A Fluorometric Enzymatic Assay for Quantification of Steryl Glucosides in Biodiesel

Autores
Aguirre, Andres; Cabruja, Matias Ezequiel; Cabrera, Rodolfo; Eberhardt, Maria Florencia; Peirú, Salvador; Menzella, Hugo Gabriel; Rasia, Rodolfo Maximiliano
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Steryl glucosides (SG) are common contaminants in biodiesel that form precipitates, which form and cause problems due to fouling during transport and storage. Therefore, their quantification is necessary to assess the quality of this fuel. The methods currently available for SG analysis require expensive instrumentation, need a previous concentration step by solid-phase extraction (SPE) or are of limited use for the quantitative assessment. We developed an enzymatic method for SG quantification in biodiesel samples based on the hydrolysis of the glucoside catalyzed by a broadly specific beta glucosidase and the subsequent determination of the glucose released by the reaction. The method is non-expensive, sensitive and was adapted to 96-well format fluorescence plate reader, making it useful for the parallel assay of multiple samples. The enzymatic assay presented here represent a valuable tool for both quality control and the development of improved biodiesel production and purification procedures.
Fil: Aguirre, Andres. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Tecnologia; Argentina. Keclon S.A,; Argentina
Fil: Cabruja, Matias Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Cabrera, Rodolfo. Unitec Bio S.A; Argentina
Fil: Eberhardt, Maria Florencia. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Tecnologia; Argentina. Keclon S.A,; Argentina
Fil: Peirú, Salvador. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Tecnologia; Argentina. Keclon S.A,; Argentina
Fil: Menzella, Hugo Gabriel. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Tecnologia; Argentina. Keclon S.A; Argentina
Fil: Rasia, Rodolfo Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Materia
Biodiesel
Steryl Glucosides
Fluorescence
Nadph
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/8625
Acceder
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network_name_str CONICET Digital (CONICET)
spelling A Fluorometric Enzymatic Assay for Quantification of Steryl Glucosides in BiodieselAguirre, AndresCabruja, Matias EzequielCabrera, RodolfoEberhardt, Maria FlorenciaPeirú, SalvadorMenzella, Hugo GabrielRasia, Rodolfo MaximilianoBiodieselSteryl GlucosidesFluorescenceNadphhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Steryl glucosides (SG) are common contaminants in biodiesel that form precipitates, which form and cause problems due to fouling during transport and storage. Therefore, their quantification is necessary to assess the quality of this fuel. The methods currently available for SG analysis require expensive instrumentation, need a previous concentration step by solid-phase extraction (SPE) or are of limited use for the quantitative assessment. We developed an enzymatic method for SG quantification in biodiesel samples based on the hydrolysis of the glucoside catalyzed by a broadly specific beta glucosidase and the subsequent determination of the glucose released by the reaction. The method is non-expensive, sensitive and was adapted to 96-well format fluorescence plate reader, making it useful for the parallel assay of multiple samples. The enzymatic assay presented here represent a valuable tool for both quality control and the development of improved biodiesel production and purification procedures.Fil: Aguirre, Andres. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Tecnologia; Argentina. Keclon S.A,; ArgentinaFil: Cabruja, Matias Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Cabrera, Rodolfo. Unitec Bio S.A; ArgentinaFil: Eberhardt, Maria Florencia. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Tecnologia; Argentina. Keclon S.A,; ArgentinaFil: Peirú, Salvador. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Tecnologia; Argentina. Keclon S.A,; ArgentinaFil: Menzella, Hugo Gabriel. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Tecnologia; Argentina. Keclon S.A; ArgentinaFil: Rasia, Rodolfo Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; ArgentinaSpringer2014-12-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/8625Aguirre, Andres; Cabruja, Matias Ezequiel; Cabrera, Rodolfo; Eberhardt, Maria Florencia; Peirú, Salvador; et al.; A Fluorometric Enzymatic Assay for Quantification of Steryl Glucosides in Biodiesel; Springer; Journal Of The American Oil Chemists Society; 92; 1; 4-12-2014; 47-530003-021X1558-9331enginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/journal/11746/92/1info:eu-repo/semantics/altIdentifier/doi/10.1007/s11746-014-2574-3info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:52:47Zoai:ri.conicet.gov.ar:11336/8625instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:52:48.143CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A Fluorometric Enzymatic Assay for Quantification of Steryl Glucosides in Biodiesel
title A Fluorometric Enzymatic Assay for Quantification of Steryl Glucosides in Biodiesel
