorescent platform applications. Moreover in order to show the potential application of these nanoparticles in biodetection and nanomedicine, Escherichia coli bacteria were labelled with ultraluminescent nanoparticles. Bright and clear bacteria images were obtained by laser fluorescence microscopy. Based on these results, future applications for individual bacterial detection will be developed.
Fil: Gontero, Daniela Ivana. Clínica de la Familia II. Laboratorio de Análisis Clínicos y Bacteriológicos; Argentina
Fil: Veglia, Alicia Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Bracamonte, Angel Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Boudreau, Denis. Universite Laval; Francia
description Core-shell nanoparticles are versatile nanostructures that can be used as luminescent biosensing platforms in many nanotechnological developments. Ultraluminescent fluorescent gold core-shell nanoparticles based on Metal-Enhanced Fluorescence (MEF) were synthesized. The nanoparticles obtained were formed by 40.0 nm cores and variable silica spacer lengths. Silica spacer lengths from 6.0 to 25.0 nm were obtained. The plasmon maximal wavelength of the core-shell nanoparticles was shifted to a longer wavelength from a gold nanoparticle plasmon centered at 537.0 nm to 545.0 nm and 548 nm from 6.0 nm to 20.0 nm spacer length, respectively. The effect of the gold core on emission was evaluated by determination of Metal Enhanced Fluorescence enhancement factors (MEFEF), applying the sodium cyanide method for core leaching. We observed maximal MEFEF = 8.1 and 7.2 for 6.0 and 14.0 nm, respectively, and a significant decrease at longer silica spacer lengths. From nanoimaging by confocal fluorescence microscopy it was possible to detect ultraluminescent gold core-shell nanoparticle aggregates and obtain an MEFEF that can rise to 40. These parameters and properties were discussed from the point of view of fluorescent platform applications. Moreover in order to show the potential application of these nanoparticles in biodetection and nanomedicine, Escherichia coli bacteria were labelled with ultraluminescent nanoparticles. Bright and clear bacteria images were obtained by laser fluorescence microscopy. Based on these results, future applications for individual bacterial detection will be developed.
publishDate 2017
dc.date.none.fl_str_mv 2017-02-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/62314
Gontero, Daniela Ivana; Veglia, Alicia Viviana; Bracamonte, Angel Guillermo; Boudreau, Denis; Synthesis of ultraluminescent gold core-shell nanoparticles as nanoimaging platforms for biosensing applications based on metal-enhanced fluorescence; Royal Society of Chemistry; RSC Advances; 7; 17; 6-2-2017; 10252-10258
2046-2069
2046-2069
CONICET Digital
CONICET
url http://hdl.handle.net/11336/62314
identifier_str_mv Gontero, Daniela Ivana; Veglia, Alicia Viviana; Bracamonte, Angel Guillermo; Boudreau, Denis; Synthesis of ultraluminescent gold core-shell nanoparticles as nanoimaging platforms for biosensing applications based on metal-enhanced fluorescence; Royal Society of Chemistry; RSC Advances; 7; 17; 6-2-2017; 10252-10258
2046-2069
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://pubs.rsc.org/en/Content/ArticleLanding/2017/RA/C6RA27649K
info:eu-repo/semantics/altIdentifier/doi/10.1039/c6ra27649k
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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