α -L-Rhamnosidase and β -D-glucosidase activities in fungal strains isolated from alkaline soils and their potential in naringin hydrolysis

Autores
Eliades, Lorena Alejandra; Rojas, Natalia Lorena; Cabello, Marta Noemí; Voget, Claudio Enrique; Saparrat, Mario Carlos Nazareno
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
α -L-Rhamnosidases (EC 3.2.1.40) and β -D-glucosidases (EC 3.2.1.21) obtained from several microbial sources are potential catalysts in food, beverage, and pharmaceutical industries. However, the enzyme preparations currently used have limitations related to the stability and activity of the enzyme as well to their reuse. A microtiter screening was carried out in 55 fungal strains isolated from alkaline soils, to obtain active α -L-rhamnosidases and β -D-glucosidases at pH 9.0. While α -L-rhamnosidase activity was detected in 45% of the strains tested, β -D-glucosidase activity was found only in 27%. Based on the fungal ability to produce α -L-rhamnosidase activity, cultures were supplemented with naringin to study the activities of the enzymes and the potential of the fungal strains on naringin hydrolysis. About 70% of the fungal strains tested increased the activities of both enzymes in the naringin-supplemented cultures as compared to non-supplemented ones. This effect was higher in Acrostalagmus luteo-albus LPSC 427 (15.3 fold) for α -L-rhamnosidase activity and Metarrhizium anisopliae LPSC 996 (51.1 fold) for β -D-glucosidase activity. All the enzyme preparations tested hydrolyzed naringin at pH 9.0, being that obtained from Acremonium murorun LPSC 927 cultures the one which showed highest hydrolysis. Here, different fungal species are reported for the first time for their ability to produce α -L-rhamnosidase and β -D-glucosidase activity at alkaline pH.
Fil: Eliades, Lorena Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rojas, Natalia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Cabello, Marta Noemí. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Fil: Voget, Claudio Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Saparrat, Mario Carlos Nazareno. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
Materia
Α -L-RHAMNOSIDASE
Β -D-GLUCOSIDASE
ALKALINE ENZYMES
FUNGI
NARINGIN HYDROLYSIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/184062
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/184062
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling α -L-Rhamnosidase and β -D-glucosidase activities in fungal strains isolated from alkaline soils and their potential in naringin hydrolysisEliades, Lorena AlejandraRojas, Natalia LorenaCabello, Marta NoemíVoget, Claudio EnriqueSaparrat, Mario Carlos NazarenoΑ -L-RHAMNOSIDASEΒ -D-GLUCOSIDASEALKALINE ENZYMESFUNGINARINGIN HYDROLYSIShttps://purl.org/becyt/ford/1.7https://purl.org/becyt/ford/1α -L-Rhamnosidases (EC 3.2.1.40) and β -D-glucosidases (EC 3.2.1.21) obtained from several microbial sources are potential catalysts in food, beverage, and pharmaceutical industries. However, the enzyme preparations currently used have limitations related to the stability and activity of the enzyme as well to their reuse. A microtiter screening was carried out in 55 fungal strains isolated from alkaline soils, to obtain active α -L-rhamnosidases and β -D-glucosidases at pH 9.0. While α -L-rhamnosidase activity was detected in 45% of the strains tested, β -D-glucosidase activity was found only in 27%. Based on the fungal ability to produce α -L-rhamnosidase activity, cultures were supplemented with naringin to study the activities of the enzymes and the potential of the fungal strains on naringin hydrolysis. About 70% of the fungal strains tested increased the activities of both enzymes in the naringin-supplemented cultures as compared to non-supplemented ones. This effect was higher in Acrostalagmus luteo-albus LPSC 427 (15.3 fold) for α -L-rhamnosidase activity and Metarrhizium anisopliae LPSC 996 (51.1 fold) for β -D-glucosidase activity. All the enzyme preparations tested hydrolyzed naringin at pH 9.0, being that obtained from Acremonium murorun LPSC 927 cultures the one which showed highest hydrolysis. Here, different fungal species are reported for the first time for their ability to produce α -L-rhamnosidase and β -D-glucosidase activity at alkaline pH.Fil: Eliades, Lorena Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rojas, Natalia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Cabello, Marta Noemí. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaFil: Voget, Claudio Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Saparrat, Mario Carlos Nazareno. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; ArgentinaWiley VCH Verlag2011-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/184062Eliades, Lorena Alejandra; Rojas, Natalia Lorena; Cabello, Marta Noemí; Voget, Claudio Enrique; Saparrat, Mario Carlos Nazareno; α -L-Rhamnosidase and β -D-glucosidase activities in fungal strains isolated from alkaline soils and their potential in naringin hydrolysis; Wiley VCH Verlag; Journal of Basic Microbiology; 51; 6; 12-2011; 659-6650233-111XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/jobm.201100163info:eu-repo/semantics/altIdentifier/doi/10.1002/jobm.201100163info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:14:35Zoai:ri.conicet.gov.ar:11336/184062instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:14:35.802CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv α -L-Rhamnosidase and β -D-glucosidase activities in fungal strains isolated from alkaline soils and their potential in naringin hydrolysis
title α -L-Rhamnosidase and β -D-glucosidase activities in fungal strains isolated from alkaline soils and their potential in naringin hydrolysis
spellingShingle α -L-Rhamnosidase and β -D-glucosidase activities in fungal strains isolated from alkaline soils and their potential in naringin hydrolysis
Eliades, Lorena Alejandra
Α -L-RHAMNOSIDASE
Β -D-GLUCOSIDASE
ALKALINE ENZYMES
FUNGI
NARINGIN HYDROLYSIS
title_short α -L-Rhamnosidase and β -D-glucosidase activities in fungal strains isolated from alkaline soils and their potential in naringin hydrolysis
title_full α -L-Rhamnosidase and β -D-glucosidase activities in fungal strains isolated from alkaline soils and their potential in naringin hydrolysis
title_fullStr α -L-Rhamnosidase and β -D-glucosidase activities in fungal strains isolated from alkaline soils and their potential in naringin hydrolysis
title_full_unstemmed α -L-Rhamnosidase and β -D-glucosidase activities in fungal strains isolated from alkaline soils and their potential in naringin hydrolysis
title_sort α -L-Rhamnosidase and β -D-glucosidase activities in fungal strains isolated from alkaline soils and their potential in naringin hydrolysis
dc.creator.none.fl_str_mv Eliades, Lorena Alejandra
Rojas, Natalia Lorena
Cabello, Marta Noemí
Voget, Claudio Enrique
Saparrat, Mario Carlos Nazareno
author Eliades, Lorena Alejandra
author_facet Eliades, Lorena Alejandra
Rojas, Natalia Lorena
Cabello, Marta Noemí
Voget, Claudio Enrique
Saparrat, Mario Carlos Nazareno
author_role author
author2 Rojas, Natalia Lorena
Cabello, Marta Noemí
Voget, Claudio Enrique
Saparrat, Mario Carlos Nazareno
author2_role author
author
author
author
dc.subject.none.fl_str_mv Α -L-RHAMNOSIDASE
Β -D-GLUCOSIDASE
ALKALINE ENZYMES
FUNGI
NARINGIN HYDROLYSIS
topic Α -L-RHAMNOSIDASE
Β -D-GLUCOSIDASE
ALKALINE ENZYMES
FUNGI
NARINGIN HYDROLYSIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.7
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv α -L-Rhamnosidases (EC 3.2.1.40) and β -D-glucosidases (EC 3.2.1.21) obtained from several microbial sources are potential catalysts in food, beverage, and pharmaceutical industries. However, the enzyme preparations currently used have limitations related to the stability and activity of the enzyme as well to their reuse. A microtiter screening was carried out in 55 fungal strains isolated from alkaline soils, to obtain active α -L-rhamnosidases and β -D-glucosidases at pH 9.0. While α -L-rhamnosidase activity was detected in 45% of the strains tested, β -D-glucosidase activity was found only in 27%. Based on the fungal ability to produce α -L-rhamnosidase activity, cultures were supplemented with naringin to study the activities of the enzymes and the potential of the fungal strains on naringin hydrolysis. About 70% of the fungal strains tested increased the activities of both enzymes in the naringin-supplemented cultures as compared to non-supplemented ones. This effect was higher in Acrostalagmus luteo-albus LPSC 427 (15.3 fold) for α -L-rhamnosidase activity and Metarrhizium anisopliae LPSC 996 (51.1 fold) for β -D-glucosidase activity. All the enzyme preparations tested hydrolyzed naringin at pH 9.0, being that obtained from Acremonium murorun LPSC 927 cultures the one which showed highest hydrolysis. Here, different fungal species are reported for the first time for their ability to produce α -L-rhamnosidase and β -D-glucosidase activity at alkaline pH.