spellingShingle A Fluorometric Enzymatic Assay for Quantification of Steryl Glucosides in Biodiesel
Aguirre, Andres
Biodiesel
Steryl Glucosides
Fluorescence
Nadph
title_short A Fluorometric Enzymatic Assay for Quantification of Steryl Glucosides in Biodiesel
title_full A Fluorometric Enzymatic Assay for Quantification of Steryl Glucosides in Biodiesel
title_fullStr A Fluorometric Enzymatic Assay for Quantification of Steryl Glucosides in Biodiesel
title_full_unstemmed A Fluorometric Enzymatic Assay for Quantification of Steryl Glucosides in Biodiesel
title_sort A Fluorometric Enzymatic Assay for Quantification of Steryl Glucosides in Biodiesel
dc.creator.none.fl_str_mv Aguirre, Andres
Cabruja, Matias Ezequiel
Cabrera, Rodolfo
Eberhardt, Maria Florencia
Peirú, Salvador
Menzella, Hugo Gabriel
Rasia, Rodolfo Maximiliano
author Aguirre, Andres
author_facet Aguirre, Andres
Cabruja, Matias Ezequiel
Cabrera, Rodolfo
Eberhardt, Maria Florencia
Peirú, Salvador
Menzella, Hugo Gabriel
Rasia, Rodolfo Maximiliano
author_role author
author2 Cabruja, Matias Ezequiel
Cabrera, Rodolfo
Eberhardt, Maria Florencia
Peirú, Salvador
Menzella, Hugo Gabriel
Rasia, Rodolfo Maximiliano
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Biodiesel
Steryl Glucosides
Fluorescence
Nadph
topic Biodiesel
Steryl Glucosides
Fluorescence
Nadph
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Steryl glucosides (SG) are common contaminants in biodiesel that form precipitates, which form and cause problems due to fouling during transport and storage. Therefore, their quantification is necessary to assess the quality of this fuel. The methods currently available for SG analysis require expensive instrumentation, need a previous concentration step by solid-phase extraction (SPE) or are of limited use for the quantitative assessment. We developed an enzymatic method for SG quantification in biodiesel samples based on the hydrolysis of the glucoside catalyzed by a broadly specific beta glucosidase and the subsequent determination of the glucose released by the reaction. The method is non-expensive, sensitive and was adapted to 96-well format fluorescence plate reader, making it useful for the parallel assay of multiple samples. The enzymatic assay presented here represent a valuable tool for both quality control and the development of improved biodiesel production and purification procedures.
Fil: Aguirre, Andres. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Tecnologia; Argentina. Keclon S.A,; Argentina
Fil: Cabruja, Matias Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Cabrera, Rodolfo. Unitec Bio S.A; Argentina
Fil: Eberhardt, Maria Florencia. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Tecnologia; Argentina. Keclon S.A,; Argentina
Fil: Peirú, Salvador. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Tecnologia; Argentina. Keclon S.A,; Argentina
Fil: Menzella, Hugo Gabriel. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Tecnologia; Argentina. Keclon S.A; Argentina
Fil: Rasia, Rodolfo Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
description Steryl glucosides (SG) are common contaminants in biodiesel that form precipitates, which form and cause problems due to fouling during transport and storage. Therefore, their quantification is necessary to assess the quality of this fuel. The methods currently available for SG analysis require expensive instrumentation, need a previous concentration step by solid-phase extraction (SPE) or are of limited use for the quantitative assessment. We developed an enzymatic method for SG quantification in biodiesel samples based on the hydrolysis of the glucoside catalyzed by a broadly specific beta glucosidase and the subsequent determination of the glucose released by the reaction. The method is non-expensive, sensitive and was adapted to 96-well format fluorescence plate reader, making it useful for the parallel assay of multiple samples. The enzymatic assay presented here represent a valuable tool for both quality control and the development of improved biodiesel production and purification procedures.
publishDate 2014
dc.date.none.fl_str_mv 2014-12-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/8625
Aguirre, Andres; Cabruja, Matias Ezequiel; Cabrera, Rodolfo; Eberhardt, Maria Florencia; Peirú, Salvador; et al.; A Fluorometric Enzymatic Assay for Quantification of Steryl Glucosides in Biodiesel; Springer; Journal Of The American Oil Chemists Society; 92; 1; 4-12-2014; 47-53
0003-021X
1558-9331
url http://hdl.handle.net/11336/8625
identifier_str_mv Aguirre, Andres; Cabruja, Matias Ezequiel; Cabrera, Rodolfo; Eberhardt, Maria Florencia; Peirú, Salvador; et al.; A Fluorometric Enzymatic Assay for Quantification of Steryl Glucosides in Biodiesel; Springer; Journal Of The American Oil Chemists Society; 92; 1; 4-12-2014; 47-53
0003-021X
1558-9331
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/journal/11746/92/1
info:eu-repo/semantics/altIdentifier/doi/10.1007/s11746-014-2574-3
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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