Assessment of the Ferroptosis Regulators: Glutathione Peroxidase 4, Acyl-Coenzyme A Synthetase Long-Chain Family Member 4, and Transferrin Receptor 1 in Patient-Derived Endometrios...

Autores
Mielke Cabello, Lidia A.; Meresman, Gabriela Fabiana; Darici, Dogus; Carnovale, Noelia Romina; Heitkötter, Birthe; Schulte, Miriam; Espinoza Sánchez, Nancy A.; Le , Quang Khoi; Kiesel, Ludwig; Schäfer, Sebastian D.; Götte, Martin
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Ferroptosis, a novel iron-dependent form of non-apoptotic cell death, plays a pivotal role in various diseases. In the realm of endometriosis, ferroptosis is an emerging and relatively unexplored topic, characterized by inherent ambiguity regarding its physiological significance. This case-control study is a comprehensive immunohistochemical assessment of the expression and tissue distribution of established ferroptosis markers: GPX4, ACSL4, and TfR1 in endometriosis patients. Our study revealed a significant downregulation of GPX4 in stromal cells of endometriosis patients (M = 60.8% ± 42.3 versus 90.5% ± 17.2 in the control group, t (56) = -2.94, p = 0.005). This finding aligned with slightly higher iron levels detected in the blood of endometriosis patients, using hemoglobin as an indirect predictor (Hb 12.9 ± 1.1 g/dL versus 12.2 ± 1.9 g/dL in the control group; t (56) = -1.621, one-sided p = 0.055). Interestingly, there was no concurrent upregulation of TfR1, responsible for iron uptake into cells. The strongest correlations were found between GPX4 and ACSL4 as ferroptosis inhibitor and inductor, respectively. Our empirical findings provide support for the involvement of ferroptosis in the context of endometriosis. However, variances in expression patterns within stromal and epithelial cellular subsets call for further in-depth investigation.
Fil: Mielke Cabello, Lidia A.. Universitätsklinikum Münster; Alemania
Fil: Meresman, Gabriela Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Darici, Dogus. Universitätsklinikum Münster; Alemania
Fil: Carnovale, Noelia Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Heitkötter, Birthe. Universitätsklinikum Münster; Alemania
Fil: Schulte, Miriam. Universitätsklinikum Münster; Alemania
Fil: Espinoza Sánchez, Nancy A.. Universitätsklinikum Münster; Alemania
Fil: Le , Quang Khoi. Universitätsklinikum Münster; Alemania
Fil: Kiesel, Ludwig. Universitätsklinikum Münster; Alemania
Fil: Schäfer, Sebastian D.. Universitätsklinikum Münster; Alemania
Fil: Götte, Martin. Universitätsklinikum Münster; Alemania
Materia
ENDOMETRIOSIS
GENITAL DISEASES, FEMALE
BIOMARKERS
GPX4
ACSL
IRON
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/242684
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/242684
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Assessment of the Ferroptosis Regulators: Glutathione Peroxidase 4, Acyl-Coenzyme A Synthetase Long-Chain Family Member 4, and Transferrin Receptor 1 in Patient-Derived Endometriosis TissueMielke Cabello, Lidia A.Meresman, Gabriela FabianaDarici, DogusCarnovale, Noelia RominaHeitkötter, BirtheSchulte, MiriamEspinoza Sánchez, Nancy A.Le , Quang KhoiKiesel, LudwigSchäfer, Sebastian D.Götte, MartinENDOMETRIOSISGENITAL DISEASES, FEMALEBIOMARKERSGPX4ACSLIRONhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Ferroptosis, a novel iron-dependent form of non-apoptotic cell death, plays a pivotal role in various diseases. In the realm of endometriosis, ferroptosis is an emerging and relatively unexplored topic, characterized by inherent ambiguity regarding its physiological significance. This case-control study is a comprehensive immunohistochemical assessment of the expression and tissue distribution of established ferroptosis markers: GPX4, ACSL4, and TfR1 in endometriosis patients. Our study revealed a significant downregulation of GPX4 in stromal cells of endometriosis patients (M = 60.8% ± 42.3 versus 90.5% ± 17.2 in the control group, t (56) = -2.94, p = 0.005). This finding aligned with slightly higher iron levels detected in the blood of endometriosis patients, using hemoglobin as an indirect predictor (Hb 12.9 ± 1.1 g/dL versus 12.2 ± 1.9 g/dL in the control group; t (56) = -1.621, one-sided p = 0.055). Interestingly, there was no concurrent upregulation of TfR1, responsible