Thyroid hormone-induced haemoglobin changes and antioxidant enzymes response in erythrocytes

Autores
Araujo, A. S. R.; Seibel, F. E. R.; Fernandes, T.; Llesuy, Susana Francisca; Kucharski, L.; Belló Klein, A.
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Thyroid hormones modulate haemoglobin and reactive oxygen species (ROS) production, leading to antioxidant changes. This study evaluated the antioxidant response to ROS in erythrocytes in hypothyroid and hyperthyroid rats. Wistar rats were divided into four groups: control; hyperthyroid (T4-12mgl-1 in drinking water); sham operated (simulation of thyroidectomy); and hypothyroid (thyroidectomized). Four weeks after, blood was collected and haemoglobin and T4 levels, lipid peroxidation (LPO), protein oxidation, superoxide dismutase (SOD), catalase (CAT) , glutathione S-transferase (GST) and glutathione peroxidase (GPx) activities, and total radical antioxidant potential (TRAP) were measured. SOD, CAT and GST immunocontent was evaluated. Haemoglobin levels were increased in hyperthyroid erythrocytes. LPO and carbonyls were augmented (65% and 55%, respectively) in hyperthyroid and reduced (31% and 56%, respectively) in hypothyroid group. SOD and CAT activities have not changed, as well as CAT immunocontent. TRAP was diminished in both hyperthyroid and hypothyroid groups (36% and 37%, respectively). GST activity and immunocontent, as well as GPx activity, were increased in hyper and hypothyroid rats. The data suggest that thyroid hormone changes determine ROS concentration changes and decrease of some antioxidant defences that would lead to a compensatory answer of the GST and GPx enzymes, which could be consider as credible biomarkers.
Fil: Araujo, A. S. R.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Seibel, F. E. R.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Fernandes, T.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Llesuy, Susana Francisca. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Kucharski, L.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Belló Klein, A.. Universidade Federal do Rio Grande do Sul; Brasil
Materia
Blood-Biomarkers
Gpx
Gst
Hyperthyroidism
Hypothyroidism
Oxidative Damage
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/67465
Acceder
id CONICETDig_3907212f7aae8262b1c9476a85ad1525
oai_identifier_str oai:ri.conicet.gov.ar:11336/67465
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Thyroid hormone-induced haemoglobin changes and antioxidant enzymes response in erythrocytesAraujo, A. S. R.Seibel, F. E. R.Fernandes, T.Llesuy, Susana FranciscaKucharski, L.Belló Klein, A.Blood-BiomarkersGpxGstHyperthyroidismHypothyroidismOxidative Damagehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Thyroid hormones modulate haemoglobin and reactive oxygen species (ROS) production, leading to antioxidant changes. This study evaluated the antioxidant response to ROS in erythrocytes in hypothyroid and hyperthyroid rats. Wistar rats were divided into four groups: control; hyperthyroid (T4-12mgl-1 in drinking water); sham operated (simulation of thyroidectomy); and hypothyroid (thyroidectomized). Four weeks after, blood was collected and haemoglobin and T4 levels, lipid peroxidation (LPO), protein oxidation, superoxide dismutase (SOD), catalase (CAT) , glutathione S-transferase (GST) and glutathione peroxidase (GPx) activities, and total radical antioxidant potential (TRAP) were measured. SOD, CAT and GST immunocontent was evaluated. Haemoglobin levels were increased in hyperthyroid erythrocytes. LPO and carbonyls were augmented (65% and 55%, respectively) in hyperthyroid and reduced (31% and 56%, respectively) in hypothyroid group. SOD and CAT activities have not changed, as well as CAT immunocontent. TRAP was diminished in both hyperthyroid and hypothyroid groups (36% and 37%, respectively). GST activity and immunocontent, as well as GPx activity, were increased in hyper and hypothyroid rats. The data suggest that thyroid hormone changes determine ROS concentration changes and decrease of some antioxidant defences that would lead to a compensatory answer of the GST and GPx enzymes, which could be consider as credible biomarkers.Fil: Araujo, A. S. R.. Universidade Federal do Rio Grande do Sul; BrasilFil: Seibel, F. E. R.. Universidade Federal do Rio Grande do Sul; BrasilFil: Fernandes, T.. Universidade Federal do Rio Grande do Sul; BrasilFil: Llesuy, Susana Francisca. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; ArgentinaFil: Kucharski, L.. Universidade Federal do Rio Grande do Sul; BrasilFil: Belló Klein, A.. Universidade Federal do Rio Grande do Sul; BrasilJohn Wiley & Sons Ltd2011-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/67465Araujo, A. S. R.; Seibel, F. E. R.; Fernandes, T.; Llesuy, Susana Francisca; Kucharski, L.; et al.; Thyroid hormone-induced haemoglobin changes and antioxidant enzymes response in erythrocytes; John Wiley & Sons Ltd; Cell Biochemistry And Function; 29; 5; 7-2011; 408-4130263-6484CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/cbf.1765info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/cbf.1765info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:38:43Zoai:ri.conicet.gov.ar:11336/67465instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:38:43.314CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Thyroid hormone-induced haemoglobin changes and antioxidant enzymes response in erythrocytes
title Thyroid hormone-induced haemoglobin changes and antioxidant enzymes response in erythrocytes
spellingShingle Thyroid hormone-induced haemoglobin changes and antioxidant enzymes response in erythrocytes
Araujo, A. S. R.