Fil: Eliades, Lorena Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rojas, Natalia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Cabello, Marta Noemí. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Fil: Voget, Claudio Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Saparrat, Mario Carlos Nazareno. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
description α -L-Rhamnosidases (EC 3.2.1.40) and β -D-glucosidases (EC 3.2.1.21) obtained from several microbial sources are potential catalysts in food, beverage, and pharmaceutical industries. However, the enzyme preparations currently used have limitations related to the stability and activity of the enzyme as well to their reuse. A microtiter screening was carried out in 55 fungal strains isolated from alkaline soils, to obtain active α -L-rhamnosidases and β -D-glucosidases at pH 9.0. While α -L-rhamnosidase activity was detected in 45% of the strains tested, β -D-glucosidase activity was found only in 27%. Based on the fungal ability to produce α -L-rhamnosidase activity, cultures were supplemented with naringin to study the activities of the enzymes and the potential of the fungal strains on naringin hydrolysis. About 70% of the fungal strains tested increased the activities of both enzymes in the naringin-supplemented cultures as compared to non-supplemented ones. This effect was higher in Acrostalagmus luteo-albus LPSC 427 (15.3 fold) for α -L-rhamnosidase activity and Metarrhizium anisopliae LPSC 996 (51.1 fold) for β -D-glucosidase activity. All the enzyme preparations tested hydrolyzed naringin at pH 9.0, being that obtained from Acremonium murorun LPSC 927 cultures the one which showed highest hydrolysis. Here, different fungal species are reported for the first time for their ability to produce α -L-rhamnosidase and β -D-glucosidase activity at alkaline pH.
publishDate 2011
dc.date.none.fl_str_mv 2011-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/184062
Eliades, Lorena Alejandra; Rojas, Natalia Lorena; Cabello, Marta Noemí; Voget, Claudio Enrique; Saparrat, Mario Carlos Nazareno; α -L-Rhamnosidase and β -D-glucosidase activities in fungal strains isolated from alkaline soils and their potential in naringin hydrolysis; Wiley VCH Verlag; Journal of Basic Microbiology; 51; 6; 12-2011; 659-665
0233-111X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/184062
identifier_str_mv Eliades, Lorena Alejandra; Rojas, Natalia Lorena; Cabello, Marta Noemí; Voget, Claudio Enrique; Saparrat, Mario Carlos Nazareno; α -L-Rhamnosidase and β -D-glucosidase activities in fungal strains isolated from alkaline soils and their potential in naringin hydrolysis; Wiley VCH Verlag; Journal of Basic Microbiology; 51; 6; 12-2011; 659-665
0233-111X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/jobm.201100163
info:eu-repo/semantics/altIdentifier/doi/10.1002/jobm.201100163
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley VCH Verlag
publisher.none.fl_str_mv Wiley VCH Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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