for iron uptake into cells. The strongest correlations were found between GPX4 and ACSL4 as ferroptosis inhibitor and inductor, respectively. Our empirical findings provide support for the involvement of ferroptosis in the context of endometriosis. However, variances in expression patterns within stromal and epithelial cellular subsets call for further in-depth investigation.Fil: Mielke Cabello, Lidia A.. Universitätsklinikum Münster; AlemaniaFil: Meresman, Gabriela Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Darici, Dogus. Universitätsklinikum Münster; AlemaniaFil: Carnovale, Noelia Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Heitkötter, Birthe. Universitätsklinikum Münster; AlemaniaFil: Schulte, Miriam. Universitätsklinikum Münster; AlemaniaFil: Espinoza Sánchez, Nancy A.. Universitätsklinikum Münster; AlemaniaFil: Le , Quang Khoi. Universitätsklinikum Münster; AlemaniaFil: Kiesel, Ludwig. Universitätsklinikum Münster; AlemaniaFil: Schäfer, Sebastian D.. Universitätsklinikum Münster; AlemaniaFil: Götte, Martin. Universitätsklinikum Münster; AlemaniaMDPI2024-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/242684Mielke Cabello, Lidia A.; Meresman, Gabriela Fabiana; Darici, Dogus; Carnovale, Noelia Romina; Heitkötter, Birthe; et al.; Assessment of the Ferroptosis Regulators: Glutathione Peroxidase 4, Acyl-Coenzyme A Synthetase Long-Chain Family Member 4, and Transferrin Receptor 1 in Patient-Derived Endometriosis Tissue; MDPI; Biomolecules; 14; 7; 7-2024; 1-132218-273XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/biom14070876info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2218-273X/14/7/876info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:43:02Zoai:ri.conicet.gov.ar:11336/242684instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:43:02.955CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Assessment of the Ferroptosis Regulators: Glutathione Peroxidase 4, Acyl-Coenzyme A Synthetase Long-Chain Family Member 4, and Transferrin Receptor 1 in Patient-Derived Endometriosis Tissue
title Assessment of the Ferroptosis Regulators: Glutathione Peroxidase 4, Acyl-Coenzyme A Synthetase Long-Chain Family Member 4, and Transferrin Receptor 1 in Patient-Derived Endometriosis Tissue
spellingShingle Assessment of the Ferroptosis Regulators: Glutathione Peroxidase 4, Acyl-Coenzyme A Synthetase Long-Chain Family Member 4, and Transferrin Receptor 1 in Patient-Derived Endometriosis Tissue
Mielke Cabello, Lidia A.
ENDOMETRIOSIS
GENITAL DISEASES, FEMALE
BIOMARKERS
GPX4
ACSL
IRON
title_short Assessment of the Ferroptosis Regulators: Glutathione Peroxidase 4, Acyl-Coenzyme A Synthetase Long-Chain Family Member 4, and Transferrin Receptor 1 in Patient-Derived Endometriosis Tissue
title_full Assessment of the Ferroptosis Regulators: Glutathione Peroxidase 4, Acyl-Coenzyme A Synthetase Long-Chain Family Member 4, and Transferrin Receptor 1 in Patient-Derived Endometriosis Tissue
title_fullStr Assessment of the Ferroptosis Regulators: Glutathione Peroxidase 4, Acyl-Coenzyme A Synthetase Long-Chain Family Member 4, and Transferrin Receptor 1 in Patient-Derived Endometriosis Tissue
title_full_unstemmed Assessment of the Ferroptosis Regulators: Glutathione Peroxidase 4, Acyl-Coenzyme A Synthetase Long-Chain Family Member 4, and Transferrin Receptor 1 in Patient-Derived Endometriosis Tissue
title_sort Assessment of the Ferroptosis Regulators: Glutathione Peroxidase 4, Acyl-Coenzyme A Synthetase Long-Chain Family Member 4, and Transferrin Receptor 1 in Patient-Derived Endometriosis Tissue
dc.creator.none.fl_str_mv Mielke Cabello, Lidia A.
Meresman, Gabriela Fabiana
Darici, Dogus
Carnovale, Noelia Romina
Heitkötter, Birthe
Schulte, Miriam
Espinoza Sánchez, Nancy A.
Le , Quang Khoi
Kiesel, Ludwig
Schäfer, Sebastian D.
Götte, Martin
author Mielke Cabello, Lidia A.
author_facet Mielke Cabello, Lidia A.
Meresman, Gabriela Fabiana
Darici, Dogus
Carnovale, Noelia Romina
Heitkötter, Birthe
Schulte, Miriam
Espinoza Sánchez, Nancy A.
Le , Quang Khoi
Kiesel, Ludwig
Schäfer, Sebastian D.
Götte, Martin
author_role author
author2 Meresman, Gabriela Fabiana
Darici, Dogus
Carnovale, Noelia Romina
Heitkötter, Birthe
Schulte, Miriam
Espinoza Sánchez, Nancy A.
Le , Quang Khoi
Kiesel, Ludwig
Schäfer, Sebastian D.
