Screening and quantification of the enzymatic deglycosylation of the plant flavonoid rutin by UV-visible spectrometry

Autores
Weiz, Gisela; Breccia, Javier Dario; Mazzaferro, Laura
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Rutin is a plant flavonoid constituted by the flavonol quercetin 3-O-linked to the disaccharide rutinose (quercetin 3-O-(6-O-α-L-rhamnopyranosyl-β-D-glucopyranoside)). The enzymatic deglycosylation of rutin is usually assessed by means of the standard method for flavonoid quantification (high performance liquid chromatography, HPLC). In this work, we have developed a spectrophotometric method for the quantification of the released quercetin. After the enzymatic reaction, quercetin is extracted with ethyl acetate, and subsequently oxidized under basic conditions. The absorbance of quercetin autooxidation products at 320 nm was correlated with the quercetin concentration by linear regression (molar extinction coefficient 26.5 (± 0.3) × 103 M-1 cm-1). The convenience of this method relies on the enzymatic activity quantification using the natural substrate by UV-visible spectrometry. Moreover, the simplicity and speed of analysis allows its application for the assay of a large number of samples.
Fil: Weiz, Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; Argentina
Fil: Breccia, Javier Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; Argentina
Fil: Mazzaferro, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; Argentina
Materia
Glucosidase
Rhamnosidase
Rutin Degrading Enzymes
Naringinase
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/41132
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Screening and quantification of the enzymatic deglycosylation of the plant flavonoid rutin by UV-visible spectrometryWeiz, GiselaBreccia, Javier DarioMazzaferro, LauraGlucosidaseRhamnosidaseRutin Degrading EnzymesNaringinasehttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Rutin is a plant flavonoid constituted by the flavonol quercetin 3-O-linked to the disaccharide rutinose (quercetin 3-O-(6-O-α-L-rhamnopyranosyl-β-D-glucopyranoside)). The enzymatic deglycosylation of rutin is usually assessed by means of the standard method for flavonoid quantification (high performance liquid chromatography, HPLC). In this work, we have developed a spectrophotometric method for the quantification of the released quercetin. After the enzymatic reaction, quercetin is extracted with ethyl acetate, and subsequently oxidized under basic conditions. The absorbance of quercetin autooxidation products at 320 nm was correlated with the quercetin concentration by linear regression (molar extinction coefficient 26.5 (± 0.3) × 103 M-1 cm-1). The convenience of this method relies on the enzymatic activity quantification using the natural substrate by UV-visible spectrometry. Moreover, the simplicity and speed of analysis allows its application for the assay of a large number of samples.Fil: Weiz, Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; ArgentinaFil: Breccia, Javier Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; ArgentinaFil: Mazzaferro, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; ArgentinaElsevier2017-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/41132Weiz, Gisela; Breccia, Javier Dario; Mazzaferro, Laura; Screening and quantification of the enzymatic deglycosylation of the plant flavonoid rutin by UV-visible spectrometry; Elsevier; Food Chemistry; 229; 2-2017; 44-490308-8146CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodchem.2017.02.029info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0308814617302170info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:37:35Zoai:ri.conicet.gov.ar:11336/41132instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:37:35.929CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Screening and quantification of the enzymatic deglycosylation of the plant flavonoid rutin by UV-visible spectrometry
title Screening and quantification of the enzymatic deglycosylation of the plant flavonoid rutin by UV-visible spectrometry
spellingShingle Screening and quantification of the enzymatic deglycosylation of the plant flavonoid rutin by UV-visible spectrometry
Weiz, Gisela
Glucosidase
Rhamnosidase
Rutin Degrading Enzymes
Naringinase
title_short Screening and quantification of the enzymatic deglycosylation of the plant flavonoid rutin by UV-visible spectrometry
title_full Screening and quantification of the enzymatic deglycosylation of the plant flavonoid rutin by UV-visible spectrometry
title_fullStr Screening and quantification of the enzymatic deglycosylation of the plant flavonoid rutin by UV-visible spectrometry
title_full_unstemmed Screening and quantification of the enzymatic deglycosylation of the plant flavonoid rutin by UV-visible spectrometry
title_sort Screening and quantification of the enzymatic deglycosylation of the plant flavonoid rutin by UV-visible spectrometry
dc.creator.none.fl_str_mv Weiz, Gisela
Breccia, Javier Dario
Mazzaferro, Laura
author Weiz, Gisela
author_facet Weiz, Gisela
Breccia, Javier Dario
Mazzaferro, Laura
author_role author
author2 Breccia, Javier Dario
Mazzaferro, Laura
author2_role author
author
dc.subject.none.fl_str_mv Glucosidase
Rhamnosidase
Rutin Degrading Enzymes
Naringinase
topic Glucosidase
Rhamnosidase
Rutin Degrading Enzymes
Naringinase
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Rutin is a plant flavonoid constituted by the flavonol quercetin 3-O-linked to the disaccharide rutinose (quercetin 3-O-(6-O-α-L-rhamnopyranosyl-β-D-glucopyranoside)). The enzymatic deglycosylation of rutin is usually assessed by means of the standard method for flavonoid quantification (high performance liquid chromatography, HPLC). In this work, we have developed a spectrophotometric method for the quantification of the released quercetin. After the enzymatic reaction, quercetin is extracted with ethyl acetate, and subsequently oxidized under basic conditions. The absorbance of quercetin autooxidation products at 320 nm was correlated with the quercetin concentration by linear regression (molar extinction coefficient 26.5 (± 0.3) × 103 M-1 cm-1). The convenience of this method relies on the enzymatic activity quantification using the natural substrate by UV-visible spectrometry. Moreover, the simplicity and speed of analysis allows its application for the assay of a large number of samples.
Fil: Weiz, Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; Argentina
Fil: Breccia, Javier Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; Argentina
Fil: Mazzaferro, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; Argentina
description Rutin is a plant flavonoid constituted by the flavonol quercetin 3-O-linked to the disaccharide rutinose (quercetin 3-O-(6-O-α-L-rhamnopyranosyl-β-D-glucopyranoside)). The enzymatic deglycosylation of rutin is usually assessed by means of the standard method for flavonoid quantification (high performance liquid chromatography, HPLC). In this work, we have developed a spectrophotometric method for the quantification of the released quercetin. After the enzymatic reaction, quercetin is extracted with ethyl acetate, and subsequently oxidized under basic conditions. The absorbance of quercetin autooxidation products at 320 nm was correlated with the quercetin concentration by linear regression (molar extinction coefficient 26.5 (± 0.3) × 103 M-1 cm-1). The convenience of this method relies on the enzymatic activity quantification using the natural substrate by UV-visible spectrometry. Moreover, the simplicity and speed of analysis allows its application for the assay of a large number of samples.
publishDate 2017
dc.date.none.fl_str_mv 2017-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/41132
Weiz, Gisela; Breccia, Javier Dario; Mazzaferro, Laura; Screening and quantification of the enzymatic deglycosylation of the plant flavonoid rutin by UV-visible spectrometry; Elsevier; Food Chemistry; 229; 2-2017; 44-49
0308-8146
CONICET Digital
CONICET
url http://hdl.handle.net/11336/41132
identifier_str_mv Weiz, Gisela; Breccia, Javier Dario; Mazzaferro, Laura; Screening and quantification of the enzymatic deglycosylation of the plant flavonoid rutin by UV-visible spectrometry; Elsevier; Food Chemistry; 229; 2-2017; 44-49
0308-8146
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodchem.2017.02.029
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0308814617302170
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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