Blood-Biomarkers
Gpx
Gst
Hyperthyroidism
Hypothyroidism
Oxidative Damage
title_short Thyroid hormone-induced haemoglobin changes and antioxidant enzymes response in erythrocytes
title_full Thyroid hormone-induced haemoglobin changes and antioxidant enzymes response in erythrocytes
title_fullStr Thyroid hormone-induced haemoglobin changes and antioxidant enzymes response in erythrocytes
title_full_unstemmed Thyroid hormone-induced haemoglobin changes and antioxidant enzymes response in erythrocytes
title_sort Thyroid hormone-induced haemoglobin changes and antioxidant enzymes response in erythrocytes
dc.creator.none.fl_str_mv Araujo, A. S. R.
Seibel, F. E. R.
Fernandes, T.
Llesuy, Susana Francisca
Kucharski, L.
Belló Klein, A.
author Araujo, A. S. R.
author_facet Araujo, A. S. R.
Seibel, F. E. R.
Fernandes, T.
Llesuy, Susana Francisca
Kucharski, L.
Belló Klein, A.
author_role author
author2 Seibel, F. E. R.
Fernandes, T.
Llesuy, Susana Francisca
Kucharski, L.
Belló Klein, A.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Blood-Biomarkers
Gpx
Gst
Hyperthyroidism
Hypothyroidism
Oxidative Damage
topic Blood-Biomarkers
Gpx
Gst
Hyperthyroidism
Hypothyroidism
Oxidative Damage
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Thyroid hormones modulate haemoglobin and reactive oxygen species (ROS) production, leading to antioxidant changes. This study evaluated the antioxidant response to ROS in erythrocytes in hypothyroid and hyperthyroid rats. Wistar rats were divided into four groups: control; hyperthyroid (T4-12mgl-1 in drinking water); sham operated (simulation of thyroidectomy); and hypothyroid (thyroidectomized). Four weeks after, blood was collected and haemoglobin and T4 levels, lipid peroxidation (LPO), protein oxidation, superoxide dismutase (SOD), catalase (CAT) , glutathione S-transferase (GST) and glutathione peroxidase (GPx) activities, and total radical antioxidant potential (TRAP) were measured. SOD, CAT and GST immunocontent was evaluated. Haemoglobin levels were increased in hyperthyroid erythrocytes. LPO and carbonyls were augmented (65% and 55%, respectively) in hyperthyroid and reduced (31% and 56%, respectively) in hypothyroid group. SOD and CAT activities have not changed, as well as CAT immunocontent. TRAP was diminished in both hyperthyroid and hypothyroid groups (36% and 37%, respectively). GST activity and immunocontent, as well as GPx activity, were increased in hyper and hypothyroid rats. The data suggest that thyroid hormone changes determine ROS concentration changes and decrease of some antioxidant defences that would lead to a compensatory answer of the GST and GPx enzymes, which could be consider as credible biomarkers.
Fil: Araujo, A. S. R.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Seibel, F. E. R.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Fernandes, T.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Llesuy, Susana Francisca. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Kucharski, L.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Belló Klein, A.. Universidade Federal do Rio Grande do Sul; Brasil
description Thyroid hormones modulate haemoglobin and reactive oxygen species (ROS) production, leading to antioxidant changes. This study evaluated the antioxidant response to ROS in erythrocytes in hypothyroid and hyperthyroid rats. Wistar rats were divided into four groups: control; hyperthyroid (T4-12mgl-1 in drinking water); sham operated (simulation of thyroidectomy); and hypothyroid (thyroidectomized). Four weeks after, blood was collected and haemoglobin and T4 levels, lipid peroxidation (LPO), protein oxidation, superoxide dismutase (SOD), catalase (CAT) , glutathione S-transferase (GST) and glutathione peroxidase (GPx) activities, and total radical antioxidant potential (TRAP) were measured. SOD, CAT and GST immunocontent was evaluated. Haemoglobin levels were increased in hyperthyroid erythrocytes. LPO and carbonyls were augmented (65% and 55%, respectively) in hyperthyroid and reduced (31% and 56%, respectively) in hypothyroid group. SOD and CAT activities have not changed, as well as CAT immunocontent. TRAP was diminished in both hyperthyroid and hypothyroid groups (36% and 37%, respectively). GST activity and immunocontent, as well as GPx activity, were increased in hyper and hypothyroid rats. The data suggest that thyroid hormone changes determine ROS concentration changes and decrease of some antioxidant defences that would lead to a compensatory answer of the GST and GPx enzymes, which could be consider as credible biomarkers.
publishDate 2011
dc.date.none.fl_str_mv 2011-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/67465
Araujo, A. S. R.; Seibel, F. E. R.; Fernandes, T.; Llesuy, Susana Francisca; Kucharski, L.; et al.; Thyroid hormone-induced haemoglobin changes and antioxidant enzymes response in erythrocytes; John Wiley & Sons Ltd; Cell Biochemistry And Function; 29; 5; 7-2011; 408-413
0263-6484
CONICET Digital
CONICET
url http://hdl.handle.net/11336/67465
identifier_str_mv Araujo, A. S. R.; Seibel, F. E. R.; Fernandes, T.; Llesuy, Susana Francisca; Kucharski, L.; et al.; Thyroid hormone-induced haemoglobin changes and antioxidant enzymes response in erythrocytes; John Wiley & Sons Ltd; Cell Biochemistry And Function; 29; 5; 7-2011; 408-413
0263-6484
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/cbf.1765
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/cbf.1765
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons Ltd
publisher.none.fl_str_mv John Wiley & Sons Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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