Götte, Martin
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ENDOMETRIOSIS
GENITAL DISEASES, FEMALE
BIOMARKERS
GPX4
ACSL
IRON
topic ENDOMETRIOSIS
GENITAL DISEASES, FEMALE
BIOMARKERS
GPX4
ACSL
IRON
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Ferroptosis, a novel iron-dependent form of non-apoptotic cell death, plays a pivotal role in various diseases. In the realm of endometriosis, ferroptosis is an emerging and relatively unexplored topic, characterized by inherent ambiguity regarding its physiological significance. This case-control study is a comprehensive immunohistochemical assessment of the expression and tissue distribution of established ferroptosis markers: GPX4, ACSL4, and TfR1 in endometriosis patients. Our study revealed a significant downregulation of GPX4 in stromal cells of endometriosis patients (M = 60.8% ± 42.3 versus 90.5% ± 17.2 in the control group, t (56) = -2.94, p = 0.005). This finding aligned with slightly higher iron levels detected in the blood of endometriosis patients, using hemoglobin as an indirect predictor (Hb 12.9 ± 1.1 g/dL versus 12.2 ± 1.9 g/dL in the control group; t (56) = -1.621, one-sided p = 0.055). Interestingly, there was no concurrent upregulation of TfR1, responsible for iron uptake into cells. The strongest correlations were found between GPX4 and ACSL4 as ferroptosis inhibitor and inductor, respectively. Our empirical findings provide support for the involvement of ferroptosis in the context of endometriosis. However, variances in expression patterns within stromal and epithelial cellular subsets call for further in-depth investigation.
Fil: Mielke Cabello, Lidia A.. Universitätsklinikum Münster; Alemania
Fil: Meresman, Gabriela Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Darici, Dogus. Universitätsklinikum Münster; Alemania
Fil: Carnovale, Noelia Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Heitkötter, Birthe. Universitätsklinikum Münster; Alemania
Fil: Schulte, Miriam. Universitätsklinikum Münster; Alemania
Fil: Espinoza Sánchez, Nancy A.. Universitätsklinikum Münster; Alemania
Fil: Le , Quang Khoi. Universitätsklinikum Münster; Alemania
Fil: Kiesel, Ludwig. Universitätsklinikum Münster; Alemania
Fil: Schäfer, Sebastian D.. Universitätsklinikum Münster; Alemania
Fil: Götte, Martin. Universitätsklinikum Münster; Alemania
description Ferroptosis, a novel iron-dependent form of non-apoptotic cell death, plays a pivotal role in various diseases. In the realm of endometriosis, ferroptosis is an emerging and relatively unexplored topic, characterized by inherent ambiguity regarding its physiological significance. This case-control study is a comprehensive immunohistochemical assessment of the expression and tissue distribution of established ferroptosis markers: GPX4, ACSL4, and TfR1 in endometriosis patients. Our study revealed a significant downregulation of GPX4 in stromal cells of endometriosis patients (M = 60.8% ± 42.3 versus 90.5% ± 17.2 in the control group, t (56) = -2.94, p = 0.005). This finding aligned with slightly higher iron levels detected in the blood of endometriosis patients, using hemoglobin as an indirect predictor (Hb 12.9 ± 1.1 g/dL versus 12.2 ± 1.9 g/dL in the control group; t (56) = -1.621, one-sided p = 0.055). Interestingly, there was no concurrent upregulation of TfR1, responsible for iron uptake into cells. The strongest correlations were found between GPX4 and ACSL4 as ferroptosis inhibitor and inductor, respectively. Our empirical findings provide support for the involvement of ferroptosis in the context of endometriosis. However, variances in expression patterns within stromal and epithelial cellular subsets call for further in-depth investigation.
publishDate 2024
dc.date.none.fl_str_mv 2024-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/242684
Mielke Cabello, Lidia A.; Meresman, Gabriela Fabiana; Darici, Dogus; Carnovale, Noelia Romina; Heitkötter, Birthe; et al.; Assessment of the Ferroptosis Regulators: Glutathione Peroxidase 4, Acyl-Coenzyme A Synthetase Long-Chain Family Member 4, and Transferrin Receptor 1 in Patient-Derived Endometriosis Tissue; MDPI; Biomolecules; 14; 7; 7-2024; 1-13
2218-273X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/242684
identifier_str_mv Mielke Cabello, Lidia A.; Meresman, Gabriela Fabiana; Darici, Dogus; Carnovale, Noelia Romina; Heitkötter, Birthe; et al.; Assessment of the Ferroptosis Regulators: Glutathione Peroxidase 4, Acyl-Coenzyme A Synthetase Long-Chain Family Member 4, and Transferrin Receptor 1 in Patient-Derived Endometriosis Tissue; MDPI; Biomolecules; 14; 7; 7-2024; 1-13
2218-273X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/biom14070876
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2218-273X/14/7/876
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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